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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Genes were then recombined into pEarleyGate 104 and 202 plasmids by LR reaction (Invitrogen) as described previously (Earley et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and EGFP-PTC encoding plasmid (0.5 ug) using the Neon™ transfection system (Invitrogen) with the following parameters ...
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bioRxiv - Immunology 2019Quote: All plasmid transfections were performed with a commercial transfection kit (Thermo Fisher Scientific, USA). Cells were seeded on 12 mm coverslips in 24 wells for imaging or in 6-well plates for qRT-PCR or immunoblotting analyses ...
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bioRxiv - Plant Biology 2019Quote: ... along with the PRH1 coding sequence into a pENTRTM/SD/D-TOPOTM plasmid (ThermoFisher). The construct was subsequently recombined with several Gateway destination vectors ...
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bioRxiv - Synthetic Biology 2019Quote: ... All cells were transfected with desired DNA plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... For plasmid transfection Lipofectamine and for the siRNA transfection Lipofectamine RNAimax (Invitrogen, NY, USA) were used according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... cells were transfected with appropriate plasmids using Lipofectamine 3000 according to manufacturer’s protocol (Invitrogen, Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2020Quote: ... Each plasmid was transformed into One Shot® Stbl3™ chemically competent cells (Invitrogen) and purified by ZymoPURE plasmid Maxiprep kit (Zymo research) ...
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bioRxiv - Immunology 2020Quote: ... Lentiviral pseudoparticles were obtained after plasmid transfection of 293FT cells using Lipofectamine 2000 (Invitrogen). The lentivirus-containing media was harvested 48 or 72 h after transfection and concentrated 40 – 50 times using Lenti-X concentrator (Takara Clontech) ...
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bioRxiv - Molecular Biology 2019Quote: Plasmid transfections were carried out in 40 ml cultures of HEK 293T cells (Invitrogen) that had been adapted to grow in suspension ...
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and transiently transfected with the pIRES2-mCherry-YFPCFTR plasmid using Lipofectamine 2000 (Life Technologies). After transfection ...
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bioRxiv - Biochemistry 2019Quote: ... and eGFP and mCherry plasmids were transformed into One Shot Top 10 cells (Invitrogen). A single colony was used to inoculate a 10 mL 2x YT overnight culture supplemented with 200 μg/mL of Carbenicillin (Carb) ...
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bioRxiv - Genomics 2020Quote: ... BamHI-linearized plasmids were in vitro transcribed with T7 RNA polymerase (Thermo Fisher EP0111). Synthesized RNA transcripts were verified by agarose gel electrophoresis and purified using NucleoSpin RNA II column (Machere-Nagel ...
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bioRxiv - Cancer Biology 2020Quote: ... were transfected along with the vector plasmid using Lipofectamine 3000 (Thermo Fisher, Cat# L3000) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2020Quote: ... All cells were transfected with desired DNA plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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HIV-1 Nef interacts with LMP7 to attenuate immunoproteasome formation and MHC-I antigen presentationbioRxiv - Microbiology 2019Quote: ... All cell lines were transfected with plasmids using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... plasmids and transferred to pMDC32 using the Gateway™ LR Clonase™ II (Invitrogen) technique ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.2 µg of pFlag-CMV-Ago2 plasmid were transfected using Lipofectamine 2000 (Invitrogen, #11668019). Cells were harvested three days after transfection.
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with appropriate plasmid DNA using Lipofectamine 2000 (Invitrogen Corp., Carlsbad, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids encoding GST-YidC1CT or GST-YidC2CT were transformed into BL21 Star™ (ThermoFisher). GST-YidC1CT expression was induced with 0.5 mM isopropyl-ß-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... 4 μg plasmid DNA was mixed with 250 μl Opti-MEM medium (Thermo Scientific) and 4 μg PEI ...
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bioRxiv - Molecular Biology 2020Quote: ... pMD2-VSVG (envelope vector) and pCDH plasmids co-transfection using Lipofectamine 2000 (#11668019, Invitrogen). Medium containing virus was harvested 48 h after transfection and filtered by 0.45 μm millipore filter ...
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bioRxiv - Synthetic Biology 2020Quote: ... were transfected with 2 µg plasmid DNA and 4 µL Turbofect (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were transfected with NUAK1 plasmid construct using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) and 48 h post-transfection selected in 4 µg/ml puromycin (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... This plasmid was transiently transfected into Expi293F cells using Expi293F expression medium (Life Technologies) at 37°C 8% CO2 rotating at 150 rpm ...
