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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Molecular Biology 2021Quote: ... Binding of the antibodies was detected using Alexa fluor 633 conjugated anti-human IgG antibodies (Invitrogen). Expression of the surface-displayed myc-tagged RBDs was detected using a FITC conjugated chicken anti-myc antibody (Immunology Consultants Laboratory ...
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bioRxiv - Bioengineering 2021Quote: ... washed with 5 column volumes binding buffer and then eluted with 2.5 mM Desthiobiotin (ThermoFisher, US) in binding buffer on an Äkta Pure FPLC (GE healthcare ...
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bioRxiv - Immunology 2022Quote: ... The cell-surface expression and antigen binding level were determined by anti-kappa FITC (Invitrogen, A18854) and streptavidin PE (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated in binding buffer with Annexin V and/or propidium iodide (Invitrogen, Waltham, MA, USA) for 15 min ...
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bioRxiv - Synthetic Biology 2019Quote: ... The short biotinylated fragment cleaved following nicking was removed by binding to Streptavidin M270 Dynabeads (Invitrogen), and the remaining processed oligos were column-cleaned ...
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bioRxiv - Neuroscience 2020Quote: ... The sodium-sensitive ratiometric dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Schwerte, Germany) was used for detection of Na+ ...
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bioRxiv - Immunology 2021Quote: To confirm RBD-specific binding of the selected nanobodies immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA) were coated with RBD (100 ng per well) ...
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bioRxiv - Immunology 2022Quote: ... to prevent non-specific antibody binding before staining with Zombie Aqua Fixable Viability Dye (Thermo Fisher) and anti-human CD45 antibody (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... NAD or ATP-treated CD4 cells (as described above) were resuspended in annexin-binding buffer (ThermoFisher) and incubated with 1µl 100µg/ml Propidium Iodide (PI ...
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bioRxiv - Cell Biology 2022Quote: ... The specific binding was detected by Super Signal West Femto Maximum Sensitivity Substrate (34096; ThermoFisher Scientific) or Super Signal Dura Extended Duration Substrate (34076 ...
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bioRxiv - Molecular Biology 2022Quote: Cells were re-suspended in 50 µl Lysis/Binding Buffer (from mirVANA kit, Life Technologies AM1560), and 50 µl 0.5 µm zirconium beads (Thistle Scientific 11079105Z ...
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bioRxiv - Genomics 2022Quote: ... washing with cold PBE and binding the DP cells to Dynabeads-sheep anti-rat IgG (Invitrogen). The supernatant was centrifuged to harvest the cells ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (PureLinkTM RNA Micro Kit, Invitrogen), followed by RNA quality assessment (2100 Bioanalyzer system ...
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bioRxiv - Biophysics 2022Quote: ... and incubated with Alexa647- conjugated annexin V diluted 1:50 in annexin V binding buffer (Invitrogen) for 45 min at ambient temperature ...
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Immunology 2023Quote: ... The binding of the conjugate was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific, 32106).
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bioRxiv - Molecular Biology 2023Quote: Cells were re-suspended in 50 µl Lysis/Binding Buffer (from mirVANA kit, Life Technologies AM1560), and 50 µl 0.5 µm zirconium beads (Thistle Scientific 11079105Z ...
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bioRxiv - Cell Biology 2023Quote: ... the antibody binding was visualized using either SuperSignal™ West Pico or Femto Substrate (Thermo Scientific). All information regarding the antibodies used is provided in the supplementary files.
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody binding was revealed using class-specific secondary antibody coupled to Alexa fluorophore (Invitrogen, Germany). All secondary antibodies were used at dilution 1:1000.
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bioRxiv - Biochemistry 2023Quote: Fluorescence polarization DNA-binding data were collected with a Panvera Beacon 2000 fluorescence polarization system (Invitrogen) and analyzed with Prism (Graphpad Software) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were immediately lysed in MagMAX Lysis/Binding Solution Concentrate (Thermo Fisher Scientific AM8500, Waltham, MA) and left in storage at -20°C until all timepoints were collected and ready for automated extraction ...
