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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... pNL4-3 or pMSMnG were washed and lysed in 1× NuPAGE LDS sample buffer (NP0007, Thermo Fisher Scientific) containing 2% (v/v ...
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bioRxiv - Bioengineering 2022Quote: Cultured neurons were washed 3 times with 1 mL of pre-warmed serum free Neurobasal medium (Gibco, 21103049) supplemented with 4% B-27 (Gibco ...
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bioRxiv - Biophysics 2023Quote: ... in 3% BSA with a 1:50 ratio and 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) were employed ...
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bioRxiv - Bioengineering 2022Quote: Cultured neurons were washed 3 times with 1 mL of pre-warmed serum free Neurobasal medium (Gibco, 21103049) supplemented with 4% B-27 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 × 108 asynchronised TIG-3-20 cells (per time point) were labeled with 20 μM EdU (Thermo Fisher) for 11 min ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with appropriate Alexa Fluor secondary antibodies for 3 h at room temperature (1:250, Invitrogen). Sections were mounted on glass slides and coverslipped using DAPI-Fluoromount-G (Southern Biotech).
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bioRxiv - Molecular Biology 2023Quote: ... Mix 1 contains 3 siRNAs (CliniSciences, CRH7929) and Mix 2 contains two siRNAs (ThermoFisher Scientific, 1299001 and 4392420). As a negative control ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Ligations were performed at a vector: insert mass ratio of 3:1 using T4 DNA ligase (Thermo Scientific) at 16 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of 1 M imidazole and 30 µl of Ni-NTA agarose beads (Thermo Scientific, MA, USA) were added to the mixture and incubated for 30 min at 4-8°C ...
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bioRxiv - Neuroscience 2024Quote: ... or RGS14 (n=3, 1:500, mouse anti-RGS14 #75-170, Neuromab)/goat anti-mouse IgG2a (Life Technologies).
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bioRxiv - Genetics 2024Quote: ... for 1-3 hours at 37C and subsequently cleaned up with GeneJet PCR clean up kit (Thermo Scientific) and resuspended in H2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Mitochondrial proteins were solubilized with 1% digitonin resolved on 4-16% or 3-12% NativePAGE Novex gels (Invitrogen). For blotting ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg total DNA was precomplexed with 3 µg lipofectamine 2000 in 0.2 ml Opti-MEM (ThermoFisher 51985091) for ≥ 5 min before adding to the cells in 2 ml media ...
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bioRxiv - Molecular Biology 2024Quote: ... Analytes were separated using Hypercarb Porous Graphitic Carbon HPLC Column (1 mm × 100 mm, 3 μm, Thermo Scientific) at a constant flow rate of 40 μL/min with MS buffer A (0.1% formic acid (v/v ...
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bioRxiv - Biophysics 2024Quote: ... 10 µM Rhod-3 AM in T-HBSS containing 2.5 mM Probenecid and 1× PowerLoadTM (Thermo Fisher Scientific). These loading solutions (2 ml ...
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bioRxiv - Cancer Biology 2024Quote: PANC-1 and Calu-3 cells were transfected with Silencer™ Select negative control siRNA (Invitrogen, USA, 4390843), Silencer™ Select HOXB6 siRNA (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µg Cas9 vector and 1 µg individual gRNA vector were simultaneously transfected with Lipofectamine™ 3000 (ThermoFisher) into STHdhQ7/111 cells cultured in a 6-well plate (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were treated as indicated in addition to 1 μM cell viability dye YOYO™-3 Iodide (Invitrogen) before immediately subjecting the plate to real-time cell-death analysis ...
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bioRxiv - Cell Biology 2024Quote: ... 2012) consisted of a 3:1 ratio of DMEM supplemented with 9% foetal bovine serum (FBS) (10270106, Gibco) and 1x penicillin/streptomycin with Ham’s F12 (21765 ...
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bioRxiv - Neuroscience 2023Quote: ... with a makeup flow of 0.15 ml/min of the mobile phase (acetonitrile with 0.1 % formic acid in ultrapure water with 0.1 %formic acid) and directed to the triple-quadrupole mass spectrometer (API 4000, Applied Biosystems, Foster City, CA, USA). The acquisition was run in multi-reaction mode (MRM ...
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bioRxiv - Pathology 2022Quote: ... Pythocholic acid and Tauro-conjugated pythocholic acid were purified using HyperSep C8 solid phase extraction (SPE) cartridge (Thermo Scientific, Waltham, MA, Cat # 60108-309) and silica gel chromatography (Figure 4 A) ...
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bioRxiv - Molecular Biology 2021Quote: ... The dialyzed rMNs were then labeled with either Alexa Fluor 488 (Green-Fluorescent) or Alexa Fluor 647 (Red-Fluorescent) with succinimidyl ester labeling kits (ARES DNA Labeling Kit, Invitrogen, Cat A21665 and A21676). The fluorescent labelled rMN were concentrated by spin-dialysis.
