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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... and either pMD2.G or pTwist-EF1alpha-SARS-CoV-2-S-2xStrep using LTX (Invitrogen) transfection reagents according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 infected cells were washed with PBS and lysed with TRIzol reagent (Invitrogen). Total RNA was extracted using RNeasy mini kit (QIAGEN ...
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bioRxiv - Immunology 2024Quote: ... indicated SARS-CoV-2 Z-RNAs) was incubated with Lipofectamine 2000 transfection reagent (Invitrogen, 11668019), for 15 mins at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of N501Y-SARS-CoV-2 BAC were transfected into Vero E6 (ATCC) with Lipofectamine 3000 (Invitrogen) in a 6-well plate according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Binding studies in HEK 293T cells were performed using rVSV-SARS-CoV-2 Spike virions (rVSV/Spike) and binding was performed at room temperature in IMMULON 2HB flat bottom plates (Thermo Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: Viral RNA from SARS-CoV-2 infected HBEC cell lysates was extracted using TRIzol (Life Technologies) and purified using Direct-zol RNA MiniPrep Plus according to manufacturer’s instructions (Zymo Research ...
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bioRxiv - Molecular Biology 2020Quote: ... complementary cDNA of SARS-CoV-2 clinical samples was generated using SuperScript II Reverse Transcriptase (Invitrogen), random primers and 10μL of RNA ...
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bioRxiv - Bioengineering 2021Quote: Both Nefmut/SARS-CoV-2-based fusion proteins were cloned into the pVAX1 plasmid (Thermo Fisher) as already described (22) ...
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bioRxiv - Microbiology 2021Quote: ... The purified bacmid of SARS-CoV-2 was used for transfection using cellfectin (Thermo Fisher, SG). The viral stocks obtained from transfection were amplified and expressed for protein production ...
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bioRxiv - Cell Biology 2021Quote: ... employing the Ion AmpliSeq SARS-CoV-2 RNA custom primers panel (ID: 05280253, Thermo Fisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: Artificial codon optimized genes encoding SARS-CoV-2 nsp10 and nsp16 proteins were commercially synthesized (Invitrogen) and cloned in a pSUMO vector that encodes an N-terminal His8x-SUMO tag ...
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bioRxiv - Immunology 2020Quote: ... We synthesized oligonucleotide fragments encoding the SARS-CoV-2 gene using GeneArt strings (Thermo Fisher Scientific) and assembled into the plasmid vector with NEB HiFi assembly (New England BioLabs) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR detection of SARS-CoV-2 was performed on a QuantStudio 5 instrument (Applied Biosystems) using a TaqPath 1-step RT-qPCR Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... An anti-S2 SARS-CoV-2 S polyclonal primary antibody (1:1,500 dilution,Invitrogen PA5-114534) and an Alexa Fluor 680-conjugated goat anti-rabbit secondary antibody (1:20,000 dilution ...
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bioRxiv - Immunology 2020Quote: ... and a TwinStrepTag (SARS-CoV-2 HexaPro S) were transiently transfected into FreeStyle293F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2021Quote: A commercially available SARS-CoV-2 Inhibitor Screening Kit (Cat#: 16605302, Fisher Scientific GmbH, Schwerte, Germany) was used for cell free detection of SARS-CoV-2 Spike – ACE2 interaction inhibition ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 RT-qPCR was performed in a 7500 Real-Time PCR System (Applied Biosystems) using Seegene-Allplex 2019-nCoV Assay ...
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bioRxiv - Immunology 2021Quote: ... incubated with SARS-CoV-2 Nucleoprotein polyclonal antibody at 1:500 dilution (Invitrogen, Carlsbad, CA, USA) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and Jurkat cells with stable SARS-CoV-2 S expression were labeled with CFSE dye (ThermoFisher). 75,000 Jurkat cells were added to macrophage cells in a 96-well plate in the presence of 2G1 or the isotype control antibody ...
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bioRxiv - Genomics 2021Quote: Total RNA from SARS-CoV-2 infected cell samples was extracted with Trizol reagent (Invitrogen, 15596026), and then subjected to the rRNA depletion with the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat ...
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bioRxiv - Microbiology 2022Quote: ... except anti-SARS-CoV-2 ORF3a antibody was used (1:500; PA5-116946; Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 ORF7a was cloned into the MaMTH Prey vector using Gateway cloning technology (ThermoFisher). The correctness of new constructs was confirmed by Sanger sequencing (Eurofins).
