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Citations for Thermo Fisher :
4201 - 4250 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... ELISAs were performed using FBLN5 (Fibulin-5) Human ELISA Kit from Fine Test (EH0772) and Thrombospondin 1 (TSP1) Human ELISA Kit from Invitrogen (BMS2100), following the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: Silencer Select siRNA negative control (4390844) and those targeting GFAT1 (human, s5708; mouse, s66610) or GFAT2 (human, s19305) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... for each field.15 Analysis of cytokine secretion in primary human AML samples was performed using the Cytokine/Chemokine/Growth Factor 45-Plex Human ProcartaPlex™ system (ThermoFisher).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Immunology 2022Quote: ... and activated for 48h with 30 U/ml human IL-2 and anti-CD3-anti-CD28-beads (Dynabeads™ human T-Activator CD3/CD28, ThermoFisher). Retroviral packaging cells RD114 were seeded to reach a confluency of 60% on the day of transfection and subsequently transfected with plasmids containing the neoTCR-α- and -β-chain-sequences using TransIT®-293 (MirusBio) ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...
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bioRxiv - Immunology 2024Quote: ... blocked then incubated overnight at 4°C in primary antibody (rabbit anti-human STAT1 1:500, HPA000931, Sigma-Aldrich; mouse anti-human pSTAT1 1:1000, 33-3400, Invitrogen; mouse anti-human STAT2 ...
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2023Quote: ... Human serum used for all conditions was from the same lot of pooled human AB serum (Fisher Scientific, Cat#: BP2525-100). For heat inactivation ...
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bioRxiv - Neuroscience 2024Quote: ApoE and sAPP were measured in human CSF samples using the Apolipoprotein E Human ELISA Kit (#EHAPOE) and Amyloid Precursor Protein Human ELISA Kit (#KHB0051) kits respectively from Life Technologies, following the manufactureŕs protocol in duplicates using a 1:100 dilution of CSF.
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bioRxiv - Microbiology 2019Quote: Human IL-18 and TNFα were measured with the human IL-18 ELISA Kit (MBL, Woburn, Massachusetts) and human TNFα ELISA Kit (ThermoFisher, Waltham, Massachusetts) according to the manufacturers’ instructions using cell culture supernatant at a 1:5 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Human brain samples classified into healthy (n=47) and AD (n=32) were processed by array (Affymetrix Human Gene 1.0 ST Array)53 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Telomerase-immortalized human neonatal foreskin keratinocytes (N-TERTs) (21) were cultured in EpiLife medium containing human keratinocyte growth supplement (HKGS) (Thermo Fisher Scientific) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... 1% MEM NEAA and 50 Units/mL recombinant human IL-2 (Myltenyi; #130-097-745) with Dynabeads™ Human T-activator CD3/CD28 (Gibco, #11132D) the same day of the purification (day 0 ...
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bioRxiv - Microbiology 2020Quote: ... 0.01ug/ml soluble human anti-CD3 (R&D clone UCHT1) and 0.1 ug/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) at a concentration of 1×106 cells/ml in RPMI-1640 (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... SH-SY5Y human neuroblastoma (ECACC) and RH-30 human rhabdomyosarcoma (kindly donated by Marc-David Ruepp) were maintained in DMEM (Thermo Fisher Scientific), supplemented with 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Immunology 2021Quote: ... and the biotinylated secondary anti-human IgG (ref. B1140-1ML, MilliporeSigma, St. Louis, USA)] and the anti-human IgE (ref. A18803, Invitrogen, Waltham, USA) antibodies were added at 1:1250 and 1:100 dilutions ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™, Life Technologies Corporation ...
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bioRxiv - Immunology 2024Quote: ... The cells were then washed in PBS and stained with a mix of secondary antibodies anti-human IgG-Fc-AF647 (1:600) and anti-human IgA-Alpha-Chain-AF488 (1:200) (Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 0.01 µg/ml soluble human anti-CD3 (R & D; clone UCHT1) and 0.1 µg/ml soluble human anti-CD28 (Life Technologies; clone CD28.2) for 3 days before infection ...
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bioRxiv - Molecular Biology 2023Quote: ... All hFRG mice reported in this study were engrafted with human and non-human primate hepatocytes from the same donors (Caucasian, 15-month-old donor, Lonza, #HUM181791; ThermoFisher, #CY409, respectively).
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bioRxiv - Biochemistry 2024Quote: ... The preparations of insect cell microsomes containing human CYP2A6 (lot 2520331) and CYP2D6 (lot 2350106) along with human CPR and cytochrome b5 (Baculosomes®) were procured from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genomics 2024Quote: Dataset 3: This dataset includes the Human Origins SNP Array genotypes (2,583 present-day individuals genotyped on the Affymetrix Human Origins Array) merged with ancient individuals from SW Asia and S Europe called across a total of 616,938 autosomal SNPs (Lazaridis et al. ...
