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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Neuroscience 2022Quote: ... and the samples were first postfixed overnight in 4 % PFA (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 d for decalcification ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then denatured by incubating samples at 95°C for 5 min in 4 x Laemmli buffer and dithiothreitol (DTT) before loading onto 4 to 12% NuPAGE gradient precast gels (ThermoFisher). Gels were run for 10 min at 110 V ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Microbiology 2024Quote: ... at physiological pH before being spun down and resuspended in PBS with the addition of FM 1-43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide (Cat. no. T3163, Invitrogen). Images were taken on a Zeiss X10 light microscope and cell area was measured using CellProfiler 4.2.1 (33,34).
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated overnight in 30% sucrose in PBS buffer after overnighted fixation at 4°C in 4% paraformaldehyde in PBS buffer (Invitrogen) until the intestinal tissues completely sink to the tube bottom ...
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bioRxiv - Neuroscience 2023Quote: Cells were fixed with 4% paraformaldehyde and stored at 4°C until blocked with 10% donkey serum in Phosphate-Buffered Saline (PBS) (Invitrogen) and 0.25% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Genomics 2023Quote: ... Samples were normalized by BCA quantifications and then loaded in 4 12% or 4-20% Novex Tris-glycine gels (Invitrogen) and run at 165V for ∼1 h in Tris-glycine buffer (25 mM Tris and 192 mM glycine ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed three times for 10 min in 1X PBS and incubated for 4 h at 4°C with the fluorescently tagged secondary antibodies (Invitrogen; AlexaFluor 647 goat anti-mouse and AlexaFluor 488 donkey anti-rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: Spleen tissues were fixed in 4% formaldehyde overnight at 4°C and then cut with a microtome (Micron KS34 ThermoFisher) to obtain 30 μm tissue sections86 that were then stained for 10 min with 0.3 µM DAPI in DPBS (Thermo Scientific™ #62247) ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were also stained for 30 minutes simultaneously with 1 μM 2′-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]−2,5′-bi-1H-benzimidazoletrihydrochloride trihydrate (Hoechst 33342, Fisher Scientific) for nuclear visualization and cell localization ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... 3% dilution, 4 µl,), Fluoro-ruby (FR, case BC, (10% solution, mixture of 10 kDA and 3 kDA, 4 µl; Invitrogen), and Biotinylated Dextran Amine (BDA ...
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bioRxiv - Molecular Biology 2024Quote: The redox state of Trx3 was measured by covalent modification with the thiol-reactive probe 4-acetamido-4’maleimidyldystilbene-2,2’-disulfonic acid (AMS; Molecular Probes) as described previously 49 ...
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bioRxiv - Plant Biology 2020Quote: ... The purity and concentration of the extracted RNA were determined with a NanoDrop One Spectrophotometer (Thermo Scientific, USA) and a precise concentration measurement with the Qubit 3 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... PB1-F2 (75P) cDNA was amplified via one step RT-PCR using SuperScript III/Platinum Taq mix (Invitrogen). The RT-PCR product was cloned into pCAGGS mammalian expression vector via restriction enzymes KpnI and SphI ...
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bioRxiv - Plant Biology 2020Quote: ... One hundred milligrams of each pulverized sample was used for extraction of total RNA using Trizol (Life Technologies), following the supplier’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... Purified LdGSTu1 protein concentrations were calculated using the Bradford assay or UV280 methods on NanoDrop One (Thermo Scientific) and Spark multi-mode plate reader (Tecan) ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently used for qPCR analyses on a Step One Plus real time PCR detection system (Applied Biosystems). Expression levels of genes of interest were normalized to hypoxanthine guanine phosphoribosyl transferase (Hprt ...
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bioRxiv - Cell Biology 2020Quote: ... plate area 34mm2) were seeded on Greiner Bio-One CELL view Cell Culture Slides (Fisher Scientific, Pittsburgh, PA) and allowed to adhere overnight ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies) using the TaqMan 2× Universal Master Mix and the following TaqMan Gene Expression Assays ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems, Life Technologies) using the TaqMan 2× Universal Master Mix and the following TaqMan Gene Expression Assays ...
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bioRxiv - Genetics 2021Quote: ... Real-time quantitative PCR was then performed on resulting cDNAs in a Step-One Plus system (Applied Biosystems) using PerfeCTa SYBR Green FastMix ROX (Quanta BioSciences) ...
