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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which adsorption was measurement at 450 nm using a microplate reader (Multiscan FC, Thermo Scientific).
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (#S181BH-500, Biowest) and 1% penicillin-streptomycin (#15140122; Gibco). Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9 ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Microbiology 2021Quote: ... the -10 and -35 consensus sequences of the J23119 promoter and the lpp-sgRNA chimera (Table S1) was synthesized by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA) and cloned in pCRISPR-sacB using AvrII and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Microbiology 2022Quote: ... The titer of the applied FVT virome was approximately 5.4 × 109 virus-like particles (VLP)/mL (Figure S12 and Table S1) for both 1st and 2nd inoculation and was evaluated by epifluorescence microscopy stained by SYBR™ Gold (Thermo Scientific, cat. no. S11494) as previously described (http://dx.doi.org/10.17504/protocols.io.bx6cpraw) ...
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bioRxiv - Zoology 2024Quote: ... with optimized primers designed using Geneious Pro Bioinformatics Software (Supplement Table S1) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, United States) using PowerUPTM SYBR® Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments were amplified using appropriate primer pairs (Table S1) and Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific, Walthman, MA, United States). Linear fragments were purified on agarose gels and inserted into pGMCS backbone linearized by PCR amplification with appropriate primers ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Microbiology 2021Quote: 5×106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E-and recombinant plasmid (pS-SARS, pS-MERS, pS-D614, pS-G614, pS-Variant1, or pS-Variant2) using Lipofectamine 3000 Transfection Reagent (Invitrogen, Rockville, MD) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Exosomes were isolated from 300 µl of plasma from 30 healthy individuals and 30 subjects with SARS-CoV2 infection using “Total Exosome Precipitation Reagent” from Invitrogen (Carlsbad, CA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Microbiology 2023Quote: ... CoV RNA was detected using a pancoronavirus nested PCR with primers targeting the conserved RdRp gene of CoVs as previously published (Watanabe et al., 2010) and using the Platinum II Taq Hot-Start DNA Polymerase (Thermo Fisher Scientific). After PCR ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was diluted 1:1 with cold H2O and loaded onto a C-tag Affinity Column (ThermoFisher) equilibrated with C-tag Binding Buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Desalted peptides were then labeled with 10-plex Tandem-mass-tag (TMT) reagents following manufacturer’s instructions (Thermo Scientific). The samples were then reconstituted in volume of 20 mM ammonium formate (pH 10 ...
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bioRxiv - Microbiology 2019Quote: ... anti-V5 (1:50 or 1:100; V5 Tag Monoclonal Antibody, Cat. Nr. R960-25, Thermo Fisher Scientific) or self-made antibodies raised against GlCHC (dilution 1:1000 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Protein products with HA tag were detected using 1:500 anti-HA antibody (Life Technologies, Catalog number: 326700), NURF-1.D isoform with FLAG tag was detected using 1:1000 PIERCE ANTI-DYKDDDDK antibody (Life Technologies ...
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bioRxiv - Plant Biology 2019Quote: The antibodies used for these ChIP experiments were as follows: GFP Tag Antibody (A-11120, Thermo Fisher Scientific), anti-H3 (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... as per the commercial protocol) and 5 μL of fluorescent tag kit (Alexa Fluor® succinimidyl esters, Invitrogen, Molecular Probes® ...
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bioRxiv - Neuroscience 2020Quote: ... https://emb.carnegiescience.edu/drosophila-gateway-vector-collection) and PMT-pcBlast-Nterm GFP tag (CuSO4 Inducible promoter, Thermo Fisher V413020).
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bioRxiv - Plant Biology 2021Quote: ... “Protein extracted” with T7 tag antibodies were incubated with protein A containing magnetic beads (Dyna beads, Thermo fisher) for 2 hours at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Full-length EWS/FLI and mutants (all containing amino-terminal 3xFLAG-tags) were cloned into pMSCV-Hygro (Invitrogen) with sequence details provided in Additional file 1 ...
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bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
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bioRxiv - Cell Biology 2021Quote: ... The LAP tag from pIC113 vector (Cheeseman and Desai, 2005) was subcloned into pcDNA5.0/FRT/TO vector (Invitrogen) to make the pcDNA5.0/FRT/TO-LAP-N vector ...
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bioRxiv - Cancer Biology 2020Quote: ... His-tagged GOT2 protein was immunoprecipitated using the His-tag isolation and pull-down Dynabeads system (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... for immunofluorescence staining or eight-well Lab-Tek chamber coverglass for double-tag analysis (Thermo Fisher Scientific, #155411), and fixed with 4% formaldehyde in PBS for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... Individual samples were then labeled with isobaric tags using commercially available TMTsixplex (Thermo Fisher Scientific, P/N 90061) kits ...
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bioRxiv - Cancer Biology 2022Quote: Isobaric labelling of peptides was performed using the 16-plex tandem mass tag (TMT) reagents (Thermo Fisher Scientific) (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2022Quote: ... Tags and deletions were confirmed by colony PCR (Master Mix PCR Hot Start II Phire Green, Thermo Fisher) or microscopy analysis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We followed standard immunoblot procedures using primary antibodies raised against the Xpress tag (ThermoFisher R910-25, 1:5,000), and anti-mouse IgG HRP-conjugated secondary antibodies (Jackson ImmunoResearch 715-035-150 ...
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bioRxiv - Neuroscience 2019Quote: The plasmid containing a codon-optimized neurotactin cDNA with 2xHA tag was obtained from GeneArt (ThermoFisher Scientific, USA). C ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were resuspended in MS-grade water and labeled with tandem mass tags (TMT 10-plex; Thermo Fisher). Samples were resolved by nano-electrospray ionization on an Orbitrap Fusion Lumos MS instrument (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Genetics 2022Quote: ... Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific) or the Pierce HA Tag IP/Co-IP Kit (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... thus leading to a fusion of the emdyp open reading frame with the anti-V5 epitope tag (Invitrogen) and a hexahistidine tag ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 1hr with a 1:1000 dilution of GST-Tag antibody (MA4-004, Thermo Fisher) in blocking buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tetherin localisation in cells was detected by staining cells with anti-HA-tag rabbit monoclonal IgG (Thermo Fisher) diluted in 0.2% Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... The presence of the Cas9 protein was tested using antibody against the V5 tag (ref R960-25, Invitrogen). Secondary antibodies used for Western blot were labelled with HRP (Jackson Immuno Research) ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG M2 or HA-tag antibody at 4 °C overnight before precipitated with magnetic Protein G beads (Thermofisher) for 2 hours at 4 °C followed by 3 washes with RIPA buffer.
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bioRxiv - Molecular Biology 2024Quote: ... The polyhistidine tag in the insoluble fraction was probed with the SuperSignal West HisProbe Kit (ThermoFisher Scientific 15168) according to the manufacturer’s recommendation with a modification ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...