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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 μg biotinylated RBD was pre-coupled with 0.6 ul Streptavidin-AF647 (Invitrogen catalog no. S21374), used as RBD-647 ...
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bioRxiv - Genomics 2022Quote: ... 1:200 dilution of Anti-Mouse SSEA1 Biotinylated antibody (Thermo Fisher Scientific, Cat#: 13-8813-80), 1:200 dilution of Anti-Mouse Gr-1 APC-Cy7 antibody (Biolegend ...
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bioRxiv - Molecular Biology 2022Quote: ... The primary antibody was detected using a biotinylated goat anti-mouse IgG (QD209886, Life Technologies, USA), then an HRP-conjugated streptavidin (ab7403 ...
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bioRxiv - Genomics 2020Quote: ... the biotinylated RNA was resuspended in water and DNAse treatment was performed with TURBO DNAse (Invitrogen) following manufacturer instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The short biotinylated fragment cleaved following nicking was removed by binding to Streptavidin M270 Dynabeads (Invitrogen), and the remaining processed oligos were column-cleaned ...
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bioRxiv - Microbiology 2021Quote: ... cell surface sialic acid residues were oxidised and biotinylated using 1 mM sodium periodate (Thermo Scientific), 100 mM aminooxy-biotin (Biotium Inc. ...
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bioRxiv - Bioengineering 2021Quote: ... Biotinylated PCR products were enriched with Dynabeads™MyOne™ Streptavidin C1 (Thermo Fisher Science, Australia). PCR products on Streptavidin C1 beads were washed with 400 μL 1 × B&W buffer (1 M NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... 160 pmol biotinylated DNA adaptors were conjugated to 400 μg Dynabeads MyOne Streptavidin beads (Invitrogen, 65001) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Purified HPIV3 F was biotinylated using an EZ-link Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher) using a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Cell Biology 2021Quote: 400 pmol of biotinylated RNA was bound to 100 µl Dynabeads® M-280 Strepavidin (Invitrogen) in RNA-binding buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2021Quote: hACE2 and the biotinylated RBDs were produced in 25 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated transcripts were produced from the linearized DNA in vitro using the MEGAscript kit (Life Technologies) and biotin-UTP (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated monoclonal antibodies were detected with APC-or Brilliant Violet 421-conjugated streptavidin (Thermo Fisher Scientific). For intracellular staining of Eomes ...
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bioRxiv - Molecular Biology 2024Quote: ... the purified antigens were then biotinylated with the EZ-Link Sulfo-NHS-Biotin (Thermo Fisher Scientific) using a 50:1 biotin to protein molar ratio for calculations.
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bioRxiv - Biochemistry 2024Quote: ... and biotinylated PDCoV SIL121_2014 ectodomain labeled with both PE and Alexa-Fluor 647 streptavidin (Life Technologies). Sorted IgG+ memory B cells were seeded at clonal dilution in 384-well plates an a monolayer of feeder mesenchymal cells in the presence of B cell survival factors ...
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bioRxiv - Genomics 2024Quote: ... Biotinylated RNAs were enriched with 30 µL of pre-washed Dynabeads MyOne Streptavidin C1 (Life technologies) in binding buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2024Quote: ... hDPP4-Fc and G2 were biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotin (Thermo Scientific) and 80% maximum binding concentrations against S-2P were determined with biotinylated proteins (hDPP4-Fc-biot and G2-biot) ...
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bioRxiv - Immunology 2024Quote: ... the biotinylated ODNs were detected with the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher, 89880) according to the manufacturer’s user guide.
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bioRxiv - Immunology 2023Quote: ... MIF-6xHis was biotinylated using EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher Scientific), performed essentially as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-labeled RNA was then biotinylated with 1mg/ml EZ-link HPDP-Biotin (ThermoFisher, cat: 21341) for 90 min at room temperature as previously described34 ...
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bioRxiv - Immunology 2023Quote: ... These antibodies were biotinylated using the EZ-Link Sulfo-NHS-LC-Biotin Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated antibodies were revealed with Pacific Blue conjugated to avidin (1:1,000; Life Technologies, Paisley, UK). In some cases sections were incubated in biotinylated IB4 (1:500 ...
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bioRxiv - Genomics 2023Quote: ... 400 pmol Biotinylated RNA oligos were conjugated with 50 μl of streptavidin beads (50% slurry; ThermoFisher) in a total volume of 300 μl of RNA-binding buffer (20 mM Tris ...