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bioRxiv - Genetics 2021Quote: ... and Tvrm323 mice (n = 5) at one month of age by homogenizing posterior eyecups in TRIzol (Thermo Fisher) using a gentleMACS dissociator (Milteny Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... Q-PCR amplifications were performed on a Applied Biosystems Step One Plus RT PCR system (Thermo Fisher Scientific) at 95 °C for 10min ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR reactions were performed on a Step One Plus Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the THUNDERBIRD qPCR Mix (QPS-201 ...
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bioRxiv - Cancer Biology 2022Quote: ... qPCR reactions were performed on a Step One Plus Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the THUNDERBIRD qPCR Mix (QPS-201 ...
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bioRxiv - Cell Biology 2019Quote: ... One μg of total RNA was used for cDNA synthesis using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) and random hexamers (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... The cells were then images with one of two fluorescent microscopes: Life Technologies EVOS FL (Thermo Fisher Scientific) or Nikon Eclipse 90i microscope (Nikon Instruments Inc. ...
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bioRxiv - Plant Biology 2019Quote: ... Grids were clipped into Autogrid support rings modified with a cut-out on one side (FEI Thermo Fisher). In the preparation chamber ...
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bioRxiv - Cell Biology 2019Quote: ... One million cells were suspended in 5 milliliters of tenogenic growth media comprising high glucose DMEM (Thermo Scientific), 10% Cellect™ Silver fetal bovine serum (MP Biomedicals) ...
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bioRxiv - Microbiology 2019Quote: ... qRT-PCR was performed with the SuperScript III Platinum Syber Green One-Step qRT-PCR Kit (ThermoFisher Scientific) using 25 ng of DNased RNA following the manufacturer’s instructions on a BioRad iQ5 cycler with melt curve analysis.
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bioRxiv - Molecular Biology 2019Quote: Antibody-bead conjugates were prepared as follows: One hundred μL of protein G Dyna beads (Thermo Fisher Scientific) were washed three times in PLB and were resuspended in 1 mL of PLB ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were imaged one day after transfection: coverslips were mounted in an Attofluor cell chamber (Invitrogen, Breda, NL) and submerged in 1 mL microscopy medium (20 mM HEPES ...
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bioRxiv - Developmental Biology 2019Quote: ... Each single cell was collected in 5µl of 2X Reaction Mix (Invitrogen, CellsDirect One-Step qRT-PCR Kit) and stored at −80°C or processed immediately.
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bioRxiv - Molecular Biology 2019Quote: Samples were imaged one day after transfection: coverslips were mounted in an Attofluor cell chamber (Invitrogen, Breda, NL) and submerged in 1 mL microscopy medium (20 mM HEPES ...
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bioRxiv - Developmental Biology 2019Quote: ... coated ibidi plates and cultured for one week in high glucose Iscove’s Modified Dulbecco’s media (Gibco, Life Technologily) supplemented with 20%FBS ...
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bioRxiv - Microbiology 2019Quote: One milliliter of monomeric capsid (5 mg/ml) was dialyzed in SnakeSkin dialysis tubing 10K MWCO (Thermo Scientific) using a buffer that is high in salt and contains a reducing agent (buffer 1 ...
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bioRxiv - Genetics 2019Quote: ... Membranes were blocked for one hour at room temperature in SuperBlock T20 (TBS) Blocking Buffer (Thermo Fisher Scientific) and incubated with primary antibodies for one hour at room temperature or overnight at 4°C in blocking buffer with shaking ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by one rinse in water to removed salts prior to mounting with ProLong Diamond Antifade Mountant (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and wells were washed with PBS-T and one-step 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (Thermo Fisher Scientific) was added and quenched with 2M H2S04 before reading at 450 nm (HRP) ...
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bioRxiv - Neuroscience 2020Quote: ... One block from each donor was processed for cryosectioning and fluorescent Nissl staining (Neurotrace 500/525, ThermoFisher Scientific). Stained sections were screened for histological hallmarks of primary motor cortex ...
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bioRxiv - Physiology 2021Quote: ... One microgram of RNA was transcribed into cDNA using the high-capacity cDNA reverse transcription kits (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using a TaqMan One-Step RT-PCR Master Mix Reagents Kit (ThermoFisher, cat. # 4309169) and primers for a genomic locus (b-actin ...
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bioRxiv - Cancer Biology 2021Quote: ... Real-time PCR was carried out using an ABI Step One plus Real-Time PCR System (Applied Biosystems). Reactions for each sample were performed in triplicate ...
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bioRxiv - Microbiology 2021Quote: ... The RNAs were amplified using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...