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Citations for Thermo Fisher :
4301 - 4350 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... His-tag recombinant SARS-CoV-2 Spike Protein S1/S2 (S-ECD) (1 ng/ml; ThermoFisher Scientific; aa11-1208; RP-87680) or Flag-tag recombinant SARS-CoV-2 Spike RBD (1 ng/ml ...
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bioRxiv - Genetics 2020Quote: ... the gypsy backbone used in previous publication (11) was amplified and cloned into the NotI/XbaI sites of pAc5.1/V5-His version B (V411020, Thermo Fisher Scientific). To synthesize the final pAc-gypsy-H2B-mCherry vector ...
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bioRxiv - Microbiology 2020Quote: ... and 293T were cultured in high-glucose DMEM (HyClone) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, ThermoFisher Scientific), 100 U/ml penicillin-G ...
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bioRxiv - Genetics 2020Quote: ... the human BBS2 ORF was PCR amplified with gene specific primers and sub-cloned into the pCDNA3.1V5/His TOPO TA vector (Invitrogen, Carlsbad, CA). The GeneArt Site-Directed Mutagenesis system (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Cancer Biology 2022Quote: ... p41Plk3 mammalian expression vector for protein expression and purification was generated by performing an LR recombination reaction between the pENTR/D-TOPO entry clone and a Gateway destination vector pcDNA-DEST40 containing His-tag (Life Technologies). ShPlk3s (shRNA-A ...
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bioRxiv - Developmental Biology 2022Quote: ... Biotin pulldown was performed by adding an equal volume of Hi-C sample with Dynabeads MyOne Streptavidin T1 beads (Invitrogen, 65602) and incubated by 15 minutes with rotation ...
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bioRxiv - Bioengineering 2022Quote: Hi-Def B8 medium was prepared by adding Hi-Def B8 aliquots (Defined Bioscience #LSS-201, San Diego, CA, USA) to DMEM/F12 (ThermoFisher #11320033) along with 1% antibiotic-antimycotic ...
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bioRxiv - Immunology 2022Quote: The extracellular region of the HLA-DPA1*02:01 and HLA-DPB101:01 chains were separately cloned into pMT\BiP\V5-His A (ThermoFisher scientific). The alpha chain construct harbors the acidic leucine zipper and terminates by a 6His-tag ...
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bioRxiv - Plant Biology 2022Quote: ... Each sgRNA was introduced into the shuffle vectors by overlap PCR with Q5 Hi-Fidelity polymerase followed by digestion of the original vector with DpnI (Thermo Scientific). A destination vector harboring UBQ10 promoter ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS and 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) was added to each well ...
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bioRxiv - Microbiology 2022Quote: ... a second agarose overlay containing DMEM (high glucose, +L-Glutamine) with 2% Hi-FBS 0.5% (w/v) UltraPure agarose (Invitrogen, 16-500) and 200 ng/mL rifampicin was added 16 – 24 h after infection ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and incubated at room temperature for 2 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified lysates were added to previously equilibrated His-Pur™ Ni-NTA spin columns (0.2 mL resin bed, Thermo Scientific #88224) and mixed end-over-end at 4°C for 30 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... was used to assess the number of cycles necessary to amplify libraries to the concentration needed for sequencing by amplifying 1 uL of library with LabTAQ Green Hi Rox master mix (Labtech) and adapter-targeted primers on a StepOnePlus Real-Time PCR system (Thermofisher Applied Biosystems). Indexing PCR involved double indexing (Kircher et al ...
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bioRxiv - Plant Biology 2024Quote: ... Constructs were cloned into a Gateway pDest-CTD-His vector via a pDONR intermediate and BP /LR clonase (all reagents Thermo Fisher) following the manufacturer’s instructions (Hartley ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of His-GLPR at 25 μM was incubated with 20x molar excess of EZ-Link NHS-PEG4-Biotin (Thermo Scientific) following supplier instructions ...
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bioRxiv - Genetics 2023Quote: ... The blots containing yeast Nap1 were treated similarly but incubated with mouse anti-HIS primary antibody (Thermo Fisher Scientific, Waltham, MA) and HRP-conjugated sheep anti-mouse secondary antibody (Kindle biosciences ...
