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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding the heavy and light chains of antibodies were transfected into Expi293F cells using an ExpiFectamine 293 transfection kit (Gibco) in a 2:1 mass ratio for IgG or a 1:1 mass ratio for Fab following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... We measured CO2 concentration and 13C signature of gas samples using a headspace gas sampler (GasBench II, Thermo Fisher Scientific, Bremen, Germany) coupled to an isotope ratio mass spectrometer (Delta V Advantage ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and supplies were purchased from Fisher Scientific (Suwanee, GA). All graphs and statistical analyses were done with the Prism software package (GraphPad Inc. ...
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bioRxiv - Microbiology 2024Quote: ... all chemicals were procured from Fisher Scientific (Atlanta, GA), VWR International (Pittsburgh ...
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then suspended in antibody binding buffer and split for overnight incubation with antibodies specific to INTS12 (Thermo Fisher, 16455-1-AP) (Figure 5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The ovaries were collected in oocyte manipulating media [alpha Minimum Essential Medium alpha (αMEM) + GlutaMax media (Gibco, Life Technologies, Carlsbad, CA) supplemented with 25 mM HEPES (Gibco)] and punctured repeatedly with a 30-guage needle to release the cumulus-oocyte complexes (COCs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The ovaries were collected in oocyte manipulating media [alpha Minimum Essential Medium alpha (αMEM) + GlutaMax media (Gibco, Life Technologies, Carlsbad, CA) supplemented with 25 mM HEPES (Gibco)] and punctured repeatedly with a 30-guage needle to release the cumulus-oocyte complexes (COCs) ...
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bioRxiv - Bioengineering 2023Quote: ... and CD90 were isolated from subcutaneous fat of adult NGL mice and cultured in Minimum Essential Medium Alpha (alpha-MEM; Mediatech, Inc.) containing 10% fetal bovine serum (FBS; Life Technologies) as previously described.22,30 Macrophages were prepared from bone marrow monocytes of adult NGL mice and cultured in a macrophage culture medium containing 10% conditioned medium from L929 cell culture ...
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bioRxiv - Immunology 2022Quote: ... were transfected at cell densities between 8.0×105 to 1.0×106 cells/ml with equal masses of plasmids containing the heavy chain and light chain genes using FreeStyleTM MAX Reagent (ThermoFisher Scientific 16447100); both plasmids were diluted in OptiPROTM SFM (ThermoFisher Scientific 12309050) ...
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bioRxiv - Microbiology 2021Quote: ... The antibody production was conducted by co-transfection of the light chain and heavy chain sequences into Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific, A14635). The culture supernatant was collected at 7 days post-transfection ...
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bioRxiv - Immunology 2020Quote: ... Four µg of the heavy chain and 4 µg of the corresponding light chain were mixed together in 375 µl Opti-MEM (Gibco, ThermoFisher Scientific, UK) before addition of polyethylenimine (PEI MAX (Polysciences ...
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bioRxiv - Immunology 2023Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 11D5-3 single chain variable fragment (scFv)3 and the human heavy-chain-only FHVH3316 were codon-optimized and synthesized by GeneArt (Thermo Fisher Scientific). Retroviral constructs encoding APRIL CARs ...
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bioRxiv - Immunology 2024Quote: ... The plasmids were transiently co-transfected into ExpiCHO cells at a ratio of 2:1 (heavy chain to light chain) using ExpiFectamine CHO Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-alpha-Tubulin AA4.3-c (1:5000; DSHB)) were detected using HRP-conjugated secondary antibodies (1:10000; Thermo Fisher) and ECL Prime kit (Amersham) ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used: mouse monoclonal anti-CaMKII alpha (1/100 dilution, ThermoFisher, Cat#MA1-048, RRID: AB_325403), rabbit polyclonal anti-mCherry (1/200 dilution ...
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bioRxiv - Immunology 2021Quote: ... High-binding 96-well plates (Nunc) were coated with the appropriate antigen (S1 protein 1.2 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... in Annexin V binding buffer (Invitrogen) at a concentration of 5 × 106 cells/mL for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... High-binding ELISA plates (Thermo Scientific) were coated with capture antibody and incubated overnight at room temperature ...
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bioRxiv - Immunology 2021Quote: High binding Immunlon HBX plates (ThermoFisher) were coated with 2.5μg/ml of recombinant P ...
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bioRxiv - Immunology 2020Quote: ... MaxiSorp high binding ELISA plates (Nunc) were coated with 100 μL/well of 1 μg/mL recombinant SARS-CoV-2 protein in PBS ...
