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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Gdf1/3 and Gdf1/3-like mRNA were synthesized using T7 mMESSAGE mMACHINE kit (Ambion). Unfertilized eggs were injected with mRNAs and fertilized as previously described50 ...
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bioRxiv - Bioengineering 2024Quote: ... Dil Stain (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (‘DiI’; DiIC18(3))) were purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were washed three times with Tris wash buffer and incubated for 1 hr at RT on a plate shaker with 25 μl of 2 μg/ml detection antibody (methyl Tau antibodies (INNOVAGEN) or BT2 (Thermo Fisher, cat. no. MN1010) both labeled with MSD Sulfo-Tag-NHS-Ester (Meso Scale Discovery ...
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bioRxiv - Genomics 2020Quote: ... We extracted DNA with 0.9 mL Extraction Buffer (1% SDS [Fisher Scientific], 240 mg/L para-aminosalicyclic acid [ACROS], 484 mg/L Tris/HCl [Fisher Scientific] ...
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bioRxiv - Molecular Biology 2021Quote: ... and plated onto Geltrex-coated dishes with MEF conditioned medium (CM) (DMEM/F12 supplemented with 15% KSR, 100μM β-mercaptoethanol, 1% nonessential amino acid stock (all from Thermo Fisher Scientific), 10ng/mL bFGF and 10 mM Y-27632 (both from the Institute for Technical Chemistry ...
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bioRxiv - Biochemistry 2020Quote: ... medium supplemented with 10% (v/v) foetal bovine serum (FBS, Thermo Fischer Scientific) and 1% (v/v) non-essential amino acids (NEAA, Thermo Fisher Scientific). Growth medium was refreshed on average every 48 h and cells were generally split 1/10 when at 60-70% confluence.
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bioRxiv - Evolutionary Biology 2021Quote: ... Bacterial DNA was stained using SYTO®41 Blue Fluorescent Nucleic Acid Stain (Molecular Probes #S11352, 1:200 vol/vol, wt/vol) in flow cytometry buffer (100 μL ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of extracted nucleic acid was added to 0.8 μL of SuperScript® III RT/Platinum® Taq Mix (Invitrogen, Australia), 10 μL of 2x Reaction Mix ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). A549 cells (human ...
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bioRxiv - Molecular Biology 2021Quote: ... were cultivated in minimum essential medium supplemented with 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). 293T cells that stably express ACE2 were generated by retroviral (murine leukemia virus ...
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bioRxiv - Plant Biology 2022Quote: ... The specificity of the primers was further validated using PCR and DNA gel electrophoresis on 1% (w/v) agarose gels containing 1X SYBR Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific) in 1X TAE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech), 1x non-essential amino acid solution (from 100x stock, PAA) and 1 mM sodium pyruvate (Thermo Fisher Scientific). Calu-3 (ACE2 ...
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bioRxiv - Molecular Biology 2020Quote: ... to 40% of solvent B (acetonitrile with 0.1% formic acid) for 100 min were applied on nanoACQUITY UPLC (Waters) coupled with Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific), which was operated at sensitive mode using the following parameters ...
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bioRxiv - Microbiology 2022Quote: ... bacterial were incubated in hot phenol lysis solution (1% SDS, 2 mM EDTA, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) for 45 minutes at 65 °C ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were lysed in a hot (60 °C) phenol solution (2 mM EDTA, pH8, 1% SDS, 40 mM sodium acetate in acid phenol (Invitrogen #15594-047)) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting fractions were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μg peptides were resuspended in 1% formic acid diluted in water and loaded on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). Settings used were as previously described [22] ...
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bioRxiv - Immunology 2020Quote: ... Bacterial DNA was stained using SYTO®41 Blue Fluorescent Nucleic Acid Stain (Molecular Probes #S11352, 1:200 vol/vol, wt/vol) or with SYBR® Green I Nucleic Acid Gel Stain (Invitrogen #S-7563 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were resuspended in 35μl of 1% formic acid and 15 μl were loaded onto a trap column (PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
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bioRxiv - Genetics 2020Quote: ... E14 mESCs were seeded at 1×106 cells per 10cm dish in mESC media without LIF + 10uM retinoic acid: Knockout DMEM (ThermoFisher Scientific, 10829018), 15% Embryomax FBS (MilliporeSigma ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were stained for DNA using either SYBR Green I Nucleic acid gel stain Ex504/Em523 (10,000 X concentrate in DMSO – subsequently diluted to 1:1000) (Invitrogen, Thermo Fisher, UK) or SYTO 9 Green Fluorescent Nucleic acid stain Ex485/Em498 ...
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bioRxiv - Microbiology 2020Quote: ... and the pellets were resuspended in 1 ml staining buffer supplemented with 0.5 μM Sytox Green Nucleic Acid Stain (Molecular Probes, Life Technologies), followed by incubation in the dark for 5 min ...
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bioRxiv - Immunology 2021Quote: ... peptides (1 μg) dissolved in 5% formic acid and analyzed by LC-MS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex ultra-high-pressure liquid chromatography system (RSLCnano).
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bioRxiv - Immunology 2022Quote: ... Samples were resuspended in 0.1% formic acid at approximately 1 µg worth of digest per µl in glass HPLC inserts (Thermo Fisher C4010-630) prior to analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (ThermoFisher Scientific, Inc).
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bioRxiv - Bioengineering 2022Quote: ... is purchased from MakingCosmetics Inc. (USA). 4- (2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1M solution, Cat. No. J16924-AP) is purchased from Thermo Scientific (USA). Silica beads (Cat ...
