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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... Ligated plasmids were electroporated into One-Shot® Top10 cells (Life Technologies, Carlsbad, US). This step was used to maximize the library diversity due to One-Shot® Top10 cells’ high competency ...
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bioRxiv - Microbiology 2021Quote: ... the cells were transfected with 15ug spike expression plasmid with Lipofectamine LTX (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μg of each plasmid was combined with 10 μl Lipofectamine 2000 reagent (Invitrogen) in 1 ml Opti-MEM and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were transfected with plasmid DNA using Lipofectamine 2000 (Invitrogen, Cat# 11668-019) and GBM cells were transfected with plasmid DNA using Lipofectamine 2000 Stem reagent (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The final plasmid DNA concentration was determined with the Nanodrop (Thermo Scientific NanoDrop 2000c).
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bioRxiv - Neuroscience 2021Quote: ... N2a cells were transfected with full-length plasmids using Lipofectamine 3000 (Invitrogen Cat#L3000015) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and hKCa1.1 were prepared from linearized plasmids using the mMESSAGE mMACHINE T7 kit (Ambion) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA probes were generated from the linearized plasmids using T7 RNA polymerase (Thermo Fisher) and digoxygenin- or fluorescein-labeled nucleotides (Roche) ...
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bioRxiv - Neuroscience 2020Quote: ... Transient transfection of siRNA oligonucleotides or plasmids was performed using Lipofectamine™ RNAiMAX (Invitrogen) in Opti-MEM (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: 70%-confluent cells were transfected using Lipofectamine RNAimax (siRNA) or Lipofectamine 2000 (plasmid) (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... cells at 70% confluency were transfected with plasmid of interest using Lipofectamine 2000 (Invitrogen) or TransIT-X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... COS7 cells were transfected with plasmid DNAs by using TurboFect transfection reagents (Thermo Fisher). HeLa ...
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bioRxiv - Biophysics 2021Quote: ... Plasmids bearing GlyR constructs were transiently transfected into HEK293T cells by Lipofectamine 3000 (Invitrogen) following manufacturer’s protocol at different α2:β DNA ratios (1:0 ...
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bioRxiv - Biophysics 2021Quote: Plasmids bearing GlyR constructs were transiently transfected into HEK293T cells by Lipofectamine 3000 (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting amplicon was cloned into pcDNA3.1/Hygromycin plasmid vector (Invitrogen, Waltham, MA, USA) using ApaI and AflII ...
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bioRxiv - Cancer Biology 2020Quote: ... 1μg psiCheck2 empty vector or psiCheck2_Fabp1eCLIP plasmids were mixed with Lipofectamine 3000 (L3000001, Invitrogen) and P3000 enhancer reagent in Opti-MEM medium.10μL of transfection mixture was added to each well and incubated at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were transfected using Lipofectamine 3000 reagent according to the instruction’s guidelines (ThermoFisher Scientific). After 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and Lipofectamine 2000 (ThermoFisher) in a 1:3 reagent:DNA ratio per well ...
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bioRxiv - Cell Biology 2021Quote: ... EGFP-ORP5B and Mito-BFP plasmids were transfected with of lipofectamine 2000/well (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting plasmid was amplified in Escherichia coli TOP10 chemically competent cells (Life Technologies). DNA was isolated using a NucleoBond Xtra Maxi kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2021Quote: ... prior to being recombined into expression plasmids by LR cloning (Life Technologies, 11791-020). Plasmid constructs were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... cells at 70% confluency were transfected with plasmid of interest using Lipofectamine 2000 (Invitrogen) or TransIT-X2 (Mirus Bio ...
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bioRxiv - Cell Biology 2021Quote: ... Then the plasmid was transfected into CHO_Flp cells using lipofectamine® 3000 Reagent (Invitrogen), and the positive clones will be screened out over ten days in the presence of Hygromycin B (50 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... pcDNA5/FRT/TO USP7 plasmids were co-transfected with pOG44 (Invitrogen, Thermo Fisher Scientific) into inducible FlpIn T-Rex U2OS cells (gift from Prof Steven C ...
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bioRxiv - Cell Biology 2021Quote: ... pcDNA5/FRT/TO USP7 plasmids were co-transfected with pOG44 (Invitrogen, Thermo Fisher Scientific) into inducible FlpIn T-Rex U2OS cells (gift from Prof Steven C ...
