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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... Lysate controls were prepared by lysis of 5 OD cell pellets with 35 μL of YPER (Fisher Scientific, Waltham, MA, USA # 8990) at RT for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free plasma was analyzed using a human magnetic cytokine panel providing simultaneous measurement of 35 cytokines (Thermo Fisher Scientific, Waltham, MA). The assay was performed according to the manufacturer’s instructions with each subject sample performed in duplicate and then analyzed on a Luminex FLEXMAP 3D instrument.
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated on a microcapillary column (100 μm inner diameter) packed with ∼ 35 cm of Accucore150 resin (2.6 μm, 150 Å, Thermo Fisher Scientific) with 5-30% (3-108 min) ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were separated on a microcapillary column (100 μm inner diameter) packed with ∼ 35 cm of Accucore150 resin (2.6 μm, 150 Å, Thermo Fisher Scientific) with 8-23% (3-73 min) ...
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bioRxiv - Developmental Biology 2021Quote: ... total RNA was extracted from pools of 15 7-dpf larvae or 35 48-hpf embryos with TRIzol reagent (15596018, Thermo Fisher Scientific). mRNA was treated with RQ1 RNase-Free DNase (M6101 ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35-cm long Accucore150 resin (2.6 μm, 150 Å, Thermo Fisher Scientific). We loaded 4 μL onto the column and separation was achieved using a 180 min gradient of 8 to 23% acetonitrile in 0.125% formic acid at a flow rate of ~550 nL/min ...
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bioRxiv - Neuroscience 2021Quote: ... The brain was sectioned at 35 μm for anatomical tracing and 40 μm for other applications using a freezing microtome (HM 430; Thermo Fisher Scientific). Every third section was first Nissl stained (Thionin staining) ...
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bioRxiv - Plant Biology 2021Quote: The pCR8-ISR1 construct was used to subclone the gene into the pMDC32 (35S cauliflower mosaic virus [CaMV] promoter) or pEarleyGate103 (35S CaMV promoter with an N terminal GFP fusion) vectors using Gateway LR Clonase II (Thermo Fisher Scientific) and then stably expressed into isr1-1 and isr1-2 plants following Agrobacterium tumefaciens (strain GV301)-mediated transformation using the floral dip method (Clough and Bent ...
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bioRxiv - Molecular Biology 2022Quote: ... which was further increased to 35% followed by a rapid ramp-up to 85% using a Dionex Ultimate 3000 UHPLC (Thermo Fisher Scientific). The eluted peptides were then ionized via nanoelectrospray ionization ...
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bioRxiv - Genetics 2022Quote: ... Cells were transfected with the indicated siRNAs (15 pmol of total siRNA per 35-mm dish) (Baggs et al., 2009) using the Lipofectamine 3000 transfection reagent (Thermo Fisher # L3000) and according to the manufacturer’s instructions for 6-well plates ...
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bioRxiv - Immunology 2022Quote: ... Cells and DNA mix were resuspended in buffer ‘R’ before electroporation in 100 µl pipettes at 1350 volts for 35 ms in Neon™ electroporation system (Invitrogen; MPK5000). Cells were resuspended in mixed culture medium in a 6-well plate and allowed to recover for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... The coding sequences of YFP and BIN2 cloned in pENTR/SD/D-TOPO were subcloned into a Gateway-compatible 35S-YFP-TurboID by LR reaction (Invitrogen, Carlsbad, CA).
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bioRxiv - Neuroscience 2021Quote: ... Brain tissue was frozen in TissueTek and sectioned at 35 to 50 μm thickness on a cryostat (Thermo Scientific Microm HM 550) at −20° C ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells were cultured on 13 mm round coverslips in 35 mm dishes and transfected with pAAV/sCSF1R using Lipofectamine 2000 (Thermo Fisher Scientific). One day after transfection ...
