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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Mouse NXPE4 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... phospho-tau (Ser202, Thr205) mouse monoclonal antibody (AT8, catalog #MN1020), and tau mouse monoclonal antibody (HT7, catalog #MN1000) from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blotting was performed with 1:5000 dilution of mouse monoclonal anti-DBP5 and 1:10000 dilution of mouse monoclonal anti-GAPDH (Thermo Fisher) primary antibody overnight at 4°C and 1:10000 anti-mouse DyeLight 650 secondary to detect protein.
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bioRxiv - Biophysics 2024Quote: ... blocked with 1% BSA and stained with primary Anti-mouse laminin α5 (gift from Lydia Sorokin) and secondary Anti-mouse IgG AF594 (Invitrogen, A11005). Between all steps ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Molecular Biology 2023Quote: ... blocked with 1 %BSA/ 1%DMSO/PBS for 1 hr at room temperature and immunostained with mouse anti-SV2 AB2315387 and secondary Alexa Fluor 555 anti-mouse antibody from Molecular probes, Eugene.
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bioRxiv - Physiology 2023Quote: ... or SC71-S (1:25, Mouse IgG1, DSHB) followed by Alexa Fluor 488 or 647 (1:500, Goat anti-mouse, ThermoFisher Scientific). Images were acquired using a confocal microscope (Zeiss Axiovert 200 ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 mouse embryos and grown in ‘MEF medium’ containing DMEM (41966029, Thermo Fisher Scientific) supplemented with 10% FBS (10270106 ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos were incubated for 2h at RT with Alexa Fluor 488 nm anti-mouse or Alexa Fluor 568 nm anti-mouse and Alexa Fluor 647 nm anti-goat secondary antibodies (Life Technologies) along with DAPI counterstaining (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... goat anti-mouse Alexa Fluor Plus 488 and F(ab’)2-goat anti-mouse Alexa Fluor Plus 555 from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) and HRP conjugated Goat anti-mouse IgM (#31440) for immunoblotting were from Thermo Fisher. APC conjugated anti-human ganglioside GD2 antibody (#357306 ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA, Invitrogen, respectively). Serum noradrenaline was extracted with the use of a cis-diol–specific affinity gel ...
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bioRxiv - Cancer Biology 2021Quote: ... envelope plasmid pIP/VSV-G (5μg) (ViraPower™ Lentiviral Packaging Mix, Thermo Fisher Scientific) and 5μg lentiviral expression constructs shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cancer Biology 2021Quote: RNA was transcribed from linearized pGEM plasmids using the MEGAscript T7 Transcription kit (Ambion) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding S variants were transiently transfected into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Cell Biology 2020Quote: ... prior to being recombined into expression plasmids by LR cloning (Life Technologies, 11791-020). Plasmid constructs were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2020Quote: Transfection of plasmid DNA was performed using Lipofectamine LTX reagent or Lipofectamine 2000 (Invitrogen), and all RNA transfections were performed at a final concentration of 50 nM using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were introduced into cells using Lipofectamine LTX Plus Reagent (Invitrogen Cat#15338-100), according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... HEK cells were transiently transfected with plasmid DNA constructs using Lipofectamine 2000 (Invitrogen/ThermoFisher) in optiMEM media (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... HEK cells were transiently transfected with plasmid DNA constructs using Lipofectamine 2000 (Invitrogen/ThermoFisher) in optiMEM media (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... was used to construct a donor plasmid containing the pCR8/GW/TOPO (Thermo Fisher) plasmid backbone ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin-free miniprep or midiprep plasmid DNA transfections were performed using Lipofectamine 2000 (Invitrogen). On the day of transfection ...
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bioRxiv - Immunology 2021Quote: ... by transfection with purified plasmid using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were collected 3 days after transfection ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Genetics 2021Quote: ... Minicircle DNA is then isolated using the Purelink HiPure Plasmid Filter Midiprep kit (Invitrogen). To ensure complete removal of the parental plasmid ...
