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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Genetics 2020Quote: ... Intracellular staining (for experiments in which the NF-κB binding site containing rs6927172 was deleted) was performed using the eBioscience Foxp3 / Transcription Factor Staining kit (ThermoFisher) and a Phospho-IkB alpha (Ser32 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Samples were diluted with 400 μL Annexin V binding buffer and acquired on an Attune NxT flow cytometer (ThermoFisher Scientific). A minimum of 10,000 events were acquired in each sample ...
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bioRxiv - Biophysics 2019Quote: ... Cells were then washed three times in binding buffer by centrifugation and subsequently incubated with Alexa Fluor 647 streptavidin conjugate (Invitrogen) for 15 minutes at 4° C ...
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bioRxiv - Bioengineering 2020Quote: ... Epitope confirmation was done by a competitive binding assay where saturating concentration of ICR-10 PE antibody (Thermo Fisher Scientific) was coincubated with a concentration gradient of unlabeled E1-1DD or E4-1DD ...
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bioRxiv - Microbiology 2021Quote: ... The secondary antibody binding was detected by adding 50 μL of tetramethylbenzidine (TMB) peroxidase substrate (cat# 34022, Thermo Fisher Scientific). The peroxidase reaction was terminated by adding 50 μL of H2SO4 (1 M ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated with 1% BSA in PBS for 30 min to block non-specific binding prior to staining F-actin with rhodamine-conjugated phalloidin (Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... were added to 200-400 μl lysis/binding solution in the RNAqueous micro-scale phenol-free total RNA isolation kit (Ambion). Trace contaminating DNA was removed with TURBO DNA-free (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell pellets were re-suspended in binding buffer (500 μL) and stained with Annexin V-FITC and PI using a Dead Cell Apoptosis Kit (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Non-specific binding sites were blocked for 2 h at room temperature with 5% normal SVF (Gibco, ThermoFisher Scientific, France) in 0.1% Triton X-100-PBS ...
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bioRxiv - Cell Biology 2021Quote: ... neuro-retinal vesicles (NRVs) were manually excised using 21G needles/scalpel blades and grown in low-binding 96 well plates (96 well Nunc Sphera Round Bottom Plates ...
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bioRxiv - Cancer Biology 2021Quote: ... The antibodies were diluted in Milk/TBST (5% non-fat dry milk in TBS with 0,1% Tween20) and antibody binding was evaluated using the SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific) or SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... and cloned into the pDEST32 vector containing the GAL4 DNA binding domain (DBD) through Gateway cloning (ThermoFisher, 11789020 and 12538120) and used as the Y2H bait construct ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant obtained after the centrifugation of the lysate at 9000 rpm for 25 minutes at 4°C was subjected to overnight binding with Hi-bind Ni-NTA agarose beads (Invitrogen). The beads were washed 4-5 times with wash buffer (20mM Tris ...
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bioRxiv - Neuroscience 2019Quote: ... The non-specific binding of antibodies was avoided by incubating the cells with 5% normal goat serum(Thermo Fisher Scientific)/ 0.4% Triton X-100(Sigma)/1% Glycine(Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibody binding was visualized on CL-XPosure film using ECL SuperSignal West Extended Duration Substrate (both from Thermo Fisher Scientific) or using the Odyssey CLx Imaging System (LI-COR Biosciences) ...
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bioRxiv - Synthetic Biology 2020Quote: ... flow cytometry and in vivo binding assays) were subcloned via NcoI/EcoRI or HindIII/BamHI into either the pBAD/His A (Invitrogen) or pZA23MCS (EXPRESSYS ...
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bioRxiv - Neuroscience 2021Quote: Messenger RNA was isolated from isolated cells by cell lysis in the lysis/binding buffer and by using oligo(dT)25-linked Dynabeads (Invitrogen). The beads with adherent mRNA were suspended in DEPC-treated water (20 µl) ...
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bioRxiv - Physiology 2021Quote: ... and non-immunological binding of the specific immune sera were blocked by 3% bovine serum albumin (Thermo Fisher Scientific Inc.). The indirect method was introduced to immunohistological staining ...
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bioRxiv - Microbiology 2021Quote: ... and the phosphate concentration is determined from the fluorescence increase through binding of phosphate to the phosphate sensor (0.5 µM; Thermo Fisher). PdeL and c- di-GMP concentrations were used ...
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bioRxiv - Plant Biology 2020Quote: Regions screened for transcription factor binding via Yeast One-Hybrid were first inserted into pENTR 5’TOPO TA entry vector (Thermofisher) and subsequently placed into the pMW2 and pMW3 destination vectors containing HIS3 and LACZ marker genes respectively (Deplancke et al. ...
