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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 3% TFA (Thermo Scientific, 28903)) ...
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bioRxiv - Immunology 2023Quote: ... or TO-PRO-3 (ThermoFisher). Samples were mounted using ProLong Diamond Antifade Mountant and curated for 24 hours before imaging ...
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bioRxiv - Microbiology 2023Quote: ... 3 µL 0.1% BSA (Ambion), 3 µL 10x reaction buffer (NEB) ...
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bioRxiv - Immunology 2023Quote: ... 3 mM L-glutamine (Invitrogen), non-essential amino acids (NEAA ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 mM MgCl2 (Thermofisher: AM9530G); and 0.1% NP-40 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... for Qubit 3 fluorometer (Invitrogen) and 100 / 30 ng (immunoprecipitation/input ...
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bioRxiv - Biochemistry 2024Quote: ... DiIC12(3) (Thermo Fisher Scientific), was added to a final concentration of 5 μg/mL in GPMV buffer and incubated for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Step 3: Cavicid (Fisher Scientific), Step 4 ...
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bioRxiv - Cell Biology 2024Quote: ... Flt-3 (A2F10, Thermofisher Scientific), CD48 (HM48-1 ...
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bioRxiv - Neuroscience 2024Quote: ... pHrodo (Invitrogen, P36600, 3 μM) dissolved in DMSO was added to the synaptosomes and incubated for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml Puromycin (Gibco) was added 48 hours after transduction before knockdown analysed >8 days post transduction via qPCR and western blot if appropriate32.
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bioRxiv - Microbiology 2024Quote: ... 3 µg/ml puromycin (Gibco) was added 48 hours post-transduction ...
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bioRxiv - Microbiology 2024Quote: ... 3 μl Lipofectamine RNAiMAX (Invitrogen) was mixed with 100 μl Opti-MEM and incubated at room temperature for 1 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 3% bovine serum albumin (ThermoFisher) and 5% normal goat serum (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 3 µm column (Thermo Scientific) and a 6210 electrochemical cell (ESA ...
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bioRxiv - Cell Biology 2024Quote: ... with Quantstudio 3 (Applied biosystems). microRNA levels were normalized by as the combination of endogenous control as internal standard ...
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bioRxiv - Cancer Biology 2024Quote: ... and QuantStudio 3 (Thermo Fisher). All primers used here are listed in Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: Splenocytes isolated three weeks after the second immunization were labeled with 0.5 μM carboxyfluorescein-succinimidyl-ester (CFSE) (ebioscience, Life Technologies, Carlsbad, CA, USA) as previously described (49) ...
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Systems Biology 2021Quote: The relative mitochondrial transmembrane potential (ΔΨm) was measured using the membrane-potential-dependent fluorescent dye TMRE (Tetramethylrhodamine, Ethyl Ester, Perchlorate) (Molecular Probes, Thermo Fisher Scientific)[87] ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation was also analyzed using the carboxyfluorescein diacetate succinimidyl ester labeling method with the CellTrace™ CFSE Cell Proliferation Kit (Invitrogen, CA, USA) in cells synchronized by gradual serum deprivation following the protocol described by Lauand and collaborators 23 ...
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bioRxiv - Biophysics 2020Quote: ... then incubated with a mix 9:1 poly-L-lysine:Alexa Fluor 546-labelled poly-L-lysine solution (0.1% or 0.01% w/v H2O, Sigma P8920-100ml, labelled with Alexa Fluor 546 NHS Ester Life Technologies A20102 following provider protocols) for 45 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The washed fractions were then split into two equal volumes and stained with either Texas Red®-X succinimidyl ester (TRSE, Molecular Probes™) or AlexaFlour Green 488 succinimidyl ester (SE488 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Immunology 2022Quote: Allogeneic CD4 T cells were isolated from PBMC using a positive-selection T cell isolation kit and labeled with 5mM/ml carboxyfluorescein diacetate succinimidyl ester (CFSE) as per protocol (C34554, Molecular Probes, Life Technologies). Enriched pDC were exposed to LPS with or without HIVMN AT-2 stimulation in the presence of 10ng/ml Interleukin-3 (IL-3 ...
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bioRxiv - Neuroscience 2022Quote: ... according to the manufacturer’s protocol and then labelled with pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE) (ThermoFisher Scientific; Cat. No. P36600) as previously described (Dräger et al. ...