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bioRxiv - Genetics 2021Quote: ... Cells at 80% confluency were transfected with plasmids using Lipofectamine 2000 (Life Technologies Ltd.) as described previously26.
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bioRxiv - Microbiology 2020Quote: ... Plasmid was ligated using 1 μL (1U/μL) T4 DNA Ligase (Invitrogen, 15224-017) for 5 min at room temperature and subsequently 2μl plasmid was transformed into 100μl chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... they were transfected with the reporter plasmid in the presence of JetPei reagent (Invitrogen). They were incubated for 14 h and then rinsed with fresh medium ...
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bioRxiv - Molecular Biology 2021Quote: ... wild-type or mutant IPMK cDNAs were subcloned into pFASTBAC1 plasmid (Thermo Fisher Scientific) with an N-terminal FLAG epitope ...
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bioRxiv - Developmental Biology 2021Quote: ... E14Tg2a ESCs were transfected with the eSpCas9 plasmids using lipofectamine 3000 (Invitrogen cat. L3000001). After 24 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection was performed using Lipofectamine™ 2000 (Thermo Fisher Scientific, Waltham, MA, USA) or Viafect (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were transfected with two sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Lot # 2125386), and 24 hours later ...
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bioRxiv - Evolutionary Biology 2021Quote: ... linearized recombinant pCS2+ plasmids using the mMESSAGE mMACHINE SP6 transcription kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2020Quote: Plasmids were sequenced using either BigDye™ Terminator v3.1 sequencing kit (Applied Biosystems, 4337458) and analyzed in house or samples were sequenced commercially using the GENEWIZ sanger sequencing service.
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bioRxiv - Immunology 2022Quote: ... , we constructed the expression plasmids by synthesizing the DNA fragments from GeneArt (Life Technologies) and cloned them into the phCMV3 vector (Genlantis ...
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bioRxiv - Neuroscience 2022Quote: ... cells were transfected with 2.5 µg GFP-GLT-1 plasmid using Lipofectamine 3000 (Invitrogen) (1:2 DNA/Lipofectamine ratio) ...
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bioRxiv - Molecular Biology 2021Quote: ... Transfection of plasmids or siRNAs was performed using Lipofectamine 3000 or RNAiMAX (Life technologies) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... All plasmids were transfected into U2OS or HaCaT cells using Lipofectamine 3000 (ThermoFisher Scientific) or Xfect Transfection Reagent (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... The target plasmid used in the assays is a pJET1.2 derivative (Thermo Fisher Scientific). The plasmid was added to the reaction mixtures to the final concentration of 2 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... to generate expression plasmids for the Bac-to-Bac baculovirus expression system (Thermo Fisher). Mutations were made using the wild-type EfPiwi expression plasmid as a template and amplifying the protein using oligonucleotide primers containing the desired mutation ...
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bioRxiv - Molecular Biology 2021Quote: ... we mixed 500 ng of plasmid DNA in 50 μl of OptiMEM (ThermoFisher Scientific) and 3 μl of Lipofectamine 2000 in 47 μl of OptiMEM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... STBLII cells were used for maintenance of plasmids containing tandem repeats (Invitrogen cat#10268019). Primer sequences for plasmid constructions are given in Table 1 ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC DNA extraction was performed using Hi-Pure Plasmid DNA Extraction Kit (Invitrogen K210017). Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910) ...
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bioRxiv - Neuroscience 2019Quote: ... The Cre-NLS (from the pBS185 plasmid from the former Life Technologies, Gaithersburg, MD) and SV40 poly A signal (from the pTRE-tight plasmid from Clontech Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... but plasmid DNA was delivered in serum-free OptiMEM (Gibco, Fisher Scientific, Waltham MA). A ratio of 3:1 (3 µL of HP reagent ...
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bioRxiv - Cell Biology 2019Quote: ... but plasmid DNA was delivered in serum-free OptiMEM (Gibco, Fisher Scientific, Waltham MA). A ratio of 3:1 (3 µL of HP reagent ...