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bioRxiv - Developmental Biology 2023Quote: ... 5000 live scarb2a+ single cells were sorted into 40 µl of lysis/binding buffer solution (ThermoFisher) containing RNase inhibitor RNasinTM (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were immediately lysed in MagMAX Lysis/Binding Solution Concentrate (Thermo Fisher Scientific AM8500, Waltham, MA) and stored at -20°C until all timepoint samples were ready for RNA extraction ...
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bioRxiv - Cell Biology 2023Quote: ... Binding of the secondary HRP-coupled antibody was detected using a chemiluminescent substrate kit (Thermo Scientific) and recorded with an automated developer machine (Agfa) ...
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bioRxiv - Microbiology 2024Quote: ... To transfer the binding reactions to the nylon membrane (Biodyne™ B Nylon Membrane, Thermo Scientific), we semi-dry transferred the reactions at 15 V for 45 min and immediately crosslinked the transferred DNA to the membrane using a commercial UV-light crosslinking oven (UV Stratalinker ...
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bioRxiv - Immunology 2024Quote: ... Cell proteins and EVs proteins were quantified by Qubit Protein Assay (Invitrogen) using a Qubit 3.0 Fluorometer (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Immunology 2022Quote: ... Ab binding was evaluated by adding 50% diluted tetramethylbenzidine substrate (1-step ultra TMB, #34029, Thermo Scientific). The reaction was terminated by adding equal amounts of 0.2 M H2SO4 (Merck ...
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bioRxiv - Immunology 2022Quote: ... which was followed by labeling in annexin-binding buffer with anti-F4/80 antibodies (1:100, Invitrogen) and annexin V (1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody binding was detected with secondary antibodies: goat anti-mouse (1:2500) (Thermo Fisher Scientific, 62-6520), mouse anti-rabbit (1:3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody binding was detected with secondary antibodies: goat anti-mouse (1:2500) (Thermo Fisher Scientific, 62-6520) and mouse anti-rabbit (1:3000 ...
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bioRxiv - Microbiology 2020Quote: ... The binding of antibodies to the blot was visualized using Pierce ECL Western Blotting Substrate (Thermo Scientific). The indicated relative molecular weight of the protein was deduced from Precision Plus Protein Standards (BioRad).
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bioRxiv - Microbiology 2020Quote: ... The binding of antibodies to the blot was visualized using Pierce ECL Western Blotting Substrate (Thermo Scientific). The indicated relative molecular weight of the protein was deduced from Precision Plus Protein Standards (BioRad).
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bioRxiv - Cell Biology 2019Quote: ... The IgG binding buffer was removed from the beads each time using a magnetic rack (Life technologies). The beads ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated for 15 min at room temperature in the dark in 1x Annexin Binding Buffer (Invitrogen) supplemented with 2 µl Annexin V ...
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bioRxiv - Neuroscience 2019Quote: ... afterwards secondary antibody binding was detected with ECL Western Blotting Detection Reagents (Thermo Fisher Scientific, Waltham, USA) and a Fusion FX7 image and analytics system (Vilber Lourmat ...
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bioRxiv - Developmental Biology 2020Quote: ... 2.5×105 TSCs were seeded into a low binding T25 flask (Nunc, ThermoFisher Scientific, cat no: 169900). Suspended cells were maintained in 3 mL of ST-3D medium containing DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol ...
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bioRxiv - Biophysics 2021Quote: ... the GeneJet DNA spin column-based purification kit and the endotoxin binding reagent were purchased from ThermoFisher Scientific (USA) ...
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bioRxiv - Neuroscience 2021Quote: ... ELISA: The assay was routinely performed in high binding 96-wells plates (Immulon 4 HBX, Thermo Fisher). Washings throughout the assay were ...
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bioRxiv - Plant Biology 2021Quote: ... Collection was performed into 1.5 ml RNase free non-stick Eppendorf low binding tubes (Thermofisher Cat# AM12450) containing 10μl of NIB WASH ...
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bioRxiv - Neuroscience 2020Quote: ... and 1 mM SBFI (Na+-binding benzofuran isophthalate, Teflabs, Austin, TX and Molecular Probes, Eugene, OR, USA) or 0.1 OGB-6F (Ca2+ indicator ...