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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... hiPSC-CMs were quickly washed with DPBS and incubated with SARS-CoV-2 at 5 multiplicity of infection (MOI) diluted in DMEM only (Gibco) for 1 h at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 E sgmRNA standards were generated by in-vitro transcription of the generated SARS-CoV-2 E sgmRNA cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Microbiology 2022Quote: ... BHK-21 were transfected to express the SARS-CoV-2 S protein using Lipofectamine 3000 (Thermo Fisher Scientific, Madrid, Spain) and after 24h transfected cells were inoculated with a replication-deficient rVSV-luc pseudotype (MOI ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA mini kit (Invitrogen) and subjected to cDNA synthesis using a PrimeScript RT reagent kit (Perfect Real Time ...
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bioRxiv - Immunology 2021Quote: ... Each dilution was incubated at 37°C and 5% CO2 for 1 hour with an equal volume of 1000 plaque forming units/ml (PFU/ml) of SARS-CoV-2 (isolate USA-WA1/2020) diluted in DMEM (Gibco) containing 2% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2021Quote: Biotinylated SARS-CoV-2 prefusion stabilized S trimer was incubated with yellow-green streptavidin-fluorescent beads (Molecular Probes, Eugene, OR) for 2 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... They were then co-transfected with 500 ng of IRF3-GFP and 500 ng of SARS-CoV-2 protein-expressing plasmid using Lipofectamine 2000 reagent (11668019, Invitrogen). 24 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: ... were cultured in the presence of SARS-CoV-2-S (9pmol) (PepTivator) in 24-well plates and maintained in IMDM medium (Gibco) supplemented with penicillin (100 units/mL) ...
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bioRxiv - Genetics 2022Quote: ... Genotyping was done using Axiom™ Human Genotyping SARS-CoV-2 Research Array as instructed by the manufacturer (Thermo Scientific).
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 D614G S ectodomain was produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2020Quote: ... 250,000 or 1000 SARS-CoV-2 viral copies were used to start the initial cDNA synthesis by SuperScript III reverse transcriptase (Invitrogen), using a mix of random primers ...
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bioRxiv - Microbiology 2021Quote: ... cells transfected with the SARS-CoV-2 S protein deletion mutants and controls were washed once with DPBS (Fisher Scientific), fixed with 4% (w/v ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from the supernatants of SARS-CoV-2-infected cells by using a PureLink RNA Mini Kit (Invitrogen). Thereafter ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 viral nucleocapsid protein (NP) was detected using the anti-NP protein antibody (Cat. # PA5-81794, Thermo Fisher) diluted 1:10000 in 0.1% tween-20/1%BSA/PBS solution as a primary antibody ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Cryopreserved PBMC from the SARS-CoV-2-infected participant and a SARS-naïve donor were thawed at 37°C and stained for SARS-CoV-2-specific memory B cells with a flow cytometry panel consisting of: a viability dye (7AAD, Invitrogen), CD14 PE-Cy5 ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
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bioRxiv - Immunology 2020Quote: ... The detection and measurement of SARS-CoV-2 viral load were performed using COVID-19 RT-PCR diagnostic kit with specific Taqman probes (ThermoFisher) of SARS-CoV-2-specific genes encoded by the RNA-dependent RNA polymerase (RdRp/ORF1ab) ...
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bioRxiv - Microbiology 2021Quote: ... and cDNAs were synthesized using SARS-CoV-2 nucleocapsid (N) reverse primer N660R (5’-AGCAAGAGCAGCATCACCGCCATTGCCAGC-3’) and M-MLV reverse transcriptase (Invitrogen). Then ...
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bioRxiv - Microbiology 2021Quote: ... assay using the OPTI Medical SARS-CoV-2 RT-PCR kit following the manufacturer’s instructions on an ABI 7500 Fast instrument (ThermoFisher Scientific). The OPTI Medical SARS-CoV-2 RT-PCR assay detects two different targets in the gene encoding viral nucleocapsid (N ...
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bioRxiv - Microbiology 2020Quote: ... One-step RT-qPCR was performed with primers targeting the SARS-CoV-2 N region (BEI) using the EXPRESS One-Step Superscript qRT-PCR Kit (ThermoFisher) on an Applied Biosystems QuantStudio3 instrument ...
