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Citations for Thermo Fisher :
4801 - 4850 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 mRNA was transcribed from pCS2-Cas9 plasmid using mMessage mMachine SP6 Kit (Ambion) and purified by lithium chloride precipitation ...
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bioRxiv - Genomics 2021Quote: ... HEK293T.rKSHV219 cells were reverse transfected with 0.8 μg plasmid DNA using Lipofectamine2000 (Life Technologies) following the manufacturer’s instructions and seeded in 12 or 24-well plates for analysis by live-cell imaging or qRT-PCR as indicated ...
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bioRxiv - Immunology 2021Quote: ... Plasmids containing genes of interest were used to transform E.coli BL21 (DE3) Star (Invitrogen). Recombinant proteins were induced upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 0.8×106 cells were transfected with 1.5µg plasmid DNA using Lipofectamine 2000 (11668019, Invitrogen). Selection with 2µg/ml puromycin (P8833 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmid DNA was quantified using a NanoDrop ND-1000 spectrophotometer (ThermoFisher Scientific, Waltham, MA). Plasmids were serially diluted and used as standards for quantitative real-time PCR (29).
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bioRxiv - Microbiology 2022Quote: T293 cells were transfected with plasmid DNA (pGIPZ shPDE12) using Lipofectamine 2000 reagent (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The vectors were subsequently harvested with the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... The pET30a(+) vectors were harvested using the GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific) at a concentration of 96.7 ng ·μL-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Linearized plasmids were in vitro transcribed using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA polyadenylated using Poly(A ...
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bioRxiv - Neuroscience 2022Quote: ... Linearized plasmids were in vitro transcribed using mMESSAGE mMACHINE T7 kit (Ambion/Thermo Fisher), the resulting mRNA polyadenylated using Poly(A ...
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bioRxiv - Cell Biology 2022Quote: ... or plasmid DNA and siRNA co-transfection experiments were performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... then CRISPR/Cas and donor plasmids were transfected using Lipofectamine 2000 (Thermo Fisher Scientific). Two days after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... and the indicated plasmids were co-transfected using 2ug DNA and Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: RNAs were transcribed from XbaI-linearized plasmids (MEGAscript T7 Transcription kit; Thermo Fisher Scientific), purified (RNeasy MinElute columns ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μg of LRG sgRNA expression plasmid was digested with FastDigest Esp3I (ThermoFisher, FD0454) and gel purified using QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with plasmid using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher). For nucleofection of RNP complexes 4 μl crRNA (100 μM ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.3 μg target plasmid using 36 μL Lipofectamine 3000 (Thermo Fisher Scientific; L3000008). DNA was added to 500 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified from single colonies and verified to contain the desired inserts (Invitrogen). The protocol used for generation of the MaMTH reporter cell lines was described previously (Saraon et al. ...
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bioRxiv - Cell Biology 2022Quote: COS-7 cells were transfected with the plasmid using Lipofectamine 2000 (Thermo Fisher Scientific). Transfection conditions are described in each experiment ...
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bioRxiv - Cell Biology 2022Quote: ... and NBPXa-CtermLoxP/HA constructs were first cloned into plasmid backbone (Geneart, Thermofisher Scientific), containing a multi-cloning site with SacII ...
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bioRxiv - Cell Biology 2022Quote: ... The linearized plasmids were treated with 0.2 mg/mL proteinase K (Invitrogen, 25530-015), purified with phenol–chloroform treatment and ethanol precipitation ...
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bioRxiv - Cell Biology 2022Quote: ... 1-2 µg of total plasmid DNA was transfected using lipofectamine 3000 (Thermo Scientific). Cells were trypsinized and replated onto No 1.5 coverglass the following day and analyzed ∼12-16 h later by indirect immunofluorescence ...
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bioRxiv - Genetics 2022Quote: ... 1 μg of plasmid DNA was diluted in 125 μl Opti-MEM (Life Technologies). 4 μl Lipofectamine 2000 were diluted in 125 μl Opti-MEM (Life Technologies ...