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bioRxiv - Genomics 2020Quote: ... Nuclei and buffer from all wells were pooled in a reagent reservoir and passed through a 35 micron strainer into Corning Falcon test tubes (Thermo Fisher Scientific). DAPI was added and nuclei were sorted again with a BD FACS Aria III ...
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bioRxiv - Immunology 2020Quote: ... for 90min effective gradient with 4-35% 0.1% formic acid in ACN on an Ultimate 3000 Nano liquid chromatography instrument (Thermo Scientific, Waltham, MA). The separated peptides flew to an Orbitrap Q-Exactive HF mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: Mice were culled 35 days after initial infection and received an intraperitoneal injection (10ml/kg) of BrDU labelling reagent (no. 00-0103, Invitrogen, Camarillo CA) 40 minutes before culling ...
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bioRxiv - Cell Biology 2020Quote: ... mature organoids (day 35-45) were washed (PBS without Ca2+ and Mg2+) and embedded in 3 % low-melting-point agarose (Thermo Fisher Scientific) at 40 °C in reusable silicone molds (1 cm³) ...
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bioRxiv - Genetics 2022Quote: ... and immediately placed on a 0.4 micron Millicell cell culture inserts (PICMORG50) in a 35 mm dish with phenol-free DMEM (Thermo Fisher Scientific, 11880028) containing 5% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... to postnatal day 4 (P4) and placed in 35-mm Petri dishes containing Dulbecco’s phosphate-buffered saline (PBS; Thermo Fisher Scientific, 14190) on ice ...
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bioRxiv - Pathology 2024Quote: ... primary dermal fibroblasts (ATCC) detached via trypsinization were seeded (2 × 104 cells/mL) onto an EASY GRIP Tissue Culture Dish (35 mm; Thermo Fisher Scientific) and cultured in 10%FBS-DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto coated 35 mm glass bottom dishes with a 20 mm glass aperture (Fisher Scientific D35-20-1.5-N). HEK293A cells were seeded onto dishes that had been coated with 10 µg ECL cell attachment matrix (Sigma 08-110 ...
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were treated with 35 µL of Visikol® Histo-M™ in a regular 384-well plate (Thermofisher Scientific; 242757) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were strained through a 35 µm strainer and incubated at 37°C for 30 minutes with 0.5 µL Vybrant™ Dyecycle™ Ruby strain (Invitrogen, V10309) per mL of FANS buffer.
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bioRxiv - Bioengineering 2024Quote: ... the HLOs were treated with 35 µL of Visikol® Histo-M™ in a regular 384-well plate (ThermoFisher Scientific; 242757) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... with a 75 min gradient and Nano capillary column (100 µm D) packed with ∼35 cm of Accucore C18 resin (Thermo Fisher Scientific). A FAIMSPro (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The column was operated at a constant temperature of 35°C and the LC system coupled to a Q-Exactive mass spectrometer (Thermo Fisher Scientific). Chromatography was performed with a buffer system consisting of 0.1% formic acid (buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The column was operated at a constant temperature of 35°C and the LC system coupled to a Q-Exactive mass spectrometer (Thermo Fisher Scientific). Chromatography was performed with a buffer system consisting of 0.1 % formic acid (buffer A ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded onto 6-well plates or 35-mm dishes and transfected at 70–80% confluence with Lipofectamine RNAiMax (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: For constitutive secretion of anti-CD47 A4-Fc fusion proteins by murine T cells the cDNA sequence of the high-affinity anti-mouse CD47 nanobody A4 (35) was codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific), fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1 ...
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bioRxiv - Cell Biology 2022Quote: ... this mixture was added dropwise to confluent HEK-293T cells (ATCC, CRL-3216) in a T175 flask containing 35 mL DMEM media (Gibco #11965-092) and supplemented with 1mM sodium pyruvate (Gibco #11360-070) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and placed in 35 mm x 10 mm plastic petri dishes (Falcon # 351007) with bottle top-filtered (0.2 μm pore size; Thermo Scientific # 291-4520) natural sea water with streptomycin (2ug/mL ...
