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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and 1 ng of CMV-Rluc reporter plasmids (78) using Lipofectamine 3000 (Invitrogen L3000015).
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bioRxiv - Plant Biology 2024Quote: ... genes were inserted into the ChampionTM pET300-NT-DEST plasmid (ThermoFisher Scientific, Waltham, MA), before being transformed into Rosetta(DE3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 600 ng of each sgRNA plasmid were mixed together with 0.5 μL BpiI (ThermoFisher), 1 μL of 10X Buffer G (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and EGFP-LAMP1 genes into the doxycycline-inducible lentiviral expression plasmid pTRIPZ (Thermo Fisher). Lentiviruses were produced by transfecting HEK293T cells with plasmids encoding VSV-G ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-4 μg of plasmid was mixed with 500 μL Opti-Mem (Gibco 31985070), after which 2.5 μL Plus Reagent and 8 μL Lipofectamine LTX (Invitrogen 15338100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with plasmids (described previously [21]) using the NeonTM Transfection System (Invitrogen). A total of 5×106 cells in 10-cm tissue culture plates were transfected with 25 µg of PX458 plasmid containing cloned sgRNA using the electroporation method (1100 V ...
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bioRxiv - Genomics 2023Quote: Plasmids were transfected into HEK293XT cells using Lipofectamine 3000 (Thermo Fisher Scientific, Cat # L3000015). To prepare HEK cells for transfection ...
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bioRxiv - Bioengineering 2023Quote: ... which was achieved by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Cat.#. K0502). Plasmid isolation protocol was applied according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... gene fragments were amplified from plasmids by PCR using Platinum Pfx DNA polymerase (ThermoFisher). Constructs were assembled by Gibson assembly using the Gibson Assembly® Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The plasmids were then transformed in One Shot TOP10 chemically competent cells (Thermofisher, #C404010), amplified ...
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bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... Each resultant plasmid was co-transfected with ViraPower™ Lentiviral Packaging Mix (ThermoFisher # K496000) into 293FT cells to make virus particles ...
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bioRxiv - Developmental Biology 2023Quote: ... to enrich cultures for mini-prep (Gene Jet Plasmid Miniprep kit k0503, Thermo Fisher). Purified plasmids were sent for sequencing (Eurofins ...
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bioRxiv - Immunology 2023Quote: ... and the fused cDNA was cloned into the pcDNA3.1 expression plasmid (Invitrogen, Calsbad, CA). For visiualization of EROS ...
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bioRxiv - Plant Biology 2023Quote: ... CDS were then integrated into the pDONR207 plasmid using BP clonase II mix (Thermofisher). pDONR207 plasmids containing PpSMXL CDS were submitted to PCR-mediated mutagenesis to obtain ΔRGK/RT versions.
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bioRxiv - Plant Biology 2024Quote: ... Their PCR products were cloned into pJET1.2 blunt plasmids (Thermo Fisher Scientific, Massachusetts, USA) and sequenced.
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bioRxiv - Developmental Biology 2023Quote: ... Linearized plasmids were subjected to invitro transcription using a SP6 Transcription Kit (Invitrogen AM1340). The resulting mRNA was purified (Total RNA Kit I ...
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bioRxiv - Cell Biology 2024Quote: ... All plasmid DNA was quantified by UV-Vis absorbance using a NanoDrop spectrophotometer (ThermoFisher). All sequences were verified by sequencing performed by Eurofins Genomics (Eurofins Genomics LLC ...
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bioRxiv - Genetics 2024Quote: ... 10.8ug of plasmid was transfected onto 106 cells using the 100uL Neon system (ThermoFisher) with a single ...
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bioRxiv - Developmental Biology 2024Quote: ... and transient transfection with plasmid DNAs was performed using Lipofectamin 3000 (Thermo Fisher Scientific), when cells reached between 60-70% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... GAG-POL and pInducer lentiviral plasmids carrying the transgene using Lipofectamine 3000 (Thermo Fisher). After 24h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with plasmids using the Neon Transfection System (Life Technologies, Carlsbad, CA), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were performed using the LipofectamineTM 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500).
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bioRxiv - Genetics 2024Quote: ... coli under appropriate conditions and purified using GeneJET plasmid miniprep kit (Thermo Fisher Scientific) using included instructions ...
