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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS) (Helena Bioscience, Newcastle, UK) and 2 mM glutamine (Gibco Labs) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). After the first passage the antibiotic concentration was reduced to 1%.
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bioRxiv - Cancer Biology 2021Quote: ... Bone marrow cells were isolated by crushing the femur and tibia in PBS containing 2% FCS (GIBCO), and passed through a 70 μm filter.
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bioRxiv - Biochemistry 2021Quote: HEK293T cells were cultured in growth medium (DMEM, Glutamax, 4.5 g Glucose, 10% FCS, 1% PS; Invitrogen) at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 100 U/ml penicillin/streptomycin (P/S, Life Technologies) For knock-down experiments ...
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bioRxiv - Bioengineering 2021Quote: ... A capture antibody solution was prepared by adding 0.02 μg of biotin anti-FC (Thermo Fisher, A18821) per 1 mL of washing buffer ...
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bioRxiv - Microbiology 2021Quote: ... 10% fetal calf serum (FCS, Capricorn Scientific #FBS-11A) and non-essential amino acids (NEAA; Gibco #11140035) one day prior to infection ...
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bioRxiv - Cancer Biology 2021Quote: ... DIFF cells were cultured in 10% foetal calf serum (FCS) containing high-glucose DMEM (Thermo Fisher Scientific) complemented with 2mM glutamine ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS, Labtech) and 100 U/ml Penicillin-Streptomycin (Thermo Fisher Scientific). HEK 293T and HeLa TZM-bl cell lines were grown in DMEM medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... C2C12 myoblasts were propagated in DMEM supplemented with 20% FCS 10 μg/mL gentamicin (Thermo Fisher Scientific). Cells were seeded at 4×103 per well in 96-well tissue culture plates ...
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bioRxiv - Microbiology 2020Quote: ... E6 Vero and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated in Hanks’ Balanced Salt Solution (HBSS) supplemented with 10% dialyzed FCS (all from Invitrogen). SMPD2 inhibitor GW4869 was used at a final concentration of 10 μM (Calbiochem) ...
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bioRxiv - Immunology 2021Quote: ... Sequence verified plasmid encoding for mCD22-Fc was transfected together with Flp recombinase-expressing plasmid pOG44 (Invitrogen) using lipofectamine LTX (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS) (Biochron, Berlin, Germany) and 1% penicillin/streptomycin (Invitrogen, Karlsruhe, Germany). Cell cultures were authenticated by short tandem repeat (STR ...
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bioRxiv - Cell Biology 2022Quote: ... and absorbance was measured at 540 nm in a microplate reader (Thermo Scientific™ Multiskan™ FC).
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bioRxiv - Cell Biology 2022Quote: ... Absorbance measurements were performed at 450 nm using a microplate reader (Thermo Scientific™ Multiskan™ FC). For MTT assays ...
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bioRxiv - Cell Biology 2022Quote: ... Nucleolus was stained by incubating with 1:1000 dilution of mouse monoclonal NPM1 antibody (ThermoFisher #FC-61991). Cells were then washed with 1XPBS and fluorescently labeled with goat–anti-rabbit 488 and goat–anti-mouse 594 secondary antibodies (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the cell pellet was resuspended in Fc Receptor Binding Inhibitor Polyclonal Antibody (Invitrogen, 14-9161-73) diluted 1:100 in MACS buffer and incubated for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) and antibiotics (0.1 unit penicillin, 0.1 mg.mL-1 streptomycin, GIBCO) at 37 °C in a humidified 5% CO2 incubator.
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bioRxiv - Microbiology 2022Quote: ... Total plasma from week 11 was purified using CaptureSelect™ IgG-Fc (Multispecies) Affinity Matrix (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and cultivated in DMEM medium (PAA Laboratories GmbH, Germany) containing 10% fetal calf serum (FCS, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Dulbecco’s modified Eagle’s medium (DMEM) and foetal calf serum (FCS) were purchased from GIBCO (Waltham, MA, USA). BCA protein assay kit was purchased from Thermofisher (Waltham ...
