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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A rapid, highly sensitive and open-access SARS-CoV-2 detection assay for laboratory and home testingbioRxiv - Molecular Biology 2021Quote: ... 1-step RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermofisher) or Luna Universal One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... A one-step RT-qPCR reaction was performed in a StepOnePlus Real-Time PCR System (Applied Biosystems). Cutoff points for Cq values (Cycle of quantification ...
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bioRxiv - Microbiology 2020Quote: ... All qRT-PCR was completed using the Superscript III platinum One step qRT-PCR kit (Life Technologies) following manufacturer’s instructions for reaction set up ...
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bioRxiv - Neuroscience 2021Quote: ... pcDNA3.1-Ptprt was cloned from mouse brain RNA by SuperScript III one-step RT–PCR system (Invitrogen). Lentiviral shRNAs against mouse ptprt were constructed in pLKO.1 (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The one-step RT-qPCR was run using a qPCR system (QuantStudio 3, Thermo Fisher Scientific, USA) with the following thermocycle ...
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bioRxiv - Systems Biology 2021Quote: ... This barcoded destination vector pool was transformed into One Shot ccdB Survival 2 T1R Competent Cells (Invitrogen) that were spread on 245 mm × 245 mm square LB+ampicillin plates and incubated overnight at 37°C for colony isolation ...
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bioRxiv - Pathology 2021Quote: ... One milliliter of blood was harvested in a 2mL tube containing 100µL of 0.5M EDTA (Life Technologies). Blood was centrifuged for 10 min at 16,200g and stored at -80°C.
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bioRxiv - Cell Biology 2021Quote: ... One microgram of total RNA was reverse transcribed with random hexamers using Superscript II reverse transcriptase (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... The In-Fusion cloning product was again transformed into One Shot Stbl3 Chemically Competent E.coli (Life Technologies) and the correct plasmid verified by Sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... qPCR was carried out using a Step-One plus real time PCR machine (Life Technologies, Paisley, UK) with the following thermal conditions ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using the SuperScript III Platinum One-Step qRT-PCR Kit (Thermo Fisher Scientific) following the manufacturer’s instructions with the following changes ...
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bioRxiv - Microbiology 2020Quote: ... which were collected in Greiner Bio-One VACUETTE Z Serum Sep Clot Activator Tubes (Thermo Fisher Scientific). Samples were run on both machines the day of collection shortly following collection such that they were not stored prior to analysis ...
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bioRxiv - Immunology 2020Quote: ... One microgram of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). TaqMan Fast Universal Master Mix (2X ...
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bioRxiv - Molecular Biology 2021Quote: ... Nucleic acid concentration was measured using NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Fisher Scientific, USA)
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bioRxiv - Immunology 2020Quote: ... Virus detection was performed using One-Step RT-PCR SuperScript(tm) III Platinum(tm) Kit (Life Technologies). Thermal cycling was achieved at 55°C for 10 minutes for reverse transcription ...
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bioRxiv - Developmental Biology 2021Quote: ... One µg of total RNA was reversed transcribed using the Maxima cDNA synthesis kit (Thermo Scientific: K1642). Quantitative PCR was performed using the BioRad LFX96 real-time system ...
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bioRxiv - Genetics 2020Quote: ... One day prior to co-transfection OmB cells were seeded in 96-well plates (Thermo Fisher Scientific) at a density of 2 x 104 cells per well ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Real time PCR analysis was performed using a Step One Plus real-time PCR system (Applied Biosystems, Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was carried out with a Step-One PLUS Fast Real-time PCR cycler (Applied Biosystems) under standard fast cycling conditions using PowerUP SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrations of the peptide stock solutions were measured as described (24) using NanoDrop One (Thermo scientific) at 205 nm and the extinction coefficients listed in Table 1 ...
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bioRxiv - Bioengineering 2022Quote: ... DNA was eluted in 50 μL elution buffer and measured using a NanoDrop one (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... After transformation in bacteria (One Shot™ TOP10 Chemically Competent E. coli; Thermo Fisher Scientific, Bleiswijk, Netherlands), successful cloning was validated by sequencing ...
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bioRxiv - Genomics 2022Quote: ... The integrity of the RNA was determined with NanoDrop One Microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 system (Agilent Technologies) ...
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bioRxiv - Genetics 2022Quote: ... 5uL of ligation reaction was transformed into 25uL of One Shot TOP10 Chemically Competent E.Coli (ThermoFisher C404003) following the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2022Quote: ... One microgram of total RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis System (Invitrogen) with random hexamers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Urate concentration in the medium was monitored using A293 absorption with NanoDrop One (Thermo Fisher Scientific, USA) at the indicated time points 15 ...
