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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: To transfect the plasmids overexpressing the transcription factors we used Lipofectamine 3000 (Invitrogen L3000001) reagent ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Immunology 2023Quote: ... 1 µg of the SAMD9L plasmid was transfected with Lipofectamine™2000 (ThermoFisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... siRNAs and plasmids were transfected into cells with RNA IMAX or Lipofectamine 2000 (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... reagents plasmids and Lipofectamine 3000 were mixed in opti-MEM (Thermo Scientific, MA, USA) supplemented with 4mM glutamine and co-incubated for overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The CRISPR plasmid was cotransfected into cells using Lipofectamine 3000 transfection reagent (L3000015, Invitrogen). Forty-eight hours following transfection ...
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bioRxiv - Cell Biology 2023Quote: Plasmid DNA was transfected into all cell types using Lipofectamine 2000 (Invitrogen Cat# 11668019) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Transfection with heavy and light chain plasmids was performed using the Expifectamine293 kit (Gibco) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Each plasmid was transformed into BL21 (DE3)pLysS chemically competent Escherichia Coli cells (Invitrogen, La Jolla ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and 4 ug of the plasmid being tested was transfected using Lipofectamine 3000 (Invitrogen) for 24 hours according to the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2023Quote: Single plasmids were chemically transformed into One Shot STBL3 chemically competent coli (Invitrogen C737303). Bacterial cultures were shaken at 225 rpm for one hour at 37°C and then plated on an ampicillin agar dish ...
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bioRxiv - Molecular Biology 2023Quote: Pooled plasmid libraries were electroporated into ElectroMAX™ Stbl4™ electrocompetent cells (Invitrogen 11635018) and spread on to bioassay plates ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 ng of CMV-Rluc reporter plasmids (78) using Lipofectamine 3000 (Invitrogen L3000015).
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bioRxiv - Plant Biology 2024Quote: ... genes were inserted into the ChampionTM pET300-NT-DEST plasmid (ThermoFisher Scientific, Waltham, MA), before being transformed into Rosetta(DE3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 600 ng of each sgRNA plasmid were mixed together with 0.5 μL BpiI (ThermoFisher), 1 μL of 10X Buffer G (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... and EGFP-LAMP1 genes into the doxycycline-inducible lentiviral expression plasmid pTRIPZ (Thermo Fisher). Lentiviruses were produced by transfecting HEK293T cells with plasmids encoding VSV-G ...
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bioRxiv - Molecular Biology 2023Quote: ... 2-4 μg of plasmid was mixed with 500 μL Opti-Mem (Gibco 31985070), after which 2.5 μL Plus Reagent and 8 μL Lipofectamine LTX (Invitrogen 15338100 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with plasmids (described previously [21]) using the NeonTM Transfection System (Invitrogen). A total of 5×106 cells in 10-cm tissue culture plates were transfected with 25 µg of PX458 plasmid containing cloned sgRNA using the electroporation method (1100 V ...
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bioRxiv - Genomics 2023Quote: Plasmids were transfected into HEK293XT cells using Lipofectamine 3000 (Thermo Fisher Scientific, Cat # L3000015). To prepare HEK cells for transfection ...
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bioRxiv - Bioengineering 2023Quote: ... which was achieved by using GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific, Cat.#. K0502). Plasmid isolation protocol was applied according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2023Quote: ... gene fragments were amplified from plasmids by PCR using Platinum Pfx DNA polymerase (ThermoFisher). Constructs were assembled by Gibson assembly using the Gibson Assembly® Master Mix (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... The plasmids were then transformed in One Shot TOP10 chemically competent cells (Thermofisher, #C404010), amplified ...
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bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: ... Each resultant plasmid was co-transfected with ViraPower™ Lentiviral Packaging Mix (ThermoFisher # K496000) into 293FT cells to make virus particles ...
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bioRxiv - Developmental Biology 2023Quote: ... to enrich cultures for mini-prep (Gene Jet Plasmid Miniprep kit k0503, Thermo Fisher). Purified plasmids were sent for sequencing (Eurofins ...
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bioRxiv - Immunology 2023Quote: ... and the fused cDNA was cloned into the pcDNA3.1 expression plasmid (Invitrogen, Calsbad, CA). For visiualization of EROS ...