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bioRxiv - Biophysics 2022Quote: ... The terminal free amine on the spacer was reacted with the N-hydroxysuccinimide (NHS) ester group of sulfo-SMCC (Thermo Fisher Scientific, 22622). The maleimide group of sulfo-SMCC was subsequently conjugated to 3’ thiol modified DNA oligonucleotides as described19 ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Microbiology 2024Quote: ... The organic phase was then removed by dialysis (24 h, room temperature) using a 100 kDa MW cut-off cellulose ester membrane (Thermo Fisher Scientific, France). Empty nanoformulation was prepared according to the same protocol but in absence of drug in the organic phase ...
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bioRxiv - Biochemistry 2023Quote: The RBD protein was incubated for 30 min at 21 °C and 750 rpm with a 10 molar equivalent of sulfo-m-maleimidobenzoyl-N-hydoxysuccinimide ester (Sulfo-MBS) (Thermo Scientific; MW 416.30). The excess crosslinker was removed by passing the protein through a PD-10 column using a PBS ...
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bioRxiv - Biophysics 2023Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Experiments were conducted as described in our previous studies.(36) Neurons were loaded with the fluorescent calcium indicator Fura-2-acetomethoxyl ester (2 μg/mL, Molecular Probes, Eugene, OR) for 45 mins in the dark at room temperature and then allowed to de-esterify for 15 min at 37 °C in the warm external solution ...
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bioRxiv - Biochemistry 2024Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... aliquots of aerobic or microaerobic cells were incubated with 200 nM of membrane potential sensitive dye tetramethylrhodamine ethyl ester (TMRE, Thermo Fisher Scientific T669). Cells were first incubated in the dark with dye for 20 minutes at room temperature followed by three washes in water prior to imaging with a 561 nm laser line ...
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bioRxiv - Genomics 2024Quote: ... was prepared by dissolving 183.16 mg of NHS ester in 1000 μL of anhydrous dimethyl sulfoxide (DMSO) (Thermo Fisher Scientific, catalog number 276855). Aliquots were stored at -20°C in a falcon tube with desiccant (Drierite # 23001 ...
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bioRxiv - Biochemistry 2024Quote: ... F-actin was incubated for 2 h at room temperature with a 5-fold molar excess of Alexa-488 NHS ester dye (Thermo Fisher Scientific, USA). F-actin was then pelleted by centrifugation at 450,000 × g for 40 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... while the rest were re-plated onto three matrigel-coated 10 cm dishes (3 replicates / 3 million cells per plate) in N2/B27 media containing 3 µg/mL puromycin (Fisher Scientific; Cat. no. 501532829) to select for the pMK1334 sgRNA vector ...
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bioRxiv - Genomics 2020Quote: ... and total nucleic acids were extracted using MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit following the manufactures recommended conditions (Applied Biosystems; A36716)
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bioRxiv - Molecular Biology 2022Quote: ... LC mobile phase solvents consisted of 0.1% formic acid in water (Buffer A) and 0.1% formic acid in 80% acetonitrile (Buffer B, Optima™ LC/MS, Fisher Scientific, Pittsburgh, PA). After 22 uL of sample loading at a maximum column pressure of 700 bar ...
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bioRxiv - Neuroscience 2023Quote: ... organoids were fed with Forebrain Forth Media (Neurobasal Life Technologies 21103-049, Ascorbic Acid Sigma-Aldrich A4544, Glutamax Life Technologies 35050-061, MEM-Non Essential Amino Acids Gibco 11140050 ...
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bioRxiv - Cell Biology 2024Quote: ... We prepared matched amino acid-replete and amino acid-free media in the following way: Amino acid-free DMEM (US Biologicals, D9800-13) containing 10% dialyzed FBS (Life Technologies, A33820-01) served as our amino acid deprivation media and as our base media for making complete media ...
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bioRxiv - Microbiology 2024Quote: Total nucleic acid from feces from Cm-infected and control mice were extracted using a magnetic-based total nucleic acid isolation kit (MagMAX Total Nucleic Acid Isolation Kit, Life Technologies, Carlsbad, CA) following internally optimized protocols ...
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Neuroscience 2022Quote: RNA was 3’ labelled with biotin using Pierce™ RNA 3’ End Biotinylation Kit (ThermoFisher) and purified using Monarch® RNA Cleanup Kit (New England Biolabs).
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bioRxiv - Bioengineering 2024Quote: ... Red blood cells were lysed following 3 × 3 min incubations with ACK Lysing Buffer (Gibco). The remaining white blood cells were washed with PBS twice and plated for surface staining ...
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bioRxiv - Microbiology 2023Quote: ... Head kidney pieces (3 mm × 3 mm) were collected in RNA later (Thermo Fisher Scientific), transferred to 500 μL MagNA Pure LC RNA Isolation Tissue Lysis buffer ...