Labshake search
Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: The template RNA for cDNA synthesis was isolated from human HEK293 cell culture with TRIzol reagent (Thermo Fisher Scientific, USA), or FFPE samples ...
-
bioRxiv - Immunology 2021Quote: ... The mixtures were kept still for 10 min and then added separately to a 24-well plate with each well containing 1mL of 106/mL HEK293-Freestyle cells (Invitrogen). The transfected cells were cultured by shaking at a speed of 200 rpm at 37 °C for 72 h followed the transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were seeded at ∼10-15 % confluency 48 hours before a triple transfection with Lipofectamine 2000 (Thermo Fisher Scientific) as per the manufacturers protocol.
-
bioRxiv - Systems Biology 2021Quote: ... HEK293 transfected cells were stained with Hoechst 3342 nuclear stain and a wheat germ agglutinin (WGA, Invitrogen Alexa Fluor 647) plasma membrane (PM ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were obtained from ATCC (CRL-1573) and cultured in DMEM with Glutamax and 25 mM glucose (Life Technologies) supplemented with 10% fetal calf serum (FCS ...
-
bioRxiv - Neuroscience 2021Quote: HEK293 HeLa and M17 cells were cultured at 95% air and 5% CO2 in DMEM (for HEK293 and HeLa) or DMEM-F12 (1:1; for M17) supplemented with 10% fetal bovine serum (Gibco) and penicillin-streptomycin (Thermo Fisher) ...
-
Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAsbioRxiv - Molecular Biology 2020Quote: ... The lentivirus of gRNA or shRNA library was produced by co-transfection of library plasmids with two viral packaging plasmids psPAX and pMD2.G into HEK293 cells using Lipofactamine 2000 (Invitrogen). Huh7 cells were transduced with lentivirus libraries at multiplicity of infection (MOI ...
-
bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
-
bioRxiv - Genomics 2022Quote: HEK293 cells were obtained from ATCC (CRL-1573) and grown in DMEM (high glucose, L-glutamine, no sodium pyruvate) (ThermoFisher), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Neuroscience 2024Quote: ... ASAP3 construct was cloned in pcDNA3.1/Puro-CAG plasmid backbone and used to transfect HEK293 cells with Lipofectamine 3000 (ThermoFisher, US) using 400 ng DNA ...
-
bioRxiv - Immunology 2023Quote: ... non-transfected HEK293 cells were labeled with the CFSE CellTrace and the Soromba expressing cells were labeled with Violet CellTrace (Invitrogen). After washing ...
-
bioRxiv - Biochemistry 2024Quote: ... plasmid DNA into 1 × 106 HEK293 cells plated per well of a 6-well plate using Lipofectamine 2000 transfection reagent (Invitrogen). Each well was transfected with 2 µg of total plasmid DNA (50 ng Cg4-puro ...
-
bioRxiv - Bioengineering 2024Quote: Human Embryonic Kidney (HEK293; ATCC Ref: CRL-1573) cells were cultured in Dulbecco’s Modified Eagle Medium + GlutaMAX (Gibco; 10569-010) supplemented with 10% fetal bovine serum (Biowest ...
-
bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney (HEK293) cells (RIKEN Cell Bank, Japan) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, GIBCO, 11965-092) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 3’ ends of the sperm and HEK293 TFAM mRNA species were mapped by 3’ RLM-RACE using the FirstChoice RLM-RACE Kit (Ambion) with modifications ...
-
bioRxiv - Molecular Biology 2022Quote: ... zipper tag via a 5XGGS linker region was co-transfected with N-terminally His-tagged human Leptin in suspension HEK293 FreeStyle cells (ThermoFisher) in the presence of 20 μM kifunensine (Dextra ...
-
bioRxiv - Developmental Biology 2022Quote: Human HEK293 cells were maintained at 37°C in complete media consisting of DMEM with high glucose GlutaMAX Supplement (Gibco) and 10% Fetal Bovine Serum ...
-
bioRxiv - Cell Biology 2023Quote: ... Lentivirus was generated according to the manufacturer’s specifications by co-transfecting HEK293-FT’s with a mixture of packaging plasmids (ViraPower Lentivirus Expression System; ThermoFisher #K497500). Briefly ...
-
bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
-
bioRxiv - Microbiology 2023Quote: ... suspension-adapted and serum-free HEK293S cells were seeded at 3 × 106 cells/mL in Freestyle-293 medium (ThermoFisher Scientific). Next ...
