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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 127 pg of REBOV Vp40-pGEM-T Easy and 7.7 pg of MERS-CoV UpE-p GEM-T Easy using 2X Verso hot-start PCR buffer (Thermo Scientific). The reaction was set up in 15 μl final volume and the thermal cycling was as follows ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells (CRL-11268™, ATCC®. Gaithersburg, Maryland, US) were cultured in DMEM high glucose (31966-021, Gibco™, Thermo Fisher, UK), supplemented with 10% FBS (16140-071 ...
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bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
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bioRxiv - Genomics 2022Quote: Frozen HEK293 or K562 cell pellets were lysed in 1 mL lysis buffer supplemented with 5 µl protease inhibitor cocktail (Thermo Fisher cat# 87786) and 10 µl Rnase inhibitor (NEB cat# M0314B ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... SK-Hep1, Huh7, MHCC-97H, and HEK293 cells were cultured in DMEM (HyClone, Logan, UT, USA) supplemented with 10% FBS (Gibco, Rockville, MD, USA), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells stably expressing CMV:DOR-APEX2 were created as previously described and maintained in 1:1000 G418/Geneticin (Thermo Fisher Scientific, Cat # 10131035) (Lobingier et al ...
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bioRxiv - Immunology 2023Quote: ... Gregoire Lauvau’s lab) and HEK293 Phoenix Ampho Packaging Cells (provided by Prof. Andrew Ward) were cultured in complete DMEM medium (Gibco, Cat#10569-010) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC; CRL-1573™) were cultured in complete DMEM (cDMEM) in Nunc EasYFlask T75 cell-culture flasks (Thermo Scientific; cat # 156499) and housed in CO2 incubator (5% CO2 ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: The plasmids coding for pro-BMP9 and pro-BMP10 were co-transected in equal amounts into HEK293 cells (expi293, Invitrogen/Thermo Fisher Scientific) and after five days ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2µl lysis buffer with 1U/µl Rnase Inhibitor (RRI) and ERCC spike in mix at a 1:20 dilution (Ambion Cat #4456740). Plates were promptly sealed (Thermo Fisher Scientific #AB0626) ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 ng of total RNA were mixed with 1 µL of a 1:10 dilution of ERCC RNA Spike-In Mix controls (Thermo Fisher 4456740). ERCC mix 1 was added to HEK293T and HEK293T-Nsp1 and mix 2 was added to HEK293T-Nsp2 samples ...
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bioRxiv - Synthetic Biology 2021Quote: ExpiCHO-S cells (6E6cells/mL) were transfected with 1ug/mL of Spike protein DNA22 using the ExpiCHO™ Expression System Kit (Gibco #A29133) and shaken at 37°C and 8% CO2 in a 50mL culture ...
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bioRxiv - Developmental Biology 2020Quote: ... 500ng of RNA was used to prepare RNA-seq libraries and spiked-in with ERCC Exfold Spike-in mixes (Thermo Fisher, 4456739). RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Preparation kit (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured in a Dulbecco’s Modified Eagle’s medium (DMEM) containing 10% FBS and transfected by pLV-Spike using a calcium phosphate kit (ThermoFisher, Waltham, MA, USA). The control virus with VSV-G as the tropism and expression of mCherry was generated by co-transfection of pLV-mCherry and pMD2.G vector (Addgene ...
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bioRxiv - Biochemistry 2020Quote: ... different domains or subunits of the Spike protein and the extracellular domain of ACE2 were produced in Expi293F cells (Thermo Fisher Scientific). Expi293F cells were cultured at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The final Spike protein concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Final proteins were dispensed in aliquots of 0.5 ml each and stored at -80°C prior to use.
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bioRxiv - Genomics 2022Quote: ... Human cell lysate and mouse cell lysate (as a spike-in internal control) were combined (∼4:1 ratio) and incubated with protein A or G Dynabeads (Thermo Fisher Scientific) conjugated with the relevant antibodies for 14 h at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Reads that did not map to rRNA were then mapped with STAR (2.5.3a) to the full reference genome (UCSC build GRCm38/mm10) (including the RNA spike-in control sequence ERCC92, ThermoFisher cat. no. 4456740) using the following parameters ...
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bioRxiv - Molecular Biology 2019Quote: ... Nanopore libraries were prepared from 1 μg poly(A)+ RNA combined with 1 μl undiluted ERCC RNA Spike-In mix (Thermo Fisher Scientific) using the nanopore DRS Kit (SQK-RNA001 ...
