Labshake search
Citations for Thermo Fisher :
3951 - 4000 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: African green monkey kidney cells (COS-7) were cultured at 37°C and 5 % CO2 in DMEM medium containing glutamax (Gibco No. 31966-047), 10 % fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2022Quote: ... and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies; Supplementary Table 1). The relative gene expression was calculated using the 2-ΔΔCt quantification method after normalization to GAPDH values and expressed as fold of levels found in undifferentiated hPSCs cells for cultured cell or in WT mice group for liver analysis.
-
bioRxiv - Microbiology 2022Quote: ... The eukaryotic cells were mechanically lysed by vortexing the 15 mL conical tube on setting 7 using a Pulsing Vortex Mixer (Fisher Scientific Cat # 02215375), with two 30 second pulses and 30 second ice incubations after each pulse ...
-
bioRxiv - Plant Biology 2022Quote: ... Afterwards the gels were incubated in SyproRuby fixation solution (50% MeOH, 7% acetic acid) for 30 min and incubated overnight in SyproRuby staining solution (Thermo Fisher Scientific - S12000). The following day the gels were washed applying SyproRuby washing solution (10% MeOH ...
-
bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... 7 mM (ATP) and 100 mM (Na+) before vitrification by plunge freezing using an Vitrobot Mark IV (Thermo Fisher Scientific, Hillsboro, Oregon, USA) onto Quantifoil R1.2/1.3 copper grids (Quantifoil Micro Tools GmbH ...
-
bioRxiv - Genetics 2024Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...
-
bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides, Thermo Scientific): [Biotin-7-dATP ...
-
bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
-
bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
-
bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
-
bioRxiv - Cancer Biology 2022Quote: ... HepG2 or MCF-7 cultures were grown for imaging on poly-D-Lysine coated 8-well LabTek II chambered coverglasses (Nunc, Rochester, NY, USA) for 1-2 days ...
-
bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
-
bioRxiv - Cancer Biology 2023Quote: ... fresh sections of each FFPE block were cut on a microtome at 7 μm thickness and mounted on glass slides with polyethylene naphthalate membranes (Thermo Fisher Scientific, LCM0522) and stored in a nitrogen chamber ...
-
bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
-
bioRxiv - Biochemistry 2023Quote: ... at ~10 mg ml-1 concentration and blotted for 5-7 s followed by plunge-freezing into liquid ethane using a Vitrobot MarkIV (Thermo Fisher Scientific, USA) operating at 100 % humidity ...
-
bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
-
bioRxiv - Genetics 2023Quote: ... Blocks were later cut on a cryostat into 7-10 µm sections and placed on Fisherbrand Tissue Path Superfrost Plus Gold slides (Fisher Scientific; Cat# FT4981gplus). H&E staining was performed as previously described(Beedle 2016) ...
-
bioRxiv - Biochemistry 2023Quote: ... and transferred onto a 0.45 μm nitrocellulose membrane for 7 min at 20 V with the i-Blot 2 (Thermo Fisher, Waltham, MA, USA). Blocking ...
-
bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
-
bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
-
bioRxiv - Immunology 2023Quote: ... qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific; Oakville, Canada). All cycle threshold (CT ...
-
bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
-
bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2023Quote: The number of epithelial cells in the Cervix Chip was obtained by digesting the Cervix Chip apical channel at day 7 of differentiation or day 3 on co-culture with bacteria with Collagenase IV (Gibco, Cat. No. 17104019) in TrypLE (Thermo Fisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... referred to as ENAS medium (supplementary table 1) PDTs were routinely passaged at intervals of 7 days using TrypLE (Gibco, Cat. No. 12605010) and pipetting to dissociate the cells.
-
bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged for 3 min at 500 g and Accutase was replaced with 500ul assay medium containing 2μM 2’,7’ –dichlorofluorescein diacetate (DCFDA) (Thermo Fisher Scientific, cat.no. D399) and 1:1000 live-dead stain Zombie NIR (Biolegend ...