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bioRxiv - Microbiology 2021Quote: ... Peptide identification of the human and SARS-CoV-2 proteome was performed by Proteome Discoverer (version 2.2, Thermo Fisher Scientific) using the SequestHT search engine as described (Seidel et al 2018) ...
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bioRxiv - Microbiology 2022Quote: ... total RNA from non-infected (Ctrl) or SARS-CoV-2 infected A549-hACE2 and hPCLS was isolated using Trizol (Invitrogen). RNA was treated with DNase (DNase-Free DNase Set ...
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bioRxiv - Microbiology 2022Quote: ... were transfected into Calu-3 cells using Dharmafect-4 transfection reagent (#cat: T-2004-02, Dharmacon) 48 hours prior to infection with SARS-CoV-2: siAAK1 (ID: s22494, Ambion), siBIKE (M-005071-01 ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 targeting probes were custom-made #6007037-01 directed against the ORF1a sequences between positions 401-1327 (ThermoFisher). Subsequently ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... and stained for SARS-CoV-2 N protein using Biotin-labelled-CR3009 antibody produced in-house together with a Streptavidin-Alexa488 (S32354, Invitrogen) and cellular DNA using DAPI (10236276001 ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 pseudovirus-based neutralization assay were performed in Nunc 96-well cell culture plates (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Neuroscience 2023Quote: Neurons from K18-hACE2 and WT mice were inoculated with SARS-CoV-2 isolate USA-WA1/2020 at 30 MOI in 100 µL Neurobasal A (Invitrogen) for 8-well chamber slides and 200 µL for 24-well plates for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Six- to 12-week-old male and female mice were inoculated by the intranasal route with 103 pfu of SARS-CoV-2 in a volume of 50ul PBS (Gibco). Prior to inoculation mice were anesthetized by inhalation of isoflurane ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Immunology 2023Quote: Complement sufficient NHS (0-10% final concentration) was mixed 103 PFU of SARS-CoV-2 and RNAse A (1mg/ml,Thermofisher) for 2 hr at 37°C ...
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A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
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bioRxiv - Immunology 2023Quote: Affinity purified SARS-CoV-2 (Wuhan) RBD was biotinylated using EZ-Link Sulfo-NHS-LC-biotin (Life Technologies, USA, A39257) accordingly to manufacturing protocol ...
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bioRxiv - Immunology 2024Quote: ... Serum serial dilutions were incubated with 2000 PFU of an early SARS-CoV-2 isolate in 100 µl OptiPro medium supplemented with 1x GlutaMAX (both Gibco) for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies (Invitrogen, 1:3000) were used for chemiluminescence detection through Thermo Scientific Pierce ECL.
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bioRxiv - Cancer Biology 2021Quote: ... horseradish peroxidase-(HRP) conjugated secondary antibody (Novex, Life technologies) at 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse horseradish peroxidase (HRP) (1:5000, Invitrogen #31430,), anti-rabbit horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-rabbit horseradish peroxidase (HRP) (1:5000, Invitrogen #31460,) used to develop respective blots ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary horseradish peroxidase (HRP) antibodies were obtained from Invitrogen. Pierce ECL Chemiluminescence kit (Thermo Fisher scientific #32106 ...
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bioRxiv - Neuroscience 2020Quote: ... conjugated to horseradish peroxidase followed by ECL (Thermo Scientific). Detection of membranes was performed with the Vilber Fusion Solo S system.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to horseradish peroxidase (Invitrogen, #32430, #32460) were used at 1:10,000 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies conjugated with horseradish peroxidase (Thermo Fisher Scientific) were incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies conjugated to horseradish peroxidase (HRP) (Thermo Fisher) or IR800 and IR680 dye (Li-Cor ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse IgG-Horseradish peroxidase (HRP) (Cat # 31430, Invitrogen), anti-rabbit IgG HRP (Cat # 31466 ...
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bioRxiv - Immunology 2020Quote: ... goat anti-mouse IgG conjugated to horseradish peroxidase (ThermoFisher), was then added ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies conjugated to horseradish peroxidase (Invitrogen, #32430, #32460) were used at 1:10,000 ...