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bioRxiv - Immunology 2024Quote: ... and pLP2 plasmids into HEK293T cells at 80–90% confluency using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... FaDu cells were transfected with 500 ng of plasmid according to manufacturer’s instructions (ThermoFisher) and cells were single cell sorted 48 hours after transfection.
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transiently transfected with lipofectamine 2000 (Life Technologies, catalog no. 11668-027) into cancer cells as stated in the figures.
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bioRxiv - Biophysics 2024Quote: ... Plasmid constructs were transfected into the cells by using Lipofectamine 2000 reagent (Invitrogen, 11668019). After 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Flat-Bottom Microplate with pcDNA vector plasmids using PLUS and Lipofectamine Reagents (ThermoFisher Scientific). Plasmids contained genes for human or zebrafish TLR4 complex components and Renilla luciferase enzyme under constitutively active promoters ...
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bioRxiv - Cancer Biology 2024Quote: ... was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific) and added to the cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... combining a total of ∼1.0 μg of plasmid DNAs with serum-free OptiMEM (Gibco) to a final volume of 100 μL ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid transfections were performed using Lipofectamine™ 3000 transfection reagent (Thermo Fisher, #3000-008) or ViaFect (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The guide RNA-Cas9 plasmid was transfected into A549 cells using Lipofectamine 3000 (ThermoFisher) and a cell line-specific protocol provided by ThermoFisher (58) ...
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bioRxiv - Immunology 2024Quote: ... and specific sgRNA-cloned plasmids using Lipofectamine 3000 (Thermo-Fisher) and OptiMEM medium (Gibco) according to the manufacturer’s instructions for 72 hours ...
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bioRxiv - Microbiology 2024Quote: ... HeLa cells were transfected with pcDNA3.1-GFP plasmids using Lipofectamine LTX (Thermo Fisher Scientific). Cells were fixed with 4% formaldehyde 18h post-transfection and the nucleus was stained using DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... ASO and plasmids at 60–80% confluency was performed using Lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The plasmids were transfected into HEK293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Plasmids were transfected in cells with JetPrime (Polyplus) transfection reagent or oligofectamine (ThermoFisher Scientific) in 6-well plates per manufacturer’s protocol and for all shRNA plasmids ...
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bioRxiv - Genomics 2024Quote: ... The plasmid lentiMPRA library was electroporated into MegaX DH10B T1R Electrocomp Cells (Invitrogen, C640003) using the Gemini X2 (2.0 kV ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from human stromal cells using Trizol reagent according the Manufacturer’s instructions (Invitrogen) and genomic DNA was removed using rDNase I RNase-free (Takara) ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were incubated with a 1:200 dilution of human cTnT primary mouse antibody (ThermoFisher) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: HeLa (human cervix adenocarcinoma) cells were cultured for 48 hours in RPMI-1640 (Gibco, 61870-010) + 5% FBS (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with 100 or 200 nM Accell Human Rab11A siRNA SMARTPool (Thermo Fisher Scientific) with 0.1 % FBS ...
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bioRxiv - Genetics 2021Quote: ... and then the quantification was conducted using the Quantifiler Human DNA Quantification Kit (Thermo Fisher Scientific) on an Applied Biosystems 7500 Real-time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: The WT and RIF1 KO human stem cells were cultured in StemFlex media (Thermo Fisher A3349401) on Geltrex (Thermo Fisher A1413302) ...
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bioRxiv - Cell Biology 2020Quote: ... Then the HUVEC monolayer was primed by 20 ng/ml human TNF-a (Life technologies PHC3015) for 4-6 h ...
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bioRxiv - Microbiology 2020Quote: ... labeled and hybridized to separate arrays using the GeneChip Human Exon 1.0 ST Array (Affymetrix, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: All of the Silencer Select siRNA oligos targeting human GBP mRNA were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified human eIF2 (100 nM) was incubated with 100 nM BODIPY-FL-GDP (Thermo Fisher Scientific) in assay buffer (20 mM HEPES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... SAOS-2 human osteosarcoma cells were obtained from ATCC and grown in McCoy’s 5A medium (Invitrogen) supplemented with 15% FBS ...