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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Emulsion PCR and Ion Sphere Particles (ISP) enrichment were done using the Ion PGM Hi-Q Kit for 200bp (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg of purified GST-MKLP1620-858 proteins (wild type and AQA mutant) bound to beads were phosphorylated in vitro with 2 μg of recombinant His-tagged Aurora B (Thermo Fisher) as described10,27 and then washed three times with 500 μl of phosphatase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Biochemistry 2020Quote: ... 0.2 mg of His-SUMO-SecA purified from untreated cells was bound to 100 μl Ni-NTA agarose beads (Life Technologies). The beads were incubated with 500 μl of lysis buffer or buffer containing 2 mM sodium azide for 10 minutes and washed three times with 500 μl lysis buffer ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... from a cDNA library and cloned into pcDNA™3.1/His expression vectors (Catalog #V38520, Life Technologies Co., Grand Island, NY). Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170 ...
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bioRxiv - Microbiology 2021Quote: ... The sequencing products were purified using 75% isopropanol and resuspended in 12 μl of Hi-Di™ Formamide (Thermo Fisher Scientific). After loading on the Applied Biosystems® 3130 Genetic Analyzer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells after incubation were washed with FACS buffer and incubated with anti-his Alexa Fluor 647 antibody (Thermo Fisher Scientific) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: 500 ng to 2000 ng of Hi-C library DNA or genomic DNA library were concentrated using a vacuum concentrator (Savant SPD 2010, Thermo Scientific) and then re-suspended in 5 μl dH2O ...
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bioRxiv - Microbiology 2020Quote: ... We then resuspended the cell pellet in 9.5mL PBS/2%HI-FBS for counting using the Countess Cell Counting system (Thermo Fisher Scientific). We aliquoted 0.5 mL for Seq-Well ...
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bioRxiv - Microbiology 2021Quote: ... Serum supernatant was collected as described above and incubated with a pellet of 900 μl Dynabeads™ His-Tag Isolation & Pulldown (Invitrogen) equilibrated in wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... experiments were performed in total reaction volume of 10μl with qPCRBIO SyGreen Blue Mix Hi-ROX (PCR BIOSYSTEMS) reagent on 384-wells plates (Axygen) using Viia7 (Thermo Fisher Scientific) instrument in standard conditions.
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His6- tagged SPOC domains were purified by affinity chromatography using HisTrap HP column (Cytiva) or His-Pur Ni-NTA resin (Thermo Scientific) equilibrated in 25 mM Tris-Cl pH 7.4 ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Cell Biology 2022Quote: ... the growth media was switched to differentiation media (DMEM supplemented with 5% heat-inactivated horse serum (HI-HS; Gibco/ThermoFisher Scientific) and 1% P/S ...
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bioRxiv - Molecular Biology 2022Quote: Insect or bacterially expressed proteins were purified by His-tag purification using hiTRAP FF columns (GE) or HisPur Resin (Thermo Scientific), followed by His tag removal by Tobacco Etch Virus (TEV ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
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bioRxiv - Microbiology 2020Quote: ... samples were sequenced on an Ion PGM Sequencer with Ion PGM Hi-Q View Sequence Solutions (Ion Torrent Life Technologies, USA).
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Cell Biology 2019Quote: ... the serum was collected then affinity purified using His-CSAG1 linked to the amino link plus coupling resins (Thermo scientific, #20501) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and Enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; Thermofisher Scientific). The Ion Proton reads (FASTQ files ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... Emulsion PCR and enrichment of template-positive particles were performed using an Ion PGM Hi-Q OT2 Kit (Thermo Fisher Scientific) and the Ion OneTouch 2 system (Life Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; A26433, Thermofisher Scientific). Sequencing reads were trimmed with Prinseq (160 ...
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bioRxiv - Developmental Biology 2020Quote: ... Emulsion PCR and enrichment was performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (A26434, Thermofisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length ...
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bioRxiv - Pathology 2021Quote: ... Equal amounts of GST-tagged TaBln1 or GST (negative control) were mixed with TaCam3-His and incubated at 4°C for 2 h with GST beads (Thermo Scientific). The beads were collected and washed five times using the GST Protein Interaction Pull down Kit (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The resulting product was digested with BamHI and PmeI and cloned into the BamHI/PmeI sites of pEF6/myc-His (ThermoFisher Scientific). The plasmid obtained was named pEF-POLH ...
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bioRxiv - Biochemistry 2021Quote: ... the N-terminal His-tagged protein was purified in a single step protocol using HisPur Ni-NTA resin (Thermo Fisher Scientific), equilibrated at RT ...
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bioRxiv - Bioengineering 2021Quote: ... 1:1000 mouse anti-his capture antibody in PBS was coated onto Immulon 2 HB 96-well plates (Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
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bioRxiv - Microbiology 2020Quote: ... and 293T were cultured in high-glucose DMEM (HyClone) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, ThermoFisher Scientific), 100 U/ml penicillin-G ...
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bioRxiv - Immunology 2021Quote: ... Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher). The resulting enriched library pool was subsequently sorted using a Sony SH800 Cell Sorter ...
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bioRxiv - Immunology 2021Quote: ... HI) containing S gene variants were transfected into Drosophila S2 cells using the Lipofectamine LTX with PLUS reagent (Invitrogen, Carlsbad, CA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... the human BBS2 ORF was PCR amplified with gene specific primers and sub-cloned into the pCDNA3.1V5/His TOPO TA vector (Invitrogen, Carlsbad, CA). The GeneArt Site-Directed Mutagenesis system (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Cancer Biology 2022Quote: ... p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies). ShPlk3s (shRNA-A ...
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bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
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bioRxiv - Bioengineering 2022Quote: Hi-Def B8 medium was prepared by adding Hi-Def B8 aliquots (Defined Bioscience #LSS-201, San Diego, CA, USA) to DMEM/F12 (ThermoFisher #11320033) along with 1% antibiotic-antimycotic ...
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bioRxiv - Biochemistry 2022Quote: ... and Pcdh15 EC 1-2 with 6x His and GFP tag at the C-terminus were transfected and expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific) using the standard prescribed protocol ...
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bioRxiv - Microbiology 2022Quote: His-GspD and His-PilQOlut were expressed in Escherichia coli BL21 cells and purified according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD ...
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bioRxiv - Immunology 2022Quote: The extracellular region of the HLA-DPA1*02:01 and HLA-DPB101:01 chains were separately cloned into pMT\BiP\V5-His A (ThermoFisher scientific). The alpha chain construct harbors the acidic leucine zipper and terminates by a 6His-tag ...
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bioRxiv - Plant Biology 2022Quote: ... Each sgRNA was introduced into the shuffle vectors by overlap PCR with Q5 Hi-Fidelity polymerase followed by digestion of the original vector with DpnI (Thermo Scientific). A destination vector harboring UBQ10 promoter ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and mixed end-over-end at 4°C for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...