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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: IL-2/Fc fusion proteins were expressed using the Expi293 expression system according to manufacturer instructions (Thermo Scientific). Proteins were as human IgG1 Fc fused at the N-or C-terminus to human IL-2 through a (G4S)4 linker ...
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bioRxiv - Molecular Biology 2021Quote: The peptide concentration was determined by using the Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, IL) and 180 µg peptides from each sample were loaded onto MacroSpin column (NestGroup ...
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bioRxiv - Immunology 2021Quote: ... IL-17A gene expression levels were measured by quantitative RT-PCR with Power SYBR Green technology (Applied Biosystems). Probes were obtained from Applied Biosystems as Assays-on-Demand™ Gene Expression Assays (glyceraldehyde-3-phosphate dehydrogenase GAPDH ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce, Rockford, IL, USA) and analyzed by 10% SDS-PAGE with Coomassie blue staining.
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bioRxiv - Plant Biology 2020Quote: ... followed by addition of 100× diluted secondary antibody: FITC-linked anti-rat IgG (Thermo Fisher Scientific, Rockford, IL) for an additional 1 h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The concentrations of protein in homogenate samples were determined using Pierce BCA protein assay (Thermo Scientific, Rockford, IL).
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bioRxiv - Cell Biology 2021Quote: ... by flow cytometry using an anti-pan cytokeratin AE1/AE3 monoclonal antibody (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Cancer Biology 2021Quote: ... The final detection was performed by enhanced chemiluminescence with SuperSignal West Femto reagent (Thermo Scientific, Rockford, IL, USA). Equal protein loading was confirmed by stripping the blot and re-probing it with 1:1,000 anti-actin antibody.
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bioRxiv - Biochemistry 2020Quote: ... The antigen-antibody complexes were detected using the Pierce ECL chemi-luminescent detection system (Thermo Scientific, Rockford, IL) and visualized on the ChemiDoc XRS+ gel documentation system (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... The HCT116 cells were grown in the Lab-Tek Chambered Coverglass System (154526, Thermo Scientific, Rockford, IL, USA), and the cells were then incubated with 100 nM LysoTracker Deep Red Probe (L12492 ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein concentration for each western blot sample was determined via BSA assay (Thermo Scientific, Rockford, IL, USA). Samples were then equalized and 6x sodium dodecyl sulfate buffer (SDS sample buffer ...
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bioRxiv - Immunology 2022Quote: Cytokine concentrations in culture supernatants were determined using specific ELISA kits (IL-1β and TNFα, Thermo Fisher Scientific) according to instructions provided by the manufacturer.
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bioRxiv - Neuroscience 2022Quote: ... Normal body temperature (36-37°C) was maintained with a circulating warm water blanket (Thermo Scientific, Rockford, IL).
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bioRxiv - Neuroscience 2022Quote: ... and fractionated using the Pierce High-pH Reversed-Phase Peptide Fractionation (HPRP) Kit (Thermo Fisher Scientific, Rockford, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were homogenized for approximately 45 s with a polytron homogenizer (Powergen, Fisher Scientific, and Hanover Park, IL). Throughout homogenization ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... These pieces were digested with 10 ml HBSS containing 2 mg/mL collagenase I (Thermo Scientific, Rockford, IL) for 30 minutes at 37°C with gentle stirring and supernatants were sieved through a 100-μm nylon mesh ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were measured on a quadrupole Orbitrap mass spectrometer (Q Exactive HF, Thermo Fisher Scientific, Rockford, IL, USA) coupled to an EASYnLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Zoology 2020Quote: ... T4 DNA Ligase and JetFlex™ Genomic DNA Purification Kit was obtained from Invitrogen (Thermo Scientific, IL, America). KOD DNA polymerase was purchased from Toyobo ...
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bioRxiv - Cell Biology 2019Quote: ... for 10 minutes, and fixed with methanol:glacial acetic acid, (5:1, v/v) (FisherThermo Fisher Scientific, Rockford, IL). Cells were dropped onto slides ...
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bioRxiv - Biochemistry 2021Quote: ... Protein content was determined by the micro bicinchoninic acid protein assay kit (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... were used for T cell activation and expansion in the condition of human recombinant IL-2 (Gibco, PHC0021) or where indicated ...
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bioRxiv - Biochemistry 2020Quote: Images were recorded using a Titan Krios G3i microscope at University of Tokyo (Thermo Fisher Scientific, Rockford, IL) at 300 keV equipped with a VPP ...
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bioRxiv - Cell Biology 2020Quote: ... Each pooled serum collection was tested for endotoxin using Pierce Chromogenic Endotoxin Quantification Kit (Thermo Scientific, Rockford, IL) and each “batch” used for experiments had undetectable levels of endotoxin.
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bioRxiv - Cell Biology 2019Quote: ... then dissected into individual ovarioles and mounted on slides in Slowfade Gold with DAPI medium (Invitrogen, Rockford, IL). A minimum of two biological replicates were performed for each condition.