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bioRxiv - Microbiology 2021Quote: ... pENT23-NLuc was generated by Gateway BP reaction between pDONR P2R-P3 plasmid (Invitrogen) and a DNA fragment encoding NanoLuc Luciferase amplified from pNL1.1 (NanoLuc ...
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bioRxiv - Biochemistry 2019Quote: Gene 5 was cloned between the BamHI and HindIII sites of plasmid pSE380 (Invitrogen) to form recombinant plasmid pMS11 (26) ...
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bioRxiv - Immunology 2019Quote: ... we used 5 μg plasmid DNA and Lipofectamine 3000 (Life Technologies, Grand Island, NY) to transfect 5×106 cells in 10 mL DMEM supplemented with 10% FBS ...
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bioRxiv - Genomics 2019Quote: ... and cloned between the BamHI and EcoRI sites into the pCRII-TOPO plasmid (Invitrogen) to give the pCRII-kanMX6 plasmid ...
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bioRxiv - Genetics 2019Quote: ... A donor plasmid was cloned using 3-fragment MultiSite gateway assembly (Invitrogen, 12537-023) with a 5’ entry element of ~500 bp of the genomic sequence upstream of the ApoBb.1 stop codon ...
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bioRxiv - Genetics 2020Quote: ... we transfected SlugCas9 (300 ng) + gRNA plasmids (200ng) by Lipofectamine 3000 (1ul) (Life Technologies). Cells were collected 5-7 days after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... 2μg of DNA plasmid was mixed with 5μl of Lipofectamine 2000 (ThermoFisher Scientifics, #11668019) in serum-free medium to a total volume of 200μl and incubated at room temperature for 5min before adding to 3×105 cells in a 6-well plate.
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bioRxiv - Physiology 2020Quote: ... The purified PCR products were cloned into plasmid vectors (pCR 2.1 TOPO TA, Invitrogen, and pJet 1.2/blunt ...
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bioRxiv - Cell Biology 2020Quote: All plasmid transfections to Huh7 and HEK293 cells were done with Lipofectamine 2000 (Invitrogen), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Transfections of plasmid DNA were performed using Lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid was then transfected into HepG2 cells with Lipofectamine 3000 (Invitrogen, Carlsbad, CA), followed by selecting positive cells with puromycin (2.5 µg/ml) ...
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bioRxiv - Biochemistry 2021Quote: Plasmid DNA carrying the EGFR sequence was transformed into DH10Bac competent cells (ThermoFisher Scientific) to produce the bacmid DNA ...
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bioRxiv - Molecular Biology 2021Quote: ... All DSB-Spectrum plasmid propagation was done in recombination-deficient bacteria (Stbl3; ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... with heavy chain and light chain plasmids using an ExpiCHO™ Expression System (Gibco). Cell culture was harvested after an 8-to 14-day incubation at 37C with humidified atmosphere of 8% CO2 with shaking ...
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bioRxiv - Cell Biology 2020Quote: ... A plasmid encoding NGPKA-RIα was generated using the Gateway cloning system (Life technologies) by LR clonase reaction of pEF5/FRT/NG-DEST with pENTR-PRKAR1A (obtained from Addgene ...
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bioRxiv - Immunology 2020Quote: ... Lentiviral pseudoparticles were obtained after plasmid transfection of 293FT cells using Lipofectamine 2000 (Invitrogen) or TurboFectin 8.0 Transfection Reagent (Origene) ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were transfected into A18945 iPSC line using Lipofectamine-Stem transfection reagent (ThermoFisher #STEM00001) and GFP-positive cells were collected by fluorescence-activated cell sorting (FACS ...
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bioRxiv - Neuroscience 2021Quote: ... and transiently transfected with a combination of DNA plasmids by using lipofectamine 3000 (Invitrogen). To examine CB knockdown effects ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection of BDNF or YY1 plasmid was achieved with Lipofectamine 3000 (Thermo Fisher Scientifics) according to Manufacture’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... Then plasmids (2 μg/well) were transfected into cells using Lipofectamine® 3000 (Invitrogen). After 48 h cells were lysed in RIPA (Biosharp ...
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bioRxiv - Cancer Biology 2020Quote: ... plasmids were transfected using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, PA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... indicated plasmids and SiRNAs were transfected using Lipofectamine 3000 (L3000015, Invitrogen Carlsbad, CA, USA).
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with each plasmid for 5 h using Lipofectamine 2000 (Life Technologies) and further incubated for 24-48 h ...
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bioRxiv - Cell Biology 2019Quote: ... and cloned between the SalI and Notl sites of the pEntr 1A plasmid (Invitrogen).