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bioRxiv - Genetics 2023Quote: ... Religated-biotinylated restriction fragments were pulled down using Dynabeads MyOne Streptavidin C1 magnetic beads (Invitrogen, 65001) according to the manufacture protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... biotinylated fragmented DNA was incubated for 4 hours with streptavidin beads (Dynabeads® kilobaseBINDER™, Invitrogen) to capture only DNA fragments containing the transposon ...
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bioRxiv - Immunology 2023Quote: ... NC and TT were aspecifically biotinylated using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... 10 ng of biotinylated DNA was attached to 4 μl streptavidin-Dynabeads (Invitrogen, catalog number 11205D) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotinylated Tead1 and bound chromatin was pulled down by incubation with streptavidin beads (ThermoFisher Scientific, 11206D). Bead-bound chromatin was resuspended in SDS elution buffer (50 mM Tris–HCl pH 8,10 mM EDTA ...
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bioRxiv - Molecular Biology 2024Quote: ... biotinylated oligoes were stained with 1:200 diluted Alexa FluorTM 568-conjugated streptavidin (Invitrogen™ S11226) incubated in 4% w/v BSA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... heparin was biotinylated by mixing 2 mg heparin and 2 mg amine-PEG3-Biotin (Thermo Scientific), followed by the addition of 10 mg NaCNBH3 ...
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bioRxiv - Genomics 2020Quote: For Hi-C experiments knockdown of CLAMP was validated using the Western Breeze kit (Invitrogen). Antibodies used for detection were a previously described custom rabbit antiCLAMP (1:1,000 ...
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bioRxiv - Genomics 2020Quote: The psep-1::his-58::eGFP transgene was generated using three-site Gateway cloning (Invitrogen) in the MosSCI compatible vector pCFJ150 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10 % heat-inactivated fetal bovine serum (HI FBS, Thermo Fisher Scientific, Loughborough, UK) and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Microbiology 2019Quote: ... A 1:150 dilution of anti-His antibody labelled with AF647 (Thermo Fisher, Waltham, MA) was then added to cells ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Molecular Biology 2019Quote: ... The Jpx E1-E3 isoform was cloned into pEF1/V5-His vector (Invitrogen Cat# V92020), which contains an EF-1α promoter for mammalian expression and a T7 promoter for in vitro transcription ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...
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Genomic approach for conservation and the sustainable management of endangered species of the AmazonbioRxiv - Genomics 2020Quote: ... One microliter of amplification was mixed with 8.5 μL Hi-Di deionized formamide (Applied Biosystems), and 0.5 μL GeneScan 500 LIZ (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved 14His-SUMO and His-Ulp1 were removed by affinity to Ni-NTA Agarose (Invitrogen). The flowthrough was diluted 1:5 with MonoQ buffer A and loaded onto a MonoQ 5/50 GL column (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... under the control of an inducible pBAD promoter (pBAD/His A, Thermofisher, Catalog number 43001) (Supplementary Table 5) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were subcloned into the pAc5.1/V5-His A vector (Invitrogen, V4110-20) with a GFP tag sequence at its 3’ end terminus ...
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bioRxiv - Microbiology 2019Quote: ... with 30% L929 supernatant and 20% fetal bovine serum (HI-FBS, Thermo Scientific, Waltham, MA) for 48 hours at 37°C under 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: His-V5-uL4-CGN mRNAs were obtained using a mMESSAGE mMACHINE T7 kit (Invitrogen #AM1344) using linear DNA templates ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Molecular Biology 2020Quote: ... the reaction pellets were dried and resuspended in Hi-Di formamide (Applied Biosystems/Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... The his-tag was cleaved using AcTEV protease according to manufacturer’s instructions (Invitrogen, Carlsbad, CA), and the his-tagged protease and noncleaved uSpike protein were removed by binding to Ni-NTA resin ...
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bioRxiv - Cell Biology 2019Quote: ... the above mutants are sub-cloned into pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020) with EcoRI and XhoI (New England Biolabs).
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with Alexa Fluor 488–conjugated 6x-His Tag monoclonal antibody (ThermoFisher) for 1 hour at room temperature ...
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bioRxiv - Evolutionary Biology 2019Quote: ... was added to multiplexed product with Hi-Di™ Formamide (Thermo Fisher Scientific, Waltham, MA) to aid in denaturing ...
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bioRxiv - Microbiology 2020Quote: ... All pBAD inducible plasmids generated in this work were derived from pBAD/His A (Invitrogen). pJNS12 is pBAD-gfp-linker-virB flanked by NcoI and HindIII restriction sites ...