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Citations for Thermo Fisher :
3951 - 4000 of 7785 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... fly heads (10-20 per biological sample) were homogenised in 1x NuPAGE LDS sample buffer (Thermo Fisher) with 200 mM DTT (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse transcription was performed in a final volume of 20 µL using Superscript IV (Thermofisher scientific) and Random hexamers on either 1-2 µg of total RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... and subjected to electrophoresis on a Novex™ 4-20% Tris-Glycine Mini Gel (Thermo Fisher Scientific). The protein bands were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... For qPCR 5–20 ng cDNA was mixed with the Fast SYBR Green Master mix (Applied Biosystems) and amplified with a Light-cycler (Roche) ...
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bioRxiv - Genomics 2020Quote: ... Protein was degraded by the addition of 5 μL of 20 mg/mL proteinase K (Invitrogen 25530049) and incubation at 50°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and 20 μL of the supernatant was added to 180 μL of Coomassie Plus (Thermo Scientific #23238). Absorbance measurements were taken at 595 nm in triplicate ...
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bioRxiv - Developmental Biology 2020Quote: ... slides were washed in PBST (0.1% Tween 20 in PBS) and incubated with appropriate secondary antibodies (Invitrogen) at 1:200 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS for 20 mins at room temperature (RT) and blocked with 10% goat serum (Life Technologies) for 20 mins at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were blocked with 5% milk in PBS supplemented with 1µl/ml Tween-20 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Physiology 2021Quote: ... Equal amounts of total proteins (10 - 20 μg) were mixed with NuPAGE LDS Sample Buffer (Thermo Fisher) with 100 mM of DTT and separated by electrophoreses on NuPage 4%–12% Bis-Tris gels and transferred to PVDF membranes ...
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bioRxiv - Genetics 2021Quote: 20-50 mg of tissue or cells were homogenized in 1 ml of Trizol (ThermoFisher Cat # 15596026) according to the manufacturer’s protocol in a TissueRuptor (Qiagen Cat # 9002755 ...
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bioRxiv - Genetics 2020Quote: ... Approximately 20 mg of ground gall tissue was suspended in 100 µL methanol (Optima grade, Fisher Scientific) and 400 µl of 5% aqueous formic acid (Optima grade ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we first extracted RNA from 20 pooled heads for Y-replacement line males using TRIzol (Invitrogen #15596026). We then made libraries using the Illumina TruSeq Stranded Total RNA Ribo-Zero Gold kit (Illumina RS-122-2201).
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bioRxiv - Cell Biology 2021Quote: Mitochondrial membrane potential was tested using the fluorescent potentiometric compound tetramethylrhodamine methyl ester (TMRM, 20 nM; Invitrogen). Cells were incubated in DMEM without phenol red with 0.1% FBS and CsH (1 µM ...
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bioRxiv - Immunology 2021Quote: ... and blocked in PBS + 0.1% Tween-20 + 5% bovine serum albumin (BSA) (Sigma-Aldrich or Fisher Scientific). If needed ...
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bioRxiv - Immunology 2021Quote: ... sagittal sections of 20 µm from frozen brain tissue were transferred to SuperFrost Plus™ (Thermo Scientific) slides ...
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bioRxiv - Immunology 2021Quote: ... for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher, W32464), donkey anti-rabbit-AF488 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher, W32464), donkey anti-rabbit-AF488 (Abcam ...
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bioRxiv - Plant Biology 2020Quote: ... Then 20 μL 10X DNase I buffer and 2 μL RNase-free DNase I (Ambion; http://www.lifetechnologies.com) were added to each tube ...
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bioRxiv - Neuroscience 2020Quote: ... for 20 minutes at room temperature and mounted with ProLong Gold Antifade Mountant with DAPI (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: ... 20 μg of the supernatant were separated by 4–12% NuPage Bis-Tris PAGE (Thermo Fisher Scientific) and transferred to membranes using the iBlot nitrocellulose membrane Blotting system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... The minced tissue was suspended in 20 ml of 1X Accutase Cell Detachment Solution (Thermo Fisher Scientific) and placed to rotate at 37 degrees Celsius for 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... For data acquisition grids were imaged on a FEI Tecnai G2 20 (Thermo Fisher Scientific, The Netherlands) operated at 200 kV using a 200 μm condenser lens aperture and 100 μm objective lens aperture ...
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bioRxiv - Biochemistry 2020Quote: ... coverslips were clicked for 20 minutes using a commercial kit (Click-iT Cell Reaction Buffer Kit; ThermoFisher), with 3 mM AlexaFluor 647-azide (ThermoFisher) ...