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bioRxiv - Immunology 2023Quote: ... Any remaining linear DNA was degraded by addition of 35 µL 10X Exonuclease I buffer and 20 µL Exonuclease I (ThermoFisher Scientific, 720735KU) and incubation at 37 °C for 2 h followed by 80 °C for 20 min ...
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bioRxiv - Immunology 2023Quote: EAE was induced in 10-12 week old mice by subcutaneous flank injection of 150 μg of myelin oligodendrocyte glycoprotein peptide (residues 35-55; MOG35-55) (Genemed Synthesis, Inc., San Francisco, USA) emulsified in incomplete Freund’s adjuvant (Thermo Fisher Scientific; DF0639606) supplemented with 5 mg/mL heat-inactivated Mycobacterium tuberculosis (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The column was operated at a constant temperature of 35°C and the LC system coupled to a Q- Exactive mass spectrometer (Thermo Fisher Scientific). Chromatography was performed with a buffer system consisting of 0.1 % formic acid (buffer A ...
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bioRxiv - Bioengineering 2023Quote: ... extension: 68 °C for 42 secs and total number of cycles: 35) in ProFlex 3×32-well PCR system (Applied Biosystems, USA). Desired amplified product was excised from 1.2% agarose gel and purified using GeneJET gel extraction and DNA cleanup kit following supplier’s protocol (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... mounted with a 100 μm capillary column that was packed with 35 cm of Accucore 150 resin (2.6 μm, 150 Å; Thermo Fisher Scientific). Peptides were separated at 525 nL/min flow rate using two buffers as mobile phases (Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were separated on a 100 μm inner diameter microcapillary column packed with 35 cm of Accucore C18 resin (2.6 μm, 100 Å, Thermo Fisher Scientific). Peptides were separated using a 3 hours gradient of 6–22% acetonitrile in 0.125% formic acid with a flow rate of ∼400 nL/min ...
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bioRxiv - Genomics 2023Quote: ... To achieve a sufficient level of chromatin digestion (50-75% of DNA in mononucleosomal fragments) we added 35-40U of MNase (Thermo Scientific, EN0181) and incubated the cells at 37°C with shaking for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were incubated at 37 °C for up to 35 minutes before adding four times the dissociation solution volume of quenching solution composed of DMEM/F12 (Thermo Fisher Scientific), 10% FBS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... for a 35 mm dish 4 μl of Lipofectamine 2000 and selected plasmids (3 μg) were diluted in Opti-MEM medium (Thermo Fisher Scientific) to a final volume of 250 μl and incubated for 20 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized using 35 to 200 ng of purified RNA using oligo-dT primers and M-MLV Reverse Transcriptase (Invitrogen, 28025-013). Real-time qPCR was performed using SYBRTM Green PCR Master Mix (Applied Biosciences ...
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bioRxiv - Molecular Biology 2024Quote: ... and immediately placed on a 0.4 micron Millicell cell culture inserts (PICMORG50) in a 35 mm dish with phenol-free DMEM (Thermo Fisher Scientific, 11880028) containing 5% FBS ...
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Aberrant regulation of serine metabolism drives extracellular vesicle release and cancer progressionbioRxiv - Cancer Biology 2024Quote: ... were seeded into 35 mm uncoated glass dishes (3910-135-MYP, IWAKI) and 4-well glass chamber slides (154526, Thermo Fisher Scientific). After 24 hours of incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... Nucleic acids and agar were degraded in the clarified extracted by adding benzonase (35 units/ml, Thermo Fisher Scientific, Cat. No. 88700) and agarase (0.2 units/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-mouse or anti-rat with conjugated fluorochromes (Alexa Fluor 488, 568 or 647; ThermoFisher) at 1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 488 goat anti-rat IgG (H + L, Thermo Fisher Scientific Cat# A-11006), CF488A goat anti-rat IgG (H + L ...