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bioRxiv - Plant Biology 2023Quote: ... CDS were then integrated into the pDONR207 plasmid using BP clonase II mix (Thermofisher). pDONR207 plasmids containing PpSMXL CDS were submitted to PCR-mediated mutagenesis to obtain ΔRGK/RT versions.
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bioRxiv - Plant Biology 2024Quote: ... Their PCR products were cloned into pJET1.2 blunt plasmids (Thermo Fisher Scientific, Massachusetts, USA) and sequenced.
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bioRxiv - Developmental Biology 2023Quote: ... Linearized plasmids were subjected to invitro transcription using a SP6 Transcription Kit (Invitrogen AM1340). The resulting mRNA was purified (Total RNA Kit I ...
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bioRxiv - Cell Biology 2024Quote: ... All plasmid DNA was quantified by UV-Vis absorbance using a NanoDrop spectrophotometer (ThermoFisher). All sequences were verified by sequencing performed by Eurofins Genomics (Eurofins Genomics LLC ...
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bioRxiv - Genetics 2024Quote: ... 10.8ug of plasmid was transfected onto 106 cells using the 100uL Neon system (ThermoFisher) with a single ...
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bioRxiv - Developmental Biology 2024Quote: ... and transient transfection with plasmid DNAs was performed using Lipofectamin 3000 (Thermo Fisher Scientific), when cells reached between 60-70% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... GAG-POL and pInducer lentiviral plasmids carrying the transgene using Lipofectamine 3000 (Thermo Fisher). After 24h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with plasmids using the Neon Transfection System (Life Technologies, Carlsbad, CA), according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid transfections were performed using the LipofectamineTM 2000 Transfection Reagent (Thermo Fisher Scientific, 11668500).
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bioRxiv - Genetics 2024Quote: ... coli under appropriate conditions and purified using GeneJET plasmid miniprep kit (Thermo Fisher Scientific) using included instructions ...
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bioRxiv - Immunology 2024Quote: ... and pLP2 plasmids into HEK293T cells at 80–90% confluency using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... FaDu cells were transfected with 500 ng of plasmid according to manufacturer’s instructions (ThermoFisher) and cells were single cell sorted 48 hours after transfection.
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were transiently transfected with lipofectamine 2000 (Life Technologies, catalog no. 11668-027) into cancer cells as stated in the figures.
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bioRxiv - Biophysics 2024Quote: ... Plasmid constructs were transfected into the cells by using Lipofectamine 2000 reagent (Invitrogen, 11668019). After 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Flat-Bottom Microplate with pcDNA vector plasmids using PLUS and Lipofectamine Reagents (ThermoFisher Scientific). Plasmids contained genes for human or zebrafish TLR4 complex components and Renilla luciferase enzyme under constitutively active promoters ...
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bioRxiv - Neuroscience 2024Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... was mixed with each μg of plasmid in Opti-MEM medium (Thermo Fisher Scientific) and added to the cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... combining a total of ∼1.0 μg of plasmid DNAs with serum-free OptiMEM (Gibco) to a final volume of 100 μL ...
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bioRxiv - Cell Biology 2024Quote: ... plasmid transfections were performed using Lipofectamine™ 3000 transfection reagent (Thermo Fisher, #3000-008) or ViaFect (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The guide RNA-Cas9 plasmid was transfected into A549 cells using Lipofectamine 3000 (ThermoFisher) and a cell line-specific protocol provided by ThermoFisher (58) ...
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bioRxiv - Immunology 2024Quote: ... and specific sgRNA-cloned plasmids using Lipofectamine 3000 (Thermo-Fisher) and OptiMEM medium (Gibco) according to the manufacturer’s instructions for 72 hours ...
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bioRxiv - Microbiology 2024Quote: ... HeLa cells were transfected with pcDNA3.1-GFP plasmids using Lipofectamine LTX (Thermo Fisher Scientific). Cells were fixed with 4% formaldehyde 18h post-transfection and the nucleus was stained using DAPI (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... ASO and plasmids at 60–80% confluency was performed using Lipofectamine 2000 (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The plasmids were transfected into HEK293T cells using Lipofectamine™ 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...