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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Silica gel was prepared by adding 2 g silica particles to 40 ml aqueous NaOH (0.01N; CAS 1310-73-2; Fisher Scientific) solution and magnetically stirring the mixture at a speed of 1000 rpm for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.4) containing 2 mM glucose for 2 hr with 100 nM of tetramethylrhodamine methyl ester (TMRM; Invitrogen, CA, USA). After the incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 μL (200 pmol) each of gRNA and tracRNA were combined with 2 μL of 5X annealing buffer (ThermoFisher 100061876) and 4 μL nuclease-free dH2O ...
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bioRxiv - Microbiology 2021Quote: ... coated with 200 ng/well ACE2 [produced in-house as described in Bosnjak et al.(25)] and blocked with 2% bovine serum albumin/2% mouse serum (Invitrogen) and incubated for additional 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of hK14-LaNto31-T2A-mCherry or LaNt-α31-pSec-Tag and 2 μl Lipofectamine 2000 (Thermo Fisher Scientific) were used ...
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bioRxiv - Bioengineering 2022Quote: ... Porcine DFAT adipocytes were stained for lipids via a 15-30 minute incubation at 37°C in lipid accumulation media (sans Intralipid) supplemented with 2 μM BODIPY 493/503 and 2 μg/ml Hoescht 33342 (H3570; Invitrogen). DFAT cells were then fixed in 4% PFA at RT for 10-15 minutes ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1% Glutamax (35050-038)) which was exchanged for 2 ml feeding medium 2 hours after plating (Neurobasal medium (21103-049, Gibco), 2% B27 (17504-044 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting peptic peptides were collected for three minutes on a trap column (2 mm x 2 cm) kept at 0.5 °C and filled with POROS 20 R2 material (Thermo Scientific). The trap column was then placed in line with an ACQUITY UPLC BEH C18 1.7 μm 1.0 x 100 mm column (Waters ...
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Trade-offs between antiseptic cytotoxicity and efficacy in a human ex vivo wound contamination modelbioRxiv - Microbiology 2021Quote: ... each bisect was rinsed in PBS and placed in an individual well of a 6-well plate with 2 mL MTT solution (2 mg/mL, Invitrogen) in each well ...
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bioRxiv - Neuroscience 2022Quote: ... The following three days (day 0-2) medium was replaced daily with 2 ml/well fresh differentiation medium: DMEM/F12/N2/NEAA (Invitrogen), penicillin/streptomycin (1% ...
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bioRxiv - Developmental Biology 2022Quote: ... the cultures were fed every 2 days with RPMI containing 2% B27 supplement plus insulin (Life Technologies Australia, Cat.#17504001). Cells were dissociated on day 15 of differentiation using 0.5% EDTA ...
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bioRxiv - Biophysics 2022Quote: ... at a concentration of 2×105 cells/ml (2 mL per well hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% (v/v) FBS (Gibco), glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in 2% donkey serum solution containing secondary antibodies conjugated to anti-rabbit Alexa488 (Invitrogen, Massachusetts ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed 3 times with 1x PBS and incubated for 1 hour at RT in the following secondary antibodies in 1x PBS containing 2% Triton X-100 and 2% horse serum: Goat anti-rabbit Alexa Fluor 488 or 594 (Invitrogen), Goat anti-mouse Alexa Fluor 594 or 647 (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Genomics 2021Quote: ... NGS was performed using primer sets that tiled the entire SARS-CoV-2 genome (Ion AmpliSeq SARS-CoV-2 Research Panel, ThermoFisher), with library preparation and sequencing performed on Ion Chef and S5 ...
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bioRxiv - Biochemistry 2022Quote: ... and recombinant human Interleukin-2 (rhIL-2, 100 units/mL)) in the presence of anti-CD3/CD28 Dynabeads (Fisher Scientific) at a bead:cell ratio of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... Slides were dehydrated by sequential submersion in 100% ethanol (2× 5 min) and SafeClear II (2× 5 min, Fisher Scientific) before being mounted in Cytoseal 60 (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 µl were applied to a freshly glow-discharged Quantifoil R 2/2 Cu/Rh 200 mesh grid and plunge-frozen into liquid ethane using a Vitrobot Mark IV (ThermoFisher) at 100% humidity at 10 °C ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 µl of the sample was applied to a freshly glow-discharged Quantifoil R 2/2 Cu/Rh 200 mesh grid and plunge-frozen into liquid ethane using a Vitrobot Mark IV (ThermoFisher) at 100% humidity at an ambient temperature of 10 °C ...
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bioRxiv - Microbiology 2022Quote: ... Vero E6 cells were infected with the SARS-CoV-2 wild type (isolate SARS-CoV-2/human/SWE/01/2020; Genbank accession: MT093571) in VP-SFM (Gibco). The virus was harvested at day three ...
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bioRxiv - Neuroscience 2023Quote: ... the plating medium was removed and replaced with maintenance media (neurobasal medium [Gibco, cat# 21103049] with 2% B27 [Gibco, cat# 17504044], 2 mM GlutaMAX [Gibco, cat# 35050061] ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from the thalamus of 2 GAERS (10 and 90 days) and 2 NEC (10 and 90 days) male rats using a MagMax Kit (Ambion) and full length cDNAs generated using SuperScriptII with oligo-dT priming (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... fraction in MACS buffer (2% heat-inactivated FBS, 2% 0,1M EDTA in PBSO) using the CD8+ Dynabead isolation kit (Thermo Fisher) and BD IMag Cell Separation Magnet (BD Biosciences) ...
