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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were plated with antibiotic-free medium at a density of 7 × 106 cells in a T75 flask and transfected via Lipofectamine3000 (Invitrogen, Carlsbad, CA) using a total of 15 µg of BEI lentiviral plasmids (Table 1) ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cancer Biology 2023Quote: ... then plated in duplicate in 7 μL medium at 125 cells per well into compound-containing wells using a Multidrop Combi Reagent Dispenser (Thermo Scientific 5840320) plate dispenser ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 3,000 MCF-7 cells and 10,000 HCC1500 cells were seeded in black 96-well optical bottom plates (Thermo Scientific™, CAT: 165305) in 100 µL per well ...
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bioRxiv - Immunology 2023Quote: ... diluted to 2 μM in warm RPMI-1640 and incubated at 37°C for 30 min before washed and stained with 7-AAD (Invitrogen, cat # A1310).
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with antibody cocktail (Supplementary Table 7) along with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen; cat # L34957) or eBioscience Fixable eFluor780 viability dye (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cos-7 cells (ATCC) were cultured in DMEM supplemented with 10% FBS and 100 U/ml penicillin/streptomycin (all Thermo Fisher Scientific) in 10 cm culture dishes ...
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bioRxiv - Bioengineering 2022Quote: The level of reactive oxygen species was assessed in the homogenate supernatant of RPE and retina tissue with using 2′–7′-dichlorofluorescein diacetate (DCFH-DA; ThermoFisher Scientific Inc.) molecular probes ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed on a LifeTechnologies QuantStudio 7 real-time PCR system using SYBR™ Green PCR Master Mix (Applied Biosystems). Gene expression levels were normalized to Actb or Gapdh ...
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bioRxiv - Cancer Biology 2023Quote: siRNA screening was performed in MCF-7 TEAD reporter cells using two different siRNA libraries (Dharmacon On-Target Plus and Ambion Silencer Select) targeting ubiquitin-related genes ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems. The layout of the PCR array 96-well plate consisted of probes/primers for 84 inflammasome-associated genes ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantStudioTM Software by Applied Biosystems. Minus reverse transcriptase and no-template cDNA controls were included ...
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM MgSO4 pH 7 with KOH) and root tip dissection on polylysine-coated Epredia® SuperFrost Ultra Plus Gold slides (ThermoFisher Scientific), the slides with the root samples were mounted under a counter slide using 1X MTSB solution and positioned in the InsituPro VSi holder ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted from 40 pairs of adult testes per sample per condition (3 to 7 days post-eclosion) using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells (ATCC CRL6509) and COS-7 cells (UVA Tissue Culture Facility) were maintained in DMEM with glucose and GlutaMAX (Gibco 10566-016) plus 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... At the end of each qPCR amplifications plots and melt curves (ΔRn vs cycle per well) were automatically calculated by the Quant Studio 7 Pro qPCR instrument (Thermo Fisher Scientific). Relative circRNA levels were quantified directly from the Ct average’s as shown before.17,20
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bioRxiv - Cancer Biology 2024Quote: ... Clonal gp130 KO Huh-7 cells were transduced with this vector and selected and subsequently cultured with neomycin (400 µg/ml; Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Clonal gp130 KO Huh-7 cells were transduced with this mutant vector and selected and subsequently cultured with neomycin (400 µg/ml; Thermo Fisher Scientific). Short hairpin (sh)RNA knockdown constructs were acquired from the Mission TRC shRNA library (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR analysis was performed using Green PCR Kit (TransGen, Cat# 2-21) on a QuantStudio 7 Flex system (Thermo fisher, USA). The gene-specific primers were listed in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... mononucleated cells were sorted as described in “Fluorescence-activated MuSC sorting and culturing” part with 7-AAD (Thermo Scientific, 00-6993-50) staining for viability selection ...