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bioRxiv - Biochemistry 2024Quote: Fc fusion proteins were expressed in ExpiCHO cells using the ExpiCHO expression system kit (Thermo Scientific, A29133) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were pre-treated with Fc receptor binding inhibitor polyclonal antibody (eBioscience™, Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Optical density was monitored at 595 nm using a Multiskan™ FC microplate photometer (Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Systems Biology 2023Quote: ... with 100% MetR medium (complete DMEM without L-methionine and L-cysteine, ThermoFisher, 11960-044, 10% FCS). For example ...
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bioRxiv - Immunology 2023Quote: ... The absorbance at 405 nm was measured using a Microplate Photometer (Multiskan™ FC, Thermo Fisher Scientific). To determine specific binding ...
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bioRxiv - Developmental Biology 2023Quote: ... FLs were recovered in Hanks’ balanced-salt solution (HBSS) supplemented with 1% fetal calf serum (FCS) (Gibco), cut in 1 mm2 pieces and incubated with 0,05 mg/ml Liberase TH (Roche ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were resuspended in freezing medium consisting of FCS/40% RPMI 1640 (Thermo Fisher Scientific, cat.: 11875093) + 10% dimethyl sulfoxide (DMSO) ...
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bioRxiv - Biophysics 2023Quote: ... Japan) for confocal imaging and FCS experiments or an 8-well chambered coverglass (155411, Thermo Fisher Scientific) for FLIM experiments ...
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bioRxiv - Microbiology 2023Quote: ... at 1:40,000 dilution for 1 hour at room temperature and visualised on LiCor Odyssey Fc (Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... containing 10% fetal calf serum (FCS) and 0.5% penicillin-streptomycin (pen-strep, catalog number 15140, Life Technologies) at 37°C under 5% CO2 and were passaged twice a week ...
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bioRxiv - Cell Biology 2023Quote: ... The excitation volume (V0) was calibrated by taking FCS data from TetraSpeck™ microspheres (0.1 µm, Invitrogen) of known concentrations.
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bioRxiv - Neuroscience 2023Quote: ... Absorbance of flow-through was quantified using the accuSkan FC plate reader (Fisher Scientific, 14-377-576). Background fluorescence was subtracted and AUC normalized to untreated for each experiment.
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bioRxiv - Immunology 2023Quote: ... The optical density was measured at 450 nm using a microwell plate reader (accuSkan FC; Fisher Scientific). End-point dilution titer was defined as the greatest sera dilution that yielded an OD450 value >2-fold over background.
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bioRxiv - Microbiology 2024Quote: ... Cells were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS) (Gibco) and maintained at 37°C with 5% CO2.
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged Gβγ was expressed in Sf9 cells for 2 days following baculovirus infection (pFastBacDual, Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: His-tagged P-Rex2 was expressed in Sf9 cells for 2 days following baculovirus infection (Bac-to-Bac, Invitrogen). Cells were harvested by centrifugation and lysed by sonication in 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems] ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Biochemistry 2020Quote: ... Purified PCR products were digested with XhoI and NheI and inserted into the pcDNA6/V5-His expression vector (Invitrogen) generating plasmid pMBA40 for POMGNT1 complementation ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were pooled for emulsion PCR using an Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). The enriched samples were loaded onto an Ion PI chip v3 with Ion Chef ...
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bioRxiv - Genomics 2021Quote: ... Sequencing was performed on the Ion Torrent PGM with the Ion PGM Hi-Q View Sequencing kit (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNA-Seq templates were prepared using the Ion PGM Hi-Q View OT2 Kit (Thermo Fisher Scientific Inc.) on an Ion OneTouch 2 system ...
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bioRxiv - Microbiology 2020Quote: 293F cells were transfected by plasmids pOptiVec/V5-His WEAU gp120-trimer using 293fectin according to the manufacturer’s (Invitrogen) instructions ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.