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bioRxiv - Bioengineering 2021Quote: ... Adherent cells were then incubated for 20-30 minutes with a 1X ALP Live Stain solution (ThermoFisher). After incubation ...
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bioRxiv - Bioengineering 2020Quote: ... and incubated with 100 µl Streptavidin-HRP (20 ng/ml in PBS-BSA 1%, 21134; Thermo Scientific) for 30 min at RT ...
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bioRxiv - Biochemistry 2021Quote: ... and combined with 20 μl of a solution containing 10 μl of 5x First-Strand buffer (Invitrogen), 2.5 μl of 10 mM dNTP mix (NEB) ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate (25% of total) was loaded into a 4-20% Tris-Glycine gel (Invitrogen Cat# XP04200BOX), run in Tris-Glycine SDS Running buffer (Novex Life technologies Cat# LC2675 ...
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bioRxiv - Neuroscience 2021Quote: ... Dead cells were labeled using Violet fluorescence reactive dye for 20 min (1:1000, ThermoFisher, Cat# L34963). Cells were fixed for 15 min using BD Cytofix/Cytoperm reagent (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins (20 μg) were separated by using 12% bis-tris −SDS-PAGE (NuPAGE, Invitrogen, Carlsbad CA, USA) by electrophoresis ...
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bioRxiv - Neuroscience 2021Quote: ... EBs were transferred onto Poly-L-Ornithine (Sigma P4957-50ML; 20 μg/ml in sterile water Gibco 10977035 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was quantified and reverse transcribed using oligo(dT)20 primers and the SuperScript IV kit (ThermoFisher). RT-qPCR was performed on the LightCycler 480 II instrument (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... 6xHis-SNAP-Rab5B was then captured onto 20 μL Dynabeads His-Tag Magnetic Beads (Thermo Fisher Scientific) and washed three times in Ni-binding buffer in 2 mL Protein Lo Bind Tubes (Eppendorf) ...
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bioRxiv - Cell Biology 2021Quote: Cells starved for 30 min in serum-free medium supplemented with HEPES (20 mM, pH 7.4; Invitrogen) and 0.5 % (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µm sagittal sections of fresh frozen brainstems were cut on a cryostat (CryoStar NX70, Thermo Scientific), mounted on SuperFrost Plus (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... was incubated for one hour with 20 μL Dynabeads Protein G magnetic beads (Thermo Fisher Scientific, 10004D), washed ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 to 20 μg of protein were boiled in 1x LDS Non-Reducing Sample Buffer (Life Technologies) and supplemented with 5% 2-mercaptoethanol (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... 20 µg of protein were separated on Bolt 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were rinsed in PBS 4 × 20 minutes and then incubated in Hoechst (1:2000; Thermo Fisher) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then run on a reducing Novex 10-20% Tris-Glycine gel (Thermo Fisher Scientific) and stained by Coomassie blue as described above ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... from 3% acetonitrile to 20% acetonitrile in 100 minutes into an Orbitrap Fusion mass spectrometer (Thermo Scientific). Precursor mass spectra (MS1 ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... from 3% acetonitrile to 20% acetonitrile in 100 minutes into an Orbitrap Fusion mass spectrometer (Thermo Scientific). Precursor mass spectrums (MS1 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were trypsinized and resuspended in HBSS containing 20 uM H2DCFDA (Thermo Fisher Scientific, Cat# D-399). Cells were incubated in a 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and for an additional hour with 20 µl Protein A/G Agarose beads (Thermo Fisher Scientific, USA) by gentle rotation at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The 20 μL reactions consisted of Applied Biosystems® TaqMan™ Environmental Master Mix 2.0 (ThermoFisher Scientific), 300nM each T ...
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bioRxiv - Neuroscience 2020Quote: ... Aliquots of protein (20-40 μg) were separated using Bolt Bis-Tris gels (4-12%; Thermo Scientific) and transferred to a nitrocellulose membrane which was probed using appropriate primary and secondary antibody pairs ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed by adding 20% neutral-buffered formalin (NBF) (Thermo Scientific #23-751-800, Kalamazoo, MI) directly to the media for 30 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 20 µl isolated RNA was treated with DNase using the DNA-free Kit (Ambion, Carlsbad, CA, USA). RNA quality and quantity were determined on the Agilent 2100 Bioanalyzer platform (RNA 6000 Nano ...
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bioRxiv - Physiology 2021Quote: ... 20 µg of protein samples were loaded on a NuPage gel (4-12% polyacrylamide, catalog #NP0321, Invitrogen) and separated by SDS-PAGE at 160 V ...