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Citations for Thermo Fisher :
4301 - 4350 of 7785 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Brains were transferred directly from this solution into 20 ul of Slow-Fade Gold Antifade Mountant (Invitrogen, S36936) on glass slides with coverslip bridges (Number 2-170 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... we used 20-30 ng of cDNA and the reaction mixture with SYBR Green nucleic acid staining (Invitrogen) on a CFX96 of CFX384 Real-Time systems (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral transduction was facilitated by centrifugation (2500 rpm, 1.5 h at 20°C, Sorvall Legend XTR, Thermo Scientific), after which cells were incubated in polybrene-containing media for an additional 6 h before being returned to regular B cell activation media ...
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bioRxiv - Bioengineering 2022Quote: ... cells were blocked with buffer solution (3% wt/v BSA and 0.1% v/v Tween 20 (Fisher Scientific)) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... A single cell suspension was obtained by a 20 minute incubation of organoids in TrypLE Express solution (Gibco) with DNase I (0.1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in a 20 μl reaction volume using Platinum Taq High Fidelity polymerase (Thermo Fisher Scientific) and PCR primers 275F (5’-ACA GGGACCTGAAAGCGAAAG-3’ ...
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bioRxiv - Bioengineering 2020Quote: ... and a C18 nanoViper Trap Column (20 mm x 75 μm, 3 μm, 100 Å; Thermo Fisher Scientific), connected to an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated for 20 min in 200 μl of NeuroTrace 500/525 Green Fluorescent Nissl Stain (Molecular Probes), diluted 1:100 in PBS before use ...
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bioRxiv - Pathology 2020Quote: ... Three separate 20 µL RT-qPCR reactions were set up in a 384-well Barcoded plate (Thermo Scientific) for either the N1 ...
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bioRxiv - Genomics 2020Quote: ... Sonicated chromatin was diluted in 1X TBST+PI (25 mM Tris-Cl pH 8.0, 0.15 M NaCl, 0.05% Tween-20, and 1X Pierce Protease Inhibitors [Thermo Fisher]). For input libraries ...
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bioRxiv - Evolutionary Biology 2020Quote: ... × 20 mm trap column and 75 μm × 15 cm analytical column (3μm/100A, Thermo Fisher Scientific, Bremen, Germany). Peptides were separated using an 80-minute linear gradient ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA was digested with the addition of 0.5 μL 20 mg/mL RNase A (Fisher Scientific, Waltham MA) and incubation at 65°C for 30 minutes.
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bioRxiv - Evolutionary Biology 2021Quote: ... RNA was digested with the addition of 0.5 µL 20 mg/ml RNase A (ThermoFisher Scientific, Waltham MA) and incubation at 37°C for 1 hour or at room temperature overnight.
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bioRxiv - Cancer Biology 2020Quote: Cryosections were made at −20°C to −16°C using a Cryostar NX (Thermo Scientific, Waltham, MA, USA) disinfected with Leica Cryofect Desinfectant Spray (Leica ...
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bioRxiv - Immunology 2021Quote: ... in a final reaction volume of 20 μL using Platinum Taq High Fidelity polymerase (Invitrogen, #11-304-029) and PCR primers envB5out (5’-TAGAGCCCTGGAAGCATCCAGGAAG-3’ ...
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bioRxiv - Immunology 2021Quote: ... Reactions were formed in 20 μL reaction volume using Platinum Taq High Fidelity polymerase (Invitrogen, #11-304-029) and PCR primers 275F (5’-ACAGGGACCTGAAAGCGAAAG-3’ ...
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bioRxiv - Plant Biology 2020Quote: ... 1 second on/off cycles for 20 s total on ice (Fisher Scientific model FB505, with microtip probe). Extracts were clarified by centrifugation twice at 4°C for 10 min at ≥20,000xg ...
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bioRxiv - Neuroscience 2020Quote: ... 20 sec 72 °C) with fluorescent readings during annealing and elongation on a QuantStudio 3 machine (Thermo Fisher). Three reference genes were tested ...
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bioRxiv - Microbiology 2021Quote: ... 10–20 μg total protein was supplemented with 50 mM DTT and 4X LDS sample buffer (Life Technologies). Samples were heated to 70°C for 10 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 µg of RsaI/HinfI digested DNA was digested with 100 U of S1 nuclease (Thermo Scientific #EN0321) for 45 min at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Peptides were concentrated using a trap column (0.075 × 20 mm, 3 µm, Acclaim PepMap 100 C18, Thermo Scientific) and then separated using a nano capillary column (0.1 × 150 mm ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed as previously described (20) on a StepOnePlus Real-Time PCR System (Applied Biosystems). Relative quantity of transcripts was calculated as 2-ΔΔCt ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots representing 40 μg total proteins were separated on Novex™ 4-20% Tris-Glycine Mini Gels (Invitrogen) or 4-20% Criterion™ TGX Stain-Free™ Protein gels (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... 20-40 mg of total RNA was incubated with 6 units of TURBOTM-DNase (Thermo Fisher Scientific AM2238), 1X TURBO™DNase buffer ...
