Labshake search
Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... pMT-yki-HA was generated by cloning Yki-RG and HA tag into pMT-V5-His vector (Invitrogen, Cat# V412020). The Y281A ...
-
bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
-
bioRxiv - Cell Biology 2024Quote: All eukaryotic expression plasmids for pUL71 and pUL71 homologues were generated in the backbone of pEF1/Myc-His C expression vector (Invitrogen) by cloning using restriction enzymes ...
-
bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
-
bioRxiv - Immunology 2024Quote: Sera competition ELISA assay plates were coated with PBS containing 250 ng of anti-6x His tag monoclonal antibody (Invitrogen) and left covered overnight at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... C2C12 cells transfected with EGFP-HRas WT/C181S/C184S in 35mm dishes were treated with 15d-PGJ2-Biotin (5 µM) in DMEM Hi Glucose medium (Gibco) supplemented with 1% Penicillin-streptomycin-Glutamine (Gibco ...
-
bioRxiv - Bioengineering 2024Quote: ... Antibodies were purified by gravity column using Protein G Sepharose 4 Fast Flow Resin (Cytiva) and proteins containing His tags were purified on Ni-NTA Agarose (R90101 Invitrogen).
-
bioRxiv - Biochemistry 2024Quote: Bacmids containing 6×His-MBP-TEV-nsp12 variants were generated using the Bac-to-Bac™ baculovirus expression system (Invitrogen). Sf9 cells were transfected with the bacmid DNA and baculovirus-containing cell supernatants were harvested 5 days after transfection ...
-
bioRxiv - Biophysics 2023Quote: ... The proteins were transferred from SDS-PAGE gel to the polyvinylidene difluoride membranes by western blotting and were detected using anti-His(C-term)-HRP antibodies (Invitrogen) as described previously (80).
-
bioRxiv - Molecular Biology 2023Quote: ... Experiments were carried out using 8 μL of SETDB1-3TD (residues 196-403, N-terminal His tag) and 20X concentration Sy-pro Orange dye (5000X DMSO stock, ThermoFisher) and 2 uL of ligand for a final concentration of 20 μM of protein and 200 μM of ligand with 1.1% of DMSO ...
-
bioRxiv - Neuroscience 2023Quote: ... Sequences of full-length Dprs and DIPs with an N-terminal V5 tag were cloned into the pMT/BiP/V5-His A vector (Invitrogen) for expression in S2 Drosophila cells for PLC cleavage assay or into the pAcGP67 baculovirus transfer vector (BD Biosciences ...
-
bioRxiv - Biochemistry 2022Quote: The region coding for the full-length Hermes transposase (Uniprot Q25438, with the mutations Q2E and K128G) was cloned into pBAD/Myc-His (Invitrogen) in such a way that the recombinant protein was not tagged ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 1% FBS (CPS Serum, Cat. No. FBS-500-HI) and 1X penicillin/streptomycin (Gibco, Cat. No. 15140-122), followed by centrifugation in an Eppendorf® centrifuge (model 5810 R ...
-
bioRxiv - Immunology 2023Quote: ... Both cell lines were cultured in DMEM supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) and 100 U/mL penicillin-streptomycin (Gibco). Cells were incubated at 37°C with 5% CO2.
-
bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
-
bioRxiv - Microbiology 2022Quote: ... Infected Vero cell monolayers were immediately overlaid with DMEM 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500). R ...
-
bioRxiv - Developmental Biology 2023Quote: Peaks enriched in neural crest samples were cloned from genomic DNA using standard PCR methods using AccuPrime Hi-Fidelity Taq polymerase (Invitrogen). Primers used to amplify target enhancer sequences are provided in the Key Resources table (Table S1) ...
-
bioRxiv - Biochemistry 2023Quote: ... A total of 2 mg of lysate per condition together with 25 μL of DynabeadsTM for His-Tag isolation (Invitrogen) were incubated with His6-AtLEGβ or the His6-proAtLEGβ-C211A dead mutant or lysate alone for an hour at room temperature with rotation ...
-
bioRxiv - Microbiology 2023Quote: ... All host defense systems identified in the various screens were placed under the araBAD promoter (arabinose inducible) in the pBAD Myc/His A vector (Invitrogen). Conjugative allelic exchange was carried out using R6K-origin pKEK2201 (kanamycin ...
-
bioRxiv - Molecular Biology 2023Quote: ... and restriction enzyme sites for NotI and EcoRV were introduced for cloning into the pcDNA4 myc/His backbone (Thermo Fisher). For NARS2-Apex2 cloning ...