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bioRxiv - Bioengineering 2019Quote: ... The concentration of cultivated cells was estimated by OD 600 (NanoDrop 2000c Spectrophotometer, Thermo Scientific, Barrington, IL, USA). DNA was harvested using PureLink® Genomic DNA Mini Kits (Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies used were goat anti-IL-33 (R&D, AF3626) and rat anti-CD8a (Thermo Fisher, 4SM16). Stains were counterstained with hematoxylin using standard methods before dehydrating and mounting.
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bioRxiv - Plant Biology 2019Quote: ... followed by peptide fractionation using the Pierce High pH Reverse-Phase Peptide Fractionation Kit (Thermo Scientific, Rockford, IL). Eight fractions of peptides from each sample were subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... RNA quantity and quality were determined at 260 nm by a Nanodrop-1000 spectrophotometer (Thermo Scientific, Rockford, IL).
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bioRxiv - Developmental Biology 2021Quote: ... filter sterilized using a 5 mL syringe (309646, BD Vacutainer Labware Medical, Fisher Scientific, Hanover Park, IL, USA) with 0.2 µm filter (431219 ...
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bioRxiv - Microbiology 2021Quote: ... Tryptic digestion was performed by adding 0.85 μg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) in 40 μl of ABC to the filters which and incubating for 16 hours in a wet chamber at 37° C ...
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bioRxiv - Immunology 2020Quote: ... Based on the total protein content measured with a NanoDrop™ 2000 spectrophotometer (ThermoFisher Scientific, Rockford, IL, USA), 100 µg or 500 µg of neutrophil proteins was incubated ...
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bioRxiv - Microbiology 2021Quote: ... Cells were pelleted (400xg, 10 min) and supernatants subjected to ELISA analysis for IL-17a (Thermo Fisher, UK).
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bioRxiv - Cell Biology 2022Quote: ... Cell nuclei were counterstained with 0.5 mg/mL DAPI for 5 min (Thermo Fisher Scientific, Rockford, IL, USA) and mounted using Vectashield Vibrance antifade mounting media (Molecular Probes ...
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bioRxiv - Immunology 2022Quote: ... Human IFNg and IL-13 cytokine secretion was measured by ELISA using eBioscience Ready-SET-Go! (now ThermoFisher) ELISA kits according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... CRP and IL-6 were measured in UCB plasma using a high-sensitivity ELISA (Life Technologies, Carlsbad CA) per the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL).
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration was measured by Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Rock ford, IL, USA). The peptides mixtures were clean-up to obtain detergent-free samples with the same kit ...
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bioRxiv - Bioengineering 2024Quote: ... The total EV protein content was measured using a Pierce micro BCA assay kit (Thermo Scientific, Rockford, IL) as described earlier [35 ...
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bioRxiv - Cancer Biology 2024Quote: ... the PBS was aspirated and cells were lysed in complete M-PER buffer (Thermo Scientific, Rockford, IL, USA). Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final ...
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bioRxiv - Neuroscience 2023Quote: Serum concentrations of CRP and IL-6 were determined using a commercially available ELISA kit (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The amount of RNA was quantified using a NanoDrop 1000 spectrophotometer (ND-1000; Thermo Scientific, Rockford, IL, USA). Then to synthesize cDNA ...
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bioRxiv - Genomics 2024Quote: ... while DNA concentration was measured with the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA). The genome was sequenced with both long- and short-read techniques ...
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bioRxiv - Immunology 2023Quote: ... trastuzumab (1.75 mg/mL) was treated with 1/10 volume immobilized papain (Thermo Fisher Scientific, Rockford, IL, USA) in Na3PO4 (20 mM) ...
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bioRxiv - Biophysics 2023Quote: ... The concentration of peptide was determined using Pierce quantitative fluorometric peptide assay (Cat# 23290, Thermo Scientific, Rockford, IL). 11 – 18 μL of the peptides were analyzed through nano-ESI-LC/MS/MS using the Orbitrap Exploris mass spectrometer coupled to a Dionex nano-LC system (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The resulting peptides were subsequently labelled using TMT-11plex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions as indicated in supplementary materials and methods ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 µL of dissolving solution (0.1 g/ml SDS in 0.01 M HCl; Thermo Fisher, Rockfold, IL, USA) was added to each well ...
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bioRxiv - Immunology 2023Quote: Supernatants from restimulated mesenteric lymph node lymphocytes and tissue homogenates were assessed for IL-13(88-7137, Invitrogen), IL-17A (88-7371 ...
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bioRxiv - Plant Biology 2023Quote: ... pH 8.0) and protein concentration was measured using Pierce 660 nm Protein Assay (Thermo Scientific, Rockford, IL, USA) and used for SDS-PAGE and subsequent Immuno-blotting analysis.
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bioRxiv - Physiology 2023Quote: ... and the protein concentration was determined using the Pierce BCA Protein Assays Kit (germo Fisher Scientific, Rockford, IL) Protein samples (25 μg ...