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bioRxiv - Microbiology 2020Quote: ... Half of the eluate (20 μl) was treated with 1 μl RNase Cocktail Enzyme Mix (Thermo Fisher Scientific) at 37°C for 30 min.
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bioRxiv - Bioengineering 2021Quote: ... Culture media contained Gibco minimum essential medium (MEM-α) supplemented with 20 v/v% fetal bovine serum (Gibco) and 1 v/v% penicillin/streptomycin/amphotericin B (1000 U/mL ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were carried out in triplicate 20 μL volumes of 10 μL Sybr Green Master Mix (Applied Biosystems), 30 ng cDNA ...
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bioRxiv - Bioengineering 2020Quote: 20 µL of 1.33 mg/mL solution of BSA-maleimide (Imject™ Maleimide-Activated BSA, ThermoFisher Scientific #77115) in water ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg RNA was used to prepare cDNA using random primers (total reaction volume = 20 μL; Applied Biosystems High-Capacity Reverse Transcription Kit) ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were loaded into an Acclaim PepMap 100 C18 trap column (0.2 mm × 20 mm, Thermo Fisher Scientific) with a flow rate of 5 μ l/min and separated on an EASY-Spray HPLC Column (75 μ m × 150 mm ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched by adding EDTA (17 mM final concentration) and incubated with 20 μg Proteinase K (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein samples were size fractionated on Novex 4-20% Tris-Glycine Mini Gels (Thermo Fisher Scientific XP04200BOX) at room temperature and subsequently transferred overnight onto Immuno-Blot PVDF membranes (BioRad 1620177 ...
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bioRxiv - Biochemistry 2020Quote: ... Sample was then added to a 20 kDa MWCO Slide-A-Lyzer MINI dialysis device (Thermo Fisher Scientific) and dialyzed against three buffer changes (the second one being overnight ...
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bioRxiv - Cell Biology 2021Quote: ... for 5min and then with hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µl of EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Fisher Scientific, 1 µg/20 µl), while still attached via the umbilical cord to the mother’s blood circulation ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 20 minute incubation with Alexa 647- conjugated phalloidin (1:300 in PBS; Life Technologies No. A22287), when appropriate ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were probed in PBST (PBS pH 7,4, 0,05% (v/v) Tween-20) with the mouse anti-his antibody (1:3000, Invitrogen) and the respective secondary antibody goat anti-mouse-HRP (1:3000 ...
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bioRxiv - Systems Biology 2020Quote: ... Each pellet was resuspended by pipette in 180 μL of enzymatic lysis buffer (20 mM Tris-HCl (Invitrogen), 2 mM Sodium EDTA (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature for 20-30 min and mounted on slides with ProLong Gold Antifade Mountant (ThermoFisher #P36934). Primary antibodies included chicken anti-GFP (ThermoFisher #A10262 ...
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bioRxiv - Microbiology 2020Quote: ... WT and TLR3-/- macrophages were treated with 20 μg/mL Poly (I:C) alone or Poly (I:C) was transfected into cells by using Lipofectamine 2000 (Invitrogen) as the transfection reagent ...
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bioRxiv - Microbiology 2020Quote: ... 20 μg of bacterial RNA were treated with DNase I (Invitrogen™ Ambion™ TURBO DNA-free Kit) to remove residual genomic DNA and then DNase I was inactivated with the recommended reagent.
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bioRxiv - Microbiology 2020Quote: ... 20 μg of bacterial RNA were treated with DNase I (Invitrogen™ Ambion™ TURBO DNA-free Kit) to remove residual genomic DNA and then DNase I was inactivated with the recommended reagent.
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bioRxiv - Microbiology 2021Quote: ... 2×104 iBMDMs (6-well plates) were transfected in suspension with 20 nM siRNA using Lipofectamine RNAiMAX (Invitrogen) in 200 μ Opti-MEM I (ThermoFisher).AllStars negative-control siRNA (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... 20μL of protein samples were run on 4-20% gradient tris-glycine gels (Invitrogen, Gand Island, NY, USA) for 120min at 130V and were transferred onto nitrocellulose membranes overnight at 20V ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 500 μl 50 mM Tris pH 7.5 with 10 μl 20 mg/ml Proteinase K (Invitrogen) and placed at 50 °C for 2 h ...
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bioRxiv - Cancer Biology 2021Quote: ... 20-25 μg of proteins were separated using 4-12% gradient NuPAGE Novex Bis-Tris gel (Life technologies). Protein transfer was performed on methanol-activated PVDF or Nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... 14 DIV cultures were incubated with the lipophilic dye CM-DiI (ThermoFisher C7000, 5 µg/ml, 20 min), followed by a PBS wash ...
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bioRxiv - Microbiology 2021Quote: ... The coverslips were stained with DAPI for 90 sec and mounted with 15-20 μl Antifade (Invitrogen, USA) to reduce photo-bleaching ...
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bioRxiv - Plant Biology 2020Quote: Reactions were carried out in a 20 μL volume using the Verso™ cDNA synthesis Kit (ThermoFisher Scientific). One reaction was prepared for each age cohort sample according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cryo-transmission electron microscopy (Cryo-TEM) was performed on an FEI Tecnai G² 20 system (ThermoFisher Scientific, USA) at an acceleration voltage of 200 kV ...