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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: R-follicles were incubated for 1 hour in Maintenance Media containing freshly prepared 2 µM Calcein-AM (C3100MP, Invitrogen). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in goat anti-rabbit Alexa Fluor 568 secondary antibody (1:200 in 2% BSA and 0.1% Triton; Invitrogen) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... The Dynabeads were washed three times with 1 ml of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific), with each wash performed after 1 minute of incubation ...
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bioRxiv - Microbiology 2024Quote: ... and the HIV-1 latently infected CD4+ CEM cells ACH-2 (A3.01 cell integrated HIV-1 proviral DNA) were cultured in complete RPIM640 (Gibco) containing 10% FBS and 1% penicillin/streptomycin at 37°C in an atmosphere of 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with serum free RPMI 1640 medium and incubated with 1 μM Rhod-2-AM (Invitrogen, R1244) together with antibodies for CD4 (GK1.5 ...
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bioRxiv - Cell Biology 2024Quote: ... for immunofluorescence analysis or were lysed in IGEPAL-C360 lysis buffer (20 mM Tris HCl pH 8.1, 37 mM NaCl, 1% IGEPAL-C360, 10% glycerol, 2 mM EDTA supplemented with protease inhibitor cocktail from ThermoFisher) for immunoblotting.
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bioRxiv - Cell Biology 2024Quote: ... About 1-2 μg indicated plasmid was used according to the instruction of lipofectamine 3000 (Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2024Quote: ... activated and unactivated iPSC-Tregs were co-cultured with activated (2:1 beads:cells) Tconv labeled with Cell Trace Violet (Thermofisher) in X-VIVO15 media supplemented with pen/strep ...
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bioRxiv - Cell Biology 2024Quote: ... and RT was performed with 1-2 μg RNA using SuperScript™ IV VILO™ Mastermix (11756050, Thermo Fisher) with the following conditions ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... slides were incubated for 1 h at RT in a humid chamber with secondary antibodies in PBS-T and 2% milk: 1:1,000 goat anti-mouse Alexa Fluor 488 (A11001; Invitrogen) and 1:1,000 donkey anti-rabbit Alexa Fluor 555 (A31572 ...
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bioRxiv - Cell Biology 2023Quote: Slides were rehydrated with dPBS for 2 minutes before incubation in 1:500 wisteria floribunda agglutinin (Invitrogen, Waltham, MA) overnight at room temperature in the dark in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% (v/v) sodium deoxycholate) supplemented with 1% protease inhibitor cocktail and 2% RiboLock RNase Inhibitor (#EO0381, Thermo Scientific). The RNA was sheared ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated with 100 μl of 2 mg ml-1 p-NPP (p-nitrophenyl phosphate) substrate (#34045; Thermo Fisher) diluted in AP-buffer ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... sections were washed and incubated for 2 h with secondary antibodies conjugated to Alexa-488 (1:2000; Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Human β-arrestin 2 (residues 1-393, with I386A, V387A, and F388A mutations) was cloned into pBiT2.1 vector (Invitrogen) with the SmBiT at its N-terminus ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNAs were synthesized from total RNA (1 μg) for each sample using SuperScript 2 reverse transcriptase (18064014, Thermo Fisher), diluted ten times with water ...
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bioRxiv - Molecular Biology 2023Quote: 1×105 HeLaK cells expressing GFP or GFP-ZNF512B or treated with Doxorubicin (100 nM, 2 days, Fisher Scientific) were seeded on coverslips in 24-well plates ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Genetics 2023Quote: ... PCR amplicons were resolved by 1–2% agarose gel electrophoresis and visualized by staining with SYBR Safe (Thermo Scientific). Negative control samples were always analyzed in parallel with experimental samples to identify mis-priming products ...
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bioRxiv - Cell Biology 2023Quote: ... for 5 min and hybridized with smFISH probes diluted 1:3000 in hybridization buffer (10% dextran sulfate, Sigma D8906, 20% formamide, 1 mg/ml E. coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 1:5000 dilution of Alexa Fluor 488 labeled goat anti-rabbit IgG 2° Ab (Invitrogen, A-11070) with blocking buffer and incubated for 30 min at 4°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... all applicable cell lines were selected using Puromycin (L929 – 10μg/mL, C3H – 1 ug/mL, NIH3T3 2 μg/mL, ThermoFisher) and Hygromycin B (L929 ...
