Labshake search
Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... pH 8.0) and protein concentration was measured using Pierce 660 nm Protein Assay (Thermo Scientific, Rockford, IL, USA) and used for SDS-PAGE and subsequent Immuno-blotting analysis.
-
bioRxiv - Immunology 2023Quote: ... trastuzumab (1.75 mg/mL) was treated with 1/10 volume immobilized papain (Thermo Fisher Scientific, Rockford, IL, USA) in Na3PO4 (20 mM) ...
-
bioRxiv - Microbiology 2023Quote: ... diluted 1:2000 in PBST and developed using 3,3’,5,5’-tetramethylbenzidine substrate (Thermo Scientific Pierce, Rockland, IL, USA). Absorbance was measured at 450 nm using an ELISA plate reader.
-
bioRxiv - Physiology 2023Quote: ... and the protein concentration was determined using the Pierce BCA Protein Assays Kit (germo Fisher Scientific, Rockford, IL) Protein samples (25 μg ...
-
bioRxiv - Immunology 2023Quote: ... TNF-α and IL-17 were measured in the culture supernatant using a cytokine measuring kit (Invitrogen, USA) [33].
-
bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
-
bioRxiv - Cancer Biology 2022Quote: ... HepG2 cells were fixed with 4% paraformaldehyde and then stored at 4°C in RPMI 1640 (Gibco, Cat# 11875119) media supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... post-fixed in 4% paraformaldehyde and 30% sucrose for 4 days before being frozen in Neg50 OCT (Thermo Scientific) 30 micron sections were taken and stored in PBS with sodium azide at 4 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... Stock BODIPY505/515 was prepared by dissolving 4,-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene (Life Technologies, D3921) powder into dimethyl sulfoxide (DMSO ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were fixed using a 4% PFA solution [0.2M sucrose | 4% paraformaldehyde solution in PBS (Thermo Fisher, J19943-K2)] ...
-
bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
-
bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
-
bioRxiv - Cell Biology 2019Quote: ... OCT4 and TRA1-60 overnight at 4°C using the pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Life Technologies), followed by incubation with an ALEXA secondary antibodies for 30 minutes at room temperature ...
-
bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system ...
-
bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
-
bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
-
bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
-
bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
-
bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
-
bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
-
bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
-
bioRxiv - Cell Biology 2023Quote: ... were treated either with 0.8 μM CF-4 or control CF-4 (Ctrl-S2-CF4) in DMEM without phenol red (Gibco) and incubated for 10 min at 37°C and 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
-
bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
-
bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
-
bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1,000xg for 4 min and fixed with 4% PFA (Thermo Fisher, Cat. No. 28908) for 15 min on ice ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were probed with mouse anti-HA (12CA5) and mouse anti-Pgk1 (22C5D8, Molecular Probes), followed by probing with mouse anti-Pgk1 (22C5D8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... mouse primary antibody diluted 1:10,000 and anti-mouse-HRP secondary antibody (Thermo Fisher Scientific) diluted 1:50,000.
-
bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 555 Mouse and Alexa Fluor 488 Mouse secondary antibodies were obtained from Invitrogen. The polyclonal antiserum to the recombinant human megalin cytoplasmic domain (anti-megT ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The probes for mouse TNF-α (Mm00443260_g1) and mouse β-actin (Mm00607939_s1) (Thermo Fisher Scientific) were used ...
-
bioRxiv - Microbiology 2022Quote: ... anti-mouse IgG/IgM AlexaFluor 488 and anti-mouse IgG/IgG2α AlexaFluor 546 (both Invitrogen) were used ...
-
bioRxiv - Immunology 2022Quote: ... and anti-pp65 antibody (mouse, Argene) combined with goat anti-mouse antibody (AF488, Thermo Fisher), respectively.
-
bioRxiv - Genetics 2020Quote: ... cells were incubated in secondary antibodies Mouse Texas Red X (ThermoFisher, anti-mouse, 1:500) and Cy5 (anti-rabbit,1:500) ...
-
bioRxiv - Immunology 2020Quote: Mouse serum or plasma was assessed via the IgG (Total) Mouse ELISA Kit (Thermo Fisher), according to the manufacturer’s directions ...
-
bioRxiv - Immunology 2022Quote: ... mouse-anti-mouse/human/rat monoclonal PP1a (1:1000 dilution, number MA5-17239, ThermoFisher Scientific), rabbit-anti-Y.pestis polyclonal YopM (1:1000 dilution ...
-
bioRxiv - Immunology 2023Quote: ... in PBS1X containing MOM reagent (ReadyProbes™ Mouse-on-Mouse IgG Blocking Solution, Invitrogen, R37621) at RT ...
-
bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 350 goat anti-mouse and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen).
-
bioRxiv - Immunology 2024Quote: ... and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were obtained from Thermo Scientific. BHA (Cat# B1253 ...
-
bioRxiv - Cell Biology 2023Quote: ... Alex Fluor 647 goat anti-mouse and Alexa Fluor 594 goat anti-mouse from Invitrogen. All secondary antibodies were used at a concentration of 1:500 ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti 6xHis (mouse monoclonal, HIS.H8, Thermo Fisher Scientific, MA1-21315; WB 1:2000), anti GFP (mouse monoclonal ...
-
bioRxiv - Genomics 2023Quote: ... EY.T434 mouse embryonic fibroblast cells mouse embryonic fibroblast cells were grown in DMEM (ThermoFisher 10566016) supplemented with 15% (vol/vol ...
-
bioRxiv - Cell Biology 2024Quote: ... Alexa 594 goat anti-mouse/rat (1:1000, Invitrogen A-11032 mouse, A-11007 rat) and DAPI (1:500 ...
-
bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
-
bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...