Labshake search
Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 6 Benzothiazolamine 2 1 methylpropyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2021Quote: ... 4T1 cells or Py8119 cells suspended at 1-2 million/150 µl of 33% (v/v) matrigel (Fisher Scientific) were transplanted subcutaneously to the right flank of 6-8 week-old BALB/c or C57BL/6J female mice ...
-
bioRxiv - Cell Biology 2022Quote: ... iPSCs were transfected with PB-TO-hNGN2 vector (gift from M. Ward, NIH, Maryland) in a 1:2 ratio (transposase:vector) using Lipofectamine Stem (ThermoFisher); after 72 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... and then washed with 10% formamide in 2× SSC solution with Hoechst 33342 (1:1000, Thermo Fisher Scientific, H3570) and incubated at 37°C for 30 min in the dark ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µl of 2 µM Uni9 primer and 1µl of 10 mM dNTP (Thermo Scientific™, Cat no. # R0181) were mixed and warmed at 65 ºC for 5 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
-
bioRxiv - Microbiology 2021Quote: ... bacteria were washed by centrifugation three times (11000 rcf for 2 minutes) and resuspended to OD 1.0 (~1 × 109 bacteria/ml) in RPMI (Gibco) + 0.05% human serum albumin (HSA ...
-
Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Cells were electroporated with 2 pulses at 65 mV for 20 ms in a 1 mm cuvette (ThermoFisher, #15437270). After electroporation ...
-
bioRxiv - Cell Biology 2021Quote: ... The coverslips were 1-or 2-days post-transfection mounted in an AttoFluor cell chamber (A7816, Thermo Fisher Scientific) and microscopy medium (137 mM NaCl ...
-
bioRxiv - Neuroscience 2021Quote: A solution of 2 μM of Calcein-AM and 8 μM of ethidium homodimer-1 (both from L3224, ThermoFisher) in DPBS (14190-144 ...
-
bioRxiv - Neuroscience 2022Quote: ... The tissue powder was transferred to 2 mL microcentrifuge tube and 1 mL TRIzol Reagent (ThermoFisher, Cat no 15596018) was added ...
-
bioRxiv - Systems Biology 2022Quote: ... Cells were split 1-2 times per week after dissociation with EDTA in DPBS (final concentration 0.5mM) (Gibco, 12605010). Media was supplemented with Rho-associated protein kinase (ROCK ...
-
bioRxiv - Immunology 2022Quote: ... Biotinylated-SARS-CoV-2 tri-S proteins were mixed with FITC-labelled NeutrAvidin beads (1 µm, Thermo Fisher Scientific) (1 µg of tri-S for 1 µl of beads) ...
-
bioRxiv - Immunology 2022Quote: ... 1.5×106 CD45.1 cells were incubated with 2×106 Balb/c splenocytes in the presence of 1 mg/mL GolgiStop (Invitrogen) for 4-5 h at 37 C ...
-
bioRxiv - Neuroscience 2019Quote: ... Slices were then incubated for 2 hours with the Alexa Fluor 488 secondary antibody (Invitrogen, Carlsbad, CA, 1:500) and counterstained with Neurotracer fluorescent Nissl (Invitrogen ...
-
bioRxiv - Developmental Biology 2020Quote: ... cDNA was synthesized using a 2:1 ratio of random primers to Oligo(dT) with SuperScript IV RT (Invitrogen). qPCR was performed with PerfeCTa SYBR Green FastMix for iQ (Quanta Biosciences ...
-
bioRxiv - Biophysics 2019Quote: ... After rinsing with a solution containing NPE-caged ATP (adenosine 5′-triphosphate, P3-(1-(2-nitrophenyl) ethyl) ester) (Invitrogen) (0 to 1 µM ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The cells were transiently transfected with 1 µg plasmid DNA with 2 µL of Lipofectamine 2000 (Thermo Fisher Scientific). 24-hours after transient transfection ...
-
bioRxiv - Immunology 2019Quote: For activation of MAIT cells by bacteria-derived ligands, THP-1 cells were loaded with PFA-fixed (2%, 20min) E.coli (DH5α, Invitrogen) at a 25 bacteria per cell (BpC ...
-
bioRxiv - Genetics 2021Quote: ... 20 μl reaction mix with 2 μl cDNA (~1-10 ng) were monitored on StepOnePlus Thermal Cycler (Applied Biosystems) in “fast mode” ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... potential was examined using 5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1propenyl]-1,3-diethyl-,iodide (JC-1 dye) (Life Technologies, USA) as a probe ...
-
bioRxiv - Biochemistry 2019Quote: ... with the sole exception of β-mercaptoethanol being replaced by 1 mM Tris(2-carboxyethyl)phosphine (TCEP, Thermo Fisher). Cell disruption ...
