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Citations for Thermo Fisher :
4201 - 4250 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... HT1080 cells were transiently transfected with Rac1 or RhoA biosensor and Lifeact-RFP670 plasmids 16–24 h before imaging using Lipofactamine 3000 (Invitrogen). Cells used for live cell imaging were seeded on the collagen I coated PAA gel conjugated on 5 mm round glass coverslip in Leibovitz L15 Medium ...
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bioRxiv - Cell Biology 2021Quote: ... the oocytes were incubated with the FITC-conjugated goat anti-mouse IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: Primary antibodies were diluted in 2% bovine serum albumin (BSA)/PBS and incubated for 48 h followed by incubation with fluorophore-coupled secondary antibodies (Alexa488 donkey anti-mouse Invitrogen Cat #A-21202 ...
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bioRxiv - Cancer Biology 2021Quote: Lung and tumour samples were either processed immediately or held intact for a maximum of 48 h at 4 °C in DMEM/F12 media (Gibco) supplemented with 1 mg/mL of penicillin and streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated at ∼ 2×104 cells/ml on poly-L-lysine coverslips and allowed to attach for 24 h before DNA constructs were introduced into cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with secondary antibody (Alexa Fluor 647 F(ab)’2 fragment goat anti-rabbit IgG (H + L) from Invitrogen by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 10’000 rcf for 30 min and then ultracentrifuged at 120’000 rcf at 4 °C for 2 h (TH 64.1 rotor, Thermo Fisher Scientific). The epididymosomal pellet was then washed in PBS at 4 °C and ultracentrifuged at 120’000 rcf at 4 °C for 2 h ...
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bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: Drosophila melanogaster fly stocks were maintained on a 12:12-h light:dark cycle at 50% relative humidity on Jazz-mix (AS153, ThermoFisher Scientific) food or food made in-house using an equivalent recipe ...
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bioRxiv - Developmental Biology 2022Quote: First-strand cDNA was synthesized from a total RNA sample (2 μg) using SuperScript™ II ribonuclease H-RT (Invitrogen) in 20 µl volume in the presence of gene-specific (GS ...
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bioRxiv - Developmental Biology 2022Quote: ... mesodermal differentiation was induced on day 0 for 24 h by changing the medium to cardiac priming medium (RPMI 1640 (Gibco), 1x B27 supplement without insulin (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... secondary antibodies were applied for 2 h in the dark (1:250 anti rabbit IgG coupled to AlexaFluor-555 [Invitrogen, A31572] and 1:250 anti mouse IgG coupled to AlexaFluor-488 [Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... Nuclear RNA was reverse-transcribed to cDNA with the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: 500k BMDMs were seeded on 50 mm2 varied stiffness ECM coated PA-gels and cultured for 24h in 25 mM glucose DMEM or 20-22 h in 25 mM glucose DMEM followed by medium change to DMEM lacking glutamine (Gibco, 11960044 replaced with with 13C5-glutamine (Cambridge Isotope Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibody: goat-derived anti-Rat IgG (H+L) conjugated to Alexa Fluor 488 (AF488) (dilution 1:250; Thermo Fisher). Samples were incubated at room temperature for 1h in the dark ...
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bioRxiv - Cell Biology 2022Quote: The following secondary antibodies were used for immunofluorescence: AlexaFluor 488 goat anti-rabbit IgG (H+L) (Invitrogen, A11034, Lot: 2256692); AlexaFluor 594 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... diluted in 8% BSA PBST were applied for 2 h at room temperature followed by an incubation of HCS Cell Mask Deep Red Stain (Cat. #H32721, Invitrogen) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... The slides were then seeded with COS7 cells and grown for 24 h in a six-well plate using phenol-free Dulbecco′s Modified Eagle′s medium (Gibco) with 1 g/l D-Glucose (i.e. ...
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bioRxiv - Biochemistry 2022Quote: ... and subsequently probed with alexa fluor 488-conjugated anti-His or anti-mouse IgG (H + L) Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (ThermoFisher), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... cells were treated with RSL3 and Ferrostatin-1 or Turofexorate/Fexaramine for 2.5 h before they were harvested using 0.05% Trypsin-EDTA (Thermo Fisher Scientific). Cells were counted and cell number was adjusted to the sample with the lowest cell count ...
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bioRxiv - Cancer Biology 2022Quote: ... Each acyl-CoA was quantified by the [M-507+H]+ product ion with a 5 ppm window with Tracefinder 4.1 (Thermo Scientific). Analysts were blinded to sample identity during sample preparation and analysis.
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysates were incubated for 2 h at 4°C with IgG-coupled Dynabeads (Dynabeads M-270 Epoxy; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The denaturing gel was run for 2 h at 200 V and RNA bands were visualized with SYBR Safe DNA Gel Stain (Invitrogen) under UV light.
