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Citations for Thermo Fisher :
4151 - 4200 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Quantification of protein concentration was performed using the Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific, IL, USA). For WB analysis ...
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bioRxiv - Cell Biology 2023Quote: ... rinsed with PBS and ECL performed (SuperSignal West Pico, or SuperSignal West Femto, Thermo Fisher Scientific Inc, IL, USA).
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... The bispecific antibody was purified from the collected supernatant using a protein A column (Thermo Scientific # 89960; IL, USA) and ELISA was used to assess ligand binding affinity and sensitivity of the antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... analysis was performed using TaqMan gene expression assay with ST2 and IL-1RAcP specific primers (Hs00249384_m1 and Hs00895050_m1 respectively, Life Technologies). Normalization of ST2 expression was performed using GADD45a (Hs00169255_m1 ...
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bioRxiv - Genetics 2023Quote: ... as well as editing the PAM site to protect against further edits) (Eurofins, Fisher Scientific LLC, Chicago, IL, USA). The gRNAs ...
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bioRxiv - Genomics 2023Quote: ... The LC-MS/MS system used consisted of an LTQ/Orbitrap-XL mass spectrometer (Thermo Scientific, Rockford, IL, USA) equipped with a Nanoacquity UPLC system (Waters ...
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bioRxiv - Plant Biology 2023Quote: ... Total protein content in the supernatant was estimated using the Pierce BCA protein assay kit (Thermo Scientific, Rockford, IL). The protein samples were denatured at 65 °C in the 1X LDS sample buffer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... following the manufacturer’s protocol (Click-iT HPG Alexa Fluor 488 Protein Synthesis Assay Kit; ThermoFisher Scientific, Rockford, IL, USA). From each sample ...
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bioRxiv - Microbiology 2023Quote: ... USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific, Rockford, IL, USA) at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.6) with 20 mg lysozyme and half of a crushed Pierce Protease Inhibitor Tab (Thermo Scientific, Rockford, IL) and frozen at −20°C ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was concentrated 500-fold using 100 kDA molecular weight cut-off filters (Thermo Scientific Pierce, Rockford, IL) that were centrifuged at 2,500 x g at 4°C until appropriately concentrated ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Cell Biology 2023Quote: ... A stock doxorubicin-HCl solution was initially created by dissolving doxorubicin-HCl (Fisher Scientific, Hanover Park, IL, United States) in DMSO ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels in the supernatant/PBS wash quantified using ELISA kits according to manufacturer’s instructions (murine IL-1β: Invitrogen 88-7013-22 ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Microbiology 2024Quote: ... peptides were analyzed on a Q-Exactive HFX Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Genetics 2021Quote: ... and cytocentrifuged on clean slides (using a Cytospin™ 4 Cytocentrifuge, Thermo Fisher Scientific, at 900 rpm for 4 min). Slides were then immersed in liquid nitrogen for 5 min ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2019Quote: ... post-axotomy cultures were loaded with lipophilic dye N-(3-trimethylammoniumpropyl) -4-(6-(4-(diethylamino) phenyl) hexatrienyl)pyridinium dibromide (FM 5–95; Invitrogen) using KCl mediated depolarization as described previously (Taylor et al ...
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bioRxiv - Physiology 2019Quote: ... 20-30 μ g of protein lysates were denatured in NuPAGE 4× LDS sample buffer and resolved on NuPage 4-12 % Bis-Tris gels (Invitrogen) and the proteins transferred by iBlot (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Microbiology 2021Quote: ... for 20 min at 4 °C and were then centrifuged at 444g for 4 min and dissociated to single cells using StemPro Accutase (Invitrogen). Cells were subsequently fixed using 4% paraformaldehyde (PFA ...
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bioRxiv - Biochemistry 2021Quote: ... 6,8-Difluoro-4-Methylumbelliferyl Phosphate (DiFMUP, #D22065) and 6,8-difluoro-7-hydroxy-4-methylcoumarin (DiFMU, #D6566) were purchased from Life Technologies.