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Immunology 2022Quote: ... SOLAμ horseradish peroxidase (HRP) 2 mg/1 ml 96-well plate (Thermo Scientific) was used to concentrate the peptides (according to the manufacturer’s instructions) ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: Cultured SARS-CoV-2 was used to prepare virus stocks in minimum essential medium (MEM 2%) (Gibco, Thermo Fisher Scientific) containing 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2019Quote: ... The plate was incubated with detection antibody (mouse anti-human IgG1 Fc conjugated to horseradish peroxidase clone HP6069; ThermoFisher) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: RNA from SARS-CoV-2-infected organoids was extracted by adding 750 μl TRIzol (Thermo Fisher Scientific), incubating at 55 °C for 5 min and then adding 150 μl chloroform ...
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bioRxiv - Immunology 2021Quote: ... Immunohistochemistry (IHC) was performed using a monoclonal antibody specific to SARS-CoV-2 Nucleocapsid (clone B46F; ThermoFisher) per previously reported methods [30] ...
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bioRxiv - Immunology 2021Quote: ... lung tissue of mice challenged with SARS-CoV-2 was homogenized in TRIzol reagent (Life Technologies, 15596018) and total RNA was isolated using a PureLink RNA Mini kit (Life Technologies ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in Vero E6 cells in Opti-MEM I (Invitrogen, Cat. # 51985091) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... ChAdOx1 SARS CoV-2 S viral vectors were rescued in T-REx™ cells (Invitrogen, Cat. R71007), a derivative of HEK293 cells which constitutively express the Tet repressor protein and prevent antigen expression during virus production ...
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bioRxiv - Microbiology 2020Quote: ... Gene specific primers to SARS-CoV-2 (Wuhan v1, NSP14) and SYBR green master mix (Applied Biosystems) were used to amplify viral RNA and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT-PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2021Quote: The SARS-CoV-2 S ectodomains were produced in 200 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A variant-specific RT-qPCR with the TaqMan™ SARS-CoV-2 kit Mutation Panel (Applied Biosystems) was used to screen the VOC/VOI mutations S_L452R and S_T478K following manufacturer ‘s protocol.
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bioRxiv - Microbiology 2021Quote: Mouse tissue from SARS-CoV-2 infected mice was homogenized using glass beads in TRIzol Reagent (Invitrogen). Equal volume of 100% EtOH was mixed with the TRIzol homogenate and processed using Direct-Zol RNA MiniPrep Kit (Zymo ...
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bioRxiv - Microbiology 2020Quote: Milling of SARS-Cov-2 infected cells was carried out using a Scios DualBeam cryoFIB (ThermoFisher Scientific) equipped with a PP3010T transfer system and stage (Quorum Technologies) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 S protein-expressing plasmids were transfected into HEK293T cells using Lipofectamine 3000 (Invitrogen, USA). 24 h after transfection ...
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bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolates were propagated in VeroE6 cells in Opti-MEM I (Invitrogen, Carlsbad, CA, USA) containing 0.3% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: The SARS-CoV-2 S ectodomains were produced in 100 mL cultures of Expi293F Cells (ThermoFisher Scientific) grown in suspension using Expi293 Expression Medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Primary antibodies used in immunohistochemical assays with dilutions: SARS-CoV-2 nucleocapsid (Invitrogen #PA5-81794; 1:1000), CD3ɛ (CST #99940 ...
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bioRxiv - Bioengineering 2023Quote: ... Gene-specific primers to SARS-CoV-2 (Wuhan v1, NSP14) and SYBR green master mix (Applied Biosystems) were used to amplify viral RNA ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... SARS-CoV-2 quantification was performed employing the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Inc.) with indicated primers (Table 1) ...
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bioRxiv - Biochemistry 2021Quote: Four rounds of panning were used to isolate scFvs binding both MERS-CoV S2 and SARS-CoV-2 spike using the following solutions coated on high binding plates: 2 μg/mL anti-c-myc tag antibody (Invitrogen) to eliminate phage expressing no or truncated scFv (Round 1) ...
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bioRxiv - Molecular Biology 2020Quote: ... the membrane was incubated with a mouse secondary antibody conjugated with horseradish peroxidase (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... and Horseradish Peroxidase (HRP, Life Technologies, #31490) as described at Rodriguez et al [23] ...
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bioRxiv - Microbiology 2021Quote: ... Horseradish peroxidase (HRP)-conjugated secondary antibodies (ThermoFisher) were used at the concentrations shown in parentheses ...
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bioRxiv - Microbiology 2022Quote: ... and horseradish peroxidase-conjugated secondary antibodies (Invitrogen). Immunoblots were developed with SuperSignal West Femto substrate (Thermo Fisher Scientific Corp. ...