-
bioRxiv - Biochemistry 2023Quote: ... To generate stable expression cell lines Flp-In T-Rex HEK293 were grown in 100 μg/ml Zeocin (Invitrogen, R25001) for at least a week before transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... PANX1 and its mutants and PANX3 were transiently transfected into HEK293 cells with an enhanced green fluorescent protein (eGFP) using the transfection agent Lipofectamine 2000 (Invitrogen). The cells expressing GFP were selected for patch clamp electrophysiological recordings after 24-36 hours of transfection.
-
bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ATG13 KO GFP-LC3B HEK293 cells (a kind gift from O. Florey) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO Life Technologies) containing 10% Fetal Calf Serum (FCS) ...
-
bioRxiv - Biochemistry 2023Quote: U2 OS and HEK293-T cells were grown at 37 °C in 5 % CO2 in DMEM low glucose (Gibco 11885) supplemented with 10 % heat inactivated FBS (F4135 ...
-
bioRxiv - Cell Biology 2023Quote: ... The resulting constructs were integrated into HEK293 Flp-In T-REx cells using the Flp-In technology (Thermo Fisher Scientific). HEK293 Flp-In T-REx were seeded in 6-well plates and co-transfected the following day with 200 ng bait-BirA*-FLAG construct and 2 μg pOG44 (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells stably expressing N-terminally signal sequence FLAG (ssf)-tagged µOR (HEK293-µOR) were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
-
bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293 WT cells were purchased from ATCC (CRL-3216) and maintained in Eagle’s minimum essential medium (EMEM, Fisher Scientific 502382632) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Genetics 2024Quote: HEK293 (XX female) cells and HepG2 cells were cultured in high glucose Dulbecco’s modified eagle medium (DMEM) (41965, Thermo Fisher) and supplemented with 10% fetal bovine serum (10270 ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by a horse radish peroxidase labeled detection antibody (Thermo Scientific #31430) and TMB as substrate ...
-
bioRxiv - Pathology 2021Quote: ... followed by peroxidase-linked donkey anti-mouse IgG (Thermo Fisher Scientific, 45000679) and donkey anti-rabbit IgG (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: ... Species-specific fluorescently labelled infrared (IRDye) and peroxidase-linked (Thermo Fisher Scientific) secondary antibodies were employed as appropriate.
-
bioRxiv - Cell Biology 2020Quote: ... The ImmPACT DAB Peroxidase (HRP) Substrate Kit was obtained from Fisher Scientific. The VECTOR Hematoxylin stain was obtained from Vector Labs ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: The Amplex Red® Hydrogen Peroxide/Peroxidase Assay Kit (A22188; Life Technologies) was employed to detect extracellular H2O2 release ...
-
bioRxiv - Cancer Biology 2022Quote: ... followed by incubation with streptavidin-HRP and TMB peroxidase substrate (Thermo Fisher) to detect binding.
-
bioRxiv - Microbiology 2020Quote: ... Red Hydrogen Peroxide/Peroxidase Assay kit (Molecular Probes, Invitrogen, Burlington, Ontario, Canada).
-
bioRxiv - Biophysics 2020Quote: ... of peroxidase substrate activity (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific). Band intensities were quantified using UN-SCAN-IT gel™ (Silk Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... peroxidase activity was detected with Super-Signal West Pico reagents (Thermo Scientific) using a CCD camera LAS-4000 (Fujifilm) ...
-
bioRxiv - Microbiology 2024Quote: ... or goat anti-rabbit IgG conjugated with peroxidase (Invitrogen 32260, 1:2000). Secondary-probed blots were washed three times with TTBS followed by TBS and incubated with Immobilon chemiluminescent substrate (Millipore) ...
-
bioRxiv - Immunology 2024Quote: ... plates were developed using TMB 1-Component Peroxidase Substrate (Thermo Fisher Scientific), followed by reaction termination using the TMB stop solution (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... IgG3-horse radish peroxidase (HRP) conjugate (1:5000 dilution; Thermo Fisher Scientific) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Peroxidase was revealed with 1-Step ABTS substrate solution (Thermo Fisher, 37615) for 30 min and absorbance at 405nm was measured with a SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 594 Tyramide SuperBoost Kit, Invitrogen, Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... plates were washed and horse-radish peroxidase (HRP)-conjugated secondary IgG (Invitrogen), IgG1 (Invitrogen) ...
-
bioRxiv - Cancer Biology 2023Quote: ... the membrane underwent incubation with peroxidase-conjugated secondary antibodies (Invitrogen, G-21234) for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were visualized by incubation with avidin-biotin-peroxidase complex (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... The whole tag-attached DRD1 was then cloned into the standard pFastBac (Thermo Fisher) vector ...
-
bioRxiv - Developmental Biology 2019Quote: ... which includes both the nuclear H2B-iRFP tag and cell tracker dye (Invitrogen; C12881), Blue and Infra-red fluorescent filter cubes were also used.