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bioRxiv - Biochemistry 2020Quote: ... The isolated fractions were separated on SDS-PAGE (RunBlue 4-20 %, Expedeon; NuPAGE®Bis-Tris gel 4-12%, Invitrogen) and analysed by InstantBlue staining (Expedeon ...
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bioRxiv - Biochemistry 2021Quote: ... gondii parasites were separated by polyacrylamide gel electrophoresis (PAGE) in precast NuPAGE (4-12% or 12%) or NativePAGE (4-16%) gels (ThermoFisher) according to the manufacturer’s instructions with minor modifications (detailed in the SI) ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Plant Biology 2019Quote: ... 4 µg or less (for samples with <4 µg) of total RNA was reverse transcribed using random hexamers (Thermo scientific), 200 units of Revertaid Reverse Transcriptase (Thermo Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Six hundred μg of the supernatant were incubated for 4 hours under rotation at 4°C with 20 μl of protein A Dynabeads (Invitrogen) containing anti-UNR antibody or rabbit IgG as control in a final volume of 600 μl ...
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bioRxiv - Plant Biology 2021Quote: ... GUS activity was determined by measuring the fluorescence (excitation wavelength 365 nm and emission wavelength 460 nm) of produced 4-Methylumbelliferon (4-MU) in a 96well polystyrene microwell plate (Nunc F96 black flat bottom, ThermoFisher) by the Tecan Infinite 200 PRO microplate reader ...
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bioRxiv - Immunology 2020Quote: ... 4-incubation with 4 μg/mL secondary goat anti rabbit conjugated with AlexaFluor(AF)488 (goat IgG H+L, Invitrogen) in microscopy buffer for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: Total RNA was extracted from DCMA fibroblasts (n=4) and control fibroblasts (n=4) using the RNA extraction Mini kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... 150 ng of total RNA extracted from the brainstem of Vglut2:Ndufs4cKO (n=4) and Vglut2:Ndufs4cCT mice (n=4) was amplified and biotin-labeled using the Illumina TotalPrep RNA Amplification kit (Ambion). 750 ng of the labeled cRNA was hybridized to MouseRef-8 v2 expression beadchips (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... Stained lenses were washed three times in 4°C Medium 199 and then 200 μL 4°C PBS (Gibco, MA) was added to each well prior to measuring DHR fluorescence intensity at Ex/Em of 507/529 nm and Hoescht 33342 at Ex/Em of 360/460 nm with a microplate reader (Spectramax M3 ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of proteins were loaded on either a 4-12% Bis-Tris gel or a 4-20% Tris-Glycine gel (Invitrogen), and SDS gel electrophoresis was performed as previously described (96 ...
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bioRxiv - Neuroscience 2022Quote: Brains were post-fixed overnight at 4’C in 4% PFA then cryoprotected in 30% (w/v) sucrose (Fisher Scientific) solution in PBS for at least 48 hours prior to processing ...
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bioRxiv - Immunology 2022Quote: ... and 125) with TSt-4 (B cells) or TSt-4/DLL1 stromal cells (T cells) in RPMI (Thermo Fisher Scientific) supplemented with 10% BSA (093001 ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... blotted for 4-6 s at 4°C and 100% humidity using a FEI Vitrobot Mark IV (Thermo Fisher Scientific), and plunge frozen in liquid ethane ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Neuroscience 2022Quote: ... and the samples were first postfixed overnight in 4 % PFA (4°C) and then placed in 0.25 M EDTA (Invitrogen AM9261) in PBS at 4°C for 3 d for decalcification ...
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bioRxiv - Systems Biology 2022Quote: ... Proteins were then denatured by incubating samples at 95°C for 5 min in 4 x Laemmli buffer and dithiothreitol (DTT) before loading onto 4 to 12% NuPAGE gradient precast gels (ThermoFisher). Gels were run for 10 min at 110 V ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...