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bioRxiv - Neuroscience 2023Quote: ... the DMEM+ was replaced with 0.5 mL of neurobasal medium (NBM: 2% B27, Invitrogen; penicillin/streptomycin; 2 mM α-glutamine; in neurobasal medium A, GIBCO). An additional 0.5 mL/well of NBM was added at 3 days in vitro (DIV) ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB-231 cells were plated as above and treated with 100 nM of Cy5-labeled si<(EG18L)2 or si<(EG18Ldiacid)2 in Opti-MEM (Gibco) for 2 h at 37°C vs ...
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bioRxiv - Synthetic Biology 2023Quote: ... in Tris-Glycine SDS Running Buffer (225V, 45 min)and transferred to nitrocellulose (iBlot 2, iBlot 2 NC Mini Stacks; Invitrogen) at 20 V for 1 min ...
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bioRxiv - Cell Biology 2023Quote: ... In brief pulse labeling with FM4-64 (2 μM FM464, Molecular Probes; made from a 2-mM stock in DMSO) was done for 5 min (time 0 ...
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bioRxiv - Pathology 2023Quote: ... Peptides were eluted under a 60min 2%-37% acetonitrile gradient for mouse degradome (2%-45% for human degradome) and transferred by Nanospray Flex nanoelectrospray source (ThermoFisher) into an Orbitrap Elite - ETD mass spectrometer (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... with IVT mix (0.5 µL Ribolock inhibitor, Invitrogen, 2 µL T7 polymerase buffer, NEB, 8 µL rNTP mix, 2 µL T7 polymerase, Invitrogen). DNA was removed by addition of 1 µL turbo DNase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...
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bioRxiv - Immunology 2023Quote: ... then incubated in wash buffer (Advanced DMEM, 10% FBS, 2 mM L-glutamate, penicillin-streptomycin) with 2 mg/mL collagenase type I (Invitrogen) at 37°C for 5-15 mins with vigorous mixing as previously described.57 Crypts were plated in Matrigel with Human Organoid Growth Media (StemCell) ...
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bioRxiv - Bioengineering 2023Quote: ... Cetuximab and Avelumab F(ab’)2 fragments were generated using the Pierce F(ab’)2 preparation kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... FT352-2 and MLA18-2 without stop codons and insect-cell codon-optimized Sr50 were obtained by gene synthesis (GeneArt, Invitrogen). Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cell populations were imaged in media at the designated time points at 10x magnification and 2×2 binning using the ArrayScanTM XTI High Content Reader (ThermoFisher). Tomato MFI and EGFP MFI was imaged with an excitation source of 560/25nm and 485/20nm respectively ...
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bioRxiv - Neuroscience 2023Quote: ... the pellet was washed with HBSS and then resuspended in 10 mL magnetic-activated cell sorting (MACS) buffer (Hibernate-A with 2 mM sodium pyruvate, 2% B27, 0.5% BSA; ThermoFisher Scientific), 2 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: Monolayers of HEK293T cells cultivated on coverslips were co-transfected with equimolar amounts of SIDT1/2-GN and SIDT1/2-GC plasmids using Lipofectamine 3000 (Invitrogen). After 24 hours of transfection ...
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bioRxiv - Immunology 2023Quote: ... samples were washed 3X in PBS before incubating 2 hours at room temperature in 2 μg/ml donkey anti-rabbit IgG conjugated to AF594 (CAS: A21207, Thermofisher). After 3 washes in PBS ...
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bioRxiv - Immunology 2024Quote: ... permeabilised with 0.5% Triton x-100 and OPP incorporation into newly synthesised peptides was measured by conjugating with Alexa647-azide via a Click-IT chemistry reaction (2 µM Alexa-647-azide, 2 mM CuSO4, 5 mM ascorbic acid in PBS) (Invitrogen) and assessing fluorescence by flow cytometry.
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bioRxiv - Bioengineering 2024Quote: ... 2 µL of Golden Gate reaction were added to 25 µL of 2× Phusion Polymerase Master Mix (ThermoFisher, Cat# F531L), mixed with 18 µL of H2O ...
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bioRxiv - Immunology 2024Quote: ... or murine IL-2 (30 U/mL, Peprotec) and Dynabeads murine T- activator CD3/CD28 (ThermoFisher, 2 µL per well) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... co-transfection of pX-CRISPR and qTAG repair plasmids was performed at a 1:1 ratio using 4 μg of total DNA (2 μg cut + 2 μg repair) in Opti-MEM Reduced Serum Medium (31985070, Gibco) with Lipofectamine 3000 (L3000008 ...
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bioRxiv - Neuroscience 2024Quote: Intracellular calcium imaging was carried out on isolated taste bud cells loaded with 4 µM of the ratiometric calcium indicator Fura-2-acetoxymethyl ester (Fura-2 AM, Invitrogen) in Tyrode’s with 0.05% pluronic acid F-127 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: 293KbC2 cells were seeded in 6-well plates and transfected with appropriate sample plasmid combinations to equal 2 µg total DNA and 2 µl of Lipofectamine 2000 (ThermoFisher). At 24 h post-transfection ...