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bioRxiv - Pathology 2022Quote: ... one μg of RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (Life Technologies #4368814). Two step qRT-PCR reactions contained 2% of the cDNA product ...
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bioRxiv - Plant Biology 2022Quote: One μg of RNA was used to obtain cDNA using the RevertAid kit (Thermo Scientific, United States) and random hexamers as primers following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA concentration of samples was precisely measured using a Nanodrop™One (Thermo Fisher Scientific, USA).
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bioRxiv - Microbiology 2022Quote: ... with specific primers (Table S4) and detected by Step One Plus Real time PCR machine (Applied Biosystems). Relative quantification of gene expression was performed using the comparative Ct Method (52 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA purity and concentrations were estimated using a NanoDrop One Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... and the total DNA quality and quantity were evaluated using a NanoDrop ONE spectrophotometer (Thermo Scientific, USA) and pooled for subsequent analyses ...
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bioRxiv - Physiology 2023Quote: ... Intact dissected ovaries were incubated for one hour at room temperature in 100 μM EdU (Molecular Probes) in Grace’s insect medium ...
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bioRxiv - Plant Biology 2023Quote: ... One μg of peptides from each sample were analysed by liquid nano-chromatography (Vanquish Neo, Thermo Scientific) coupled to a high-resolution mass spectrometer (Q-Exactive HF (Thermo Scientific) ...
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bioRxiv - Physiology 2022Quote: ... 1% Nonidet P-40] with one tablet (per 10 mL) of protease and phosphatase inhibitor (Life Technologies). After lysis on ice for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the ligated DNA was transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific, Cat# C7373-03) and selected on LB agar plates with 100 µg/mL Ampicillin sodium (Fujifilm ...
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bioRxiv - Molecular Biology 2024Quote: ... Assessment of RNA purity and concentration was examined using Nanodrop (Thermo Fisher Scientific, Cat# ND-ONE-W), while the RNA integrity number (RIN ...
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bioRxiv - Neuroscience 2024Quote: ... the concentration and purity of nucleic acids were assessed using a NanoDrop One spectrophotometer (Thermo Fisher Scientific). RNA integrity was evaluated using an Agilent 6000 NanoKit on a 2100 Bioanalyzer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... One ng of library (composed of library and ERCC spike-in controls, Life Technologies Inc., Carlsbad, CA) was added as input ...
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bioRxiv - Bioengineering 2024Quote: ... RNA concentration was quantified using a Thermo Scientific NanoDrop™ One UV–Vis spectrophotometer (Thermo Fisher Scientific) followed by synthesis of complementary DNA (cDNA ...
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bioRxiv - Bioengineering 2024Quote: ... RNA concentration was quantified using a Thermo Scientific NanoDrop™ One UV–Vis spectrophotometer (Thermo Fisher Scientific). RNA integrity (RIN ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplification was performed using a Step One Plus real time PCR system (Applied Biosystems, Warrington, UK) with TOPrealTM qPCR 2X PreMIX (Enzynomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... YmoB-YmoA-pEXP5-CT was chemically transformed to One Shot™ BL21 Star™ (DE3) (ThermoFisher Scientific) for expression ...
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bioRxiv - Pathology 2023Quote: ... The purity as well as equal loading (40γ) of the protein was determined by Nanodrop One (Thermofisher). To normalize target protein expression ...
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bioRxiv - Genomics 2023Quote: ... DNA concentrations of all samples were assessed using a NanoDrop One spectrophotometer and a Qubit (Thermo Scientific). Initial DNA concentration are reported in Table S1 ...
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bioRxiv - Genomics 2024Quote: ... with one-third of the UTP concentration comprised of Bio-16-UTP (Thermo Fisher Scientific, Waltham, MA). The 20 µL reactions were incubated for 16h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR was performed using a Step-One real-time PCR system (Applied Biosystems, Waltham, MA, USA) with THUNDERBIRD SYBR qPCR Mix (#QPS-201 ...
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bioRxiv - Immunology 2024Quote: ... using the AgPath-ID™ One-Step RT-PCR kit (Life Technologies, Zug, Switzerland, cat. no. AM1005) with vRNA segment 7-specific oligonucleotide primers and probe (50,51) ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for one hour at room temperature (1:1000, Texas Red Goat anti-Mouse IgG, Invitrogen T2767), and similarly washed ...