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bioRxiv - Physiology 2022Quote: ... were homogenized by ultrasonication (Q55 Sonicator, Qsonica Inc., USA) in 5 volumes of 1 M perchloric acid (PCA; A228 Fisher Scientific, USA). The samples were neutralized by adding KHCO3 ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... and 100 µg/ml streptomycin supplemented with 1X Non-Essential Amino Acid solution and 1 mM sodium pyruvate (Life Technologies, Gibco™). HEK293 cells were purchased from ATCC and maintained in DMEM containing 10% (v/v ...
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... and 100 µg/ml streptomycin supplemented with 1X Non-Essential Amino Acid solution and 1 mM sodium pyruvate (Life Technologies, Gibco™). HEK293 cells were purchased from ATCC and maintained in DMEM containing 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Codon-optimized gene encoding the full-length SARS-CoV-2 PLpro (1-315 amino acids) was custom synthesized (Invitrogen, Thermo Fisher Scientific) and was cloned using XhoI and NdeI restriction sites in pET28c vector containing an N-terminal 6X histidine tag ...
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bioRxiv - Microbiology 2023Quote: ... The enriched phosphopeptides were evaporated to dryness and then resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... were evaporated to dryness and resuspended in 1% formic acid prior to analysis by nano-LC MSMS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... cells were supplemented with either DMSO (PanReac AppliChem; #A3672) or different metabolites: formic acid (low dose 0.25 mM, high dose 1 mM, Thermo Fisher Scientific; #A11750), folate (low dose 0.125 μM ...
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bioRxiv - Microbiology 2023Quote: Digested peptides were resuspended in 0.1% formic acid and 40% of each specimen was analyzed on an LTQ Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) coupled with a Dionex Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific) ...
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Stability and heterogeneity in the anti-microbiota reactivity of human milk-derived Immunoglobulin AbioRxiv - Immunology 2023Quote: ... All the wells in the 96-well plate were then stained with 50μL secondary antibody staining mixture of Syto BC [(Green Fluorescent nuclear acid stain, Life Technologies (1:400)] ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The dried peptides were then reconstituted in 0.1% formic acid for analysis by a Q-Exactive Plus mass spectrometer coupled to an EASY-nLC 1200 system (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: ... Quantitative RT-PCR was performed in 1 μL of one-fifth diluted cDNA in 35-μL reactions containing the SYBR™ Green I Nucleic Acid Gel Stain (1:20,000 final dilution; Thermo Fisher Scientific), Taq DNA polymerase (1.75 units ...
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bioRxiv - Systems Biology 2024Quote: ... About 2 µg per fraction in 1% formic acid was analyzed in 90 min by LC-MS on an Orbitrap Ascend (Thermo Fisher Scientific) using a Real-Time-Search MS3 method25 ...
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bioRxiv - Cell Biology 2024Quote: ... using a 90 min gradient from 5-60% acetonitrile with 0.1% formic acid and directly sprayed via a nano-electrospray source in a quadrupole Orbitrap mass spectrometer (Q ExactiveTM, Thermo Fisher ScientificTM)76 ...
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bioRxiv - Neuroscience 2024Quote: ... mitotic SH-SY5Y cells were differentiated into non-mitotic neuronal cells by the addition of 20 µM all-trans retinoic acid on day 1 (Molecular Probes/Invitrogen, #207340010). From day 1 onwards ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR products were purified via gel electrophoresis using 1% agarose gels and products were identified using GelRed® Nucleic Acid Gel Stain (Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... Eluted peptides were dried overnight before being resuspended in 50 µl 1% formic acid and peptide concentration measured using the CBQCA assay following manufacturers protocol (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... or RTF1 amino acids 1-321 were codon-optimized for expression in yeast and purchased from Invitrogen (GeneArt; see Supplementary Table S4). Gibson assembly was used to subclone desired fragments into pGBT9 or pGAD424 (88) ...
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bioRxiv - Cell Biology 2024Quote: ... we used CEP135 immunofluorescent staining using a rabbit polyclonal antibody raised against the first 233 amino acids of CEP135 (24428-1-AP, Thermo Fisher Scientific). Sperm samples were visualized using a Leica SP8 confocal microscope in BrightR mode using an HC PL APO CS2 63x/1.40 OIL lens ...
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bioRxiv - Molecular Biology 2024Quote: ... the composition of the media remained the same as that used with aBMSCs or DPSCs except with the addition of non-essential amino acids (1%, Thermo Fisher Scientific) and reduced concentration of FBS to 10% ...
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bioRxiv - Bioengineering 2024Quote: ... through the Z-plane of the HeLa cells that were counterstained with SYTOX™ Deep Red Nucleic Acid Stain (1:2000, Invitrogen, #S11380). These images were deconvoluted using the default settings of Huygens Professional (v22.10 ...
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bioRxiv - Cell Biology 2022Quote: ... metaphase II-arrested eggs were microinjected with 2-3 picolitres of Rec8 antiserum (13) (1:2 dilution) and Alexa Fluor 488 Dextran 10,000 MW (Molecular Probes, D22910; 1:40 dilution) in 0.05% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... slides were then incubated simultaneously with two different secondary antibodies diluted in PBS/0.1% Tween 20/3% BSA for 1 h at RT to amplify the signal (goat anti-mouse Alexa Fluor 488 (Thermo Fisher, A11001; 1:250) and donkey anti-goat Alexa Fluor 488 (Thermo Fisher ...