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bioRxiv - Molecular Biology 2020Quote: Microtiter High Binding plate (Nunc MaxiSorp) wells were coated with 30 nM of His-tagged DNMT1 (Active Motif ...
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bioRxiv - Immunology 2021Quote: ... MaxiSorp high binding ELISA plates (Nunc) were coated with 100 μL per well of 1 μg mL−1 recombinant SARS-CoV-2 protein with the pre-fusion stabilized conformation in PBS ...
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bioRxiv - Immunology 2020Quote: ... high-binding microtiter plates (Nunc, Inc.) were first coated with D7324 antibody (Aalto Bio Reagents ...
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bioRxiv - Microbiology 2022Quote: ... assayed by dye binding (Qubit, Invitrogen / Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... high-binding 96-well plates (Nunc) with 30 µl fetuin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... MaxiSorp high-binding ELISA plates (NUNC) were coated with 10μg/mL Spike protein in PBS (50mL per well) ...
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bioRxiv - Biochemistry 2023Quote: ... high-binding 96-well plates (Nunc) were coated with 2 µg/mL neutravidin and blocked with buffer containing 1% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... MaxiSorp high binding ELISA plates (Nunc) were coated with 100□μl/well of 1□μg/ml highly purified SARS-CoV-2 SARS-CoV-2 RBDs ...
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bioRxiv - Bioengineering 2024Quote: ... High-binding MaxiSorp plates (Thermo Fisher) were coated with OVA at 10 μg/mL (for samples ...
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bioRxiv - Neuroscience 2021Quote: ... Protein-DNA-antibody complexes were precipitated with protein G-magnetic beads (Invitrogen) for 1hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Antibody/protein mixture was added to 50 μL Dynabeads Protein A (ThermoFisher) and incubated end-over-end for one hour at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein–antibody complexes were bound to magnetic protein G beads (Life Technologies) for 4–5 h and washed with standard IP wash buffers for 10 min at 4°C ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: The wild-type copy of the xopX gene and its 14-3-3 protein binding motif mutants were cloned in the yeast two-hybrid vector pDEST32 (Invitrogen) using the Gateway cloning system (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... to create a fusion to the Maltose-binding protein (MBP) or into pDEST15 for fusions to Glutathion-S-transferase (GST) (ThermoFisher). To create GUS reporter constructs ...
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bioRxiv - Microbiology 2020Quote: ... gH/gL or gL (diluted in PBS; 100 µl/well) were coated overnight on Nunc MaxiSorp high protein-binding capacity ELISA plates (ThermoFisher). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... truncation and a TEV protease cleavage site followed by a double maltose-binding protein-(MBP) tag at its C terminus was cloned into the pFastBac vector (Invitrogen). To obtain a SV1–Gs complex with good homogeneity and stability ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were measured by BCA and samples were diluted to 1 mL of 0.5 mg/mL in RIPA buffer in low protein binding collection tubes (Thermo Fisher) containing 25 μL of NanoLink® magnetic streptavidin beads ...
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bioRxiv - Molecular Biology 2023Quote: The coding sequence for the maltose binding protein (MBP) with a recognition site for 3C protease was amplified by PCR and cloned into pFastBac-HTb (Invitrogen) (pFastBac-HTb-MBP-3C) ...
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bioRxiv - Neuroscience 2024Quote: ... Cleared supernatant was diluted 1:1 with IgG binding buffer and passed through 1 ml of protein A/G agarose bead slurry (ThermoFisher 20421 ...
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bioRxiv - Microbiology 2024Quote: The binding of KIRs-Fc fusion proteins to iRBCs was assessed by flow cytometric analysis with Attune NxT (ThermoFisher Scientific). Prior to the assay ...
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bioRxiv - Immunology 2022Quote: ... Antibody-coupled Dynabeads Protein G (Thermo Fisher) were used for immunoprecipitations performed overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein A (polyclonal antibodies, Thermo Scientific, 20333) or Protein G (monoclonal antibodies ...
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bioRxiv - Genetics 2024Quote: ... antibodies coated to Protein A Dynabeads (Invitrogen). Real-time quantitative PCR was performed using iTaq universal SYBR Green (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... goat anti-human IgM u chain (Invitrogen, Thermofisher Scientific), for detecting immunoglobulins in human serum together with goat anti-mouse IgG-AlexaFluor 568 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... or anti clathrin Heavy Chain (MA1-065, Thermo Fisher) at 1 μg mL−1 in PBS blocking buffer with 5% BSA and 0.01 % w/v saponin ...
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bioRxiv - Plant Biology 2022Quote: Polymerase chain reactions utilized DreamTaq polymerase (ThermoFisher, Waltham, MA) and the supplied reaction buffer components ...