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bioRxiv - Cancer Biology 2022Quote: ... 2.5×105 CAR or NT-T cells were incubated with SSEA-4 and SSEA-3 (#GLY131 and #GLY122, respectively, Elicityl-Oligotech) at 200 μM for 48 h in 24 well plate (Nunc). 5×105 CAR and NT-T cells CFSE-labeled were incubated with or without 1×106 OVCAR4 and Caov-3 cells for 48 h in RPMI-10% FBS-Pen/Strep and L-Glut medium in 10 cm cell culture dishes (Nunc) ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 CAR and NT-T cells CFSE-labeled were incubated with or without 1×106 OVCAR4 and Caov-3 cells for 48 h in RPMI-10% FBS-Pen/Strep and L-Glut medium in 10 cm cell culture dishes (Nunc). CFSE dilution was analyzed using flow cytometry on CD3+-gated cells ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was washed 3x for 5 min with TTBS and incubated 1 h at RT under agitation with anti-GFP-HRP (Thermofisher) diluted 2,500 fold in 1% BSA in TTBS ...
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bioRxiv - Biophysics 2022Quote: ... embryos were placed in halocarbon oil and injected using a microinjection setup with ROCK inhibitor (H-1152, 40 mM, Invitrogen), Embryos were immersed in halocarbon oil for spinning disk imaging.
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bioRxiv - Cell Biology 2022Quote: ... sections were washed and incubated for 1 h with Alexa Fluor conjugated secondary antibodies (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Cell Biology 2022Quote: ... The following day slides were washed thrice with blocking buffer for 15 min and incubated for 1 h at RT in secondary antibodies and Hoechst 33342 (Invitrogen) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... 5’ and 3’ 400 bp homology regions flanking the Pkp230p guide were amplified from Pk A1-H.1 genomic DNA and inserted into a plasmid backbone containing a multiple cloning site (synthesized by Geneart, Thermofisher) via SacII/SpeI and BlpI/NcoI restriction sites ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and 24 h later transfected with 1:1 ratio (DNA plasmid:Lipofectamine) using Lipofectamine 3000 reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Genomics 2022Quote: ... Cells were FACS sorted in 384-well plates and lysis of individual cells was performed for 2 h at 55°C using Proteinase K (Ambion) in 1x Cutsmart (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... 18 mm round glass 1.5 H coverslips (Roth®) in the bottom of 24-well polystyrene plates (Thermo Scientific®). Exceptionally ...
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bioRxiv - Immunology 2022Quote: ... NETs/NLS were imaged at 4 h post stimulation using 20X magnification on a EVOS FL Auto Microscope (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (1:200, ThermoFisher Scientific catalogue #A-11011), Goat Anti-Mouse IgG H&L Alexa Fluor 488 preadsorbed (1:200 ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated for 1 h at RT with the corresponding fluorescent secondary antibody diluted in staining buffer (1:1000: Invitrogen), the cell nuclei were stained with Hoechst (10 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Physiology 2022Quote: ... HEK 293 were cultured in DMEM with 10% FBS and transfected with plasmid for 24 h using Lipofectamine 3000 transfection reagent (ThermoFisher). HEK 293 were cultured in 5 or 25 mM glucose ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl of Alexa Fluor 488 goat anti-mouse IgG (H+L) at 2 µg/ml (Life Technologies, cat#A11001) and 0.5 μl of 10 mg/ml Hoechst 33342 was added to the sample and incubated for 15 minutes at RT in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The copper-catalyzed azide-alkyne cycloaddition reaction was conducted for 18 h on the Versette liquid handling robot (ThermoFisher Scientific) at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Three 5-min washes with 1×PBS were then carried out and 1:1000 diluted Alexa Fluor 647 goat anti-mouse IgG H+L secondary antibody A21235 (Invitrogen) was added to each slide ...
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bioRxiv - Cell Biology 2022Quote: ... MBL) and an additional 2 h at 4°C with 20 μL of a slurry of IgG-conjugated Dynabeads (Invitrogen). After washing the beads five times with IP buffer ...
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bioRxiv - Microbiology 2022Quote: ... antibody and the secondary Alexa Fluor 488 (green) labeled goat anti-rabbit IgG (H + L; F (ab’)2 fragment) (Invitrogen) targeting the anti-E ...
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bioRxiv - Microbiology 2022Quote: ... USA) in combination with Alexa Fluor 594 (red)–labelled goat anti-mouse IgG (H+L; F(ab')2 fragment) (Invitrogen) targeting the RSE cell cytokeratins were used (52) ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was synthesized from approximately 800 ng of RNA in a reaction that included Superscript II RNase H reverse transcriptase (Invitrogen) as described by the manufacturer ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was further used to synthesize first-strand cDNA using RevertAid H Minus First Strand cDNA synthesis Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 1 min with F(ab’)2-Goat anti-Rabbit IgG (H+L) Secondary Antibody QDot emitting at 655 nm (1 nM; Invitrogen) in PBS (1 M ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were washed with PBS and incubated for 2 h with secondary antibodies conjugated to AlexaFluor 488 (1:400; Invitrogen). Sections were then rinsed with PBS ...
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bioRxiv - Plant Biology 2022Quote: ... PhnsLTP3-RNAi and PhnsLTP1+ PhnsLTP3-RNAi flowers collected at 15:00 h on day 2 postanthesis were stained in propidium iodide solution (10 μg mL-1, Invitrogen) for 1 h at room temperature with shaking ...