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bioRxiv - Biophysics 2021Quote: MRC5 cells (human lung fibroblasts, ATCC® Cat# CCL-171™, RRID:CVCL 0440) were maintained in MEM media (Cat# 11095080, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2019Quote: ... Jurkat and Human B-lymphoid cell lines were initially tested for mycoplasma contamination and then cultured in RPMI-1640 medium (Life Technologies, Zug, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2019Quote: The cytotoxicity of fluoro-TEMPO and CTEMPO against human T24 urinary bladder epithelial cells was examined utilizing the Pierce™ LDH cytotoxicity assay kit (Life Technologies, Australia) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... was performed on the Rituximab expressing cell pool by staining with an anti-human IgG antibody conjugated to FITC (Invitrogen, Carlsbad, CA, USA) and subsequent single-cell sorting using fluorescence activated cell sorting (FACS ...
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bioRxiv - Genetics 2021Quote: The human hepatocellar carcinoma (HCC) cell line SNU-398 was cultured in Roswell Park Memorial Institute 1640 medium (RPMI) (Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2020Quote: CD4+ T cells were electroporated prior to stimulation with anti-CD3/CD28 beads (Dynabeads Human T-Activator CD3/CD28, Thermo Fisher Scientific (ThermoFisher), Waltham ...
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bioRxiv - Molecular Biology 2022Quote: ... H1944 (human lung adenocarcinoma, ATCC, CRL-5907), and H441 (human lung adenocarcinoma, ATCC, HTB-174) cells were maintained in RPMI 1640 medium (Thermo Fisher Scientific, A1049101) with 10% FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: Human embryonic kidney cells (HEK293T, ATCC, Cat #CRL-3216) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher Scientific, Invitrogen, Cat#11965092) supplemented with 10 % (v/v ...
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bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific, Life Technologies, Item #11330-032) supplemented with 10% Fetal Bovine Serum (Atlanta Biological ...
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bioRxiv - Bioengineering 2021Quote: ... MDA-MB-231 (ATCC) and MCF7 (ATCC) human mammary cancer cells were both cultured in 1:1 DMEM/F-12 (Thermo Fisher Scientific, USA) media supplemented with 10% fetal bovine serum (Atlanta Biological ...
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bioRxiv - Immunology 2021Quote: ... The pcDNA 3.1+ vectors were then transfected individually into human embryonic kidney (HEK293T) suspension cells using ExpiFectamine 293 transfection reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: The whole genome siRNA screen was conducted as described (35) using HeLa S3 cells and the Dharmacon ON-TARGETplus® SMARTpool® human siRNA library (Thermo Scientific) and strain T3SA+.
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bioRxiv - Cell Biology 2021Quote: Human epithelial colon adenocarcinoma, Caco2, cells (ACC 169, DSMZ, Leipzig, Germany) were grown in DMEM/F-12 (Gibco, Thermo Scientific, Waltham, Massachusetts, USA) supplemented with 10 % heat inactivated fetal bovine serum (Biowest ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol exportbioRxiv - Cell Biology 2021Quote: ... cells were transfected with siRNAs targeting human SNX13 (5’- CAGAAAGGCUCAACAGAAAUU-3’) or SNX14 (5’-GGAUGAAAGUAUUGACAAAUU-3’) using Lipofectamine RNAiMax (Invitrogen, Cat#13778-075) according to the manufacturer ...
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bioRxiv - Neuroscience 2021Quote: Post-selection iPSCs with stably-integrated human NGN2 under a tetracycline-inducible promoter were dissociated into single cells using Accutase (Thermo Scientific Cat. #A1110501) and plated on a Matrigel (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were stained for 30 min on ice using AlexaFluor700-conjugated anti-Human CD54/ICAM-1 (clone 1H4, Life Technologies, #MA528553, 1/75), PE-Cy7-conjugated anti-Human CD106/VCAM-1 (clone STA ...
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bioRxiv - Cell Biology 2022Quote: ... MCF10A human breast epithelial cells (CRL-10317, ATCC, Manassas, VI, USA) were maintained in DMEM/F12 medium (11039-021, Invitrogen, Carlsbad, CA, USA) supplemented with 20 ng.ml-1 epidermal growth factor (AF-100-122 ...
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bioRxiv - Cell Biology 2020Quote: ... human milk cells were isolated by diluting milk samples in an equal volume of sterile phosphate-buffered saline (PBS) (ThermoFisher Scientific, Waltham, U.S.) and centrifuged at 870 g for 20 minutes at 20°C in a Rotanta 460R centrifuge (Hettich ...
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bioRxiv - Immunology 2020Quote: ... 4 were used to measure the message levels of these human or mouse genes in cells or lung tissue (Life Technologies, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: ... The human embryonic kidney 293T/17 cells (ATCC, CRL-11268) were maintained in maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants were analyzed for secreted proteins using Immune Monitoring 65-Plex Human ProcartaPlex™ Panel for MAGPIX (Thermofisher Scientific, Carlsbad, CA) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... HTB-14) human glioblastoma cells were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F-12 (DMEM/F-12, ThermoFisher Scientific cat. no. 11330057) supplemented with 10% fetal bovine serum (FBS premium grade ...
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bioRxiv - Immunology 2022Quote: ... Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific, Cat.11131D) at 1:1 ratio for two days ...
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bioRxiv - Cell Biology 2024Quote: ... Human MPB CD34+ cells from a healthy volunteer were thawed using chilled Iscove’s Modified Dulbecco’s Medium (IMDM) (Thermo Fisher Scientific, Waltham, MA, USA,) supplemented with 2% fetal bovine serum (FBS) ...