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bioRxiv - Microbiology 2023Quote: ... All collected fractions (3 ml) were analyzed using SDS-PAGE and Western blotting with an anti-His-tag antibody (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... The cDNA fragment encoding the full-length Cckbr1 was PCR-amplified and subcloned into the expression vector pcDNA3.1/V5-His-TOPO (Thermo Fisher Scientific). The primers used here are listed in Extended Data Table 3 ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Microbiology 2023Quote: ... Extended products were purified by alcohol precipitation followed by dissolving in Hi-Di formamide and analysing in an ABI3700 automated DNA Analyzer (Applied Biosystems). Raw sequences are assumable and aligned using NCBI blast and submitted to NCBI databank for accession numbers ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Microbiology 2023Quote: ... or pCDNA SARS-CoV-2 ORF7a-V5/His (WT/H47Y; 0.75 μg) or empty vector using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates were cleared by centrifugation at 20,000 rpm for 45 min and the His-SUMO fusion proteins were enriched on Ni-NTA beads (Thermo Fisher Scientific). The protein was eluted using a buffer containing 25 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were digested with EcoRI and XbaI and inserted into the mammalian expression vector pcDNA-V5-HIS-B (Invitrogen Corp.) that had been cut with the same enzymes ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μl of each diluted and multiplexed PCR product was then added to 10 μl of Hi-Di Formamide and 0.1 μl of GeneScan 500 LIZ Size Standard (Applied Biosystems Inc.) to prepare for genotyping ...
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bioRxiv - Biochemistry 2023Quote: ... coli Methionine Sulfoxide Reductase A and B containing N-terminal His tags downstream of a T7 promoter were synthesized (Invitrogen GeneArt) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2024Quote: Purified Sl-Enc1/His-Sl-Enc2 protein samples were analyzed via SDS-PAGE with a 14% Novex Tris-Glycine Gels (Thermo-Fisher Scientific). The SDS-PAGE gel was stained with Coomassie and the overexpressed bands at the correct molecular weights were excised and stored in water ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were cultured in StemPro hESC SFM (Gibco) supplemented with 20ng/ml bFGF (R&D ...
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bioRxiv - Genomics 2021Quote: ... and HLA-DR/human MHC class II antibodies (Invitrogen Dynabeads® ...
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bioRxiv - Immunology 2022Quote: ... and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher) at a 1:1 bead to cell ratio ...
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bioRxiv - Molecular Biology 2020Quote: ... Commercial pooled RNA (Quantigene Human Reference, QS0639, Life Technologies) and pooled human male DNA (G3041 ...
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bioRxiv - Molecular Biology 2021Quote: ... MEFs and human fibroblasts were cultured in DMEM (Invitrogen) supplemented with 10% FBS (Atlanta Biologicals ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and Dynabeads Human T-Activator CD3/CD28 (Thermo Fisher) at 1 bead per cell ...
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bioRxiv - Immunology 2022Quote: ... human recombinant Annexin V (5 μg/ml) (Invitrogen, #BMS306) or with the same amounts of their respective solvent (DMSO or H2O ...
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bioRxiv - Genomics 2020Quote: ... or Mouse anti-human GAPDH monoclonal (MA5-15738, Invitrogen). The secondary antibody was HRP conjugated Goat anti-Rabbit (NA934 ...
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bioRxiv - Cell Biology 2019Quote: Unlabeled LDL (1 mg) from human plasma (Thermo Scientific) was incubated with 50 nmol cholesterol (Top-Fluor cholesterol ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-human CD133 (Thermo Fisher Scientific, 1:1000) or rabbit anti-human Vimentin (Proteintech Group ...
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bioRxiv - Physiology 2019Quote: ... Antibodies: anti-human endoglin (Ref. H300; Thermo Fisher Scientific) and anti-mouse β-actin (Ref ...
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bioRxiv - Cancer Biology 2019Quote: ... Human recombinant bFGF and EGF were purchased from Invitrogen/Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... and 20 ng/ml human recombinant EGF (Thermo Fisher) at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... Rabbit anti-human-Ki67 (1:20, Thermo Fisher Scientific), Rabbit anti-Chromogranin A (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... 20 μg/mL human recombinant insulin (Thermo Fisher Scientific), 20 μg/mL ascorbic acid (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... a secondary goat anti-human Alexa Fluor-555 (Invitrogen) was used as the target sites were bound with anti-human TCB after administration ...
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bioRxiv - Immunology 2021Quote: ... and 10% heat-inactivated human AB-positive serum (Invitrogen) at 37°C in 5% CO2 ...