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Citations for Thermo Fisher :
4151 - 4200 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Digested peptides were analyzed by online two-dimensional liquid chromatography tandem mass spectrometry on an Orbitrap-Elite mass spectrometer (ThermoFisher Scientific). Mass spectra data were acquired in data dependent mode and the top ten most abundant parent ions were selected for further fragmentation by collision-induced dissociation at 35% energy level ...
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bioRxiv - Microbiology 2021Quote: ... Peptides of tryptic digested samples were separated over 2h on C18 material using an Ultimate3000 rapid separation system (Thermo Fisher Scientific) as described61 and subsequently analyzed with an online coupled mass spectrometer in data dependent mode ...
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bioRxiv - Neuroscience 2021Quote: ... Desalted peptides (300-500 ng) were injected onto an EASY-Spray PepMap RSLC C18 50 cm x 75 μm column (Thermo Scientific), which was coupled to the Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2021Quote: TMT-labeled peptides originating from the native oxidation and SPROX methods were injected onto an Easy nLC-1200 HPLC instrument (Thermo Fisher) using a 30 cm C18 column packed with 1.8 μm beads (Sepax) ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified DNA was used to generate mRNA-peptide fusions using in vitro transcription using retic lysate IVT Kit (Fisher Scientific), ligation of a puromycin hybridization and inter-calation linker (Keck Oligo Synthesis Lab ...
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bioRxiv - Biochemistry 2021Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 and LTQ-Orbitrap Velos Pro, Thermo Scientific). Peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn and separated on a 75 μm × 250 mm C18 column (PepMap ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were first trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap™ 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Biochemistry 2021Quote: ... Then the peptides were separated with high-performance liquid chromatography (HPLC, Ultimate 3000 Nano LC System, Thermo Scientific, Rockwell, IL, USA) in a 15-cm long C18 column (Acclaim® PepMap™ RSLC ...
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bioRxiv - Biochemistry 2021Quote: Multiple reaction monitoring (MRM) assay configuration using heavy labeled synthetic peptides was done on TSQ Quantiva triple quadrupole mass spectrometer (Thermo Fisher) coupled with Easy-nLC 1200 ultra-high pressure liquid chromatography (UPLC ...
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bioRxiv - Biochemistry 2020Quote: Peptides were separated using C18 chromatography and detected using a Q-Exactive HF-X Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific) in positive ion polarity ...
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bioRxiv - Biochemistry 2020Quote: ... purified peptides were measured using LC-MS instrumentation consisting of an EASY-nLC 1200 system (Thermo Fisher Scientific, San Jose, CA) interfaced on-line with a Q Exactive HF-X Orbitrap (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Tryptic peptides were analyzed by an automated LC–ESI–MS/MS consisting of an EASY-nLC system (Thermo Scientific, Bremen, Germany) on-line coupled to a Q Exactive mass spectrometer (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Sample aliquots comprising 25 μg of peptides were labelled with isobaric tandem mass tags (TMT 10-plex, Thermo Fisher Scientific, #90110) as described previously (31) ...
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bioRxiv - Neuroscience 2020Quote: ... Peptides were loaded on to 2cm trap column (C18, 5 μm, 100 Å, 100 μm × 2 cm; PN: 164562, Thermo Scientific) and further loaded on 50 cm Easynano spray analytical column (2 μm ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by peptide analysis of the digested gel bands by C18 reversed-phase chromatography using an UltiMate 3000 RSLCnano System (Thermo Scientific) equipped with an Acclaim PepMap C18 column (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Peptides were then separated and analyzed on an Easy-nLC 1000 system coupled to a Q Exactive HF (both - Thermo Scientific). About 2 µg of peptides were separated in an home-made column (75 µm x 15 cm ...
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S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... Peptides were loaded onto a PepMap RSLC C18 column (2 μm, 100 Å, 75 μm i.d. x 250 mm, Thermo Scientific) using a flow rate of 300 nL/min for 15 min at 1% B (mobile phase A was 0.1% formic acid in water and mobile phase B was 0.1 % formic acid in acetonitrile ...
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bioRxiv - Plant Biology 2021Quote: ... the purified protein was mixed with the appropriate concentration of each auxin before passing it over a streptavidin chip loaded with biotinylated SoIAA2 and SoIAA2Δ9 degron peptides (Thermo Fisher Scientific) (Supplementary Table 3).
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were analysed using a nanoflow liquid chromatography (LC-MS/MS) on an EASY-nLC 1000 system (Thermo Fisher Scientific) coupled to a Q Exactive HF-X Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Approximately 1 μg of peptides were desalted on a trap column (Acclaim PepMap100 C18, 5 μm, 100 Å, 300 μm i.d. × 5 mm, Thermo Scientific) and then separated on an in-house packed column (75 μm i.d ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of the peptide solution were chromatographed on a nano-capillary reverse phase column (Acclaim PepMap C18, 2 micron, 50 cm, Thermo Scientific) using a 0.1% formic acid/ 2% acetonitrile (Buffer A ...
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bioRxiv - Biochemistry 2020Quote: Individual fractions of the tryptic peptides were separated by a capillary C18 reverse phase column of the mass-spectrometer-linked Ultimate 3000 HPLC system (Thermo Fisher) using a 90 min gradient (5%-85% acetonitrile in water ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific), before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 10 μL 50 mM HEPES pH 8.5 and quantified by Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific 23275).
