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Citations for Thermo Fisher :
4001 - 4050 of 7785 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... DNA was precipitated following the published protocol33 except for the addition of 20 µg linear acrylamide (Invitrogen) instead of glycogen and each fraction was quantified using fluorometry via the Qubit dsDNA High-Sensitivity assay (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... and protein (20 μg, ‘input’) was removed and lysate was added to Protein G Dynabeads (Invitrogen: 10003D) pre-incubated with the appropriate antibody ...
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bioRxiv - Cell Biology 2021Quote: All siRNAs (10-20 nM final concentration) were delivered to the cells by Lipofectamine RNAiMAX (Life Technologies) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and iPSC-CM for 35 minutes with tetramethylrhodamine ethyl ester (TMRE, 20 nM, Invitrogen, Cat# T-669) and Mito Tracker Green (MTG ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed and incubated live for 20 min with 25nM Lysotracker Red DND-99 (ThermoFisher) and Hoecsht 33342 (ThermoFisher ...
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bioRxiv - Biophysics 2021Quote: ... incubated for 20 min at RT with 150 µL solution of 0.2 g/L NeutrAvidin (Thermofisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Compound (Sakura) and cut using a cryotome into sections of 20 μm and collected on SuperFrost (ThermoFisher) cover slides ...
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bioRxiv - Cancer Biology 2021Quote: ... 1xPBS and analysed using a BD LSRFORTESSA X-20 and dead cells were excluded using Hoechst’s (Invitrogen), used at 1:10000 ...
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CULTURE AND MAINTENANCE OF URINE-DERIVED, 3-DIMENSIONAL CANINE TRANSITIONAL CELL CARCINOMA ORGANOIDSbioRxiv - Cancer Biology 2021Quote: ... The remaining cell pellet was then incubated on ice for 20 minutes in Advanced DMEM/F12 (Gibco) + 1mg/mL Penstrep (PS) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 μL of 20 μM stock siRNA was mixed with 5 μL of Lipofectamine RNAiMax (Life Technologies) and diluted in 500 μL of OptiMem (Life Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Mitochondria were labeled by incubating 37 °C for 20 minutes with Mitotracker (200 nM, Thermo Fisher Scientific) before imaging ...
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bioRxiv - Genomics 2022Quote: ... 20 million cells for each condition were then cross-linked with 1% methanol-free formaldehyde (Thermo Fisher) for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting solution were mixed with 20 μL of Dynabeads Protein G for immunoprecipitation (Thermo Fisher Scientific) at 25°C for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... and transfected twice at 0h and 24h with 20 nM of indicated siRNAs using Lipofectamine RNAiMax (Invitrogen). After 24h following the second transfection ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were separated on SDS-PAGE (Novex™ WedgeWell™ 4 to 20%, XP04202BOX, Thermo Fisher) in 1X Tris-Glycine-SDS buffer (BP1341-4 ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were seeded in plating media (10% Fetal bovine serum heat inactivated, 20% dextrose, 1x Glutamax (Invitrogen), 1x penicillin/streptomycin ...
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bioRxiv - Physiology 2022Quote: ... Immunoblots were stripped for 20 minutes with Restore™ Western Blot Stripping Buffer (catalog #: 21059; Thermo Fisher) and re-probed with the following ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were transfected on two consecutive days with 20 nM Cav1 siRNAs using Lipofectamine RNAiMAX (Invitrogen), following the manufacturer’s instructions and recorded 3 days later.
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bioRxiv - Immunology 2022Quote: ... Aliquots of lysate (20 μg protein) were resolved on 4–12% NuPAGE precast gels (Thermo Fisher Scientific) and transferred to PVDF membranes ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction (total volume 20 μL) consisted of Master Mix (10 μL, 2X; Thermo Fisher Scientific, USA), bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... with 10% (or 20% for CA46 and SET-2 cells) FBS (Thermo Fisher Scientific, #10-437-028) at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... We run 20 μg total proteins on 4-12% NuPAGE Bis-Tris mini protein gels (Invitrogen; NP0321BOX). Proteins separated by their approximate sizes on the gel were then transferred onto nitro-cellular membrane (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... in 20 μl reactions with the Fast SYBR™ Green Master Mix (Applied Biosystems™, cat.no.4385616), primers (Table S1a ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μl of cells at OD600 ∼ 0.05 were mixed with 20 μM Propidium Iodide (P3566, Molecular Probes) and plated in half-area ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA plus PIC) with 20 mM thiol-reactive methyl-methane thiosulfonate (MMTS, Thermo Fisher Scientific) to block free cysteine residues ...
