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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and 1.0 mU/mL horseradish peroxidase (ThermoFisher) were supplemented into the cell culture media ...
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bioRxiv - Microbiology 2023Quote: ... Protein A conjugated to horseradish peroxidase (Invitrogen) was added for 2 hr at room temperature ...
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Loss of the mitochondrial citrate carrier, Slc25a1/CIC disrupts embryogenesis via 2-HydroxyglutaratebioRxiv - Developmental Biology 2024Quote: ... Appropriate horseradish peroxidase– conjugated secondary antibody (Invitrogen) was applied after incubation with primary antibody ...
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bioRxiv - Immunology 2021Quote: ... The proteins were transferred to nitrocellulose membrane and probed with polyclonal rabbit serum against SARS-CoV S1 domain (Thermofisher, Cat No. PA5-81798 and PA5-81795), monoclonal antibodies I1 (8G5F11 ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed three times for 5 min and incubated with 1:3000 dilution of horseradish peroxidase-conjugated Goat anti-mouse IgG Fc secondary antibody (Invitrogen) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... and SARS-CoV-2 were mixed in a ratio of 0.7:0.7:0.4 with Opti-MEM® media (Gibco) to give 275 μL ...
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bioRxiv - Microbiology 2022Quote: ... and spike protein expressing pcDNA3.1-SARS-CoV-2 SΔCT were co-transfected into HEK293T cells (ATCC CRL_3216) with lipofectamine 2000 (ThermoFisher Scientific). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Cell Biology 2021Quote: ... SARS-CoV-2 titration was obtained using a TaqMan 2019nCoV assay kit v1 RealTime-PCR (Thermo Fisher, Italy).
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bioRxiv - Biochemistry 2021Quote: The gene encoding the PLpro (also known as nsp3) protein from SARS-CoV-2 (YP_009725299.1) was also synthesized commercially (Invitrogen) and cloned into the pSUMO1 vector with N-terminal 8xHis conjugated with yeast SUMO ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: SARS-CoV-2 total viral RNA was extracted from Vero cells (ATCC CCL-81) cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2020Quote: Total RNA was extracted from Vero CCL81 cells infected with VSV-SARS-CoV-2-SΔ21 using Trizol (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Immunology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
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bioRxiv - Microbiology 2021Quote: ... 12 µL of SARS-CoV-2 stock virus (USA_WA1/2020; 8.3×104 pfu) in OptiMEM supplemented with 1x antibiotic/antimycotic (Gibco) was pipetted directly onto each surface being tested and spread with a pipette tip to facilitate efficient drying ...
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bioRxiv - Microbiology 2021Quote: ... and spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using Wuhan/WIV04/2019strain (GISAID accession ID ...
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bioRxiv - Immunology 2021Quote: ... and Spike protein expressing pcDNA3.1-SARS CoV-2 SΔCT of variants were co-transfected into HEK293T cells by lipofectamine 2000 (ThermoFisher). Pseudoviruses of SARS-CoV-2 variants were generated by using WA1/2020 strain (Wuhan/WIV04/2019 ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA of SARS-CoV-2 N-gene was synthesized with MEGAscript T7 (Thermo Fisher Scientific) using PCR product generated from SARS-CoV-2 WA1 cDNA and primers N0.5_F and N3-5_R (Eurofins ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 coding sequences were inserted using the Gateway LR Clonase II Enzyme Mix kit (Invitrogen, CA), yielding plasmids pLCPD-S ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... TH-tag 1:500 (chicken, Ab76442 or sheep, Invitrogen PA1-4679), diluted into blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with an anti-rabbit horseradish peroxidase-linked antibody for FtsZ and an antiguinea pig horseradish peroxidase-linked antibody for SepF (Invitrogen), both at 1:5000 dilution for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were probed with rabbit-anti-SARS spike (Invitrogen, PA1-411-1165), mouse-anti-SARS-CoV-2 spike (GeneTex 1A9) ...
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bioRxiv - Microbiology 2021Quote: ... Horseradish peroxidase conjugated secondary antibodies (anti-rabbit, Invitrogen 31460 ...
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bioRxiv - Cell Biology 2021Quote: ... horseradish peroxidase-tagged second antibody (Thermo Fisher Scientific) was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL ...
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bioRxiv - Plant Biology 2020Quote: ... Horseradish peroxidase (Thermo Fisher Scientific, Waltham, MA, USA) was dissolved in water at a concentration of 10 mg/ml ...
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bioRxiv - Cell Biology 2020Quote: ... labelled with horseradish peroxidase (Z 25054 Molecular Probes). The cells were then washed with PBS and fixed for 30min in 3% glutaraldehyde in 0.1M sodium cacodylate (NaCaC ...
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bioRxiv - Cell Biology 2021Quote: ... horseradish peroxidase conjugated secondary antibodies (Jackson Immunoresearch/Invitrogen) were added and proteins were detected with Immobilon Western chemiluminescent HRP substrate and a Fujifilm LAS-4000 Luminescence Image Analyser ...
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bioRxiv - Biochemistry 2022Quote: ... or antimouse horseradish peroxidase (Invitrogen, catalog no. 31340). The chemiluminescence signal from Clarity™ Western ECL substrate (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... or IgG2a or IgG2c–horseradish peroxidase conjugates (ThermoFisher) were used as secondary Abs ...
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bioRxiv - Neuroscience 2021Quote: ... and streptavidin horseradish peroxidase conjugated (HRP, Thermo Fisher). Secondary HRP antibodies (Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... Anti-mouse horseradish peroxidase-conjugated antisera (ThermoFisher Scientifics) was used as a source of the secondary antibody and detected with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... and horseradish-peroxidase-conjugated secondary antibodies (Thermo Fisher) were used as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... and 5 U/mL horseradish peroxidase (ThermoFisher #31491). 10 mM succinate was added to the incubation ...
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bioRxiv - Biophysics 2023Quote: ... Horseradish peroxidase (HRP)-linked conjugated secondary antibodies (Invitrogen) were developed with SuperSignal West Pico Chemiluminescent Substrate (ThermoScientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Horseradish peroxidase conjugated secondary antibodies (Invitrogen, Thermo Fisher) and enhanced chemiluminescence (Amersham ...
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bioRxiv - Molecular Biology 2023Quote: ... Horseradish peroxidase conjugated secondary antibodies (Invitrogen, Thermo Fisher) and enhanced chemiluminescence (Amersham ...
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bioRxiv - Developmental Biology 2023Quote: ... Horseradish peroxidase (HRP)–conjugated secondary IgG antibodies (Invitrogen, #31470 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... horseradish peroxidase (HRP) conjugated Rabbit Anti-Mouse (Invitrogen) was used 1:2000.
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: RNA extraction of SARS-CoV-2 infected A549-ACE2 cells was performed by TRIzol (Thermo Fisher Scientific, 15596-026) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 infected VERO cells were lysed by adding 0.3-0.4 mL of TRIzol Reagent (Invitrogen, ThermoFisher Scientific) per 1 × 105-107 cells directly in the culture dish and pipetting up and down several times to homogenize ...