Labshake search
Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Biotinylated hACE2-Fc was prepared using EZ-Link™ Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... to each well and the plates read at 450 nm using a Multiscan FC Plate Reader (Thermo Scientific). ELISA results were presented as normalised OD450 with the mean OD450 values of blank wells subtracted from the sample well values.
-
bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
bioRxiv - Immunology 2020Quote: ... Caco-2WT and Caco-2ΔHk2 cells were cultured in MEM with 20% (v/v) FCS purchased from Gibco/Life Technologies ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 10% Foetal Calf Serum (FCS, Biosera – FB 1001/500) and 2 mM GlutaMAX™ (Gibco – 35050061) at 37°C with 5% CO2.
-
bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450nm (OD450) using an accuSkan FC microwell plate reader (Thermo Fisher Scientific).
-
bioRxiv - Immunology 2020Quote: ... and lymphocytes were resuspended in complete RPMI (cRPMI- RPMI + 10% FCS + 2% Penicillin-Streptomycin + 1% L-glutamine (Gibco)) ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... was used to measure the m6A level in total RNA by MultiSkan FC microplate reader (Thermo Fisher, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies ...
-
bioRxiv - Physiology 2024Quote: ... The isolated cells were plated (0.1 % gelatine-coated T75 flasks, approx. 1.0×107 cells) and cultured in 10 % FCS-containing DMEM (Gibco). Medium was changed every 3 days ...
-
bioRxiv - Biophysics 2023Quote: ... Both iPSCs classes were cultured on 0.1% gelatin-coated dishes in FCS/LIF medium (DMEM+Glutamax (61965059, Gibco), 15% fetal calf serum (Pansera ES ...
-
bioRxiv - Microbiology 2023Quote: ... Polyclonal goat anti-pig immunoglobulin G (IgG) Fc Secondary Antibody HRP (Invitrogen by Thermo Fisher Scientific, Waltham, USA) was diluted 1:15.000 in blocking buffer and strips were incubated for 1 h at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... supplemented in media (DMEM with 10% FCS and penicillin, Life Technologies, final conc. 50 U/mL of penicillin) for 2 hours at 37° C ...
-
bioRxiv - Developmental Biology 2023Quote: ... Bones were flushed using a 26-gauge needle of a 1-ml syringe with HBSS+2% FCS (Gibco). BM clumps were re-suspended by gentling aspirating with a 19-gauge needle ...
-
bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
-
bioRxiv - Bioengineering 2023Quote: ... Both cell lines were maintained in RPMI-1640 media supplemented with 10% heat-inactivated FCS (Gibco Life Technologies) and 2mM GlutaMAX ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated with FC block for 30 minutes and stained with anti-MECA79 (53-6036-82, Invitrogen), anti-GP38 (127423 ...
-
bioRxiv - Biochemistry 2023Quote: ... Plates were incubated at 30 °C and OD600 was measured using a Multiskan FC Microplate Photometer (Thermo Fisher). For the evaluation of chemical compounds ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
-
bioRxiv - Immunology 2023Quote: ... The idiotype antibodies and Fc- fusion protein were conjugated in house with Dylight650 antibody labelling kits (Thermo Fisher). T cell surface phenotype was assessed using the following antibodies:
-
bioRxiv - Bioengineering 2023Quote: ... the brain sections were incubated with both WA2.22 Fc fusion (100 nM) and AT8 (1:200 dilution, Invitrogen) in M ...
-
bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS, Biowest Riverside) and 1% penicillin/streptomycin (Thermo Fisher Scientific), after incubation with Fc receptor blocking reagent (Miltenyi Biotech) ...
-
bioRxiv - Microbiology 2023Quote: Cells were washed with FACS buffer (0.2% BSA, 1 mM in PBS) before blocking with Fc block (Invitrogen) and incubation with eF780 live/dead viability stain (eBioscience ...
-
bioRxiv - Microbiology 2023Quote: ... followed by three washes with 1% FCS/PBS and secondary staining using Alexa-546-anti-rabbit (Invitrogen, A11035), Alexa-488-anti-mouse (Invitrogen ...
-
bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: Fc blocker CD16/32 (clone 93, Invitrogen #14-0161-85), Epcam-BV421 (clone G8.8 ...
-
bioRxiv - Cancer Biology 2023Quote: ... were grown in DMEM (Dulbecco’s Modified Eagle’s Medium, Hyclone) supplemented with 10% (v/v) FCS (Fetal Calf Serum, Gibco), 3.7 g/l sodium bicarbonate ...
-
bioRxiv - Cancer Biology 2023Quote: Viably frozen samples obtained from patients diagnosed with AML or normal CD34+ hematopoietic progenitor cells were thawed in 37°C water bath as fast as possible and placed immediately in 20% FCS Iscove’s Modified Dulbecco’s Medium (Gibco IMDM ...
-
bioRxiv - Cancer Biology 2023Quote: ... suspended in 20μl sterile complete media (MEM-Alpha (Biological Industries)) supplemented with 10% Fetal Calf Serum (FCS) (Gibco) and 1% L-Glutamine/Penicillin/Streptomycin (Biological Industries) ...
-
bioRxiv - Immunology 2024Quote: ... and absorbance was measured at 450 nm and 570 nm using the Multiskan FC Microplate Photometer (Thermo Scientific). To correct for optical imperfections ...
-
bioRxiv - Molecular Biology 2024Quote: In order to block non-specific Fc-mediated interaction,1 μg of normal Rabbit IgG (ThermoFisher Cat: 31235) is added to the cell sample and incubated for 10 minutes at room temperature ...
-
bioRxiv - Plant Biology 2024Quote: ... and the absorbance at 750 nm was measured using a plate reader (Multiskan FC, ThermoFisher, Waltham, MA, USA). Total polyphenols content was expressed as gallic acid equivalent per gram of watermelon flesh sample.
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with GlutaMAX [Gibco] supplemented with 5% fetal bovine calf serum (FCS; [Gibco]) and 50 μg/mL gentamicin [Gibco] ...
-
bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated with an appropriate HRP-conjugated secondary antibody (IgG Fc Goat anti-Rabbit, HRP, Invitrogen 31463 ...
-
bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
-
bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: The human anti-DNP and anti-WTA GlcNAc-β-4497 monoclonal antibodies were produced recombinantly in human Expi293F cells (Life Technologies) as described before (21) ...
-
bioRxiv - Cell Biology 2022Quote: ... the human organoids were cultured with 1:1 mix of Human IntestiCult™ OGM Basal Medium and DMEM/F-12 (ThermoFisher) in the presence of 100 IU Penicillin and 100 μg/ml Streptomycin ...
-
bioRxiv - Bioengineering 2020Quote: Human adiponectin was measured in the plasma of NSG mice was measured using a human-specific adiponectin ELISA from Invitrogen (KHP0041).
-
bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
-
bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: The U2OS (human bone osteosarcoma epithelial, female) cell, and HEK293T cells (human embryonic kidney epithelial, female) were cultured in DMEM (Life Technologies) with high glucose in 10% FBS (fetal bovine serum ...
-
bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
-
bioRxiv - Immunology 2021Quote: ... Other monoclonal human anti-DNP and anti-WTA IgG of the four IgG subclasses were produced recombinantly in human Expi293F cells (Life Technologies) as described before 51 with minor changes ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
-
bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
-
bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...