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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The redox homeostasis of promastigotes was monitored both qualitatively and quantitively by 2-7 dichlorodihydro fluorescein diacetate (DCFDA) (Life Technologies, USA) staining ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Microbiology 2021Quote: ... CAS number: 328–42–7), 2-Ketoglutaric acid, disodium salt, dehydrate (>99%, CAS number: 305–72–6) were purchased from Acros Organics, Belgium ...
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Cancer Biology 2023Quote: ... CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen; 1:1000) was used to measure apoptosis ...
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bioRxiv - Immunology 2020Quote: ... 1-2×106 keratinocytes were mixed 1:1 with autologous fibroblasts in 400µl MEM (Gibco; 11380037) containing 1% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and the GFP reporter vector at ratios of 2:1:1:1 using Lipofectamine 2000 (Invitrogen). Forty-eight hours after transfection ...
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bioRxiv - Neuroscience 2020Quote: ... The sodium-sensitive ratiometric dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Schwerte, Germany) was used for detection of Na+ ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Cell Biology 2024Quote: ... The Triton-X permeabilization solution was removed and cells were washed 3×2 minutes with DPBS at RT before a blocking solution (1% BSA (Fisher Scientific, BP1600-100) was added to cells for one hour at RT with rocking ...
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bioRxiv - Immunology 2021Quote: ... and then blocked for 2 h with PBS-Tween (PBST) containing 2% (v/v) goat serum (Gibco, MD, USA). Three-fold serial dilutions of serum (starting at 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... was cultured at 2-8×105 cells/ml in Fischer’s medium with 20% horse serum and 2 mM L-glutamine (all Gibco).
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bioRxiv - Molecular Biology 2020Quote: ... proteins were dialysed in PBS for 2 x 2 hours using Slide-A-Lyzer™ Dialysis Cassettes (Thermo Fisher). Protein aliquots were frozen and stored at −80°C until use.
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bioRxiv - Immunology 2022Quote: Purified IL-2 expanded MAIT cells (MAITIL-2) were stimulated for 18 hours with anti-CD3/CD28 Dynabeads (Thermofisher), IL-12 (50ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Fura-2-acetoxymethyl ester (Fura-2-AM) and Pluronic F-127 were purchased from Molecular Probes (Eugene, OR, USA) and dissolved in dimethylsulfoxide (DMSO) ...
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bioRxiv - Cell Biology 2021Quote: 2 mg of 0-2 hr embryos were harvested and RNA was extracted with TRIzol Reagent (#15596026, ThermoFisher Scientific). The PAT test was conducted following the manufacturer’s protocol (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... SALK_015201 and irr-2 genomic DNA using gene specific primers (Supplemental Table 2) with Phusion polymerase (Thermo Fisher Scientific) and cleaned up using the Gene-JET PCR Purification Kit from Thermofisher (catalog number K0701) ...
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bioRxiv - Microbiology 2021Quote: ... 3×105 HEK293T cells were transfected with a 2:1:2 mass ratio of psPAX.2:pMD2.G:pLentiCMV/TO PuroDEST pUL21 (815 ng total DNA) using Lipofectamine 2000 (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... embryos were collected and brains were removed in ice-cooled Ca+2/Mg+2-free Hank’s balanced salt solution (Gibco) with 10mM HEPES (Thermofisher) ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Immunology 2022Quote: ... GATA-2-expressing vector was constructed by cloning synthesized mouse GATA-2 sequences into eukaryotic expression vector pcDNA3.1 (+) (Invitrogen) with restriction enzymes of NheI and EcoRI (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... both somal and axonal compartments were incubated with 2 μM of the calcium indicator dye Fura-2-AM (Invitrogen) in imaging buffer consisting of HBSS [Ca2+ and Mg2+-free] supplemented with 10 mM HEPES (ThermoFischer) ...
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bioRxiv - Neuroscience 2021Quote: ... 8% SDS and 2% β-mercaptoethanol) were loaded onto 12% SDS-polyacrylamide gel and See-Plus 2 (Invitrogen, LC5925) was used as a molecular-weight marker ...
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bioRxiv - Microbiology 2021Quote: Vero cells were seeded at 1.0 × 104cells per well with Dulbecco’s modified Eagle’s medium (DMEM; Welgene) supplemented with 2% heat-inactivated fetal bovine serum (FBS) and 2% antibiotic-antimycotic solution (Gibco) in a black ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was washed twice with 2 ml Neurobasal supplemented with 2% B27 and 0.5 mM L-glutamine (all from Gibco), and dissociated by 8–10 bouts of trituration ...
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bioRxiv - Cell Biology 2021Quote: ... and 2+2 μg of plasmid DNA encoding the proteins were mixed in Opti-MEM™ (Thermo Fisher Scientific) and applied onto the cells in a single well ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million SK-N-BE(2)-FLUC cells were stained immediately before injection with CellTrace™ Far Red (Invitrogen) following the manufacturer’s instructions ...