-
bioRxiv - Neuroscience 2023Quote: ... respectively) were amplified by PCR and inserted into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific, Waltham, MA). Fragments of genomic DNA upstream of the first methionine codon (2677 bp ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein samples were then separated by SDS-PAGE gels and transferred to the PVDF membrane for either immunoblotting with His antibody (Invitrogen) or staining with Ponceau S ...
-
bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2024Quote: ... His-Smt3-Cysless GG-FAT10 (FAT10C0) was gene synthesized and subcloned into a pET28 vector by GeneArt (Thermo Fisher Scientific), the mutations are C7T ...
-
bioRxiv - Biochemistry 2024Quote: ... The diluted reaction was then transferred to a tube containing 80 µg of pre-washed Dynabeads His-Tag Isolation and Pulldown magnetic beads (Invitrogen) and incubated on a roller for 5 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... and DsRed were cloned into pcDNA6 vector between the XhoI and XbaI sites (pcDNA6/Myc-His B; Invitrogen, Waltham, MA) with a poly(A)-tail of 84 nucleotides (Wakai et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... in imaging medium (custom; DMEM High-glucose [Gibco] without Riboflavin and Phenolred containing 10% [w/w] FCS [Gibco] ...
-
bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
-
bioRxiv - Genomics 2020Quote: ... supplemented with 10% FCS (Gibco™ Newborn Calf Serum [heat inactivated], of New Zealand origin; Thermo Fisher Scientific), 5% AA (Gibco® MEM) ...
-
bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
-
bioRxiv - Bioengineering 2021Quote: The purified Fc fusion baits were captured on 96-well protein-G coated plates (Thermo Fisher Scientific 15131). The biotinylated baits were immobilized on 96-well streptavidin-coated plates (Thermo Fisher Scientific 15500) ...
-
bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
-
bioRxiv - Cancer Biology 2019Quote: ... the two layers were covered with liquid RPMI 1640 medium containing 10% CS-FCS (Gibco® Life Technologies). The liquid top layer with or without 100 nM T3 was changed every 3 days ...
-
bioRxiv - Microbiology 2021Quote: ... ATCC (Cat# CRL-3216) were maintained with growth media (DMEM supplemented with 10% fetal calf serum (FCS, Invitrogen) and 100 U/ml penicillin and 0.1 mg/ml streptomycin (P/S ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 μM of FGFR2-Fc (Curebio) was pre-incubated with 0 ∼ 10 μM of BSA (Thermo fisher scientific) or 1 ∼ 10 μM of AIMP1 (Curebio ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... the copy number was quantified independently by performing FCS using Alexa Fluor 568 NHS ester (Thermo Fisher Scientific) to measure the confocal volume and using the cells that transiently-express mCherry alone to convert fluorescence intensity to the concentration.
-
bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were maintained at 37 °C under humidified 5% CO2 in DMEM medium supplemented with 10% FCS (Invitrogen) and 2 mM glutamine ...
-
bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco], 1 × Penicillin/Streptomycin [Gibco] and 1 × BME Vitamins [Sigma-Aldrich] and adjusted to pH 5.25 ...
-
bioRxiv - Microbiology 2020Quote: ... spun down at 1,000 × g for 10 min and pooled in equal proportions in Grace’s medium (Grace’s insect media [Sigma-Aldrich] with 4.2 mM NaHCO3, 10% FCS [Gibco] ...
-
bioRxiv - Immunology 2019Quote: ... Antibody staining was performed in PBS + 1% FCS for 30min at 4°C for live-dead marker (Invitrogen), α-CD8 (RPA-T8 ...
-
bioRxiv - Immunology 2019Quote: RMA and RMA/s thymoma cells (cultured in Iscove’s modified Dulbecco’s medium supplemented with 10% FCS; Life Technologies) were labeled with Cell Trace Blue or Cell Trace Violet ...
-
bioRxiv - Cell Biology 2020Quote: ... and the interface containing stromal cells was washed in DMEM/F12/ 10% fetal calf serum (FCS, Invitrogen, USA) and 1% primocin (Life Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... Absorbance was measured at 450 nm with Multiskan™ FC Filterbased Microplate Photometer (Thermo Scientific, Waltham, MA, USA).
-
bioRxiv - Zoology 2021Quote: ... Cells were then resuspended in L-15 (Leibovitz) medium containing 10% heat-inactivated fetal calf serum (FCS, Gibco) and antibiotics (penicillin–streptomycin ...
-
bioRxiv - Cell Biology 2020Quote: MDA-MB231 and HeLa cells were cultured in 4.5 g/L glucose DMEM supplemented with 10% FCS (Gibco), 10 U/mL penicillin (Gibco ...