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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... 3 mM L-Glutamine (Gibco, 25030081), 1.5X MEM NEAA (Gibco ...
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bioRxiv - Genetics 2024Quote: ... 3 mM L-Glutamine (Gibco, 25030081), 1.5X MEM non-essential amino acids (NEAA ...
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bioRxiv - Cell Biology 2024Quote: ... and 3 mM L-glutamine (Gibco). MCF-7 cells (ACC115 ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μm particle size (Thermo Scientific) with a 90 minute gradient from 0 to 100% pH 2 solvent B (0.1% formic acid in 80% v/v LC-MS grade acetonitrile) ...
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bioRxiv - Biochemistry 2024Quote: ... using 3 μL Lipofectamine 2000 (Invitrogen). Opti-MEM media was exchanged for DMEM supplemented with 10% FBS but lacking antibiotics for 4-5 hours following transfection ...
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bioRxiv - Microbiology 2024Quote: ... on a QuantStudio 3 (Applied Biosystems), and all primers are listed in Table 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 3 µl GlycoBlue Coprecipitant (Invitrogen), vortexed briefly ...
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bioRxiv - Cell Biology 2024Quote: ... and 3 mM L-glutamine (Gibco).
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Molecular Biology 2024Quote: ... Adult human dermal fibroblast (HDFa) cells (Cascade Biologics C-013-5C) were maintained in Human Fibroblast Expansion Basal Medium (Gibco) supplemented with Low Serum Growth Supplement (Gibco).
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bioRxiv - Microbiology 2023Quote: ... T-293 cells were either transfected with pDEST40-p38-α (5 µg) or with the empty vector (control) using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA). At 24 h post-transfection ...
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bioRxiv - Developmental Biology 2019Quote: Prab-3::GFP was generated by cutting out Prab-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Developmental Biology 2019Quote: Pelt-3::GFP was generated by cutting out Pelt-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 50 nM siRNA (IMPDH2 assay ID: s7417, sense: 5’-CCAAGAAAAUCACUCUUtt-3’; anti-sense: 5’-UUAAGAGUGAUUUUCUUGGtc-3’, Ambion by Life technologies ...
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bioRxiv - Genetics 2022Quote: ... mcherry: 5’-ATGGTGAGCAAGGGCGAGGAG-3’ and 5’-CTTACTTGTACAGCTCGTCCATG-3’) from pKHR8-foxc1aKI and separately subcloned into pJET2.1 (ThermoFisher) to give pJET-mVenus and -mCherry ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...
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bioRxiv - Neuroscience 2024Quote: ... The siRNA sequence for YTHDF2 is 5′-AAGGACGTTCCCAATAGCCAA-3′ and for HIF1α is 5’-GCTGATTTGTGAACCCAT-3’ (Ambion). After 48 h from the initial transfection ...
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bioRxiv - Cell Biology 2019Quote: Cell lines A-375 (ATCC® CRL-1619™) and HEK-293T were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM – Thermo Fisher Scientific, Waltham, MA, EUA) supplemented with fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC, CRL-3216) cells were purchased from ATCC (Manassas, VA, USA) and cultured in DMEM (GIBCO, Thermo Fisher Scientific, Waltham, MA, USA) with 10% FBS (CellMax ...
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bioRxiv - Developmental Biology 2022Quote: ... the coding sequence for the ectodomain of human and zebrafish CACHD1 (truncated before the transmembrane domain at P1095/P1108 respectively) was codon optimised for HEK cells and synthesised by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). These fragments had NotI and AscI target sequences at the 5’ and 3’ ends ...
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bioRxiv - Cell Biology 2024Quote: ... HEK 293T and MEF cells were cultured at 37°C with 5% CO2 in DMEM/F12 medium (Thermo Fisher Scientific – Life technologies #LTI 11039-047) containing 10% fetal bovine serum (Gibco #A3840402) ...
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bioRxiv - Molecular Biology 2024Quote: Signaling via the intracellular Ca++ pathway was assessed in stably transfected HEK- HEK293/hPTH1R/glosensor (GP-2.3) cells using the calcium-sensitive fluorophore Fura2-AM (Invitrogen, Life Tech. Grand Island, NY, USA) [73 ...
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bioRxiv - Microbiology 2020Quote: ... and the Human embryonic kidney (HEK293) cells (Gibco) in Freestyle F17 expression medium (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-human ER(#RM9101,ThermoFisher Scientific, MA). Histological stained slides were scanned using the Aperio ScanScope XT digital slide scanner and positivity analysis for each target gene staining was carried out using Aperio ImageScope V 10.2.1.2314 software ...