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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 2 μM Fura-2 AM (Thermo Fisher Scientific, F1225) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mM glutamine and 2 mM HEPES (Thermo Fisher Scientific, Waltham, MA, USA). Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco 15630080) and 1 % penicillin-streptomycin (PS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10% glycerol (2 mM 2-mercaptoethanol (BME) and protease inhibitor tablet (ThermoFisher Scientific) added immediately before use) ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... They were then maintained in RPE medium (70% DMEM, 30% Ham’s F12 nutrient mix, 2% B27-serum-free supplement, 1% antibiotic-antimycotic solution (Invitrogen)) for 2-3 months to form mature RPE monolayers ...
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bioRxiv - Cell Biology 2020Quote: ... the suspension was diluted 1:2 with fresh medium and passed through a 40 μm cell strainer (Fisher Scientific) to obtain single cell suspension ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (ARGi ...
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bioRxiv - Immunology 2021Quote: ... Proliferation was triggered by the stimulation with Dynabeads Human T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific). The arginase inhibitor OAT-1746 (1500 nM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Nickase-1/2 constructs were individually transfected into CH12 via electroporation with Neon Transfection System (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: ... diluted (1:10,000) in pre-incubation (PI) buffer (PBS, 0.1% Tween 20, 2% sheep serum, 2mg/ml BSA (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed with PBS followed by staining using streptavidin 488 (1:300, Jackson) diluted in PBS containing 0.4% Triton-X and 2% normal horse serum (NHS; Gibco) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... secondary Ab was incubated for 2 hours at room temperature (Alexa568-conjugated anti-mouse Ab, 1:400, Life Technologies). Brains were then mounted in Vectashield (Vector Labs ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (∼1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
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bioRxiv - Molecular Biology 2020Quote: The remaining RNA was combined into four final bsRNA-seq fractions (Figure 1) and 10μg RNA was spiked with ERCC Spike-in Mix 2 (Ambion) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... whole menisci were cut into small pieces (1-2 mm) and digested in DMEM (11996-065, Gibco, Carlsbad, CA) containing 2% penicillin/streptomycin (15140-122 ...
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bioRxiv - Genomics 2020Quote: ... Beads were handled before use with RNase OUT (40 U/μL; Life Technologies; 2 U enzyme/1 μL bead). The dRNA library was run on a R9.4 SpotON Flow Cell ...
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bioRxiv - Microbiology 2019Quote: Monoclonal antibodies (mAbs) were serially diluted 1:2 across white 96-well Nunclon© plates (Thermo Fisher Scientific, UK) in 50 μl of DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... and Alexa Fluor 488 F (ab’)2-Goat anti-Mouse IgG (Thermo Fisher Scientific, A-11017; diluted 1:500). Slides were mounted with Fluoroshield (Sigma ...
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bioRxiv - Immunology 2019Quote: ... Germany) and transfected into Expi293 cells grown in a 1:2 mixture of Expi293 and Freestyle (Gibco, Thermofisher Scientific) media ...
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bioRxiv - Immunology 2019Quote: ... Germany) and transfected into Expi293 cells grown in a 1:2 mixture of Expi293 and Freestyle (Gibco, Thermofisher Scientific) media ...
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bioRxiv - Neuroscience 2019Quote: ... LUHMES neuroblastoma cells (ATCC Cat# CRL-2927) were maintained DMEM/F12 (ATCC) supplemented with N-2 supplement (1%; Invitrogen) and human recombinant basic fibroblast growth factor (40 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... using different combinations (ratio 1:1.5:2) at 70-80 % confluency using reverse transfection by Lipofectamine 2000 (ThermoFisher Scientific), as indicated ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Cell Biology 2020Quote: ... Gel pieces were incubated in Elution buffer (1% formic acid, 2% acetonitrile in LC-MS grade water (Thermo Scientific)) and dried in a SpeedVac ...
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bioRxiv - Neuroscience 2019Quote: ... Thiol reactive fluorescent probe IAEDANs (1,5-IAEDANS, 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic Acid) was purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... was then applied for 2 hours along with an Alexa Flour 546 anti rabbit antibody (Life technologies, 1:500). After five washes ...
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bioRxiv - Cancer Biology 2019Quote: The selected AsPC-1 and Capan-2 PC cell lines were transfected with the GFP-LC3 lentiviral vector (Invitrogen). Transfection flow cytometry analysis was then conducted to isolate cell populations showing stable expression of GFP-LC3.
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bioRxiv - Developmental Biology 2019Quote: ... 0.25 μM gene-specific primers (Extended Data Table 2) and 1 x Power SYBR Green Master Mix (ThermoFisher Scientific) in a StepOne Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2021Quote: ... RNA contents were isolated from the cells 1 or 2 hours after stimulation using TRIzol Reagent (Thermo Fisher Scientific) and reverse-transcribed using high-capacity cDNA reverse transcription kit (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2021Quote: ... A 2 μl aliquot of a 1:10 dilution of the ERCC RNA Spike-in Mix (Thermo Fisher Scientific) were added to each sample ...
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bioRxiv - Developmental Biology 2021Quote: Two BCAM-targeting siRNAs (siBCAM #1, siBCAM #2; Dharmacon) were transfected into CT29 hTSCs using Lipofectamine RNAiMAX (Thermo Fisher) at a concentration of 100 nM ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 555-conjugated F(ab’)2 fragment donkey anti-rabbit (1:500; A31572, Invitrogen, Thermo Fisher Scientific, Waltham, MA), AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... cells were live-dead stained with 2 µM calcein acetoxymethyl and 4 µM ethidium homodimer-1 (#L3224, Thermo Fisher) and imaged using fluorescent microscopy to compare cell density and viability between each silicone substrate ...
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bioRxiv - Biochemistry 2021Quote: ... 2 µg PAX2 and 4 µg shRNA in pLKO.1 backbone using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy3-labeled species-specific secondary antibodies for 2 h at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch). To avoid cross talk between multiple fluorophores ...
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bioRxiv - Neuroscience 2020Quote: ... before 2-h incubation at room temperature with a secondary antibody Alexa 555 made in rabbit (Invitrogen, 1:500). After several washes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Systems Biology 2021Quote: We isolated pre-adipocyte cells from subcutaneous adipose tissue by dissecting tissue into small (1-2 mm) pieces with scissors and performing a 0.1% (w/v) collagenase II (Gibco) digestion in DMEM at 37oC for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Each 20-μlL PCR mixture contained 2 μL of 10X Accuprime PCR buffer II (Life Technologies, CA, 1 USA), 0.15 μL of Accuprime high-fidelity polymerase (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then run on a Native PAGE gel and visualized with Alcian Blue (1:20 dilution of 1.25% stock solution in 2% acetic acid) followed by Silver Staining (ThermoFisher) following manufacturer protocols and imaged on the Bio-Rad Chemidoc MP Imaging System.
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bioRxiv - Microbiology 2021Quote: ... 2.5 mM probenecid were added to the cultures which were diluted 1:2 in DMEM with GlutaMAX (Gibco, Germany). 50 µl of the bacterial suspension were subsequently added to the screening plates which contained 1 µl of compound per well (concentrations are given in Table 1) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 and 3 dpf embryos were injected with 1–2 nL dextran tetramethyl rhodamine or dextran fluorescein (molecular weight 2,000 kDa, Invitrogen) at 10 mg/mL and imaged immediately ...