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bioRxiv - Systems Biology 2021Quote: The relative mitochondrial transmembrane potential (ΔΨm) was measured using the membrane-potential-dependent fluorescent dye TMRE (Tetramethylrhodamine, Ethyl Ester, Perchlorate) (Molecular Probes, Thermo Fisher Scientific)[87] ...
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bioRxiv - Cell Biology 2022Quote: ... the confocal volume was determined by performing FCS using a dye with known diffusion coefficient and concentration (Alexa Fluor 488 NHS ester; Thermo Fisher Scientific for mEGFP). To convert fluorescence intensity to the concentration ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... media was removed and cells were incubated with standard bath solution (SBS) containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; Thermo Fisher Scientific, Paisley, UK) and 0.01% pluronic acid for 1 h at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... Fluorescently labeled Fabs were made by conjugating with either Alexa Fluor 488 or Alexa Fluor 647 NHS Ester (Thermo Fisher Scientific, A20000 or A20006) to lysine side chains in PBS and molar ratio of dye to Fab was controlled to be in the range of 1 ∼ 2.
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bioRxiv - Neuroscience 2022Quote: ... acutely dissociated DRG neurons from 4 mice were first incubated in at 37 °C for 30 minutes in 4 mm Fura-2-acetoxymethyl ester and 0.2% Pluronic® F127 (Thermo Fisher Scientific, Waltham, MA), washed three times ...
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bioRxiv - Molecular Biology 2023Quote: ... The amino-modified forward primers (synthesized by IDT) were chloroform extracted twice before labeling with Alexa647- or Alexa555-conjugated ester according to the manufacturer protocols (Thermo Fisher, A20106 and A20109). The labeled primers were purified by PAGE and were used in combination with reverse primer EL338 or EL873 to amplify eBlock gene fragments (IDT) ...
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bioRxiv - Cancer Biology 2023Quote: ... Next day the cells were washed with fresh media and incubated at 37°C for 45 min in culture media containing 4 μM Fura-2/acetoxymethyl ester (Molecular Probes, Eugene, OR, USA). Then the cells were washed and kept in HEPES-buffered saline solution (140 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant VP1u proteins were purified using Ni-NTA beads and B19-VP1u molecules were crosslinked to fluorescently labelled MS2-VLPs or PP7 using a maleimide–NHS–ester bifunction linker (22114, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: ... 0.1 mM non-essential amino acids (Thermo Scientific,1140-050), and were incubated at 37C° and 5% CO2 in a humidified incubator ...
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bioRxiv - Biophysics 2021Quote: ... 1x non-essential amino acids and mercaptoenthanol were from Gibco, Life Technologies (USA) ...
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bioRxiv - Biophysics 2020Quote: ... The resolved nucleic acids were stained with SYBR-GOLD (Invitrogen) and gel images were obtained using a Chemidoc-MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted by Acid phenol :CHCL3 (Thermo Fisher Scientific), and treated with Nuclease P1 (New England BioLab ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted by Acid phenol :CHCL3 (Thermo Fisher Scientific). Pri-miRNA levels were analyzed by qRT-PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted by Acid phenol :CHCL3 (Thermo Fisher Scientific), and run on 15% TBE-Urea PAGE ...
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bioRxiv - Cell Biology 2020Quote: ... 5.6 mM D-Glucose and MEM amino acids solution (ThermoFisher 11130051 ...
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bioRxiv - Cell Biology 2020Quote: ... 1x non-essential amino acids (NEAA; ThermoFisher Scientific 11140-050); 1 mM sodium pyruvate (ThermoFisher Scientific 11360070) ...
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bioRxiv - Cell Biology 2020Quote: ... 1x MEM non-essential amino acids (Gibco, Thermo Fisher, USA), 2 mM L-Glutamin (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... 1x MEM non-essential amino acids (Gibco, Thermo Fisher, USA), 2 mM L-Glutamin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Sodium Pyruvate and non- essential amino acids (Thermo Fisher 11140035). Cells were routinely checked for Mycoplasma Spp ...
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bioRxiv - Biochemistry 2020Quote: ... and hydrochloric acid (HCl) (technical grade) were from Thermo Fisher Scientific (Chicago ...
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bioRxiv - Molecular Biology 2021Quote: ... 50% glacial acetic acid (Fisher Scientific, A38-212, lot 172788)) on a coverslip for one minute ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1X MEM Non Essential Amino Acids (Gibco, Cat. No. 11140050), 1X Glutamax ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.1 mM MEM non-essential amino acids (Thermo Fisher Scientific), 1 mM sodium pyruvate (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... Add 300 μL Acid Phenol Chloroform (Thermo Fisher cat. AM9722). Note the total volume should be around 550 uL.
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bioRxiv - Genomics 2020Quote: Add 300 uL of Acid Chloroform (Thermo Fisher cat. AM9722).
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bioRxiv - Neuroscience 2022Quote: ... in the presence of Pluronic Acid F-127 (Molecular Probes) for 30 min at room temperature (RT) ...
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bioRxiv - Molecular Biology 2020Quote: A bicinchoninic acid (BCA) reagent kit (Fisher Scientific, Cat. #10249133), with a bovine serum albumin (BSA ...