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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: (D1)2 and iE5 concentrations were estimated by UV absorption using NanoDrop™ One/OneC (Thermo Scientific) (extinction coefficients ...
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bioRxiv - Plant Biology 2024Quote: ... and the SuperScript III Platinum SYBR Green One-Step qRT-PCR Kit with ROX (Invitrogen Corp., USA). The qPCR was run using an ABI 7900DNA Sequence detector (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted under reducing conditions in the dark and quantified by nanodrop (NanoDrop One, Thermo Scientific). 5 μg of RNA was then treated with iodoacetamide and incubated at 50°C for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... followed by one hour incubation with donkey anti-mouse IgG [H+L]/ AF 550 (SA5-10167, Invitrogen) and one hour incubation with mouse anti-Beta-Amyloid MOAB-2/AF 750 (NBP2-13075AF750 ...
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bioRxiv - Immunology 2024Quote: ... Step one: wells were blocked for 1 hour in 80uL of 5% Blocker BLOTTO (ThermoFisher Scientific, #37530). Step two ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed on a Step One Plus Real-Time PCR System (Thermo Fisher Cat#43765592R) with QuantiTect SYBR Green PCR Kit (QIAGEN Cat#204145) ...
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bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 cells were fixed with 4% paraformaldehyde and then stored at 4°C in RPMI 1640 (Gibco, Cat# 11875119) media supplemented with 10% heat inactivated FBS ...
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bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... post-fixed in 4% paraformaldehyde and 30% sucrose for 4 days before being frozen in Neg50 OCT (Thermo Scientific) 30 micron sections were taken and stored in PBS with sodium azide at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Stock BODIPY505/515 was prepared by dissolving 4,-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene (Life Technologies, D3921) powder into dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed using a 4% PFA solution [0.2M sucrose | 4% paraformaldehyde solution in PBS (Thermo Fisher, J19943-K2)] ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
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bioRxiv - Cell Biology 2019Quote: ... OCT4 and TRA1-60 overnight at 4°C using the pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Life Technologies), followed by incubation with an ALEXA secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system ...
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bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... were treated either with 0.8 μM CF-4 or control CF-4 (Ctrl-S2-CF4) in DMEM without phenol red (Gibco) and incubated for 10 min at 37°C and 5% CO2 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1,000xg for 4 min and fixed with 4% PFA (Thermo Fisher, Cat. No. 28908) for 15 min on ice ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were run on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher), and data were analyzed using the comparative CT method to generate expression fold-change values ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were transiently transfected in 6-well plates with Lipofectamine 2000 (Life Technologies), as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 6-ketocholestanol or phloretin cells were incubated with di-8-ANEPPS (Thermo Fisher, D3167) at a final concentration of 2 μM on ice for 20 minutes (33 ...
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bioRxiv - Cell Biology 2020Quote: ... differentiation was initiated on day -6 by adding RPMI/B27 minus insulin (Gibco, USA) supplemented with 1 μM CHIR 98014 (Selleckchem ...
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bioRxiv - Microbiology 2021Quote: ... loaded on to a Novex Pre-cast 6% TBE-Urea (8M) polyacrylamide gel (Thermofisher) in 1X TBE and run for 45-60 min ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was suspended in urea Dye and resolved on 6% TBU gel (ThermoFisher Scientific) at 200 V for 5 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... Lower jaw timelapse experiments were carried out on 6-well transwell membranes (Nunc, 140642) with 2 ml of DMEM ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were anchored with succinimidyl ester of 6-((Acryloyl)amino) hexanoic acid (AcX) (Thermofisher) in PBS (0.1mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... IL-6 ELISA assay was performed on collected media (Affymetrix eBioscience, #88-7066-22).
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using QuantiStudio 6 (Life Tech) with SYBR Power Green (Applied Biosystems). All the primer sequences used in this study can be found in Table S1.
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bioRxiv - Molecular Biology 2021Quote: ... re-suspended in 6 μl water and quantified using Quant-IT RiboGreen (ThermoFisher, R11490). 1 μg RNA was resolved per lane on 1.2% glyoxal agarose gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 or 24-well dishes were transfected with siRNA using Lipofectamine RNAiMax (Life Technologies) following the manufacturers’ guidelines ...
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bioRxiv - Cell Biology 2022Quote: Cell transfections were performed in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... buffer at pH 6 and two jars with nuclease-free water (ThermoFisher Scientific, USA). The slide rack was fully immersed in hematoxylin for 6 minutes and then washed by dipping the slide rack in a nuclease-free water jar 5 times following another destaining wash by dipping the slide rack in 800mL nuclease-free water for 30 times ...
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bioRxiv - Genomics 2020Quote: ... DNA-protein complexes were separated on non-denaturing 6% DNA retardation gels (Invitrogen, #EC63655BOX) at 100V constant voltage for 50 min ...
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bioRxiv - Immunology 2021Quote: ... on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems; Foster City, CA). The primers and probe were specific to the SARS-CoV-2 nucleocapsid gene ...
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bioRxiv - Genetics 2019Quote: Genotype information was available for 21,001 NTR participants from 6 different genotyping arrays (Affymetrix 6.0 [N = 8,640] ...
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bioRxiv - Molecular Biology 2021Quote: NALM-6 cells were obtained from ATCC and cultured in RPMI medium (Life Technologies) with 10% foetal bovine serum (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... Transfections were carried out in 6-well Nunclon© plates (Thermo Fisher Scientific, UK).