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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for 6 CHLORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 × 105 cells were seeded in a 6-well plate (140675, Thermo Fisher Scientific) in which glass coverslips coated with collagen IV (Nitta Gelatin ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were rinsed with PBS and changed to Essential 6 (Thermo Fisher Scientific, A1516401) 24hrs after plating ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and exposed to Essential 6 (Thermo Fisher Scientific, A1516401) with 10µM SB431542 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... cells were supplemented with blasticidin S (6 µg mL-1, Thermo Fisher Scientific, A1113903). The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed on the QuantStudio 6 real-time PCR system (Applied Biosystems) using cDNA synthesised from 0.5 μg DNAse-treated RNA using the Superscript IV Reverse Transcriptase kit (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The denatured RNA samples were loaded into 6% polyacrylamide TBE-Urea gels (Invitrogen, USA), and electrophoresis was performed for 90 min in 1xTBE ...
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bioRxiv - Neuroscience 2023Quote: ... and RT-qPCR was performed on the QuantStudio Pro 6 system (Thermo Scientific, A43180) using PowerTrack SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... 6 pmol of DsiRNA were diluted in 100 µL Opti-MEM media (Gibco #31985062) and pipette-mixed ...
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bioRxiv - Bioengineering 2023Quote: GBM-43 and GBM-6 (Mayo Clinic) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% (vol/vol ...
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bioRxiv - Biophysics 2023Quote: ... Medium was changed 6 h after transfection with regular medium (11995-065; Gibco BRL).
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bioRxiv - Cell Biology 2023Quote: ... The media was changed after 2 days to Essential 6 (Invitrogen Cat. No. A1516401) supplemented with b-FGF 20ng/mL (Peprotech US ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... C57BL/6 mice were administered with 10 µL per nostril of free DiR (Thermofisher) or PCANS mixed with DiR at a final concentration of 10 µg/ml) ...
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bioRxiv - Immunology 2023Quote: ... Amplifications were performed with a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). The assay lower LOD was 50 copies per reaction.
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bioRxiv - Molecular Biology 2023Quote: ... Fragment data were analyzed and visualized using the GeneMapper software version 6 (Applied Biosystems), and processed further using Adobe Illustrator ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections were embedded in cool 6% low-melting point agarose (ThermoFisher, Waltham, MA) in DPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA nicks were sealed by addition of 6 µL T4 ligase buffer (Thermo Fisher) and 2 µL T4 ligase (10 U ...
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bioRxiv - Bioengineering 2023Quote: ... in tissue culture 6-well plates coated with reduced growth factor Geltrex (Gibco, A1413202) diluted in cold DMEM/F12 (dilution ...
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bioRxiv - Genomics 2023Quote: RNA from cells grown in 6 well plates was extracted using TRIZOL (Invitrogen 15596026) and reverse transcribed using Superscirpt II Reverse Transcriptase (Invitrogen 18064022 ...
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bioRxiv - Cancer Biology 2023Quote: ... CyQuant Direct reagent was added on day 6 according to the manufacturer’s protocol (ThermoFisher). Quantification was performed using a BioTek Synergy 2 plate reader.
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bioRxiv - Systems Biology 2023Quote: ... Reactions were performed in a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific), using SYBR Green chemistry (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Quantification was performed in triplicate using the QuantStudio 6 Pro qPCR machine (ThermoFisher Scientific). Rpl19 was used as the endogenous control for relative quantification.
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bioRxiv - Immunology 2023Quote: Amplifications were performed with a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). The assay lower LOD was 50 copies per reaction.
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bioRxiv - Cancer Biology 2024Quote: ... 1.25*105 cells/well were seeded in 6-well plates (Thermo Scientific cat# 140675) and for mitochondrial enrichment ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from duplicate wells of 6-well plates using TRIzol (Invitrogen) according to the manufacturer’s instructions and quantified using a BioAnalyzer (Agilent) ...
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bioRxiv - Bioengineering 2024Quote: ... HEK293T cells were grown in a 6-well culture plate (ThermoFisher, cat. no. 140675) in DMEM with 10 % FBS (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were run on a QuantStudio 6 Flex 384-well qPCR apparatus (Applied Biosystems). Each target gene was normalized to HPRT ...
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bioRxiv - Cancer Biology 2024Quote: ... MSCs were used up to passage 6 and harvested by incubating with TrypLE (Gibco) to gently detach the cells from the culture surface.
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA-infected cells cultured in 6-well plates were washed by DPBS (Gibco, #C14190500BT), followed by detachment using accutase (BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was suspended in urea Dye and resolved on a 6% TBU gel (Invitrogen) at 200 V for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed in a QuantStudio 6 Flex PCR System (Applied Biosystems) using the Fast SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485689). A table with qPCR primer design is included (Supplementary Table 7).
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bioRxiv - Neuroscience 2024Quote: HeLa cells were plated in DMEM (Cellgro, #10-013CV) with 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture-treated plates ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were first treated with 6× Laemmli SDS sample reducing dye (Thermo scientific) and boiled for 5 min at 100 °C ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 PD 34 to PD 40 were seeded on 6 well plates (ThermoFisher, 140675) as the beginning points for the assays ...
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bioRxiv - Cancer Biology 2024Quote: ... All qPCR was run on QuantStudio 6 flex real-time PCR system (Applied Biosystems). Relative abundance was calculated with delta-delta-Ct method ...
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bioRxiv - Cell Biology 2024Quote: ... C2C12 cells on 6-well plates were transiently transfected with RNAiMAX (Invitrogen, 13778-150) according to the manufacturer’s directions in two stages to prevent over-confluency at the time of cellular treatment and lysis ...
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bioRxiv - Genetics 2021Quote: ... and cytocentrifuged on clean slides (using a Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific, at 900 rpm for 4 min). Slides were then immersed in liquid nitrogen for 5 min ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Developmental Biology 2019Quote: Mouse tissues were fixed in 4% paraformaldehyde at 4°C overnight and embedded in paraffin or Sandon Cryomatrix (Thermo Scientific). Standard histology techniques were used ...
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bioRxiv - Physiology 2019Quote: ... 20-30 μ g of protein lysates were denatured in NuPAGE 4× LDS sample buffer and resolved on NuPage 4-12 % Bis-Tris gels (Invitrogen) and the proteins transferred by iBlot (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... for 20 min at 4 °C and were then centrifuged at 444g for 4 min and dissociated to single cells using StemPro Accutase (Invitrogen). Cells were subsequently fixed using 4% paraformaldehyde (PFA ...
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bioRxiv - Bioengineering 2020Quote: ... and media for M(IL4/IL13) stimulation consisted of RPMI 1640 with 20 ng/ml interleukin 4 (IL-4; Invitrogen) and 20 ng/ml interleukin 13 (IL-13 ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...