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Citations for Thermo Fisher :
4251 - 4300 of 7785 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 20 nM siRNAs using Lipofectamine™ RNAiMAX Transfection Reagent (Thermo Fisher Scientific, 13778150) according the manufacturer’s instructions and imaged or harvested after 3 or 4 days post transfection (dpt) ...
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bioRxiv - Plant Biology 2024Quote: ... 200 ng of total RNA were treated with 20 ng/µL of RNase A (Thermo Scientific, EN0531) diluted in 1x reaction buffer (15 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: Proteins were transferred to PVDF membranes (0.2um pore) using 20% methanol in Nupage transfer buffer (Thermo Fisher) with antioxidant (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture was then separated into two 20 μL aliquots and 1 mL of F12 (Thermo Scientific) media was added to each aliquot ...
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bioRxiv - Biochemistry 2024Quote: The SEC peak elution fractions were pooled in a 20-kDa MW cutoff cassettes (Thermo Fisher Scientific), incubated with ProTEV Plus protease (Promega) ...
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bioRxiv - Biochemistry 2024Quote: The processed streptavidin beads samples were mixed with 20 µL of 2X loading buffer (Thermo Fisher Scientific) and heated at 98℃ for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and briefly centrifuged for 1 minute at 1000 rpm using a M-20 rotor (Thermo Scientific, 75003624) in a tabletop centrifuge (Legend X1R ...
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bioRxiv - Immunology 2024Quote: ... fixed for 20 min with 2% PFA in PBS and washed/permeabilised in 1x Permeabilisation buffer (Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and acetylated tubulin staining: 2 mL blocking buffer containing 20 μL anti-Sox2 (Invitrogen, 14-9811-82), 20 μL anti-Myt1 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... using oligo(dT)20 primers provided within SuperScript™ IV First-Strand Synthesis System (ThermoFisher Scientific # 18091050) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 20% FBS and 100 IU/mL penicillin and 100 ug/mL streptomycin (Gibco or Corning) and passaged every 2-3 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: After 20 hours of LPS stimulation cell viability was assayed by adding AlamarBlue™ (Invitrogen, Massachussets, USA) to each well ...
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bioRxiv - Neuroscience 2024Quote: ... 20 µL of total protein from brain extracts was immunoprecipitated using 50 µL M-280 Dynabeads (Invitrogen) coated with 4 µg 77G7 anti-tau antibody (MTBR epitope 316-355 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then treated with the indicated concentrations of RSL3 and 20 nM Sytox Green (Invitrogen, S7020). The number of live and dead cells present after 24 h was monitored using imaging with the Incucyte Live-Cell Analysis System (Sartorius).
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bioRxiv - Neuroscience 2024Quote: ... or with an EVOS M7000 Imaging System microscope with a 20×/0.4 numerical aperture (Thermo Fisher Scientific). For quantification of p21- ...
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bioRxiv - Molecular Biology 2024Quote: ... and RNA was precipitated from the aqueous phase using ice-cold isopropanol with 20 μg glycogen (Invitrogen) by incubation on ice for 30 min to 1 h ...
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bioRxiv - Immunology 2024Quote: Mice were euthanized in a CO2 chamber and immediately perfused with 20 mL sterile 1x PBS (Gibco). The gallbladder was removed ...
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bioRxiv - Neuroscience 2024Quote: ... a subset of smaller molecular weight proteins were tested on 4-20% tris glycine gradient gels (Invitrogen). Proteins were transferred to 0.2 mm nitrocellulose membranes using semi-dry transfer (Bio-Rad Transblot Turbo ...
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bioRxiv - Neuroscience 2024Quote: ... cells were incubated with 20 μM H2DCFDA (5,6-chloromethyl-2’,7’-dichlorodihydro fluorescein diacetate, acetyl ester, Invitrogen) for 6 min at 37 ⁰C as described [44] ...
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bioRxiv - Biophysics 2024Quote: ... For primary rat astrocytes DMEM supplemented with 20% FBS was used for culturing and accutase (Thermo Fisher) served as the cell dissociation reagent ...
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bioRxiv - Bioengineering 2024Quote: ... Monosaccharides in hydrolysates were separated by a CarboPac PA-20 column (3 × 150 mm; Thermo Fisher Scientific) using a program described previously71 ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue slices were blocked for 2 h at room temperature with 20% Fetal Bovine Serum (FBS, Gibco) and 20% Normal Goat Serum (NGS ...
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bioRxiv - Biochemistry 2024Quote: ... the sample was transferred to a pre-wetted dialysis cup (20 kDa MWCO, Thermo Scientific™, 69590) and dialyzed overnight into a large volume of PBS at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Media was changed 24 hours later to 20 mL viral production medium: IMDM (Thermo Fisher Scientific #1244053) supplemented with 20% heat-inactivated fetal bovine serum (GeminiBio #100-106) ...
