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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Retinol Binding Protein RBP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Hb binding partners were identified by ESI-LC-MS/MS mass spectrometry (LTQ-Orbitrap Velos, Thermo Fisher Scientific) using Proteome Discoverer 1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Assays for concentration-dependent RBD binding (Fig. 1E) were developed using 1-step Slow TMB (Thermo Fisher Scientific). EC50 was determined from non-linear regression models using Graph Pad Prism 8.
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bioRxiv - Genomics 2021Quote: ... followed by adding 100 μl of Lysis/Binding Buffer in Dynabeads® mRNA DIRECT™ Micro Kit (Invitrogen) and pipetting up and down to lyse the cells ...
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bioRxiv - Genetics 2020Quote: ... Cells were washed once in PBS and then washed once in 1X Annexin-V Binding Buffer (ABB) (Invitrogen). The cells were resuspended at a density of 1 x 106 cells/ml in 1X ABB ...
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... an LR reaction was used to shuttle these multimerized binding sites into the pGREEN binary described above (Invitrogen). Cloning primer sequences for all constructs are listed in Table S7 ...
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bioRxiv - Molecular Biology 2023Quote: ... and nascent RNAs were isolated by Streptavidin bead binding (Dynabeads® MyOneTM Streptavidin T1 magnetic beads, ThermoFisher Scientific). Following 3’ adapter ligation ...
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bioRxiv - Microbiology 2023Quote: ... The water samples were stained with the DNA-binding fluorescent dye SYBR green I (Invitrogen, final concentration 2x), vortexed and incubated for 15 minutes in the dark at 37° C ...
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bioRxiv - Biophysics 2023Quote: ... Blots were rinsed in distilled water and antibody binding was detected with chemiluminescent solution (Pierce, Fisher Scientific, France) and visualized under a ChemiDoc MP imaging system (Biorad ...
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bioRxiv - Developmental Biology 2023Quote: ... PGCLCs were aggregated with isolated gonadal somatic cells in a low-binding 96-well plate (174929, Thermo Scientific) for 2 days in GK15 medium supplemented with 1 μM retinoic acid ...
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bioRxiv - Microbiology 2023Quote: ... Soluble EFNB2-Fc was constructed by cloning the receptor binding domain (a.a. I28 to G165) of EFNB2 into the NheI-BamHI sites of pcDNA3.1(+) (Invitrogen) with a N-terminal CD5 leader (MPMGSLQPLATLYLLGMLVASVLA ...
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bioRxiv - Biochemistry 2023Quote: ... and the binding of each clone to SN-38 were analyzed using the Attune flow cytometer (Thermo Fisher). The sequences with the best binding signals were tested for binding using BLI.
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bioRxiv - Cancer Biology 2023Quote: GTP-bound RAC1 was detected using PAK1-p21-binding domain (PBD) pull-down and Detection Kit (Thermo Scientific) as instructed by the manufacturers.
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bioRxiv - Microbiology 2023Quote: ... Binding of primary antibody was visualized using Alexa 488 α-rabbit or 568 α-mouse secondary antibody (Invitrogen). 4’,6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2023Quote: ... for 30 min and non-specific binding sites were blocked with Ultra-V Block solution (Thermo Fisher Scientific) for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... pH 7.4 and added (100μL/well) to 96-well microtiter plates with high binding capacity (Thermo Fisher, USA) and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... were added to the binding reactions followed by 1.2 µL of diluted DNAse I (0.625 U total, Invitrogen) and reactions were incubated at room temperature for 5 minutes ...
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bioRxiv - Genomics 2024Quote: A vector containing the Streptavidin-Binding Peptide (SBP)-Tag was prepared in pEF1 (Thermo Fisher, Cat# V 92120), and SERBP1 ORF was cloned in frame with the His-Myc-tag present in the vector to create pSBP-SERBP1 ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
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bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
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bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... Corneas were transferred from RNAlater to 0.7 mL of lysis/binding buffer from the mirVana miRNA isolation kit (Ambion) and homogenized for 1 minute using a tissue tearer ...
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bioRxiv - Developmental Biology 2021Quote: ... decapsulated and transferred in low-binding Eppendorf tube containing 100-150µL collagenase solution in HBSS (Collagenase type IV (Gibco), 2X AAs (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Molecular Biology 2021Quote: ... the cleared supernatant was loaded onto equilibrated Streptactin resin (IBA) supplemented with 60 μL of BioLock (IBA) to prevent unspecific binding and 0.1 U/μL Ribolock (Invitrogen) to inhibit RNA decay ...
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bioRxiv - Microbiology 2019Quote: ... The binding assays and detection of RNA products were performed with the LightShift Chemiluminescent RNA EMSA Kit (Thermo Scientific). For the control reactions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were harvested and washed once with PBS and once with 1x Annexin V binding buffer (ThermoFisher Scientific, V13246). Following washes ...
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bioRxiv - Bioengineering 2019Quote: STP and mAb binding to the carriers was quantified indirectly using a bicinchoninic acid assay (BCA) kit (Thermo Fisher) according to the manufacture’s instructions ...
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bioRxiv - Immunology 2021Quote: Monocyte-derived macrophages and DCs were lysed upon collection with MagMAX(tm) Lysis/Binding Solution (Invitrogen, Thermo Fisher Scientific) and frozen in -80 °C ...
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bioRxiv - Neuroscience 2020Quote: ... we began by covalently binding anti-GFP antibodies (19C8,19F7, gift from Eric Schmitt, Rockefeller Univ) to magnetic Dynabeads (Invitrogen), and treating with IgG-free BSA to reduce background binding ...
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bioRxiv - Biochemistry 2020Quote: ... The microarray slides were probed with Fc-tagged DC-SIGN and L-SIGN at 10 μg/ml diluted in 1% BSA (TSM with 0.05% Tween) and binding was detected with Alexa Fluor 488 labelled goat anti-human IgG (H+L) (Invitrogen) at 5 μg/ml.
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bioRxiv - Immunology 2021Quote: Monocyte-derived macrophages and DCs were lysed upon collection with MagMAX(tm) Lysis/Binding Solution (Invitrogen, Thermo Fisher Scientific) and frozen in -80 °C ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... Binding of SYBR green to DNA was analysed in a QuantStudio 5 Real-Time PCR system (Thermo Fisher Scientific). Reaction conditions were as follows ...