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Citations for Thermo Fisher :
4401 - 4450 of 7785 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... cells were washed thrice with TBST (20 min each) and mounted using Prolong antifade Gold mountant (Thermo Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... Zygotes were piezo-electric microinjected in KSOM medium supplemented with 20% fetal bovine serum (Gibco BRL, Gaithersburg, USA) by aspirating an amount of the RNP complexes ...
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bioRxiv - Physiology 2022Quote: Total RNA from human liver biopsies (10-20 mg) was extracted using Trizol extraction (Life Technologies, Carlsbad, CA) followed by a purification step using the GeneJET RNA Purification kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 11 and 20 and enriched by FACS into collection tubes containing Trizol LS Reagent (Thermo Fisher Scientific, 10296010). Total RNA from 60,000 sorted cells/sample was isolated via the precipitation method in combination with spin-columns using PureLink RNA Micro Kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... 20 ng of cDNA was used per qPCR reaction using Powerup SYBR green master mix (Thermo Fisher Scientific) and gene specific primers according to manufacturer’s instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... cells were stained for 20 min on ice with LIVE/DEAD Fixable Blue Dead Cell Stain Kit (Invitrogen) before the surface antibody staining ...
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bioRxiv - Neuroscience 2022Quote: ... internal solution was supplemented with 20-150 μM Alexa Fluor 488 and/or Alexa Fluor 594 (Life Technologies).
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bioRxiv - Genomics 2022Quote: ... 100 ng of plasmid DNA were incubated for 20 minutes with 0.15 μl Lipofectamine 3000 and 0.2 μl P3000 transfection reagent (Invitrogen) in 10 μl RPMI-1640 ...
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bioRxiv - Biophysics 2022Quote: ... Rather than purified sensor,20 μl of a 1/10,000 dilution of carboxylate latex fluorescent beads (Fluospheres, Invitrogen) of either 20 nm (F-8786) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and samples were digested overnight at 37°C with 20 μg sequencing grade trypsin (90057, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... and then immediately injected into a 20-ml pre-reduced and autoclaved HDSP vial (C4020-201, Thermo Scientific) sealed with 20-mm Crimp Cap (95025-01-1S ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... The screening of the capsids was carried on FEI Tecnai G2 20 electron microscope (ThermoFisher Scientific, United States) at 200 kV accelerating voltage ...
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bioRxiv - Biochemistry 2020Quote: ... The digestion process was stopped by the addition of Dulbecco’s Modified Eagle’s Medium (DMEM) containing 20% fetal bovine serum (FBS) and 100 units/mL penicillin (Gibco), 100 μg/mL streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: ... 20 mm and 25 mm TL stages were incorporated into 3D visualization and analysis software Amira (Thermo Fisher) and the positions of all sections were manually aligned ...
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bioRxiv - Molecular Biology 2021Quote: All cells were transfected with siRNAs for a 20 nM final concentration using Lipofectamine RNAimax (Invitrogen™, 13778030) when plated ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in 20 μl of loading buffer and run on a 4-12% NuPAGE Bis-Tris gel (Invitrogen) in MOPS buffer according to the manufac turer's instructions ...
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bioRxiv - Molecular Biology 2020Quote: RT-RCA was performed in 20 µL with Maxima H Minus Reverse Transcriptase (Thermo Fisher, cat n°EP0752) under the following conditions ...
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bioRxiv - Physiology 2020Quote: ... Control animals were kept at 20°C in a Low Temperature Incubator (Thermo Fisher Scientific, Waltham, MA, USA), humidified to prevent evaporation ...
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bioRxiv - Synthetic Biology 2021Quote: ... Twenty micrograms of each peptide were incubated with 20 µl of pre-washed Dynabeads M-280 Streptavidin (Invitrogen) at RT for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... Denatured protein samples were loaded onto 4-20% Tris-Glycine Gel Novex WedgeWell (Invitrogen; Grand Island, NY, USA) for gel electrophoresis ...
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bioRxiv - Molecular Biology 2021Quote: ... The 20 μL qPCR reaction was assembled from 10 μL Platinum Multiplex PCR Master Mix (Life Technologies, #4464269), 1.5 μL N2 primer set (2019-nCoV RUO Kit ...
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bioRxiv - Microbiology 2020Quote: ... 20 µL were analysed by nanoLC-ESI-MS/MS using the Ultimate 3000/Orbitrap Fusion instrumentation (Thermo Scientific), and a 90-minute LC separation on a 50 cm column ...
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bioRxiv - Microbiology 2020Quote: ... NIH-3T3 cells (ATCC CRL-1658) and NIH-3T3-ORF50 cells [20] were grown in Dulbecco’s modified Eagle’s medium (Gibco) with 2mM glutamine ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 4 mM DTT and 20% glycerol) at 30 °C in a Cytation 5 imaging reader (Thermo Fisher Scientific) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Cell Biology 2020Quote: ... equal amounts of protein (20 μg) were separated under reducing conditions on 4-12 % Novex WedgeWell gels (Invitrogen) and transferred to PVDF membrane (Advansta) ...