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bioRxiv - Genetics 2022Quote: ... 10 µg pX330 plasmids containing gRNA for TP53 and 10 µg ApaLI (Thermo Fisher)-linerized TP53-ReCOIN targeting donors were electroporated into about 1 million PFFs ...
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bioRxiv - Neuroscience 2022Quote: ... The plasmids were transfected to HEK cells in OPTI-MEM using lipofectamine 2000 (Invitrogen) at 70-80% confluency.
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bioRxiv - Neuroscience 2022Quote: ... neurons were transfected with plasmid using Lipofectamine 2000 (Invitrogen, catalog no. 11-668-019) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Successful plasmids were quantified with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen) and diluted from 108 copies to 100 copies ...
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bioRxiv - Microbiology 2022Quote: ... The restriction enzymes and ligase used in plasmid construction were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and then inserted into plasmid pSMT3-LxEGFP restricted with BamHI and HindIII (Thermo Scientific) using T4 DNA ligase ...
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bioRxiv - Neuroscience 2022Quote: ... AAV.DUX4 mutant constructs.V5 or pCINeo (transfection control plasmid) using Lipofectamine 2000 reagent (Thermo Scientific). Cells were harvested 24 hours post-transfection in RIPA buffer containing 10 mM Tris-Cl (pH 8.0) ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of luciferase reporter plasmid pPASPORT constructs was carried out using Lipofectamine 2000 (Thermofisher) according to the user manual ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected in equimolar ratios in high glucose DMEM (Gibco™, Dublin, Ireland) supplemented with 2% FBS and 1% antibiotic/antimycotic solution ...
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bioRxiv - Plant Biology 2022Quote: ... the amiRMYB68 was introduced in the pKGWFS7 destiny plasmid using the Gateway® (Invitrogen) recombination system,
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bioRxiv - Cell Biology 2022Quote: ... All plasmids used for injection were purified using a DNA Miniprep Kit (PureLink, Invitrogen), or a DNA midiprep kit (Macherey Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... WT mESCs were transfected with these plasmids and pgk-puro using Lipofectamine 2000 (Thermofisher), transfected cells were enriched using transient puromycin (Sigma Adrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transiently transfected with the indicated plasmids using Lipofectamine 2000 or 3000(Invitrogen) as per manufacturer’s instruction.
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bioRxiv - Developmental Biology 2024Quote: ... pME-EYFP and p3E-polyA plasmids using Gateway LR Clonase II Enzyme Mix (Invitrogen). We generated miR-144/451::EYFP transgenic line using Tol2 transposon system (REF) ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transfected into HEK293-T or LNCaP cells using Lipofectamine 2000 (Invitrogen) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Cell Biology 2024Quote: ... Plasmids for Cas9 and homology repair were transfected to cells with Lipofectamine 2000 (Invitrogen). Successful C-terminal knock-in cells were identified by fluorescence activated cell sorting (FACS ...
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bioRxiv - Immunology 2024Quote: ... equal mass of heavy and light chain plasmids were transfected into Expi293F cells (Gibco) per manufacturer’s instructions and harvested 6 days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). After 24 h ...
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bioRxiv - Genetics 2024Quote: ... HEK-293 cells were transfected with respective DNA plasmids and Lipofectamine 2000 (Life Technologies) for 48 hours before lysis ...
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bioRxiv - Cell Biology 2024Quote: All cells were transfected with plasmids using the Lipofectamine™ 3000 Transfection Reagent (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: Plasmid maintenance and amplification was carried out using Escherichia coli TOP10© (ThermoFisher Scientific). All PrP constructs were generated by standard PCR cloning techniques and are based on the coding region of mouse PRNP (GenBank accession number M18070 ...
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bioRxiv - Microbiology 2023Quote: ... plasmids were mixed in 500 µl of Opti-MEM I reduced serum medium (Gibco) and incubated with 18 µl of TransIT-LT1 Reagent (Mirus ...
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bioRxiv - Molecular Biology 2024Quote: ... they were transfected with the reporter plasmid in the presence of jetPEI reagent (Invitrogen). They were incubated for 14 h and then rinsed with fresh medium ...
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 300 ng of plasmid were transfected using Lipofectamine 3000 (ThermoFisher, L3000015), following the manufacturer’s protocol ...