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bioRxiv - Cancer Biology 2023Quote: ... THP-1 cells were cultured in R10 supplemented with 0.05 mM 2-mercaptoethanol (21985023; Thermo Fisher Scientific, Waltham, MA). MV-4-11 cells were cultured in IMDM supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... PANC-1 and MiaPaCa-2 were cultured and maintained in growth medium [DMEMF/12 with GlutaMAX™ Supplement (Gibco), + 10% v/v Heat Inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were subsequently incubated for 2 hours in secondary antibodies (all 1:500; Thermo Fisher Scientific, Waltham, MA, USA) diluted in 5% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... inoculation of 2×106 THP-1 cells expressing firefly luciferase and GFP in 100 μL of PBS (Gibco, USA). Mice were randomly assigned to 4 experimental groups and 1 control group ...
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bioRxiv - Microbiology 2023Quote: ... the sections were incubated with SARS-CoV-2 antibody against the nucleocapsid (N) protein (ThermoFisher MA536086; 1:28,000 dilution) for 15min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated with DMEM supplemented with 1% Pen/Strep and 2% (v/v) horse serum (Gibco, differentiation medium, DM) at the same condition for differentiation ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were cultured at a 1:2 cell:bead ratio with cultured αCD3/αCD28 activator beads (Thermo Fisher Scientific) and 50 ng/mL recombinant IL-2 (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... samples were incubated in Tyramide development solution (2% Dextran sulfate, 0.0015% hydrogen peroxide, 0.2mg/ml Iodophenol, 1:100 Alexa Fluor 488 Tyramide (ThermoFisher # B40953) in PTw ...
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bioRxiv - Bioengineering 2024Quote: ... PEGαMA hydrogels were made by dissolving the PEGαMA in pH 8.4 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Life Technologies) at 12.5-15.5 weight percent (wt%) ...
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bioRxiv - Microbiology 2024Quote: ... synchronized iRBCs at the trophozoite stage (5-10% parasitemia, 1% hematocrit) were resuspended in binding medium (RPMI 1640 medium, 2% glucose, pH 7.2; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... Cells were seeded on glass petri-bound hydrogels prepared with 1 µl/ml of 0.1% FluoSpheres Carboxylate-modified microspheres (0.2 µm, 580/605, 2%) (Thermo Fisher) aqueous solution ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by cell viability staining with LIVE/DEAD™ Fixable Blue/Violet (2 µl/ 1×106cells) (Invitrogen, ThermoFisher Scientific) for 20 minutes at room temperature in darkness ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with dH2O (1 time x 2 minutes) and mounted using Fluoromount-G (Thermo Fisher Scientific, #00-4958-02).
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bioRxiv - Cancer Biology 2024Quote: ... followed by cell viability staining with LIVE/DEAD™ Fixable Blue/Violet (2 µl/ 1×106cells) (Invitrogen, ThermoFisher Scientific) for 20 minutes at room temperature in darkness ...
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bioRxiv - Immunology 2024Quote: 2×106 THP-1 cells were centrifuged for 5 min at 1200 rpm and washed in 1X PBS (Gibco). They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated at room temperature for 2 hours in the secondary antibodies (Alexa 488 Donkey anti-Chicken, 1/200, Invitrogen; and CY3 Donkey anti-rabbit ...
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bioRxiv - Cell Biology 2024Quote: ... worms were immobilized on 2% agar pads on microscope slides in ∼1 μl of 100 mM levamisole (ThermoFisher #AC187870100) and then coverslip applied.
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bioRxiv - Immunology 2019Quote: ... and IL-2 concentration was measured using a mouse IL-2 ELISA kit (Invitrogen). For CFSE dilution assays ...
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bioRxiv - Microbiology 2020Quote: ... Candidate drugs were diluted 2-fold seriously by medium supplemented with 2% FBS (GIBCO), penicillin (100 IU/mL) ...
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bioRxiv - Microbiology 2021Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850); (3 ...
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bioRxiv - Immunology 2020Quote: ... Washing medium consisted of 2% FCS and 2% P/S in RPMI 1640 (Gibco). FACS buffer contained 2% FCS and 2mM EDTA in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg/ml goat anti-rabbit F(ab’)2 fragment (Life Technologies A-21246) and goat anti-mouse F(ab’)2 fragment secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed three times with 2× saline sodium citrate (2×SSC, ThermoFisher AM9765), and equilibrated with encoding-probe wash buffer (30% formamide (ThermoFisher AM9342 ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.4) supplemented with 2 μM fura-2-AM (F1201, Molecular Probes, Eugene, OR) and 0.01 % pluronic acid ...