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bioRxiv - Plant Biology 2020Quote: ... The pGY1-GW plasmid was constructed using the Gateway™Vector Conversion System (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... acetivorans replicating plasmids by retrofitting with pAMG40 [46] Gateway BP Clonase II kit (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: We packaged lentivirus by transfecting HEK293T cells with LV-MAX lentiviral packaging plasmids (Gibco) and the LTR-containing transfer vector of interest using Lipofectamine 3000 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... the px330 plasmid backbone was digested with BbsI restriction enzyme (FastDigest BpiI: ThermoFisher Scientific). Guide RNA oligos containing BbsI overhangs were then phosphorylated and annealed ...
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bioRxiv - Neuroscience 2020Quote: ... HEK 293T were triple transfected with viral vector and packaging plasmids pLP/VSVG (Invitrogen) and psPAX2 (AddGene) ...
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bioRxiv - Neuroscience 2020Quote: ... plasmids were transformed into chemocompetent bacteria (E. coli Top10™, Thermo Fisher Scientific, TFS), which were generated like previously described70 ...
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bioRxiv - Immunology 2021Quote: ... and subcloned into the pCR2.1 plasmid using the TOPO-TA cloning Kit (Thermo Fisher). Plasmids were prepared using the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Neuroscience 2019Quote: ... These plasmids were transfected along with Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, USA) into HEK293 T cells ...
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bioRxiv - Neuroscience 2019Quote: Full-length rat GluA1 and GluA2 cDNAs were subcloned into pcDNA3 (plasmid vector, Invitrogen) to generate pc3-GluA1 and pc3-GluA2 ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR amplicon was cloned into a pCR2.1-TOPO plasmid (Thermo Fisher Scientific – K450002). Sense (S ...
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bioRxiv - Plant Biology 2020Quote: ... a recombineering cassette was amplified from plasmid pLM099 (Phusion Hotstart II polymerase, ThermoFisher Scientific) using primers containing 50 bp homology arms ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were digested using PvuII fast digest enzyme (Thermo Fisher Scientific, Waltham, MA, USA) and compared with the digestion pattern of the pST76AIS1X::VioABCDE plasmid.
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bioRxiv - Microbiology 2021Quote: ... and 3.33 µg of the S-protein coding plasmid using Lipofectamine 2000 (ThermoFisher Scientific) accordinhg to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 ng of pGL3 reporter plasmid along with 10 ng of either pcDNA3.1+ (Invitrogen) or pcDNA-Tat and 100 ng expression vector was co-transfected ...
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bioRxiv - Microbiology 2020Quote: ... before ligation to plasmid using the Zero Blunt TOPO PCR Cloning Kit (ThermoFisher Scientific); all steps were according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or pcDNA plus RFP plasmid(57) Hippocampal cultures were transfected with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the siRNA duplexes or overexpression plasmids were transfected into cells using Lipofectamine-2000 (Invitrogen) for 48 h ...
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bioRxiv - Biochemistry 2020Quote: ... the GeneJET Plasmid Miniprep Kit and the GeneJET Gel Extraction Kit from Thermo Scientific were used ...
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bioRxiv - Cancer Biology 2020Quote: ... 1,5 μg plasmid DNA was transfected using the Lipofectamine 2000 transfection reagent (Life Technologies) according to manufacturer’s protocol ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... and subcloned into the pCR2.1 plasmid using the TOPO-TA cloning Kit (Thermo Fisher). Plasmids were prepared using the QIAprep Spin Miniprep Kit (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry-UBQLN2 (mCh-UBQLN2) plasmids were diluted in Opti-MEM Reduced Serum Medium (ThermoFisher) and allowed 20 minutes to form complexes with Trans-IT transfection reagent (Mirus) ...
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bioRxiv - Cell Biology 2020Quote: ... Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668, Invitrogen). Histidine-tagged ubiquitin (pCI-His-hUbi ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection of plasmid DNA was performed with Lipofectamine 3000 and P3000 reagent (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: The gene encoding for ADR1a was inserted into the pRSET plasmid (Thermo Fisher Scientific), between the NdeI and XhoI restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids were delivered to the cells by using Plus Reagent and Lipofectamine LTX (Invitrogen) following the manufacturer’s protocol ...