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bioRxiv - Immunology 2020Quote: ... 20⧅l of opsonised Escherichia coli (E.coli) was added to the tube (pHrodo Red E. coli BioParticles; ThermoFisher). The bacteria was initially prepared by mixing the E.coli strain with new-born calf sera followed by a 30-minute incubation at 37°C ...
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bioRxiv - Genetics 2020Quote: ... passed through a 0.4μm cell strainer and suspended in DMEM/L-15 medium supplemented with 20% knockout serum replacement (Invitrogen), 2mM L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... and the pellets were frozen at -20°C overnight before resuspension in 8.5 μl B-PERII (Thermo Scientific) and incubation for 1 h at room temperature with shaking (600 rpm) ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were flicked and cells were incubated with 20 μl peptide diluted in HBSS buffer (Invitrogen 14065-049) complemented with 20 mM HEPES (Invitrogen 15630-056 ...
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bioRxiv - Physiology 2020Quote: ... Quantitative PCR reactions were performed on 10-20 ng cDNA using SYBR Select Master Mix (Thermo Fisher Scientific) with a Bio-Rad CFX Connect Real-Time PCR Detection System ...
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bioRxiv - Neuroscience 2020Quote: ... Serial coronal sections (20 μm) of the brain were obtained using a cryostat (Microm 560 M, Thermo Fisher). Tissue was first incubated with a blocking solution containing PBS ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant was removed and cells re-suspended in 90μl of Schneider’s medium containing 20% fetal calf serum (Gibco). 30μl drops were placed on cover slips ...
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bioRxiv - Neuroscience 2021Quote: ... 0.8 mM KH2PO4, 20 mM NaHCO3, 1.3 mM L-glutamine, 0.2 mM ascorbic acid, MEM amino acids solution [Gibco; Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... for a total volume of 20 μl: 4 μl of 5x First-Strand SuperScript III buffer (Invitrogen, 18080044), 2 μl of 0.1 M DTT (Invitrogen ...
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Empowering Engineered Muscle Function by Extending Connexin 43 Duration with Reduced Graphene OxidesbioRxiv - Bioengineering 2021Quote: ... Cells incubated with MF-20 were treated with Alexa Fluor-488 goat anti-mouse IgG (1:500; Invitrogen), and those incubated with anti-sarcomeric α-actinin antibody were treated with Alexa Fluor-568 donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked in 3 % nonfat milk in PBS with 0.1% Tween 20 and incubated in the same buffer with HRP-conjugated streptavidin (1:5,000; Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ~20 mg flash-frozen tumor fragments were weighed out and homogenized in 1 ml TRIzol (Invitrogen #15596-026) in Precellys tissue homogenizing mixed beads kit (Cayman Chemical #10409) ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were rehydrated into PBS containing 0.1% Tween-20 prior to performing EdU Click-It reactions (Thermo Fisher) as described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Cells used in this study were within 20 passages after thawing and were cultured (37°C, 5% CO2) in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... RBCs were centrifuged and the pellet resuspended either in 20 μM carboxfluorescein diacetate succinimidyl ester (CFDA-SE) (Invitrogen), or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were washed with PBST (0.1% Tween-20) and imaged with chemiluminescence reagent (Thermo Fisher Scientific Inc., 34095). Densitometry analyses were performed using ImageJ software.
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bioRxiv - Immunology 2020Quote: ... was mixed with 15 µl of 20 times diluted SYPRO orange fluorescent dye (5000 x stock, Invitrogen S6650) in a 96-well optical qPCR plate ...
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bioRxiv - Immunology 2021Quote: ... were used with 20 ng genomic DNA and the appropriate amounts of TaqMan Universal MasterMix II (Thermo Fisher) in a 10 µl reaction volume run in triplicate wells of a 96 well MicroAmp plate run on a QuantStudio 7 qPCR cycler (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... Caco-2WT and Caco-2ΔHk2 cells were cultured in MEM with 20% (v/v) FCS purchased from Gibco/Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Microbiology 2021Quote: ... the OD600 of the culture was determined using a GENESYS™ 20 spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany) and a sample (1 mL ...
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bioRxiv - Microbiology 2021Quote: ... RNA was resuspended in 20-50 µl of DEPC water and quantified using a NanoDrop (Thermo Fisher Scientific).
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bioRxiv - Genomics 2021Quote: ... Primary human T cells were cultured for up to 20 days in ATCC-modified RPMI (Thermo Fisher, #A1049101) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... the frozen samples were cryosectioned at - 20 °C with a microtome (Microm HM 550, Thermo Fisher, Waltham, USA) into 16 µm thick slices and placed onto calcium fluoride windows (CaF2 ...
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bioRxiv - Cell Biology 2021Quote: ... and the samples were stained for 20 min with 40 μl 2X SYBR Green DNA stain (Invitrogen S7563) and then washed twice in 200 μl PBS ...