-
bioRxiv - Genomics 2021Quote: ... The extracted RNA (1-2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was carried out in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
-
bioRxiv - Immunology 2020Quote: ... VH and VL plasmids were mixed at 1:2 ratio and were transfected into Expi293F cells (Thermo Fisher Scientific), which were cultured at 37 °C and 8% CO2 for 5 days ...
-
bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
-
bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
-
bioRxiv - Immunology 2021Quote: ... Copies of SARS-CoV-2 N were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
-
bioRxiv - Microbiology 2021Quote: ... followed by 2 h staining with secondary donkey anti-mouse Alexa Fluor 546 antibody (Invitrogen, A10036, 1:500 dilution) and 1μg/ml DAPI.
-
bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
-
Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... bacteria were washed by centrifugation three times (11000 rcf for 2 minutes) and resuspended to OD 1.0 (~1 × 109 bacteria/ml) in RPMI (Gibco) + 0.05% human serum albumin (HSA ...
-
bioRxiv - Microbiology 2019Quote: ... Cells were then washed extensively and media replaced with PBS containing 2 μgμL-1 FITC-conjugated epidermal growth factor (EGF, Invitrogen) for 30 min ...
-
bioRxiv - Developmental Biology 2021Quote: ... resuspendend in 1 mL M9 before 5 µL of DiI stock solution (2 mg/mL DiI (Molecular Probes, #D282) in dimethyl formamide ...
-
bioRxiv - Immunology 2021Quote: ... The sections were then incubated for 2 hours at room temperature using: rabbit polyclonal antibody-1 for myeloperoxidase (Invitrogen) to examine neutrophils ...
-
bioRxiv - Bioengineering 2022Quote: ... Half of the medium was replaced by pre-warmed PBS containing 2 μL Hoechst (1 mg/mL Thermofisher Scientific). After 30 min incubation ...
-
bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040 ...
-
bioRxiv - Cancer Biology 2022Quote: ... thinly sliced (1-2 mm) tissue samples were fixed overnight at 4°C in neutral-buffered formalin (Fisher Scientific) with PhosSTOP added (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... the mice were perfused for 1 min to 2 min with Hank’s balanced salt solution (14025-050; ThermoFisher Scientific), followed by 5 min perfusion with 4% paraformaldehyde (PFA ...
-
bioRxiv - Cell Biology 2022Quote: ... was transfected into Lenti-x 293T cells with 2:1 (psPAX2/pMD2G) plasmids using Lipofectamine 2000 (Thermo Fisher # 12566014) at a ratio of 1:1 (Lipofectamine 2000/nucleic acid (μg) ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were trypsinized and plated onto coverslips (1:2 split) or Nunc 8 well chamber slides (Thermo Fisher # 155409PK) (20-40k cells per well).
-
Alpha-1-antitrypsin binds to the glucocorticoid receptor with biological significance in macrophagesbioRxiv - Immunology 2022Quote: Human THP-1 cells were cultured in RPMI-1640 medium containing 2 mM L-glutamine (Gibco; Grand Island, NY), 10% FBS ...
-
bioRxiv - Molecular Biology 2022Quote: Drosophila Schneider 2 (S2) lines were maintained in 1 X Schneider’s medium supplemented with 10% heat inactivated fetal bovine serum (Gibco) and 50 mg/ml gentamicin antibiotic (Gibco) ...
-
bioRxiv - Neuroscience 2022Quote: ... Beads were resuspended in 400 μl NET-2 and extracted with an equal volume of phenol:chloroform:isoamyl 25:24:1 (Invitrogen 15593031). After centrifugation at 16,000 x g for 15 minutes ...
-
bioRxiv - Plant Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with the Ca2+ Calibration Kit #1 (Invitrogen, Waltham, USA).
-
bioRxiv - Cell Biology 2022Quote: ... The PAA gel surface was then incubated with a solution of 2 mg ml−1 Sulpho-SANPAH (4822589, ThermoFisher) under ultraviolet light for 5 min (wavelength of 365 nm at 5 cm distance) ...
-
bioRxiv - Plant Biology 2022Quote: ... 1 mM 2-mercaptoethanol and 10 mM imidazole) and then loaded onto a HisTrap HP column (ThermoFisher, Waltham, USA). The eluted fractions containing FHS-OGOX1 were pooled and dialyzed in 50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Neuroscience 2024Quote: ... Approximately 1 μg of RNA per sample (10.6 µL) was incubated with 2 µL TURBO DNase (Thermo Fisher Scientific) and 1.4 µL TURBO DNase buffer (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were dissociated and plated (1:2 ratio) at high density and maintained in maturation media (Neurobasal media (Gibco) + B27 supplement (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... sonicated in 1-2% SDS at 37 kHz and 100% power in a Fisherbrand™ bath sonicator (Fisher Scientific) for 10 minutes ...
-
bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
-
bioRxiv - Developmental Biology 2024Quote: ... Larvae were then kept at 28.5°C with fresh E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics). Around 3-4 hours after heat-shock ...