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bioRxiv - Neuroscience 2020Quote: ... 20 μg of protein was resolved by SDS-PAGE on 4-12% Bis-Tris mini-gels (Invitrogen). Separated proteins were transferred to nitrocellulose membranes and blocked in 5% milk/TBS-0.1% Tween for 2 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... For detection of effector caspase activity 20 µM CellEvent Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific) was added to the infected cells prior to imaging ...
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bioRxiv - Microbiology 2021Quote: ... The 20 µl reaction volume consisted of 5 µl Taqman Fast Advanced Mastermix 4x (Thermo Fisher Scientific), 0.4 μl of tat 2.0 forward primer and rev reverse primer (each at 20 µM ...
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bioRxiv - Molecular Biology 2020Quote: ... and 20 µg of protein were boiled in the presence of NuPAGE LDS sample buffer (Invitrogen NP0007) and NuPAGE Sample Reducing Agent (Invitrogen NU0004 ...
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bioRxiv - Molecular Biology 2020Quote: ... The 20-μl PCR mixtures contained 1x Dream Taq buffer with 2 mM MgCl2 (Thermo Scientific, USA), 0.25 mM dNTPs mix ...
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bioRxiv - Neuroscience 2021Quote: ... NaCl 10 mM, MgCl2 3 mM, Tween-20 0,1%, Nonidet P40 Substitute 0.1%, Digitonin 0.01%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0.04 U/μL) ...
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bioRxiv - Immunology 2020Quote: The qPCR amplification reactions totaled 20 µL that included Power SYBR Green PCR Master Mix (Applied Biosystems), 0.5 µM forward and reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... and samples were prepared for SDS-PAGE (20 ug per sample) in SDS sample buffer (Life Technologies) and boiled at 95°C for 10 min before electrophoresis on 4-12% pre-cast gels (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... 10-20 μL of cell suspension was spread onto pre-cleaned fluorescent antibody slides (ThermoFisher, 3032-002) coated with poly-L-lysine (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with a C18 Acclaim Pepmap100 preconcentration column (100 µm i.D. x 20 mm, Thermo Fisher Scientific) and an Acclaim Pepmap100 C18 nano column (75 µm i.d ...
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bioRxiv - Molecular Biology 2020Quote: The qPCR reactions were performed in 20 μL comprising 1x TaqMan Universal PCR mastermix (Applied Biosystems, USA), optimal concentrations of forward and reverse primers ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of 20 mg/mL RNAse A and 1 U/μL DNAse (Invitrogen, Carlsbad, CA, USA) were added to 1 mL of the filtered lysates to limit contamination by host nucleic acids ...
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bioRxiv - Biophysics 2020Quote: ... 50 mM NaCl) followed by a 2 min incubation of 20 µL streptavidin (0.1 mg/mL, ThermoFisher). Excess streptavidin was removed with 100 µL T50 ...
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bioRxiv - Microbiology 2021Quote: ... sheared chromatin was precleared for 20 min at 4’C using Protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitations were performed using fresh beads and anti-Histone H3 acetyl K27 (H3K27Ac ...
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bioRxiv - Molecular Biology 2022Quote: ... or 20 μL of Dynabeads Pan Mouse IgG (Invitrogen 11041, for Ddi1-TAP and Ubx5-TAP ChIP) overnight at 4 °C with rotation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific; 4346906). qPCR was carried out on a real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... for a total reaction volume of 20 μl in 96-well PCR plates (Thermo Fisher Scientific; 4346906). qRT-PCR was carried out on a real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... BCSCs or xenograft-derived tumor cells were labeled with 20 µM cell proliferation dye eFluor450 (Thermo Fisher). 1x105 tumor cells and 1x105 γδ T cells were co-cultured for 3 h at 37°C in the presence of 1 µl α-CD107a-PE antibody (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... plates were washed 4 times with PBS/0.05% Tween-20 and blocked using Superblock (Thermo Fisher Scientific) for 1.5 hours at 37°C ...
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bioRxiv - Systems Biology 2022Quote: For culturing we used 20 mm x 150 mm glass test tubes (Fisher Scientific, Hampton, NH, USA) with disposable ...
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bioRxiv - Biophysics 2022Quote: ... and frozen at −20 °C until analysis on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen). The gels were washed in water ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 20 µl of CD3/CD28 Dynabeads (Thermo Fisher Scientific, 11131-D) per million cells ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative RT-PCR was performed in 20 µL reaction volumes containing 1X TaqMan Gene Expression (Applied Biosystems) Assay ...
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bioRxiv - Cell Biology 2020Quote: Guts (15–20) were quickly dissected in cold 1× PBS and transferred to TRIzol Reagent (#15596026, Invitrogen). RNA extractions were performed as described in the TRIzol Reagent protocol ...