Labshake search
Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Coverslips were blocked for one hour at RT in buffer containing 5% normal goat serum (NGS) (ThermoFisher: PCN5000) and 0.3% Triton-X in 1x PBS and incubated in primary antibodies in antibody dilution buffer (1% BSA and 0.3% Triton-X in 1x PBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... One µg of total RNA was reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). cDNA was diluted at 1:10 in ddH2O and stored at -20°C ...
-
bioRxiv - Microbiology 2021Quote: ... the RNA was amplified using the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher). A known quantity of pNL4-3.Luc.R-E-vector was used to generate standard curves ...
-
bioRxiv - Microbiology 2021Quote: ... the RNA was amplified using the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher). A known quantity of pNL4-3.Luc.R-E-vector was used to generate standard curves ...
-
bioRxiv - Bioengineering 2021Quote: ... was used for qRT-PCR experiments on a Step-One Real-time PCR System (Thermo Fisher, Waltham, MA). Detailed steps are listed in Method S5.
-
bioRxiv - Biochemistry 2020Quote: ... One microgram of RNA was used for cDNA synthesis using the First Strand cDNA synthesis kit (Thermo Scientific). Purified RNA (100 ng ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were grown for one week in amplification medium (AM) containing high-glucose (4.5 g/l) Dulbecco’s modified Eagle medium (DMEM; Gibco) supplemented with 20% heat-inactivated fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2020Quote: ... the RNA was amplified using the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher). A known quantity of pNL4-3.Luc.R-E-vector was used to generate standard curves ...
-
bioRxiv - Microbiology 2020Quote: ... the RNA was amplified using the TaqMan One-Step RT-PCR Master Mix Reagents (Applied Biosystems, Thermo Fisher). A known quantity of pNL4-3.Luc.R-E-vector was used to generate standard curves ...
-
bioRxiv - Systems Biology 2020Quote: ... all coverslips were mounted on slides with one drop of ProLong Gold Antifade mountant (Thermo Fisher Scientific, P10144), dried in the dark overnight at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... Real-time RT-PCR was performed with the EXPRESSS One-Step SuperScript qRT-PCR kit (Thermo Fisher Scientific) using gene-specific primers (Supplementary Table 2 ...
-
bioRxiv - Immunology 2021Quote: ... CV9032B) or following WHO guidelines using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Thermo Fisher) and TaqMan probes against SARS-CoV-2 E- and RdRp (Eurofins Scientific) ...
-
bioRxiv - Physiology 2021Quote: ... One microgram of RNA was transcribed into cDNA using the high-capacity cDNA reverse transcription kits (Applied Biosystems). Quantitative RT-PCR analysis was performed on an ABI ViiA 7 Real-time PCR system (Applied Biosystems ...
-
bioRxiv - Plant Biology 2021Quote: ... Powdered tissue was transferred to a 1.5 ml centrifuge tube containing one ml of Trizol (Invitrogen, Carlsbad, CA). RNA was extracted using the Trizol Plus RNA Purification Kit (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... Approximately 3 × 106 HEK293F cells were transfected with one of these constructs using 293fectin Transfection Reagent (Thermo Fisher) or 293-Free (EMD Millipore) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and labeled with one TMT label (126, 127N, 127C, 128N; TMT 10-plex kit 90110 from Thermo Scientific), dissolved in DMSO in a 1:5 (total protein/ TMT label ...
-
bioRxiv - Pathology 2021Quote: ... The PCR mastermix was prepared using the AgPath-ID One-step RT-PCR kit (Thermo Fisher Scientific, USA) in a volume of 10 µl including 0.5 µl of β-Actin-mix2-HEX as internal control and 2.5 µl of extracted RNA ...
-
bioRxiv - Cell Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using the SuperScript II cDNA Synthesis Kit (Invitrogen, Eugene, OR). Quantitative Real-Time PCR (qRT-PCR ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were incubated for one hour with AlexaFluor®-conjugated donkey secondary antibodies (1:500, Thermo Fisher Scientific) and finally counterstained with Hoechst 33342 (20 μm ...
-
bioRxiv - Cancer Biology 2022Quote: One hundred thousand Raji-GPR183WT or Raji-GPR183KO cells were then stained with Hoechst 33342 blue dye (Invitrogen) and cultivated in a conditional medium with 25,000 StromaNKtert-GFP cells for 2 days to generate the BL 3D spheroids ...
-
bioRxiv - Cell Biology 2022Quote: ... Cell analysis was performed on either one of the flow cytometers (Attune NxT, Thermo Fisher; Cytek AURORA, Aurora).
-
bioRxiv - Neuroscience 2022Quote: ... The concentration and purity of the extracted total RNA were checked on a NanoDrop™ One (Thermo Scientific) and three samples for each experimental group were selected.
-
bioRxiv - Plant Biology 2022Quote: ... All the probes of one set were pooled and en masse coupled with Alexa Fluor 647 (Thermo Fisher) or Texas Red ...
-
bioRxiv - Molecular Biology 2022Quote: ... Protein samples (50 μg) were resolved by one-dimensional denaturing 12 % Novex Trisglycine gels (Invitrogen, Burlington, ON, Canada) and transferred onto polyvinylidene difluoride membranes (PVDF ...