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bioRxiv - Bioengineering 2024Quote: ... The reaction was incubated for 30 minutes at 4 °C with mixing then the periodate was removed on a Zeba 7 kDa molecular weight cut-off spin desalting column (Thermo Fisher Scientific) with a mobile phase of PBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were cut at 7 µm thickness and mounted onto pre-charged slides (#12-550-15 Superfrost Plus slides, Fisher Scientific, USA). For immuno-fluorescence (IF) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We quantified the libraries again using Qubit and qPCR (Kapa qPCR reagents; ViiA 7 Real-Time PCR System, Thermo Fisher Scientific Inc.) and grouped them into pools (4–10 libraries/pool ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were minced in 2 mm sections using a razor blade in ice-cold B1 buffer (Hanks Balanced Salt Solution with 10 mM HEPES, pH 7; Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Genetics 2021Quote: ... the 700 bp PCR products were subcloned into the pCR™4-TOPO® TA vector and transfected into Escherichia coli DH5α cells (TOPO 4 Kit, ThermoFisher Scientific) followed by plasmid preparation and direct Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Developmental Biology 2020Quote: Transfection mix was prepared by combining PEI and plasmid DNA (4:1 ratio; 4 µg PEI per 1 µg DNA) in Opti-MEM™ Reduced Serum Medium (Thermo Fisher Scientific) followed by brief vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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bioRxiv - Microbiology 2022Quote: ... PAFB was labelled with the green fluorophore 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-propionyl ethylenediamine hydrochloride (BODIPY™ FL EDA, Invitrogen, Waltham, MA, USA) as described (32).
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bioRxiv - Genetics 2022Quote: ... The styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64, 514/670 nm absorption/emission Invitrogen™, Waltham, Massachusetts) was used at a final concentration of 16.5 μM in ddH2O from a 16.5 mM stock solution in DMSO ...
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Neuroscience 2022Quote: ... Slices were quickly fixed overnight (ON) in 1x PBS pH7.4, 4% sucrose (Serva, Heidelberg, Germany) and 4% PFA (Paraformaldehyde; Thermo Fisher Scientific, Waltham, USA). Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... cells were centrifuged (300rcf, 7min, 4°C) and fixed (1h, 4°C) using the eBioscience Foxp3/Transcription factor staining buffer set (ThermoFisher #00-5523-00) fixation/permeabilization reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...
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bioRxiv - Pathology 2023Quote: ... Sections were cut (4 µm) using a cryostat and labelled overnight at 4°C with COL6 antibody (1:500) (MA5-32412, Thermo Fisher Scientific, USA) after blocking nonspecific binding sites with 10% bovine serum albumin (A7906 ...
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bioRxiv - Bioengineering 2023Quote: ... The staining of MyHC was performed by incubating the samples for 4 hours with the mouse anti-MyHC antibody (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™), which was used at a 1:10 dilution for a final concentration of 10 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The grids were automatically blotted for 4 s at 4°C and 100% humidity and flash-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). Cryo-EM data were collected using a 300 kV Titan Krios G2 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... A droplet of 2.5 μL of purified NaV1.6EM complex was applied to the grid followed by blotting for 4-5s at 4°C under 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). In the case of the preparation of NaV1.6EM complex with 4,9-anhydro-TTX ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 4 μg of plasmid per 4 million cells using the lipofectamine 2000 reagent and protocol (Thermo Fisher Cat# 11668019). Nuclear extraction was performed 24 hours after transfection utilizing nuclear extraction kit from Abcam (ab113474) ...
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bioRxiv - Cell Biology 2024Quote: ... fixed in 4% PFA for 15 minutes at 4 °C and incubated in 1X InVitrogen eBioscience Cell Permeabilization buffer (InVitrogen 00-8333-56) for 15 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and coverslips were washed with 1X PBS and fixed with cold 4% PFA/PBS pH 7.4 (Paraformaldehyde Solution, 4% in PBS, Thermo Scientific™, #J19943.K2) for 15 minutes at RT ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We used para-chlorophenylalanine (PCPA, a.k.a. DL-4 Chlorophenylalanine, fenclonine) (CAS: 7424-00-2, ThermoFischer, Acros Organics, Geel, Belgium; stored at 4°C), an inhibitor of the enzyme tryptophan hydroxylase (TPH) ...
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bioRxiv - Neuroscience 2019Quote: ... cycling synaptic vesicles were stained with the styryl dye N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino) styryl) pyridium dibromide (FM1-43; Molecular Probes, Inc., Eugene, OR). For labeling of synaptic vesicles ...