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bioRxiv - Immunology 2020Quote: ... Fragmented chromatin from G1-arrested cells with or without spike-in was precleared with 40 μl Dynabeads Protein A (ThermoFisher Scientific, #10002D) and then incubated with Rad21 (Abcam ...
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bioRxiv - Molecular Biology 2021Quote: 10 ng total RNA containing 5 pM ath-miR-159a spike-in was reverse-transcribed using Taqman Advanced miRNA cDNA synthesis kit (Applied Biosystems #A28007) and miRNAs were detected using specific probes for ath-miR159a (478411_mir) ...
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bioRxiv - Systems Biology 2020Quote: RNA-Seq libraries were prepared from 65 ng of total RNA and 1 μL of 1:5000 (v/v) dilution of ERCC ExFold RNA Spike-In Mix 1 (Thermo Fisher Scientific) with TruSeq® Stranded mRNA Library Prep for NeoPrep™ instrument as per the NeoPrep Library Prep System Guide on the instrument (Illumina).
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bioRxiv - Biochemistry 2020Quote: The ACE2 and Spike proteins were expressed and purified from Expi293 BirA cells according to established protocol from the manufacturer (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequence and gene annotation of the mitochondrial genome assembly (NCBI GenBank, AY659991.1) and RNA spike-in control sequences (ERCC, Ambion; for quality control) were included as well.
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 30ml of ExpiCHO-S cells at 6M cells/mL were transfected with 1ug/mL of the spike WT using the ExpiCHOTM Expression System Kit (Gibco, catalog # A29133) following the manufacturer’s Standard protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Sequence and gene annotation of the mitochondrial genome assembly (NCBI GenBank, AY659991.1) and RNA spike-in control sequences (ERCC, Ambion; for quality control) were included as well ...
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bioRxiv - Genomics 2023Quote: ... and External RNA Control Consortium (ERCC) spike-in transcripts were added to M8 and D6 samples at manufacturer recommended amounts (4456740, Thermo Fisher Scientific) 13 ...
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bioRxiv - Immunology 2023Quote: ... The ectodomain of ancestral SARS-CoV-2 spike (GenBank: MN908947.3) was expressed as previously described.10 The protein was purified from FreeStyle 293-F cells (Thermo Fisher Scientific, USA) using affinity chromatography followed by size exclusion chromatography ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix (Invitrogen, cat# 4456740. 1:1,000 dilution). All libraries were sequenced on the Illumina NovaSeq 6000 system.
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bioRxiv - Cell Biology 2020Quote: ... T1249-Fc were expressed by transient transfection in 293-6E (CNRC) or Expi293 (ThermoFisher Scientific) cells and purified from transfected cell supernatants using a HiTrap MabSelect SuRe column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... absorbance at 405 nm was measured in a MultiSkan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... ECD region of mouse CLEC2 was cloned into mammalian expression vector pFuse-rIgG-Fc (Invitrogen). The construct was used to stable transfect HEK-293T cells with PEI method ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... 10% FCS and 100 units / ml of penicillin and 100 μg / ml of streptomycin (Gibco). U87MG ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were blocked with anti-mouse CD16/32 (Fc-block, Invitrogen, San Jose, CA, USA) for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11 ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-rabbit IgG Fc secondary antibody (Invitrogen, Cat. No. 31463).
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). MLV P30 protein within the pseudovirus capsid was detected using a rabbit anti-MLV-P30 polyclonal antibody (Origene ...
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bioRxiv - Microbiology 2021Quote: ... and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen, Cat. No. 31437). Beta-actin was used as an internal control.
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bioRxiv - Molecular Biology 2020Quote: ... Absorbance at 570 nm was measured using a spectrophotometer (Multiskan FC Microplate Photometer, Thermo Fisher). A standard curve was generated using standard glycogen solution in the reaction ...
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Absorbance was measured at OD450 nm using a Multiskan™ FC Microplate Photometer (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Cells were infected with the specified viruses and MOIs in DMEM without FCS (Life Technologies) for 1h at 37.0°C (Cts24 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco) for 3 hours before fixation for 15 minutes (3.7% FA and 0.5% triton X-100) ...
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bioRxiv - Molecular Biology 2022Quote: ... and then blocked in PBS supplemented with 20% foetal calf serum (FCS, Thermo Fisher Scientific) and 20% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ... and were used after freezing in Fetal Calf Serum (FCS) (Invitrogen, Waltham, Massachusetts, United States) containing 10% DMSO (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Absorbance at 405 nm was read on a Multiskan FC plate reader (Thermo Scientific, USA).