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bioRxiv - Biochemistry 2024Quote: ... The cleared extract was incubated 20 minutes with 2 ml pre-equilibrated Ni-NTA resin (Thermo Scientific) and washed extensively with a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... followed by incubation with 5 μL of 20 mg/mL Proteinase K (Thermo Fisher, catalog no. FEREO0491) at 50 °C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 58°C for 30 seconds and 72°C for 20 seconds on SimpliAmp Thermo Cycler (Applied Biosystems). PCR products were visualized on a 2% TAE agarose gel stained with ethidium bromide.
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bioRxiv - Bioengineering 2024Quote: ... Cells in both blood and brain channels were incubated with Hoechst 33342 (20 µM Thermo Fisher Scientific) and CellEvent™ caspase-3/7 green detection reagent (2 µM Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... cell supernatants were collected and concentrated 20-fold using Pierce™ Protein Concentrators PES (cat# 88528, Thermofisher). The concentrated supernatants were then assayed using the IL-33 (cat# TBS3245 ...
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bioRxiv - Cancer Biology 2024Quote: RNA isolation from 20 mg of adipose tissue was performed using TRI Reagent® (ThermoFisher. Cat# AM9738) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained cells were cultured on non-coated dishes in 20% FBS (Biowest) in F10 (Thermo Fisher) supplemented with 2.5 ng/mL bFGF (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2024Quote: ... Needle wash steps were performed with Acetonitrile:Isopropanol:H2O (40:40:20, v/v/v) (ThermoFisher, 10055454) (ThermoFisher, 10684355). Mobile phase A was comprised of H2O and 0.2% formic acid (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were next transfected 6h after with 20 nM silencer select negative control siRNA1 (4390843, Thermo Fisher) or siCCDC77 (s38908 ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured them in the induction medium (DMEM/F12 containing 20% knockout serum replacement (ThermoFisher Scientific, 10828010), 1 mM MEM non-essential amino acids (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... or with 1 µl of p50 antibody together with 20 µl of Protein A magnetic beads (Life Technologies). After 4 washes in IP buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 μL of each sample was run on a NuPAGE 4-12% Bis-This protein gel from Invitrogen and then transferred to PVDF membrane from EMD Millipore ...
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bioRxiv - Cell Biology 2020Quote: U2OS-HTTQ91mCherry cells were trypsinized and reverse transfected with 20 nM siRNA by using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s protocol in 6 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... 20 mM dithiothreitol (DTT) and bromophenol blue 1.5 mM) were resolved on NuPAGE 4%-12% gradient gels (Invitrogen), and analyzed by standard immunoblotting techniques ...
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bioRxiv - Immunology 2021Quote: ... K63-SUB-bound beads were prepared by incubating 20 µl/dish Streptavidin Magnetic Agarose beads (Thermo Fisher 88817) with 5 µg/dish recombinant biotinylated K63-SUB ...
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bioRxiv - Microbiology 2021Quote: ... RNA was resuspended in 20-50 μl of DEPC water and quantified using a NanoDrop (Thermo Fisher Scientific).
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bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
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bioRxiv - Genomics 2021Quote: ... previously assembled from four oligonucleotides and diluted 1:20) and 1 μl of T4 DNA ligase (Thermo Scientific) and incubated for 4h at 22ºC (as described in (7)) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10%–20% confluent) were transfected with 10 nM Silencer Select Pre-designed siRNA targeting EXOSC3 (Ambion, Life Technologies) using Lipofectamine RNAi MAX (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... 20 units/ml DNAse I) supplemented with 0.01 μM Sytox red (SYTOX™ Red Dead Cell Stain; Invitrogen). Just prior to FACS ...
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bioRxiv - Developmental Biology 2021Quote: ... 1% TritonX-100) containing 100 μg/ml of CHX and 20 U/ml of Ribolock (Thermo Fisher Scientific). After disruption of the zona pellucida with 250 μl of zirconia-silica beads (BioSpec) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2,000 PαS cells were cultured in a 6-well plate with 20% FBS (mesenchymal stem cell-qualified, Gibco), 25 units/mL penicillin/streptomycin and 2 mM L-glutamine in alpha MEM (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Gel shifted products were resolved on 4-20% TBE PAGE and DNA stained with Sybr Gold (Thermofisher Scientific). For fluorescence polarization experiments measuring DNA binding ...
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bioRxiv - Immunology 2022Quote: ... 20 μg was loaded onto 10% Tris-Glycine gels and separated using the XCell SureLock Blot Module (Invitrogen). Gels were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2022Quote: ... with 200 μl serum-free DMEM/F12 medium supplemented with 20 ng/mL basic fibroblast growth factor (Invitrogen), 10 ng/mL epidermal growth factor (BioSource) ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored by measuring OD600 every 15-30 minutes using a GENESYS 20 spectrophotometer (Thermo Scientific). Cultures were briefly mixed by hand before each measurement ...