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bioRxiv - Neuroscience 2019Quote: ... sections were washed 3x in TBS then blocked for non-specific binding in TBS containing 10% normal goat serum (Invitrogen) supplemented with 0.1% Triton-X100 for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... resuspended in 300 μl of Binding Buffer with 2 μg of biotin-labeled tubulin peptide (K40 only, K40-Ac and K40-me3) (ThermoFisher), and samples were rotated at 4 °C for 2 h ...
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Biochemistry 2020Quote: ... of interest were immobilized at defined concentrations (specified in each context) in pH 9.6 carbonate buffer overnight at 4°C onto high-binding plate (Nunc Maxisorp). Next day ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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bioRxiv - Immunology 2020Quote: ... Each well was washed and binding of biotinylated monoclonal antibodies was determined with a 1:30000 dilution of HRP-conjugated streptavidin (ThermoFisher). HRP was detected with TMB and stopped with 1% HCl ...
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bioRxiv - Immunology 2021Quote: The COVID-19 receptor-binding domain (RBD) and the N-terminal peptidase domain of human ACE2 were expressed using HEK293F cells (Invitrogen). The COVID-19 RBD (residues Arg319-Phe541 ...
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bioRxiv - Biochemistry 2022Quote: ... were used to generate an amplicon of the insert containing T7 RNA polymerase binding sites from which gene-specific dsRNAs were synthesised by in vitro transcription (T7 Megascript, Thermofisher). DNase1-digested dsRNAs were purified by phenol/chloroform extraction ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were added with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 µg mL-1 in PBS, Invitrogen) and phalloidin conjugated Alexa Fluor dye (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2022Quote: ... The samples from each species were pooled and filter-sterilized through a 0.22 µm low-binding PES membrane using Rapid Flow Rapid-Flow Sterile Disposable Filter Units (Nalgene/Thermo Scientific). The samples were frozen at −80 °C and freeze dried ...
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bioRxiv - Microbiology 2022Quote: ... in PBS were added to the wells of streptavidin-coated high binding capacity 96-well plates (Thermo Fisher Scientific, USA). After incubation overnight at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... The beads were freed from storing solution by magnetic separation and then the antibody conjugation performed by adding 200µl of the Ab Binding Buffer (Thermo Fisher), and 5 µg of DCTN1 antibody ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions (5 × 105 - 2 × 106 total cells) were washed with PBS and resuspended in 1X Annexin binding buffer (Thermofisher) and washed once ...
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bioRxiv - Biophysics 2023Quote: ... the samples were added with a DNA-binding dye 4’,6-diamidino-2-phenylindole (DAPI, 1 μg mL-1 in PBS, Invitrogen) along with the secondary antibody solution ...
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bioRxiv - Immunology 2023Quote: ... Recombinant S309 Fab used for SPR binding studies was produced in ExpiCHO-S cells and purified using a Capture Select CH1-XL MiniChrom Column (ThermoFisher), followed by buffer exchange into PBS using a HiPrep 26/10 Desalting Column (Cytiva).
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bioRxiv - Biochemistry 2023Quote: ... Binding capacity Ni-beads 40 mg/ml medium) was mixed with Alexa Fluor 488-C5-maleimide (AF488, Thermo Fisher Scientific) (final concentration 10 µM ...
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bioRxiv - Cell Biology 2022Quote: ... KATNAL1 and KATNB1 and their binding partners were then co-immunoprecipitated using the Pierce Co-immunoprecipitation Kit (Thermo Scientific, 26149) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Cells were collected following transfection incubations and resuspended in Annexin V binding buffer and Annexin V AlexaFluore 350 conjugate (Thermofisher) or Propridium Iodide (PI ...
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bioRxiv - Immunology 2023Quote: Non-specific Fc binding was blocked for 10 minutes on ice with unconjugated anti-CD16/CD32 (93; Thermo-Fisher Scientific). Cells were subsequently labeled for 30 minutes on ice with the following antibodies and reagents ...
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bioRxiv - Pathology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Immunology 2024Quote: Harvested supernatant was diluted 2× in Tris-Hcl 50 mM NaCl 150 mM CaCl2 2 mM pH 8.0 (binding buffer) and incubated with immobilized p-aminophenyl phosphoryl choline beads (ThermoFisher Scientific) at room temperature for one hour ...
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bioRxiv - Biochemistry 2023Quote: ... Cross-linked samples were divided into 12 eppendorf tubes with each tube containing 200 µl of the cross-linked sucrose gradient sample mixed with 300 µl of the lysis/binding buffer from the mirVana kit (Invitrogen), 600 µl of acid-phenol ...
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bioRxiv - Pathology 2023Quote: ... Binding of the primary antibody was detected by an enhanced chemiluminescence method (SuperSignal™ West Femto, 34094, Thermo Fisher Scientific) using horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit IgG ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody binding was detected by a goat-anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (1:2000 dilution; ThermoFisher). Signals were detected using a chemiluminescence substrate and the Azure c600 Imaging System (Azure Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...