-
bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids listed in Supplementary Table 7 into Expi293F cells was performed according to the manufacturer’s instructions using the Gibco™ ExpiFectamine™ 293 Transfection Kit (Gibco™ A14524). Following transfection ...
-
bioRxiv - Neuroscience 2024Quote: Real-Time RT-PCR reactions were performed in a QuantStudio 7 Flex Real-Time PCR System using SYBR green technology (PowerTrack SYBR Green Master Mix, Thermo Fisher Scientific, A46109), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: Human embryonic kidney cell line HEK293FT and breast cancer cell line MCF-7 were both cultivated in Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, Gibco) supplemented with 10% FCS (fetal calf serum ...
-
Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defectsbioRxiv - Developmental Biology 2024Quote: ... Insoluble proteins and other debris were removed by centrifugation at 12000 rpm for 7 minutes in a microcentrifuge and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, USA) according to the manufacturer’s protocol.
-
bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml of recombinant trimeric S protein (50ul per well ...
-
bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 µg/ml of recombinant trimeric S protein (50 µL per well ...
-
bioRxiv - Biophysics 2021Quote: ... The AutoScript 4 Software is commercially available from Thermo Fisher Scientific.
-
bioRxiv - Biochemistry 2020Quote: SDS-PAGE electrophoresis (4-12% Bis-Tris gel; Life Technologies) demonstrating Ni-NTA purification of nsp10 and nsp16 ...
-
bioRxiv - Cancer Biology 2021Quote: ... followed by 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) staining for 10 min ...
-
bioRxiv - Cell Biology 2020Quote: ... target cells were selected using 4 μg/ml puromycin (ThermoFisher) or 150 μg/ml zeocin (Invivogen ...
-
bioRxiv - Cell Biology 2020Quote: ... 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, D1306) was utilized to detect the nuclei ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
-
bioRxiv - Immunology 2021Quote: ... and were loaded on a 4-12% NuPAGE gel (Invitrogen). Electrophoresis was performed in an MES buffer at 200V for 35 min ...
-
bioRxiv - Cell Biology 2019Quote: ... or 4 mL diamidino-2-phenylindole (DAPI; Thermo Scientific, 62248) in DPBS at a 1:1000 concentration ...
-
bioRxiv - Immunology 2021Quote: ... NK-92 cells were loaded with Fluo-4 NW (Invitrogen) according to the manufacturer’s protocols ...
-
bioRxiv - Immunology 2021Quote: ... Right after centrifuging from the cytocentrifuge (Cytospin 4, Thermo Scientific), fresh BAL cell cytospin slides were stained using a Kwik-Dif Stains kit (Fisher Scientific ...
-
bioRxiv - Physiology 2020Quote: ... and run on 4-12% gradient Tris-glycine gels (Invitrogen). The proteins were transferred onto a nitrocellulose membrane (Thermo Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... 96-well flat bottom Immulon 4 HBX plates (ThermoFisher Scientific) were coated with 150 μl/well of fetuin (Sigma Aldrich ...
-
bioRxiv - Developmental Biology 2021Quote: ... embryos were collected and fixed in 4% paraformaldehyde (Fisher Scientific) overnight before being processed through graded ethanols and xylene and then embedded in paraffin as previously described (Tavares et al. ...
-
bioRxiv - Cell Biology 2022Quote: ... and resolved using NuPAGE 4-12% Bis-Tris gels (Invitrogen). Proteins were transferred to PVDF membranes and immunoblotted ...
-
bioRxiv - Neuroscience 2021Quote: ... cryoprotected at 4°C in 30% sucrose (Thermo Fisher Scientific) for 48 h ...
-
bioRxiv - Neuroscience 2021Quote: ... BMEs were labeled with FM 4-64 (Molecular Probes, Inc.) or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences ...
-
bioRxiv - Molecular Biology 2020Quote: ... Hive Five cells (BTI-TN-5B1-4) (Thermo Fisher Scientific) were cultured in ESF921 medium (Expression Systems ...