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bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then fractionated into 8 fractions using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific 84868), then dried by speed-vac ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was purified from the immunized rabbit sera using affinity purification against the same peptide coupled to SulfoLink resin (Thermo-Fisher Scientific). The antibody was dialyzed into PBS+ 50% glycerol and stored with the addition of 0.05% sodium azide.
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bioRxiv - Cell Biology 2021Quote: ... Peptides were directly ionized using a nanospray ion source and analyzed on the Q-Exactive HF mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... tryptic peptides were injected into an Easy-nLC 100 HPLC system coupled to an Orbitrap Fusion Lumosmass spectrometer (Thermo Fisher Scientific). Specifically ...
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bioRxiv - Cell Biology 2021Quote: ... with reverse phase chromatography to separate peptides using an Ultimate 3000 RSLCnano system (Dionex) before analysis on a Thermo Orbitrap Fusion (Q-OT-qIT, Thermo Scientific). RAW files from LC-MS/MS were processed using MaxQuant version 1.5.3.8 or 1.6.7.0 (http://www.maxquant.org ...
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bioRxiv - Plant Biology 2020Quote: Synthetic Arabidopsis RALF1 polypeptide was synthesized by using 9-fluorenylmethyl chloroformate solid-phase chemistry with a peptide synthesizer from Thermo Scientific company and confirmed by MALD-TOF analysis (Applied Biosystems Voyager System 2098 ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap C18, 5 μm, 100 μm × 2 cm, Thermo Fisher Scientific) connected to an analytical column (Acclaim PepMan 100 ...
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bioRxiv - Plant Biology 2020Quote: ... Eluted peptides were transferred to an NSI source and sprayed into an Orbitrap Q-Exactive Plus mass spectrometer (Thermo Fisher Scientific). The MS was run in positive ion mode ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Systems Biology 2021Quote: ... Peptides were resolved on analytical column (75 μm × 50 cm, 3 μm particle, and 100 Å pore size; Thermo Fisher Scientific) at a flow rate of 300 nl/min over 120 min gradient in solvent B (80% Acetonitrile with 0.1% Formic acid (FA) ...
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bioRxiv - Microbiology 2020Quote: ... The specific modification of phosphor-Thr and phosphor-Tyr residues in the treated peptide was measured by tandem mass spectrometry (Thermo Scientific™ Orbitrap Fusion™ ...
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bioRxiv - Systems Biology 2021Quote: ... Dried peptides were resuspended in MS loading buffer (3% ACN, 0.1 % FA) and measured with Q Exactive HF (Thermo Fisher Scientific) coupled to a reverse-phase nano liquid chromatography ACQUITY UPLC M-Class system (Waters) ...
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bioRxiv - Systems Biology 2020Quote: ... of pTyr peptides were carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher). Peptides were separated using a 140 min gradient with 70% acetonitrile in 0.2 M acetic acid at flow rate of 0.2 mL/min with approximate split flow at 20 nL/min ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μg peptides from each fraction was loaded onto trap column nanoViper 2 cm (3 μm C18 Aq) (Thermo Fisher Scientific). Peptide separation was carried out using EASY-Spray C18 analytical column (15 cm ...
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bioRxiv - Plant Biology 2021Quote: ... extracted peptides were separated on an EasyLC nano-HPLC coupled to a Q Exactive HF mass spectrometer (both Thermo Fisher Scientific) as described elsewhere (Kelstrup et al. ...
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bioRxiv - Microbiology 2021Quote: ... Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). Peptides were sampled on a 300 µm x 5 mm PepMap C18 precolumn and separated on a 75 µm x 250 mm C18 column (Reprosil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2021Quote: The LC-MS/MS analysis of TMT-labelled peptides were performed on an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) coupled with a Thermo Scientific Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitated proteins were eluted from beads with 0.2 ml PDE storage buffer containing 1 mg/ml HA-peptide (Thermo Fisher Scientific) with mixing at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... The application of a 3.5 kV distal voltage electrosprayed the eluting peptides directly into a Q Exactive HF mass spectrometer equipped with an EASY-Spray source (Thermo Scientific). Mass spectrometer-scanning functions and HPLC gradients were controlled by the Xcalibur data system (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... both preceded by a murine IgK leader peptide and followed by an 8xHis Tag were synthetized and cloned into pcDNA™3.1/Zeo(+) expression vector (Thermo Fisher). Proteins were produced by transient co-transfection of exponentially growing Freestyle™ 293-F suspension cells (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap MS (Thermo Scientific, San Jose, CA) for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization ...
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bioRxiv - Plant Biology 2021Quote: ... the peptides were dissolved in 4 μL of 0.2% formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15 cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Plant Biology 2021Quote: ... Total peptide samples and the immuno-enriched fractions were then analyzed via LC-MS/MS using an EASY-nLC 1200 (Thermo Fisher) coupled to a Q Exactive HF mass spectrometer (Thermo Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... The lyophilized peptide was dissolved in nuclease-free water and its concentration determined on a Nanodrop 2000c (Thermo Scientific, Waltham, MA) using A280 with the molar extinction coefficient (3230 M-1cm-1 ...