-
bioRxiv - Molecular Biology 2022Quote: ... One μg of the RNA was reverse transcribed in a 10 μl reaction using SuperScript IV (ThermoFisher Scientific) and random primers (Agilent ...
-
bioRxiv - Neuroscience 2022Quote: ... qPCR reactions were performed in a Step One Plus cycler with Taqman Fast Advanced Master Mix (Applied Biosystems). SYBR green thermocycling was also performed in a final volume of 10 μl ...
-
bioRxiv - Neuroscience 2022Quote: ... One µg of RNA was retro-transcribed with the High-Capacity RNA-to-cDNA Kit (Thermo Fisher Scientific). qRT-PCR was carried out on an Abi 7900HT Sequencing Detection System (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... All the procedures follow the manufacturer’s instructions of the Applied Biosystems TaqMan Fast Virus one-step mastermix (ThermoFisher). Briefly ...
-
bioRxiv - Microbiology 2022Quote: Specific RT-PCRs using the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) were run to verify the presence of Clostridiaceae species (65) ...
-
bioRxiv - Microbiology 2022Quote: ... RT-PCR was conducted using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) to amplify the extracted viral RNA (Qiagen) ...
-
bioRxiv - Microbiology 2022Quote: One technical replicate from each sample was analyzed on a LTQ Orbitrap XL instrument (Thermo Scientific, Waltham, MA). It was coupled to an Agilent 1100 series LC (Agilent Technologies ...
-
bioRxiv - Biophysics 2022Quote: ... The protein concentrations were determined from the absorbance at 280 nm measured using NanoDrop One (Thermo Fisher Scientific) with the following absorption coefficients ...
-
bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was retrotranscribed to complementary DNA using moloney murine leukemia virus reverse transcriptase (Thermo Fisher) and random hexamer primers (Roche) ...
-
bioRxiv - Plant Biology 2021Quote: ... The DNA quantity and quality were determined using agarose gel electrophoresis and NanoDrop One spectrophotometer (Thermo Fisher Scientific). The genotyping was performed using CAPS markers that discriminate Solanum lycopersicum cv ...
-
bioRxiv - Microbiology 2021Quote: ... Amplifications were performed by qPCR using SuperScript III Platinum One-Step Quantitative RT-PCR System with ROX (Invitrogen) according to the manufacturer’s instructions in the presence of primers and probes described previously (Goncalves Pereira et al. ...
-
bioRxiv - Microbiology 2020Quote: ... The spike protein gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (ThermoFisher Scientific) using primers flanking the S gene ...
-
bioRxiv - Microbiology 2020Quote: ... reverse transcription PCR was performed on extracted RNA using SuperScript™ IV One-step RT-PCR System (Invitrogen). The sequence-specific primers were used to generate an amplicon of 905 bp covering the D614G region ...
-
bioRxiv - Microbiology 2020Quote: ... NM_001135099) were amplified using the SuperScript® III One-Step RT-PCR System with Platinum® Taq (Invitrogen) from 500 ng RNA obtained from 293T cells and Caco-2 cells ...
-
bioRxiv - Microbiology 2020Quote: SAV3 RNA from heart tissue was quantified with a modified one-step nsP-1 assay (Ag-Path, Ambion) (Jiraporn Jarungsriapisit et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... Experiments by triplicate were carried out in a Step One Real-Time-PCR instrument (Applied Biosystems, CA, U.S.A.).
-
bioRxiv - Immunology 2022Quote: ... The entire sample was used as template in a one-step RT-PCR reaction (Thermo Scientific, AB-4104A). After cDNA synthesis and PCR ...
-
bioRxiv - Immunology 2022Quote: ... and analyzed by RT-PCR with a Step One Real-time PCR system as described above (Applied Biosystems).
-
bioRxiv - Microbiology 2022Quote: ... One microgram of RNA was used to synthesize the cDNA with the Maxima Reverse Transcriptase kit (Thermo Scientific). qRT-PCR was performed with the CFX96 real-time PCR system (Bio-Rad) ...
-
bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Invitrogen) as per manufacturer’s instructions on a Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad) ...
-
bioRxiv - Pathology 2022Quote: ... Samples were then washed one time in PBS-Triton then incubated in secondary antibody solution (Invitrogen A-21200) with 10 µg.mL-1 antibody ...
-
bioRxiv - Microbiology 2019Quote: ... Quantitative RT-PCR was then performed with TaqMan RNA-to-Ct One-step RT-PCR kit (Applied Biosystems) using the following cycling conditions ...
-
bioRxiv - Biochemistry 2019Quote: ... Transcription levels of differently treated cells were analyzed by quantitative PCR using ABI Step One Plus (Applied Biosystems) according to the manufacturer’s protocol ...
-
bioRxiv - Systems Biology 2019Quote: ... succinate and malate were quantified with either one of two HPLCs: 1) a Dionex Ultimate 3000 (Thermo Scientific) containing an LPG-3400SD pump ...
-
bioRxiv - Genomics 2019Quote: ... Pooled extractions from one chosen individual yielded >100 ug genomic DNA as assessed by PicoGreen DNA quantification (ThermoFisher). The Agilent TapeStation Genomic DNA assay indicated that the majority of gDNA extracted was well over 20kb (not shown) ...